Effects of exogenous amino acid applications on the plant-parasitic nematode Heterodera schachtii

Summary Amino acid (AA) applications have been reported to affect plant-parasitic nematodes. Here, we analysed the effects of methionine (Met), lysine (Lys), threonine (Thr), isoleucine (Ile), homoserine (Hom) and tryptophan (Trp) on the sedentary plant-parasitic nematode, Heterodera schachtii, under in vitro conditions. No AA showed direct effects on the activity of infective second-stage juveniles (J2) of H. schachtii. Soaking J2 in Lys for 24 h increased the number of developing females and reduced the number of males. Thr treatments reduced the total number of nematodes developing in the host plant. The strongest effects were observed when AA were added to the nutrient medium in a monoxenic Arabidopsis thaliana culture. Ile, Met or Thr clearly reduced the number of female nematodes developing in the host plant. These AA are direct metabolic derivatives of Hom. Direct effects on pre-infective J2 can be differentiated from effects that may involve the host plant.

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Identification of potential host plant mimics of CLAVATA3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtii

Molecular Plant Pathology ◽

10.1111/j.1364-3703.2010.00660.x ◽

2010 ◽

Vol 12 (2) ◽

pp. 177-186 ◽

Cited By ~ 64

Author(s):

JIANYING WANG ◽

AMY REPLOGLE ◽

RICHARD HUSSEY ◽

THOMAS BAUM ◽

XIAOHONG WANG ◽

...

Keyword(s):

Host Plant ◽

Parasitic Nematode ◽

Plant Parasitic Nematode ◽

Heterodera Schachtii ◽

Potential Host ◽

Plant Parasitic

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Diversity and incidence of plant-parasitic nematodes associated with saffron (Crocus sativus L.) in Morocco and their relationship with soil physicochemical properties

Nematology ◽

10.1163/15685411-00003286 ◽

2019 ◽

Vol 22 (1) ◽

pp. 87-102 ◽

Cited By ~ 1

Author(s):

Fouad Mokrini ◽

Salah-Eddine Laasli ◽

Youssef Karra ◽

Aicha El Aissami ◽

Abdelfattah A. Dababat

Keyword(s):

Physicochemical Properties ◽

Parasitic Nematode ◽

Management Strategies ◽

Plant Parasitic Nematode ◽

Crocus Sativus ◽

Parasitic Nematodes ◽

Soil Physicochemical Properties ◽

Plant Parasitic Nematodes ◽

Starting Point ◽

Plant Parasitic

Summary Saffron (Crocus sativus) fields in Morocco’s Taliouine and Taznakht regions were surveyed between January and April 2018 to study the diversity and incidence of plant-parasitic nematodes and assess the effects of soil physicochemical properties on the nematodes. Fourteen nematode genera were identified in soil and root samples collected from 66 saffron fields. The most common plant-parasitic nematodes in the Taliouine region were Pratylenchus spp. and Helicotylenchus spp. In the Taznakht region, the most common nematodes were Pratylenchus spp., Tylenchorhynchus spp. and Ditylenchus dipsaci. Nematodes, particularly Pratylenchus spp. and Ditylenchus spp., were abundant and frequent throughout the region. Several nematode genera were significantly associated with soil texture and mineral content, indicating that soil properties play an important role in plant-parasitic nematode communities. This description of plant-parasitic nematode assemblages associated with saffron fields in Morocco and their relationship with soil physicochemical properties provides a starting point from which appropriate nematode management strategies can be implemented.

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Molecular Characterization and Phylogenetic Relationships of Plant-Parasitic Nematodes Associated with Turfgrasses in North Carolina and South Carolina, United States

Plant Disease ◽

10.1094/pdis-10-14-1060-re ◽

2015 ◽

Vol 99 (7) ◽

pp. 982-993 ◽

Cited By ~ 5

Author(s):

Yongsan Zeng ◽

Weimin Ye ◽

James Kerns ◽

Lane Tredway ◽

Samuel Martin ◽

...

Keyword(s):

North Carolina ◽

South Carolina ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

Parasitic Nematode ◽

Nematode Species ◽

Plant Parasitic Nematode ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Plant Parasitic

The near-full-length 18S ribosomal DNA (rDNA) gene and internal transcribed spacer 1 region were amplified and sequenced from 52 nematode populations belonging to 28 representative species in 13 families recovered from turfgrasses in North Carolina (38 populations) and South Carolina (14 populations). This study also included 13 nematode populations from eight other plant hosts from North Carolina for comparison. Nematodes were molecularly characterized and the phylogenetic relationships were explored based on 18S rDNA sequences. Phylogenetic analysis using Bayesian inference was performed using five groups of the plant-parasitic nematode populations Tylenchids, Criconematids, Longidorids, Xiphinematids, and Trichodorids. The 65 nematode populations were clustered correspondingly within appropriate positions of 13 families, including Belonolaimidae, Caloosiidae, Criconematidae, Dolichodoridae, Hemicycliophoridae, Hoplolaimidae, Heteroderidae, Longidoridae, Meloidogynidae, Paratylenchidae, Pratylenchidae, Telotylenchidae, and Trichodoridae. This study confirms previous morphological-based identification of the plant-parasitic nematode species found in turfgrasses and provides a framework for future studies of plant-parasitic nematodes associated with turfgrasses based upon DNA sequences and phylogenetic relationships.

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Use of Entomopathogenic Nematodes and Thyme Oil to Suppress Plant-Parasitic Nematodes on English Boxwood

Plant Disease ◽

10.1094/pd-90-0471 ◽

2006 ◽

Vol 90 (4) ◽

pp. 471-475 ◽

Cited By ~ 7

Author(s):

Enrique E. Pérez ◽

Edwin E. Lewis

Keyword(s):

Population Growth ◽

Entomopathogenic Nematodes ◽

Parasitic Nematode ◽

Nematode Species ◽

Plant Parasitic Nematode ◽

Nematode Population ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Thyme Oil ◽

Plant Parasitic

A 2-year experiment was conducted to test suppression of plant-parasitic nematodes on English boxwood using entomopathogenic nematodes and 3.5% thyme oil formulated as Promax. Treatments were Steinernema riobrave formulated as BioVector and S. feltiae formulated as Nemasys, both applied at a rate of 2.5 billion infective juveniles/ha, thyme oil at rate of 9.3 liters/ha, and nontreated control. In the 2001 season, treatment with S. feltiae reduced (P ≤ 0.05) the population growth of Tylenchorhynchus sp. 7 days after treatment and Hoplolaimus sp. 30 and 60 days after treatment. Treatment with S. riobrave reduced (P ≤ 0.05) the population growth of all plant-parasitic nematode species at all sampling dates, with the exception of Mesocriconema sp. 30 days after treatment and Tylenchorhynchus sp. and Rotylenchus buxophilus 60 days after treatment. Treatment with thyme oil reduced (P ≤ 0.05) the population growth of all plant-parasitic nematode genera at all sampling dates except Tylenchorhynchus sp. and R. buxophilus 60 days after treatment. In the 2002 season, treatment with S. feltiae had no effect on nematode population growth. Treatment with S. riobrave reduced (P ≤ 0.05) the population growth of R. buxophilus 7 days after treatment, and all plant-parasitic nematodes 30 and 60 days after treatment except Hoplolaimus sp. 30 days after treatment and Mesocriconema sp. 60 days after treatment. Treatment with thyme oil reduced (P ≤ 0.05) the population growth at all sampling dates of plant-parasitic nematodes except Mesocriconema sp. 60 days after treatment.

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The application of microbial originating bioregulators to obtain in vitro lines of potato with increased resistance to parasitic nematodes

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v26.1281 ◽

2020 ◽

Vol 26 ◽

pp. 287-292

Author(s):

O. M. Shysha ◽

S. I. Spivak ◽

V. A. Tsygankova ◽

G. O. Iutynska ◽

L. O. Biliavska ◽

...

Keyword(s):

Parasitic Nematode ◽

Solanum Tuberosum L ◽

Heterodera Schachtii ◽

Parasitic Nematodes ◽

Wheat Seedlings ◽

Free System ◽

Potato Plants ◽

Organogenesis In Vitro ◽

Potato Resistance

Aim. Obtaining in vitro new lines of potato (Solanum tuberosum L.) cultivar Vernissage with genetically mediated resistance to the parasitic nematode H. schachtii on nutrient MS media with microbial bioregulators. Methods. In vitro conditions the process of organogenesis of potato plants on nutrient MS media containing microbial bioregulators used at concentrations 25-100 μl/l in combination with phytohormones 2 mg/l BAP and 0,1 mg/l NAA was investigated. Using dot blotting method the degree of hybridization between cytoplasmic si/miRNA isolated from cells of potato plants-regenerants, grown on the artificial invasive background, and nematode mRNA was studied. In the wheat seedlings cell-free system in vitro the silencing activity of si/miRNA isolated from cells of potato plants-regenerants on the template of nematode mRNA was investigated. Results. The experiments conducted in vitro conditions showed that the addition of microbial bioregulators at concentrations 25-100 μl/l in combination with phytohormones 2 mg/l BAP and 0,1 mg/l NAA into MS media increased the efficiency of regeneration of potato shoots to 43–47 % as compared with similar indices obtained on control MS media. The increase of the degree of hybridization to 19-38 % between cytoplasmic mRNA isolated from cells of nematode H. schachtii and si/miRNA isolated from cells of experimental potato plants-regenerants grown in vitro conditions on nutrient media containing bioregulators on the artificial invasive background was shown. Conclusions. Using microbial bioregulators in vitro conditions as components of nutrient MS medium increases potato shoot regeneration efficiency and enhances RNAi-mediated resistance of plants-regenerants to parasitic nematode H. schachtii. Keywords: microbial bioregulators, potato organogenesis in vitro, potato resistance to nematode Heterodera schachtii, RNA interference.

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Description of two new Ogma Southern, 1914 species (Nemata: Criconematoidea) with a list of plant-parasitic nematode species from Natal

Koedoe ◽

10.4102/koedoe.v35i1.385 ◽

1992 ◽

Vol 35 (1) ◽

Cited By ~ 1

Author(s):

Esther Van den Berg

Keyword(s):

National Park ◽

Nature Reserve ◽

Parasitic Nematode ◽

Nematode Species ◽

Plant Parasitic Nematode ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Tail Tip ◽

Plant Parasitic

Two new O^nia Southern, 1914 species are described and figured from Giant's Castle Nature Reserve, Kamberg Nature Reserve and the Royal Natal National Park. O^ma naomiae spec. nov. females are characterised by having 10 to 12 longitudinal rows of scales on 63 to 69 retrorse body annuli; scales haphazardly arranged on first nine to 10 annuli; scales mostly with two projections, becoming longer toward tail end; all scales with minute protuberances on outer edges; lip region with one annulus with a greater diameter than first body annulus. Juveniles with 14 to 16 longitudinal rows of scales on 73 to 76 retrorse body annuli; each scale bearing five to seven sharply pointed processes; one lip annulus with an equal diameter to first body annulus. O^ma ueckermanni spec. nov. females are characterised by eight longitudinal rows of rounded scales on 60 to 67 body annuli becoming longer toward tail tip where they bear two, three or four-pronged processes; roughly sculptured recessed part of annulus extends to follow the outline of the scale almost forming a pillar between two succeeding scales; one lip annulus with a greater diameter than first body annulus; raised labial area with six pseudolips and no submedian lobes; margin of lip annulus with rounded tooth-like projections; stylet 49,3 to 60,7 ^m long. A list is given of the 16 known plant-parasitic nematodes found in these areas.

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PPNEMA: A Resource of Plant-Parasitic Nematodes Multialigned Ribosomal Cistrons

International Journal of Plant Genomics ◽

10.1155/2008/387812 ◽

2008 ◽

Vol 2008 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Francesco Rubino ◽

Amalia Voukelatou ◽

Francesca De Luca ◽

Carla De Giorgi ◽

Marcella Attimonelli

Keyword(s):

Parasitic Nematode ◽

Plant Parasitic Nematode ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Parasitic Nematodes ◽

Agricultural Pests ◽

Plant Parasitic Nematodes ◽

Rna Sequences ◽

Complex Species ◽

Plant Parasitic

Plant-parasitic nematodes are important pests of crop plants worldwide, and also among the most difficult animals to identify. Their identification based on nuclear ribosomal DNA (rDNA) cistron (18S, 28S, and 5.8S RNA genes, and internal transcribed spacers, ITS1 and ITS2) is becoming a popular tool. Sequences from nuclear ribosomal RNA repeats have been used to demonstrate the identity of isolates from various hosts and to unravel the relationships of cryptic and complex species. In addition, the availability of RNA sequences allows study of phylogenetic relationships between nematodes, also for more complete understanding of their biology as agricultural pests. PPNEMA is a plant-parasitic nematode bioinformatic resource. It consists of a database of ribosomal cistron sequences from various species grouped according to nematode genera, and a search system allowing data to be extracted according to both text and pattern searching. PPNEMA offers to the scientific community a preprocessed archive of plant parasitic nematode sequences useful for nematologists. It is a tool to retrieve plant nematode multialigned sequences for phylogenetic studies or to recognize a nematode by comparing its rDNA sequence with the PPNEMA available genus specific multialignments.

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Preparation and sequencing of secreted proteins from the pharyngeal glands of the plant parasitic nematode Heterodera schachtii

Molecular Plant Pathology ◽

10.1046/j.1464-6722.2001.00078.x ◽

2001 ◽

Vol 2 (5) ◽

pp. 297-301 ◽

Cited By ~ 30

Author(s):

Jan De Meutter ◽

Bartel Vanholme ◽

Gino Bauw ◽

Tom Tytgat ◽

Greetje Gheysen ◽

...

Keyword(s):

Parasitic Nematode ◽

Plant Parasitic Nematode ◽

Heterodera Schachtii ◽

Secreted Proteins ◽

Plant Parasitic

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Survey of Plant-Parasitic Nematodes Associated with Yams in Ogun and Osun States of Nigeria

Journal of Plant Protection Research ◽

10.2478/v10045-008-0051-4 ◽

2008 ◽

Vol 48 (4) ◽

pp. 421-428 ◽

Cited By ~ 1

Author(s):

Ayodele Adegbite ◽

Jelili Saka ◽

Gideon Agbaje ◽

Felix Osuloye

Keyword(s):

Local Government ◽

Random Sampling ◽

Parasitic Nematode ◽

Soil Samples ◽

Plant Parasitic Nematode ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Ogun State ◽

Plant Parasitic

Survey of Plant-Parasitic Nematodes Associated with Yams in Ogun and Osun States of NigeriaA survey was conducted to determine the types, frequency and population of plant parasitic nematodes associated with the soils and roots of Yam (Dioscoreaspecies) in all the Local Government Areas of Ogun and Osun States of Nigeria using random sampling soil and root and pie pan modification of Baerman funnel for plant parasitic nematode extraction. Ten and nine genera of plant parasitic nematodes were encountered both from the soils and root samples from the two States. Plant parasitic nematodes recovered includedScutellonemaspp.,Meloidogynespp.,Pratylenchusspp.,Trichodorusspp.,Helicotylenchusspp.,Radopholusspp.,Longidorusspp.,Xiphinemaspp.,Rotylenchulusspp andAphelenchoidesspecies.Scutellonemaspp.,Meloidogynespp., andPratylenchusspp were most widely distributed with frequency ratings of 70, 65 and 60% respectively in soil samples from Ogun State and in the root samples the three genera predominated with 60, 55 and 45% frequency ratings respectively.Meloidogynespp.,Scutellonemaspp., andPratylenchusspp were most widely distributed with frequency ratings of 65, 45 and 35% respectively in soil samples from Osun State and in the root samples the three genera predominated with 55, 35 and 35% frequency ratings respectively.

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Predatory nematodes as bio-control agent against plant-parasitic nematode-A review

Agricultural Reviews ◽

10.18805/ag.r-1715 ◽

2018 ◽

Vol 38 (01) ◽

Cited By ~ 1

Author(s):

Gitanjali Devi ◽

Jisna George

Keyword(s):

Significant Role ◽

Parasitic Nematode ◽

Control Agent ◽

Plant Parasitic Nematode ◽

Parasitic Nematodes ◽

Feeding Apparatus ◽

Future Prospects ◽

Plant Parasitic Nematodes ◽

Taxonomic Categories ◽

Plant Parasitic

Nematodes are highly diversified and most abundant metazoans present in soil. Phytophagous nematodes attack plants cause enormous losses.The predaceous nematodes play a significant role in regulating the population of plant- parasitic nematodes. Based on type of feeding apparatus and mode of feeding, predatory nematode are classified into different taxonomic categories. This review has made an attempt to evaluate the bio-efficacy of predaceous nematodes and their future prospects.

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