Plant growth promoting potential of multifarious endophytic Pseudomonas lini strain isolated from Cicer arietinum L.

2021 ◽  
pp. 1-11
Author(s):  
Rajat Maheshwari ◽  
Namita Bhutani ◽  
Pradeep Kumar ◽  
Pooja Suneja
Keyword(s):  
Plant Growth ◽  
Organic Acid ◽  
Cicer Arietinum ◽  
Endophytic Bacteria ◽  
Growth Parameters ◽  
Biotic Stresses ◽  
Iaa Production ◽  
Rdna Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Chickpea (Cicer arietinum), one of the major pulse crops in India, endured extreme reduction in production due to various abiotic and biotic stresses. Endophytic bacteria residing in the nodules and roots of chickpea plants enable host in combating these stresses. Twenty one endophytic bacteria isolated from nodules and roots of chickpea were screened for multiple plant growth promoting traits like ammonia, organic acid, siderophore, hydrogen cyanide (HCN) and phytohormone indole acetic acid (IAA) production. Out of these, 86% isolates produced ammonia, around 50% isolates produced organic acid, HCN and siderophore, 29% isolates produced ACC (1-aminocyclopropane-1-carboxylic acid) deaminase, while only 14% isolates solubilized phosphate. Interestingly, all the isolates were able to produce IAA ranging from 11.6–85.2 μg/ml, isolate CPJN 13 being the maximum IAA producer (85.5±2.33 μg/ml). Isolate CPJN13 was selected for IAA optimization studies. The yield of IAA increased up to 4 fold i.e. 331±4.96 μg/ml at optimized conditions. IAA production was also confirmed by TLC and HPLC analysis of crude IAA extract. The application of CPJN13 on chickpea seedlings resulted in significant increase in plant growth parameters. The 16S rDNA sequencing of CPJN13 revealed its similarity with Pseudomonas lini strain and submitted to NCBI with accession number MF574502. To best of our knowledge, this is the first report of the presence of P. lini as endophyte in chickpea nodules. The results of this study imply that the endophytic P. lini has a potential role to enhance the plant growth.

Influence of Plant Growth Promoting Rhizobium on Growth of Pigeon Pea [Cajanus cajan (L.) Millsp.] 

2021 ◽  
Author(s):  
H.A. Harshitha ◽  
Geeta Goudar
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Functional Characterization ◽  
Pigeon Pea ◽  
Iaa Production ◽  
Northern District ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Biological Nitrogen

Background: Symbiotic nitrogen fixation plays a major role in the production of million tons of total biological nitrogen. The Rhizobium not only fixes the nitrogen but also produces plant growth promoting hormones. In this view, the present study was undertaken to characterize the native Rhizobium isolates for their functional properties and evaluate on pigeon pea for their plant growth promotional activities. Methods: The present investigation was carried out during the year 2018-19 at UAS, Dharwad. Five of the native isolates obtained from Northern district of Karnataka were subjected for functional characterization using standard methods. The isolates were also tested for their plant growth promotional activities.Result: The IAA production in the isolates was ranged from 20.50 to 22.85 mg IAA/ml of broth and GA production from 13.40 to 14.51 mg/25ml of broth. ACC deamination activity in the isolates was ranged from 57.50 to 75.5 nmoles of α-ketobutyrate/mg/h of broth. All the isolates exhibited their ability to solubilize P and Zn. Out of ten isolates, four isolates exhibited potential to inhibit mycelial growth of Fusarium oxysporum f. sp. udum and per cent inhibition was ranged from 48.75 to 73.75. All the isolates were found to be positive for siderophore and HCN production. The isolate AMVPR98 performed better with respect to all the growth parameters of pigeon pea. Molecular characterization of isolate AMVPR98 revealed 99% similarity with Rhizobium pusense strain AB3.

scholarly journals Oak (Quercus robur) Associated Endophytic Paenibacillus sp. Promotes Poplar (Populus spp.) Root Growth In Vitro

2021 ◽  
Vol 9 (6) ◽  
pp. 1151
Author(s):  
Dorotėja Vaitiekūnaitė ◽  
Sigutė Kuusienė ◽  
Emilija Beniušytė
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Quercus Robur ◽  
Chemical Fertilizers ◽  
Iaa Production ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Populus Spp

Soil fertilization is necessary for high-demand crop production in agriculture and forestry. Our current dependence on chemical fertilizers has significant harmful side effects. Biofertilization using microorganisms is a sustainable way to limit the need for chemical fertilizers in various enterprises. Most plant endophytic bacteria have thus far been unstudied for their plant growth promoting potential and hence present a novel niche for new biofertilizer strains. We isolated English oak (Quercus robur) endophytic bacteria and tested them for plant growth promoting traits (PGPTs) such as nitrogen fixation, phosphate mineralization/solubilization, siderophore and indole-3-acetic acid (IAA) production. We also investigated the effect the selected isolate had on poplar (Populus spp.) microshoot vegetative growth parameters in vitro. In total 48 bacterial strains were isolated, attributed to Bacillus, Delftia, Paenibacillus, Pantoea and Pseudomonas genera. All the isolates displayed at least three PGPTs, with 39.6% of the isolates displaying all five (all were Pseudomonas spp.) and 18.75% displaying four. Based on relative abundance, Paenibacillus sp. isolate was selected for the poplar microshoot inoculation study. The isolate had a significant positive effect on poplar microshoot root growth and development. Two tested poplar genotypes both had increased lateral root number and density, fresh and dry root biomass. Furthermore, one genotype had increased length and number of adventitious roots as well as a decrease in fresh aboveground biomass. The root enhancement was attributed to IAA production. We propose this isolate for further studies as a potential biofertilizer.

scholarly journals Isolation, Identification, biocontrol activity and plant growth promoting of a superior strain Streptomyces tricolor strain HM10

2021 ◽  
Author(s):  
Medhat Rehan ◽  
Abdullah Alsohim ◽  
Hossam Abidou
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilization ◽  
Biologically Active ◽  
Iaa Production ◽  
Local Soil ◽  
Rdna Sequencing ◽  
Biocontrol Activity ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Abstract Purpose: Streptomyces are familiar with biocontrol activity and producing a broad range of biologically active substances. Our goal was to isolate a local Streptomyces bacteria that can promote plant growth via siderophore, indole acetic acid (IAA) production and phosphate solubilization and biocontrol the phytopathogens to reduce the heavy usage of chemical fertilizers and fungicides.Methods: The designated strains were isolated from local soil samples in Saudi Arabia via the standard serial dilution method, identified morphologically, by scanning electron microscope (SEM) and 16S rDNA sequencing and screened against many soils borne fungi. Siderophore, IAA production and phosphate solubilization were detected and measured.Results: A strain designated Streptomyces tricolor strain HM10 was isolated and showed aerial hyphae in SEM. Selected isolate showed highly homolog revealed to 99.69 % with the type strain Streptomyces tricolor LMG 20328. In vitro experiments showed a growth inhibition of ten phytopathogenes by strain HM10 fermentation broth and production of plant growth promoting compounds such as siderophore, IAA and solubilize phosphate. S. tricolor strain HM10 exhibited high antagonism effect against tested fungi (i.e. Colletotrichum gloeosporides with inhibition zone exceed 18 mm whereas the lowest effect was against Alternaria solani (8 mm)). Furthermore, the highest siderophore production was recorded to strain HM8 followed by strain HM10 with 64 and 22.56 h/c (halo zone area/colony area). With regard to IAA production, Streptomyces strain HM10 was the highest producer with value 273.02 µg/ml.Conclusion: Streptomyces tricolor HM10 is an important biological agent that can be used to promote plant growth and control of phytopathogen.

scholarly journals Evaluation of Phytase Producing Bacteria for Their Plant Growth Promoting Activities

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Prashant Singh ◽  
Vinod Kumar ◽  
Sanjeev Agrawal
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Growth Effect ◽  
Bacterial Isolates ◽  
Iaa Production ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacterial inoculants are known to possess plant growth promoting abilities and have potential as liquid biofertilizer application. Four phytase producing bacterial isolates (phytase activity in the range of 0.076–0.174 U/mL), identified asAdvenellaspecies (PB-05, PB-06, and PB-10) andCellulosimicrobiumsp. PB-09, were analyzed for their plant growth promoting activities like siderophore production, IAA production, HCN production, ammonia production, phosphate solubilization, and antifungal activity. All isolates were positive for the above characteristics except for HCN production. The solubilization index for phosphorus on Pikovskaya agar plates was in the range of 2–4. Significant amount of IAA (7.19 to 35.03 μg/mL) production and solubilized phosphate (189.53 to 746.84 μg/mL) was noticed by these isolates at different time intervals. Besides that, a greenhouse study was also conducted with Indian mustard to evaluate the potential of these isolates to promote plant growth. Effect of seed bacterization on various plant growth parameters and P uptake by plant were used as indicators. The plant growth promoting ability of bacterial isolates in pot experiments was correlated to IAA production, phosphate solubilization, and otherin vitrotests. On the basis of present findings, isolate PB-06 was most promising in plant growth promotion with multiple growth promoting characteristics.

scholarly journals Screening of Rice Endophytic Biofertilizers with Fungicide Tolerance and Plant Growth-Promoting Characteristics

2019 ◽  
Vol 11 (4) ◽  
pp. 1133 ◽  
Author(s):  
Fo-Ting Shen ◽  
Jui-Hung Yen ◽  
Chien-Sen Liao ◽  
Wen-Ching Chen ◽  
Yi-Ting Chao
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Agricultural Practice ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Bacillus Aryabhattai ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

The application of pesticides is unavoidable in conventional agricultural practice. To develop effective biofertilizers, bacterial strains with both pesticide tolerance and plant growth-promoting (PGP) traits were isolated for further testing. Seedlings of rice (Oryza sativa) were planted in soil with 1, 5, or 10 times the recommended rates of the fungicides etridiazole, metalaxyl, and tricyclazole. Endophytic bacteria were isolated from roots of rice seedlings. The bacterial 16S rDNA sequences and related PGP characteristics including potential nitrogen fixation, phosphorus-solubilizing and indole acetic acid (IAA) production ability were further examined. In all, 17 different strains were obtained from rice seedling roots; five strains with both nitrogen fixation potential and IAA production ability included Rhizobium larrymoorei E2, Bacillus aryabhattai E7, Bacillus aryabhattai MN1, Pseudomonas granadensis T6, and Bacillus fortis T9. Except for T9, all strains could tolerate two or more fungicides. We inoculated rice roots with the endophytic bacteria and all conferred rice growth-promoting ability. Bacillus aryabhattai MN1 was further tested and showed high tryptophan dose-dependent IAA production ability, tolerance towards etridiazole and metalaxyl application and should be considered a potential bacterial biofertilizer.

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