The Autism Phenome Project: Toward Identifying Clinically Meaningful Subgroups of Autism

One of the most universally accepted facts about autism is that it is heterogenous. Individuals diagnosed with autism spectrum disorder have a wide range of behavioral presentations and a variety of co-occurring medical and mental health conditions. The identification of more homogenous subgroups is likely to lead to a better understanding of etiologies as well as more targeted interventions and treatments. In 2006, we initiated the UC Davis MIND Institute Autism Phenome Project (APP) with the overarching goal of identifying clinically meaningful subtypes of autism. This ongoing longitudinal multidisciplinary study now includes over 400 children and involves comprehensive medical, behavioral, and neuroimaging assessments from early childhood through adolescence (2–19 years of age). We have employed several strategies to identify sub-populations within autistic individuals: subgrouping by neural, biological, behavioral or clinical characteristics as well as by developmental trajectories. In this Mini Review, we summarize findings to date from the APP cohort and describe progress made toward identifying meaningful subgroups of autism.

Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles

Indoor air pollutants (IAP), which can pose a serious risk to human health, include biological pollutants, nitric oxide (NO), nitrogen dioxide (NO2), volatile organic compounds (VOC), sulfur dioxide (SO2), carbon monoxide (CO), carbon dioxide (CO2), silica, metals, radon, and particulate matter (PM). The aim of our work is to conduct a multidisciplinary study of fine silica particles (<2.5 μm) in the presence or absence of ozone (O3), and evaluate their potential cytotoxicity using MTS, micronucleus, and the comet test in two cell lines. We analyzed A549 (human basal alveolar epithelial cell adenocarcinoma) and Hs27 (human normal fibroblasts) exposed to dynamic conditions by an IRC simulator under ozone flow (120 ppb) and in the presence of silica particles (40 μg/h). The viability of A549 and Hs27 cells at 48 and 72 h of exposure to silica or silica/ozone decreases, except at 72 h in Hs27 treated with silica/ozone. The micronucleus and comet tests showed a significant increase in the number of micronuclei and the % of DNA in the queue, compared to the control, in both lines in all treatments, even if in different cell times/types. We found that silica alone or with more O3 causes more pronounced genotoxic effects in A549 tumor cells than in normal Hs27 fibroblasts.

Dengue virus infection modifies mosquito blood-feeding behavior to increase transmission to the host

Mosquito blood-feeding behavior is a key determinant of the epidemiology of dengue viruses (DENV), the most-prevalent mosquito-borne viruses. However, despite its importance, how DENV infection influences mosquito blood-feeding and, consequently, transmission remains unclear. Here, we developed a high-resolution, video-based assay to observe the blood-feeding behavior of Aedes aegypti mosquitoes on mice. We then applied multivariate analysis on the high-throughput, unbiased data generated from the assay to ordinate behavioral parameters into complex behaviors. We showed that DENV infection increases mosquito attraction to the host and hinders its biting efficiency, the latter resulting in the infected mosquitoes biting more to reach similar blood repletion as uninfected mosquitoes. To examine how increased biting influences DENV transmission to the host, we established an in vivo transmission model with immuno-competent mice and demonstrated that successive short probes result in multiple transmissions. Finally, to determine how DENV-induced alterations of host-seeking and biting behaviors influence dengue epidemiology, we integrated the behavioral data within a mathematical model. We calculated that the number of infected hosts per infected mosquito, as determined by the reproduction rate, tripled when mosquito behavior was influenced by DENV infection. Taken together, this multidisciplinary study details how DENV infection modulates mosquito blood-feeding behavior to increase vector capacity, proportionally aggravating DENV epidemiology. By elucidating the contribution of mosquito behavioral alterations on DENV transmission to the host, these results will inform epidemiological modeling to tailor improved interventions against dengue.

Multidisciplinary Study of Burnt Deposits at Surungur, Fergana Valley, Southern Kyrgyzstan

Burnt deposits are an important source of information on ancient lifestyles, providing the possibility of reconstructing the size, intensity of use, and functions of fi replaces at prehistoric settlements, and to assess fuel sources. We outline the results of a multidisciplinary study of fi replaces and their contexts at Surungur—a stratifi ed site in the Fergana Valley, in southern Kyrgyzstan. Sixteen samples from ash lenses and intermediate deposits were studied by rock-magnetism, gas chromatography mass spectrometry (GC-MS), and X-ray fl uorescence (XRF). The rock-magnetic analysis suggests that the origin of all samples from ash lenses was anthropogenic. Types of fuel were reconstructed. At the initial stage (Early Holocene), the encompassing deposits likely resulted from short-term occupation, and fuel consisted of wood and grass/ dung. In the Middle Holocene, occupation became more long-term, as evidenced by maximal heating temperatures and high concentration of fi replaces. During the Late Holocene, habitation intensity on the platform under the stone ledge remained the same, but heating was less intense. Wood and grass/dung were used as fuel at all stages, suggesting that wood was available in the region throughout the Holocene.

Multidisciplinary Study of Cores From the Wetland of El Melah, Southwestern Tunisia

Saline systems are candidates to be threatened by climatic change. In terms of methodology and materials, color identification, geochemistry, and mineralogy analysis were used. The spectral analysis of data of the amount of active K and Na firstly shows the individualization of a cycle stretching along with 1000 yr. Then, a less pronounced cycle of 2500 years is marked with a lower intensity. Also, on the spectral analysis of K data, the drowned cycles of 650 and 500 years are marked by weak intensities not reaching the threshold level. These two comparable cycles may be artifacts due to analysis errors or variability in the rate of sedimentation resulting in the bifurcation of two hybrid cycles from one real cycle. This work confirmed the cycles found by color studies through real analyses such as geochemical and magnetic measurements. As a matter of fact, the majority of cycles found out by the spectral analysis of colors data are confirmed through analyses.

‘It is not fashionable to suffer nowadays’: Community motivations to repeatedly participate in outreach HIV testing indicate UHC potential in Tanzania

Objective This study examined people’s motivations for (repeatedly) utilizing HIV testing services during community-based testing events in urban and rural Shinyanga, Tanzania and potential implications for Universal Health Coverage (UHC). Methods As part of a broader multidisciplinary study on the implementation of a HIV Test and Treat model in Shinyanga Region, Tanzania, this ethnographic study focused on community-based testing campaigns organised by the implementing partner. Between April 2018 and December 2019, we conducted structured observations (24), short questionnaires (42) and in-depth interviews with HIV-positive (23) and HIV-negative clients (8). Observations focused on motivations for (re-)testing, and the counselling and testing process. Thematic analysis based on inductive and deductive coding was completed using NVivo software. Results Regular HIV testing was encouraged by counsellors. Most participants in testing campaigns were HIV-negative; 51.1% had tested more than once over their lifetimes. Testing campaigns provided an accessible way to learn one’s HIV status. Motivations for repeat testing included: monitoring personal health to achieve (temporary) reassurance, having low levels of trust toward sexual partners, feeling at risk, seeking proof of (ill)-health, and acting responsibly. Repeat testers also associated testing with a desire to start treatment early to preserve a healthy-looking body, should they prove HIV positive. Conclusions Community-based testing campaigns serve three valuable functions related to HIV prevention and treatment: 1) enable community members to check their HIV status regularly as part of a personalized prevention strategy that reinforces responsible behaviour; 2) identify recently sero-converted clients who would not otherwise be targeted; and 3) engage community with general prevention and care messaging and services. This model could be expanded to include routine management of other (chronic) diseases and provide an entry for scaling up UHC.

Prevalence of Computer Vision Syndrome in School Going Children During COVID Era

Background: Prolonged use of electronic devices poses a significant risk of Computer vision syndrome (CVS). Computer Vision syndrome is emerging as the next unexpected epidemic of the COVID era. Almost all children attending regular online schools are suffering from mild to severe Computer vision syndrome. Besides health problems, CVS also results in concentrate difficulties in studies and decrease a child’s performance. Awareness regarding CVS is increasing among folks of all ages since it has hit not just school going children but their parents as well who are pursuing work from home. Materials and Methods: From March 2020 to March 2021, a multidisciplinary study was conducted to look at the increase in CVS and related factors among school-age children in School of Scholars, Nagpur taking online classes. The randomized controlled trial was used to select 600-school aged children between the ages of 8 and 17, and data was collected using a systematic questionnaire completed by the children's parents. Results: CVS was found in 345 (57.5%) of the 600 children studied, which is very concerning. With proportions of 83.50 percent, 66.33 percent, and 62.17 percent, respectively, the most common reported signs of CVS were eyestrain, eye inflammation, and eye pain. CVS are linked to daily screen use and pre-existing eye disorder. Conclusion: To deter CVS, it is critical to reduce screen time, improve parent and child understanding of safety precautions, and receive management support. To protect a child's overall well-being, we encourage policymakers and parents to restrict e-learning, since it will never be a suitable replacement for conventional classroom instruction.