scholarly journals Evaluation of Phytase Producing Bacteria for Their Plant Growth Promoting Activities

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Prashant Singh ◽  
Vinod Kumar ◽  
Sanjeev Agrawal
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Growth Effect ◽  
Bacterial Isolates ◽  
Iaa Production ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacterial inoculants are known to possess plant growth promoting abilities and have potential as liquid biofertilizer application. Four phytase producing bacterial isolates (phytase activity in the range of 0.076–0.174 U/mL), identified asAdvenellaspecies (PB-05, PB-06, and PB-10) andCellulosimicrobiumsp. PB-09, were analyzed for their plant growth promoting activities like siderophore production, IAA production, HCN production, ammonia production, phosphate solubilization, and antifungal activity. All isolates were positive for the above characteristics except for HCN production. The solubilization index for phosphorus on Pikovskaya agar plates was in the range of 2–4. Significant amount of IAA (7.19 to 35.03 μg/mL) production and solubilized phosphate (189.53 to 746.84 μg/mL) was noticed by these isolates at different time intervals. Besides that, a greenhouse study was also conducted with Indian mustard to evaluate the potential of these isolates to promote plant growth. Effect of seed bacterization on various plant growth parameters and P uptake by plant were used as indicators. The plant growth promoting ability of bacterial isolates in pot experiments was correlated to IAA production, phosphate solubilization, and otherin vitrotests. On the basis of present findings, isolate PB-06 was most promising in plant growth promotion with multiple growth promoting characteristics.

Plant growth promoting traits shown by bacteria Brevibacterium frigrotolerans SMA23 Isolated from Aloe vera rhizosphere

2017 ◽  
Vol 37 (03) ◽  
Author(s):  
Meena . ◽  
Nayan Tara ◽  
Baljeet Singh Saharan
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Aloe Vera ◽  
Maximum Growth ◽  
Iaa Production ◽  
Rrna Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Brevibacterium frigrotolerans SMA23 is a gram positive rod shaped bacteria isolated from Aloe vera rhizosphere. 16S rRNA sequencing confirmed the identity of the bacterium as Brevibacterium frigrotolerans. It was capable of growing at temperatures ranging from 10°C to 35°C, but maximum growth was observed at 30°C. It is endowed with multiple plant growth promotion attributes such as phosphate solubilization, IAA production and siderophore production, which are expressed differentially at sub-optimal temperatures. At 10°C it was found to exhibit all the plant growth promotion attributes. This bacterial isolate was able to positively influence and promote the growth and nutrient uptake parameters of wheat (HD 2967) under glasshouse conditions.

scholarly journals Screening for Potential Plant Growth-Promoting Rhizobacteria (PGPR) Associated with Five Musa spp. Cultivars in SOCCSKSARGEN, Philippines

2018 ◽  
Vol 1 ◽  
pp. 5
Author(s):  
Giuseppe Haydn Lacorte ◽  
Remedios S. Flamiano ◽  
Malona V. Alinsug ◽  
Paolo M. Tagaloguin
Keyword(s):  
Microbial Population ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacterial Isolates ◽  
Iaa Production ◽  
Phosphate Solubilizers ◽  
Rhizospheric Bacteria ◽  
Nitrogen Fixers ◽  
Plant Growth Promoting ◽  
Growth Promoting

Rhizospheric bacteria (PGPR) associated with 5 banana cultivars planted in SOCCSKSARGEN were studied. Microbial population in newly established and old plantation was compared. Bacteria were purified and their beneficial characteristics were determined. This study was able to collect one hundred twenty (120) bacterial isolates, 20 (17%) of which were nitrogen-fixers, nineteen (19/20) were phosphate solubilizers and seventeen (17/20) were IAA producers. From this study, five bacterial isolates coded PE05, RS10, PE11, PE13, and PE18 were found positive for nitrogen fixation, phosphate solubilization and IAA production which may be further evaluated for their potential application in the formulation of biofertilizers for banana cultivation. Read full article here.

scholarly journals Diversity, biocontrol, and growth promotion potential of culturable endophytic fungi isolated from root, shoot, and leaf of wild Phoebe bournei (Hemsl.) Yang across the seasons

2021 ◽  
Author(s):  
Cun Yu ◽  
Ying Yao
Keyword(s):  
Plant Growth ◽  
Endophytic Fungi ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Leaf Spot Disease ◽  
Plant Growth Promoting Activities ◽  
Potential Applicability ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phoebe Bournei

Endophytic fungi were isolated from Phoebe bournei and their diversity and antimicrobial and plant growth-promoting activities were investigated. Of the 389 isolated endophytic fungi, 88.90% belonged to phylum Ascomycota and 11.10% to phylum Basidiomycota. The isolates were grouped into four taxonomic classes, 11 orders, 30 genera, and 45 species based on internal transcribed spacer sequencing and morphologic analysis. The host showed a strong affinity for the genera Diaporthe and Phyllosticta. The diversity of the fungi was highest in autumn, followed by spring and summer, and was lowest in winter. The fungi exhibited notable tissue specificity in P. bournei, and the species richness and diversity were highest in the root across all seasons. Five isolates showed antimicrobial activity against eight plant pathogens, and reduced the incidence of leaf spot disease in P. bournei. Additionally, 9 biocontrol isolates showed plant growth-promoting activity, with five significantly promoting P. bournei seedling growth. This is the first report on the endophytic fungi of P. bournei and their potential applicability to plant disease control and growth promotion.

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

scholarly journals Mechanism of phosphate solubilization and antifungal activity of Streptomyces spp. isolated from wheat roots and rhizosphere and their application in improving plant growth

Microbiology ◽  
2014 ◽  
Vol 160 (4) ◽  
pp. 778-788 ◽  
Author(s):  
Rahul Jog ◽  
Maharshi Pandya ◽  
G. Nareshkumar ◽  
Shalini Rajkumar
Keyword(s):  
Gene Expression ◽  
Antifungal Activity ◽  
Plant Growth ◽  
Potential Field ◽  
Phosphate Solubilization ◽  
Isocitrate Lyase ◽  
Malate Synthase ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

The application of plant-growth-promoting rhizobacteria (PGPR) at field scale has been hindered by an inadequate understanding of the mechanisms that enhance plant growth, rhizosphere incompetence and the inability of bacterial strains to thrive in different soil types and environmental conditions. Actinobacteria with their sporulation, nutrient cycling, root colonization, bio-control and other plant-growth-promoting activities could be potential field bio-inoculants. We report the isolation of five rhizospheric and two root endophytic actinobacteria from Triticum aestivum (wheat) plants. The cultures exhibited plant-growth-promoting activities, namely phosphate solubilization (1916 mg l−1), phytase (0.68 U ml−1), chitinase (6.2 U ml−1), indole-3-acetic acid (136.5 mg l−1) and siderophore (47.4 mg l−1) production, as well as utilizing all the rhizospheric sugars under test. Malate (50–55 mmol l−1) was estimated in the culture supernatant of the highest phosphate solublizer, Streptomyces mhcr0816. The mechanism of malate overproduction was studied by gene expression and assays of key glyoxalate cycle enzymes – isocitrate dehydrogenase (IDH), isocitrate lyase (ICL) and malate synthase (MS). The significant increase in gene expression (ICL fourfold, MS sixfold) and enzyme activity (ICL fourfold, MS tenfold) of ICL and MS during stationary phase resulted in malate production as indicated by lowered pH (2.9) and HPLC analysis (retention time 13.1 min). Similarly, the secondary metabolites for chitinase-independent biocontrol activity of Streptomyces mhcr0817, as identified by GC-MS and 1H-NMR spectra, were isoforms of pyrrole derivatives. The inoculation of actinobacterial isolate mhce0811 in T. aestivum (wheat) significantly improved plant growth, biomass (33 %) and mineral (Fe, Mn, P) content in non-axenic conditions. Thus the actinobacterial isolates reported here were efficient PGPR possessing significant antifungal activity and may have potential field applications.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria of wild legumes growing on sandy soils of Binh Thuan province, Vietnam

2021 ◽  
Vol 10 (3) ◽  
pp. 246-254
Author(s):  
Dang Thi Ngoc Thanh ◽  
Pham Thi Thu Ly ◽  
Pham Thi Nga ◽  
Pham Van Ngot
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Sandy Soils ◽  
Legume Species ◽  
Bacterial Isolates ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

The roots of two legume species (Tephrosia purpurea and Tephrosia villosa) that grew wild on dry sandy soils of Binh Thuan province were sources for isolating plant growth-promoting endophytic bacteria. Semi-solid LGI medium was used for the isolation of nitrogen-fixing bacteria from root extracts. All bacterial isolates isolates were evaluated for their ability to solubilize calcium orthophosphate on solid NBRIP medium and their ability to produce IAA in Burk's liquid medium supplemented with 100 mg/L tryptophan. The possibilities of nitrogen fixation, phosphate solubilization and IAA synthesis were all quantitative examined by colorimetric method. Twenty-two bacterial isolates of T. purpurea and 18 isolates of T. villosa were capable of nitrogen fixation in the range of 1.94 to 2.81 mg/L NH4+, whereas only 18 isolates of T. purpurea and 16 isolates of T. villosa showed phosphate solubilization in the range of 12.30 – 48.90 mg/L P2O5, and IAA production in the range of 0.38 – 12.72 mg/L. Sixteen outstanding bacterial isolates of the two legume species were identified by MALDI-TOF technique. The results showed that 13 isolates had high similarity with five bacterial genera including Klebsiella, Cronobacter, Enterobacter, Burkholderia, and Bacillus with score values in the range of 2.070 – 2.411.

scholarly journals Effects of Talaromyces purpureogenus on Cucumber Growth Promotion and Its Mechanism

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Zhao L ◽  
◽  
Zhao W ◽  
Deng H ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Scientific Basis ◽  
Promoting Effect ◽  
Iaa Production ◽  
Significant Difference ◽  
Application Potential ◽  
Promote Plant Growth ◽  
Growth Promoting

Some fungi may promote plant growth by production of siderophores, Indole Acetic Acid (IAA) and phosphorus dissolving capability. In this study, eight fungi were isolated from the mushroom substrate, and their siderophores production, IAA production and phosphorus dissolving traits were determined. Although there was no significant difference in IAA production among the eight fungi, but the strain M13026-2 was a fungus with strong growth promoting traits compared with other seven fungi. In order to study the correlation between the growth promoting effect of cucumber pot culture and the above three traits, five fungi with different strength of traits were tested in pot. As a result, M13026- 2 which was identified as Talaromyces purpureogenus could significantly improve the growth parameters of cucumber seedlings, and could colonize in the rhizosphere soil and the tissue of cucumber stably. All the results suggested that the most relevant to their ability to promote plant growth is the trait of phosphorus dissolving, followed by siderophores production. The results of this study will provide scientific basis for the efficient selection and identification of a large number of fungi resources with the function of promoting plant growth, and reveal the good application potential of T. purpureogenus in agriculture fields.

scholarly journals Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

2021 ◽  
Vol 8 (sp1) ◽  
pp. 17-24
Author(s):  
Tanvir Kaur ◽  
Rubee Devi ◽  
Divjot Kour ◽  
Ashok Yadav ◽  
Ajar Nath Yadav
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sugar Content ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

scholarly journals Phenetic and Molecular Diversity of Nitrogen Fixating Plant Growth Promoting Azotobacter Isolated from Semiarid Regions of India

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Devendra Jain ◽  
Jyoti Sharma ◽  
Gunnjeet Kaur ◽  
Ali Asger Bhojiya ◽  
Surya Chauhan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Restriction Analysis ◽  
Qualitative And Quantitative ◽  
Rrna Sequencing ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Dna Restriction ◽  
Pgp Activities ◽  
Growth Promoting

In the present study, 24 Azotobacter strains were isolated from soils of different areas of southern Rajasthan and characterized at biochemical, functional, and molecular levels. The isolated Azotobacter strains were gram negative and cyst forming when viewed under the microscope. These strains were also screened for their plant growth promoting activities and the ability of these isolates to survive under abiotic stress conditions viz. salt, pH, temperature, and drought stress. All the isolates showed IAA, siderophore, HCN, and ammonia production, whereas seven Azotobacter strains showed phosphate solubilization. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed significant diversity among Azotobacter strains and the dendrogram obtained differentiated twenty-four of the strains into two major clusters at a similarity coefficient of 0.64. Qualitative and quantitative N2 fixation abilities of these strains were also detrained, and the amounts of acetylene reduced by Azotobacter strains were in the range of 1.31 to 846.56 nmol C2H4 mg protein−1 h−1. The strains showing high nitrogen fixation ability with multiple PGP activities were selected for further pot studies, and these Azotobacter strains significantly increased the various plant growth parameters of maize plantlets. Furthermore, the best Azotobacter isolates were subjected to 16S rRNA sequencing and confirmed their identities as Azotobacter sp. The indigenous Azotobacter strains with multiple PGP activities could be further used for commercial production.

Plant growth promoting potential of multifarious endophytic Pseudomonas lini strain isolated from Cicer arietinum L.

2021 ◽  
pp. 1-11
Author(s):  
Rajat Maheshwari ◽  
Namita Bhutani ◽  
Pradeep Kumar ◽  
Pooja Suneja
Keyword(s):  
Plant Growth ◽  
Organic Acid ◽  
Cicer Arietinum ◽  
Endophytic Bacteria ◽  
Growth Parameters ◽  
Biotic Stresses ◽  
Iaa Production ◽  
Rdna Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Chickpea (Cicer arietinum), one of the major pulse crops in India, endured extreme reduction in production due to various abiotic and biotic stresses. Endophytic bacteria residing in the nodules and roots of chickpea plants enable host in combating these stresses. Twenty one endophytic bacteria isolated from nodules and roots of chickpea were screened for multiple plant growth promoting traits like ammonia, organic acid, siderophore, hydrogen cyanide (HCN) and phytohormone indole acetic acid (IAA) production. Out of these, 86% isolates produced ammonia, around 50% isolates produced organic acid, HCN and siderophore, 29% isolates produced ACC (1-aminocyclopropane-1-carboxylic acid) deaminase, while only 14% isolates solubilized phosphate. Interestingly, all the isolates were able to produce IAA ranging from 11.6–85.2 μg/ml, isolate CPJN 13 being the maximum IAA producer (85.5±2.33 μg/ml). Isolate CPJN13 was selected for IAA optimization studies. The yield of IAA increased up to 4 fold i.e. 331±4.96 μg/ml at optimized conditions. IAA production was also confirmed by TLC and HPLC analysis of crude IAA extract. The application of CPJN13 on chickpea seedlings resulted in significant increase in plant growth parameters. The 16S rDNA sequencing of CPJN13 revealed its similarity with Pseudomonas lini strain and submitted to NCBI with accession number MF574502. To best of our knowledge, this is the first report of the presence of P. lini as endophyte in chickpea nodules. The results of this study imply that the endophytic P. lini has a potential role to enhance the plant growth.

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