The thermogenic and metabolic responses to photoperiod manipulations in Apodemus chevrieri

2013 ◽  
Vol 63 (3) ◽  
pp. 241-255 ◽  
Author(s):  
Wan-long Zhu ◽  
Lin Zhang ◽  
Zheng-kun Wang
Keyword(s):  
Body Mass ◽  
Uncoupling Protein ◽  
Resting Metabolic Rate ◽  
Mitochondrial Protein ◽  
Short Photoperiod ◽  
Uncoupling Protein 1 ◽  
Mitochondrial Uncoupling ◽  
Adipose Tissues ◽  
Serum Leptin ◽  
Brown Adipose

Environmental cues play important roles in the regulation of an animal’s physiology and behavior. In the present study, we examined the effects of short photoperiod on body weight as well as on several physiological, hormonal, and biochemical measures indicative of thermogenic capacity to test our hypothesis that short photoperiod stimulates increases in thermogenesis without cold stress in Apodemus chevrieri. A. chevrieri were randomly assigned to either a long or short photoperiod for 4 weeks at constant temperature. The short photoperiod group of A. chevrieri showed increases in resting metabolic rate and nonshivering thermogenesis during the 4-week photoperiod acclimation. At the end, A. chevrieri at short photoperiod had lower body weights, higher levels of mitochondrial protein content and cytochrome C oxidase activity in liver and brown adipose tissues, and had higher levels of mitochondrial uncoupling protein-1 contents in brown adipose tissues. No difference in serum leptin levels were found between short and long photoperiod groups, but serum leptin levels were positively correlated with body mass and body fat mass, and negatively correlated with energy intake and uncoupling protein-1 content in brown adipose tissues, respectively. All results suggest that the short photoperiod may induce an increased thermogenesis capacity in A. chevrieri and that leptin is potentially involved in the photoperiod induced body mass regulation and thermogenesis in A. chevrieri.

Adaptive characters of energy metabolism, thermogenesis and body mass in Eothenomys miletus during cold exposure and rewarming

2012 ◽  
Vol 62 (3) ◽  
pp. 263-276 ◽  
Author(s):  
Zhu Wan-long ◽  
Cai Jin-hong ◽  
Lian Xiao ◽  
Wang Zheng-kun
Keyword(s):  
Ambient Temperature ◽  
Energy Intake ◽  
Body Mass ◽  
Uncoupling Protein ◽  
Resting Metabolic Rate ◽  
Serum Leptin ◽  
Body Mass Regulation ◽  
Brown Adipose ◽  
And Behavior ◽  
Eothenomys Miletus

Environmental cues play important roles in the regulation of an animal’s physiology and behavior. The purpose of the present study was to test the hypothesis that ambient temperature was a cue to induce adjustments in body mass, energy intake and thermogenic capacity, associated with changes in serum leptin levels inEothenomys miletus. We found thatE. miletusincreased resting metabolic rate (RMR) and energy intake and decreased body mass when exposed to cold while it showed a significant increase in body mass after rewarming. The increase in body mass after rewarming was associated with the higher energy intake compared with the control. Uncoupling protein 1 (UCP1) content in brown adipose tissue (BAT) increased in the cold and reversed after rewarming. Serum leptin levels decreased in the cold while increased after rewarming, associated with the opposite changes in energy intake. Further, serum leptin levels were positively correlated with body mass and body fat mass. Together, these data supported our hypothesis that ambient temperature was a cue to induce changes in body mass and metabolism. Serum leptin, as a starvation signal in the cold and satiety signal in rewarming, was involved in the processes of thermogenesis and body mass regulation inE. miletus.

A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast

2001 ◽  
Vol 356 (3) ◽  
pp. 779-789 ◽  
Author(s):  
Jeff A. STUART ◽  
James A. HARPER ◽  
Kevin M. BRINDLE ◽  
Mika B. JEKABSONS ◽  
Martin D. BRAND
Keyword(s):  
Uncoupling Protein ◽  
Mitochondrial Protein ◽  
Physiological Property ◽  
Growth Defect ◽  
Yeast Mitochondria ◽  
Proton Conductance ◽  
Uncoupling Protein 1 ◽  
Mitochondrial Uncoupling ◽  
Mitochondrial Carrier ◽  
Brown Adipose

Uncoupling protein 1 (UCP1) from mouse was expressed in yeast and the specific (GDP-inhibitable) and artifactual (GDP-insensitive) effects on mitochondrial uncoupling were assessed. UCP1 provides a GDP-inhibitable model system to help interpret the uncoupling effects of high expression in yeast of other members of the mitochondrial carrier protein family, such as the UCP1homologues UCP2 and UCP3. Yeast expressing UCP1 at modest levels (approx. 1μg/mg of mitochondrial protein) showed no growth defect, normal rates of chemically uncoupled respiration and an increased non-phosphorylating proton conductance that was completely GDP-sensitive. The catalytic-centre activity of UCP1 in these yeast mitochondria was similar to that in mammalian brown-adipose-tissue mitochondria. However, yeast expressing UCP1 at higher levels (approx. 11μg/mg of mitochondrial protein) showed a growth defect. Their mitochondria had depressed chemically uncoupled respiration rates and an increased proton conductance that was partly GDP-insensitive. Thus, although UCP1 shows native behaviour at modest levels of expression in yeast, higher levels (or rates) of expression can lead to an uncoupling that is not a physiological property of the native protein and is therefore artifactual. This observation might be important in the interpretation of results from experiments in which the functions of UCP1homologues are verified by their ability to uncouple yeast mitochondria.

Effects of intravenous isoproterenol (β-agonist) administration on expression and synthesis of ovine uncoupling protein-1

1999 ◽  
Vol 1999 ◽  
pp. 164-164
Author(s):  
D.S. Finn ◽  
P. Trayhurn ◽  
J. Struthers ◽  
M.A. Lomax
Keyword(s):  
Adipose Tissue ◽  
Cold Stress ◽  
Uncoupling Protein ◽  
Uncoupling Protein 1 ◽  
Mitochondrial Uncoupling ◽  
Mitochondrial Uncoupling Protein ◽  
Neonatal Life ◽  
Adrenoceptor Agonist ◽  
Brown Adipose

A crucial factor in the prevention of hypothermia in the neonatal lamb is the functional activitation of a mitochondrial uncoupling protein (UCP1) in brown adipose tissue. UCP1 disappears from lamb brown fat over the first 14 days of life (Finn et al., 1998), but it is not known whether this process can be modulated in lambs by the release of catecholamines which have been established in rodents as a mediator of the response to cold stress. This study examines the effect of administering a β-adrenoceptor agonist on the disappearance of UCP1 and UCP1 mRNA during early neonatal life, using immunohistochemistry and in situ hybridization.

Nicotine infusion alters leptin and uncoupling protein 1 mRNA expression in adipose tissues of rats

2001 ◽  
Vol 280 (6) ◽  
pp. E867-E876 ◽  
Author(s):  
Keiko Arai ◽  
Kyongsong Kim ◽  
Katsumi Kaneko ◽  
Mitsue Iketani ◽  
Asuka Otagiri ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Mrna Expression ◽  
Wistar Rats ◽  
Uncoupling Protein ◽  
Plasma Concentrations ◽  
Uncoupling Protein 1 ◽  
Adipose Tissues ◽  
Brown Adipose ◽  
Male Wistar Rats ◽  
Infusion Period

We attempted to clarify whether leptin and uncoupling protein 1 (UCP1) are involved in the action of nicotine on the energy balance. Male Wistar rats were infused subcutaneously with nicotine (12 mg · kg−1 · day−1) for 4 or 14 days. At the end of the 4-day period, the plasma concentrations of leptin of the nicotine-treated and pair-fed rats were lower than those of the freely fed rats, although the levels of leptin mRNA expression in various white adipose tissues did not differ among the three groups. At the end of the 14-day nicotine infusion period, plasma concentrations of leptin were higher, and leptin mRNA expression in the omentum and epididymal and retroperitoneal adipose tissues was stronger in the nicotine-treated rats than in the pair-fed and freely fed rats. UCP1 mRNA expression in the brown adipose tissue of nicotine-treated was stronger than that of the pair-fed rats. These results suggest that continuous nicotine infusion differentially affects the synthesis and secretion of leptin according to the duration of infusion and stimulates UCP1 mRNA expression, probably in a manner independent of leptin.

Plasticity in food intake, thermogenesis and body mass in the tree shrew (Tupaia belangeri) is affected by food restriction and refeeding

2016 ◽  
Vol 66 (2) ◽  
pp. 201-217 ◽  
Author(s):  
Wen-rong Gao ◽  
Wan-long Zhu ◽  
Fang-yan Ye ◽  
Mu-lin Zuo ◽  
Zheng-kun Wang
Keyword(s):  
Adipose Tissue ◽  
Food Intake ◽  
Brown Adipose Tissue ◽  
Body Mass ◽  
Food Restriction ◽  
Uncoupling Protein ◽  
Tissue Mass ◽  
Serum Leptin ◽  
Brown Adipose ◽  
Ad Libitum

Physiological adjustments are important strategies for small mammals in response to variation in food availability. To determine the physiological mechanisms affected by food restriction and refeeding, tree shrews were restricted to 85% of initial food intake for 4 weeks and refedad libitumfor another 4 weeks. Changes in food intake, body mass, thermogenesis, body composition, mitochondrial cytochromecoxidase activity, uncoupling protein-1 content in brown adipose tissue and serum leptin levels were measured. The results showed that body mass, body fat mass and serum leptin levels significantly decreased in food restricted tree shrews, and increased when the restriction ended, showing a short “compensatory growth” rather than over-weight or obesity compared withad libitumcontrols. Resting metabolic rate, non-shivering thermogenesis, brown adipose tissue mass (mg), and uncoupling protein-1 content decreased significantly in response to food restriction, and returned to the control levels after the animals were refedad libitum, while the brown adipose tissue mass (%) and cytochromecoxidase activity remained stable during food restriction and refeeding. Food intake increased shortly after refeeding, which perhaps contributed to the rapid regaining of body mass. These results suggest thatTupaia belangerican adjust the status of its physiology integratively to cope with the lack of food by means of decreasing body mass, thermogenesis and serum leptin levels. Leptin may act as a starvation signal to predominantly mediate the reduction in body mass and energy expenditure.

scholarly journals Cold acclimation enhances UCP1 content, lipolysis, and triacylglycerol resynthesis, but not mitochondrial uncoupling and fat oxidation, in rat white adipocytes

2019 ◽  
Vol 316 (3) ◽  
pp. C365-C376 ◽  
Author(s):  
Diane M. Sepa-Kishi ◽  
Shailee Jani ◽  
Daniel Da Eira ◽  
Rolando B. Ceddia
Keyword(s):  
Fatty Acids ◽  
Cold Acclimation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Uncoupling Protein ◽  
Fat Oxidation ◽  
Uncoupling Protein 1 ◽  
Mitochondrial Uncoupling ◽  
Palmitate Oxidation ◽  
White Adipocytes ◽  
Brown Adipose

The objective of this study was to investigate whether cold-induced browning of the subcutaneous (Sc) inguinal (Ing) white adipose tissue (WAT) increases the capacity of this tissue to oxidize fatty acids through uncoupling protein 1 (UCP1)-mediated thermogenesis. To accomplish that, rats were acclimated to cold (4°C for 7 days). Subsequently, interscapular and aortic brown adipose tissues (iBAT and aBAT, respectively), epididymal (Epid), and Sc Ing WAT were used for adipocyte isolation. In BAT adipocytes, cold acclimation increased UCP1 content and palmitate oxidation either in the absence or presence of oligomycin, whereas in Sc Ing adipocytes glucose and palmitate oxidation were not affected, although multilocular adipocytes were formed and UCP1 content increased upon cold acclimation in the WAT. Furthermore, isoproterenol-stimulated cold Sc Ing adipocytes exhibited significantly lower rates of palmitate oxidation than control cells when exposed to oligomycin. These findings provide evidence that, despite increasing UCP1 levels, cold acclimation essentially reduced mitochondrial uncoupling-mediated fat oxidation in Sc Ing adipocytes. Conversely, glycerol kinase and phosphoenolpyruvate carboxykinase levels, isoproterenol-induced lipolysis, as well as glycerol and palmitate incorporation into lipids significantly increased in these cells. Therefore, instead of UCP1-mediated mitochondrial uncoupling, cold acclimation increased the capacity of Sc Ing adipocytes to export fatty acids and enhanced key components of the triacylglycerol resynthesis pathway in the Sc Ing WAT.

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