technology international corporation report high resolution optical measurements for dna operation dice game field report july 16 1975 secret dtic

2020 ◽  
Author(s):  
Tyler N. Chen ◽  
Anushka Gupta ◽  
Mansi Zalavadia ◽  
Aaron M. Streets

AbstractSingle-cell RNA sequencing (scRNA-seq) enables the investigation of complex biological processes in multicellular organisms with high resolution. However, many phenotypic features that are critical to understanding the functional role of cells in a heterogeneous tissue or organ are not directly encoded in the genome and therefore cannot be profiled with scRNA-seq. Quantitative optical microscopy has long been a powerful approach for characterizing diverse cellular phenotypes including cell morphology, protein localization, and chemical composition. Combining scRNA-seq with optical imaging has the potential to provide comprehensive single-cell analysis, allowing for functional integration of gene expression profiling and cell-state characterization. However, it is difficult to track single cells through both measurements; therefore, coupling current scRNA-seq protocols with optical measurements remains a challenge. Here, we report Microfluidic Cell Barcoding and Sequencing (μCB-seq), a microfluidic platform that combines high-resolution imaging and sequencing of single cells. μCB-seq is enabled by a novel fabrication method that preloads primers with known barcode sequences inside addressable reaction chambers of a microfluidic device. In addition to enabling multi-modal single-cell analysis, μCB-seq improves gene detection sensitivity, providing a scalable and accurate method for information-rich characterization of single cells.


2015 ◽  
Vol 82 (7-8) ◽  
Author(s):  
Markus Schake ◽  
Markus Schulz ◽  
Peter Lehmann

AbstractThe determination of surface roughness is a common challenge in industrial quality assurance. Because tactile techniques like the stylus method or atomic force microscopy run the risk of damaging the measurement object there is a high demand for contact-free optical measurements. In this contribution we demonstrate the feasibility of a high resolution fiber-coupled interferometric point sensor with periodical path length modulation to determine the surface profile of rough surfaces. Measurements on two specimens characterized by different roughness parameters are presented and corrections for common measurement errors, due to phase ambiguity are discussed.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dae-Myeong Geum ◽  
SangHyeon Kim ◽  
Seong Kwang Kim ◽  
SooSeok Kang ◽  
JiHoon Kyhm ◽  
...  

AbstractIn this study, multicolor photodetectors (PDs) fabricated by using bulk p-i-n-based visible GaAs and near-infrared InGaAs structures were monolithically integrated through a high-throughput epitaxial lift-off (ELO) process. To perform multicolor detection in integrated structures, GaAs PDs were transferred onto InGaAs PDs by using a Y2O3 bonding layer to simultaneously detect visible and near-infrared photons and minimize the optical loss. As a result, it was found that the GaAs top PD and InGaAs bottom PD were vertically aligned without tilting in x-ray diffraction (XRD) measurement. A negligible change in the dark currents for each PD was observed in comparison with reference PDs through electrical characterization. Furthermore, through optical measurements and simulation, photoresponses were clearly revealed in the visible and near-infrared band for the material’s absorption region, respectively. Finally, we demonstrated the simultaneous multicolor detection of the visible and near-infrared region,which implies individual access to each PD without mutual interference. These results are a significant improvement for the fabrication of multicolor PDs that enables the formation of bulk-based multicolor PDs on a single substrate with a high pixel density and nearly perfect vertical alignment for high-resolution multicolor imaging.


2011 ◽  
Vol 29 (2) ◽  
pp. 299-319 ◽  
Author(s):  
T. Pitkänen ◽  
A. T. Aikio ◽  
O. Amm ◽  
K. Kauristie ◽  
H. Nilsson ◽  
...  

Abstract. We report observations of a sequence of quiet-time Earthward bursty bulk flows (BBFs) measured by the Cluster spacecraft in the near-tail plasma sheet (XGSM ~ −12 to −14 RE) in the evening sector, and by simultaneous high-resolution measurements in the northern conjugate ionosphere by the EISCAT radars, a MIRACLE all-sky camera and magnetometers, as well as a meridian-scanning photometer (MSP) in the Scandinavian sector on 17 October 2005. The BBFs at Cluster show signatures that are consistent with the plasma "bubble" model (Chen and Wolf, 1993, 1999), e.g. deflection and compression of the ambient plasma in front of the Earthward moving bubble, magnetic signatures of a flow shear region, and the proper flows inside the bubble. In addition, clear signatures of tailward return flows around the edges of the bubble can be identified. The duskside return flows are associated with significant decrease in plasma density, giving support to the recent suggestion by Walsh et al. (2009) of formation of a depleted wake. However, the same feature is not seen for the dawnside return flows, but rather an increase in density. In the ionosphere, EISCAT and optical measurements show that each of the studied BBFs is associated with an auroral streamer that starts from the vicinity of the polar cap boundary, intrudes equatorward, brakes at 68–70° aacgm MLAT and drifts westward along the proton oval. Within the streamer itself and poleward of it, the ionospheric plasma flow has an equatorward component, which is the ionospheric manifestation of the Earthward BBF channel. A sharp velocity shear appears at the equatorward edge of a streamer. We suggest that each BBF creates a local velocity shear in the ionosphere, in which the plasma flow poleward of and inside the streamer is in the direction of the streamer and southeastward. A northwestward return flow is located on the equatorward side. The return flow is associated with decreased plasma densities both in the ionosphere and in the magnetosphere as measured by EISCAT and Cluster, respectively. In summary, we present the first simultaneous high-resolution observations of BBF return flows both in the plasma sheet and in the ionosphere, and those are in accordance with the bubble model. The results apply for the duskside return flows, but the manifestation of dawnside return flows in the ionosphere requires further studies. Finally, EISCAT measurements indicate increased nightside reconnection rate during the ~35-min period of BBFs. We suggest that the observed temporal event of IMF rotation to a more southward direction produces enhanced open flux transport to the nightside magnetotail, and consequently, the nightside reconnection rate is increased.


Geosciences ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 170 ◽  
Author(s):  
Pappone ◽  
Aucelli ◽  
Mattei ◽  
Peluso ◽  
Stefanile ◽  
...  

In this paper, we present the results of a multidisciplinary study aimed to reconstruct the Roman coastal landscape between Pizzofalcone hill and Megaris islet—the area of the ancient Parthenope, the first settlement along the Naples coast. This coastal sector was surveyed by a team of specialized divers (archaeologists and geomorphologists) and by using an Unmanned Surface Vessel (USV) equipped with acoustic and optical sensors. The indirect surveys provided a high-resolution dataset of morpho-acoustic and optical measurements, useful to obtain the geological, geomorphological and archaeological interpretations necessary to formulate hypotheses on the functionality of the complex submerged archaeological structure detected in the study area. In particular, the integration between the surveyed data, the high-resolution seafloor mapping and the previous knowledge deriving from the 1980s underwater research carried out by Centro Studi Subacquei, led us to interpret the submerged remains as a vivarium related to a 1st century BC Roman villa. Finally, by measuring the submersion of several channels and a well-preserved crepido, a relative sea level during the period of use at –2.2 m ±0.2 m mean sea level (MSL) was deduced, in agreement with the previous geoarchaeological studies realized in the near coastal sectors.


The lines λ 444.2nm, λ 304.2nm and λ 306.5nm of the arc spectrum of platinum have been studied under high resolution by using digital recording interferometry. The work extends the range of nuclei for which isotope shifts have been measured to include 190 Pt, and has provided some improvement in accuracy for the shifts involving other isotopes. Data are now available for all the stable platinum nuclei; the relative shifts, which are closely proportional to the changes in mean square radius of the proton distribution, are as follows: 190, 192; 0.905 ± 0.028.192, 194; 0.969 ± 0.007. 194, 195; 0.456 ± 0.004. 194, 196; 1.0. 196, 198; 1.084 ± 0.004. The data are discussed in terms of recent measurements of nuclear deformation in platinum. The hyperfine splittings of some levels have also been deduced from the optical measurements.


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