New Insights from Locally Resolved Hydrodynamics in Stirred Cell Culture Reactors

The link between hydrodynamics and biological process behavior of antibody-producing mammalian cell cultures is still not fully understood. Common methods to describe dependencies refer mostly to averaged hydrodynamic parameters obtained for individual cultivation systems. In this study, cellular effects and locally resolved hydrodynamics were investigated for impellers with different spatial hydrodynamics. Therefore, the hydrodynamics, mainly flow velocity, shear rate and power input, in a single- and a three-impeller bioreactor setup were analyzed by means of CFD simulations, and cultivation experiments with antibody-producing Chinese hamster ovary (CHO) cells were performed at various agitation rates in both reactor setups. Within the three-impeller bioreactor setup, cells could be cultivated successfully at much higher agitation rates as in the single-impeller bioreactor, probably due to a more uniform flow pattern. It could be shown that this different behavior cannot be linked to parameters commonly used to describe shear effects on cells such as the mean energy dissipation rate or the Kolmogorov length scale, even if this concept is extended by locally resolved hydrodynamic parameters. Alternatively, the hydrodynamic heterogeneity was statistically quantified by means of variance coefficients of the hydrodynamic parameters fluid velocity, shear rate, and energy dissipation rate. The calculated variance coefficients of all hydrodynamic parameters were higher in the setup with three impellers than in the single impeller setup, which might explain the rather stable process behavior in multiple impeller systems due to the reduced hydrodynamic heterogeneity. Such comprehensive insights lead to a deeper understanding of the bioprocess.

The Influence of a Suspended Cage Aquaculture Farm on the Hydrodynamic Environment in a Semienclosed Bay, SE China

Hydrodynamic responses of the aquaculture farm structures have been increasingly studied because of their importance in informing the aquaculture carrying capacity and ecological sustainability. The hydrodynamical effect of the suspended cage farm on flow structures and vertical mixing in the Sansha Bay, SE China, is examined using observational data of two comparative stations inside and outside the cage farm. The results show that current velocities are relatively uniform in the vertical except a bottom boundary layer outside the cage farm. Within the cage farm, the surface boundary layer produced by the cage-induced friction is obvious with current velocities decreasing upward, combining the classic bottom boundary layer to form a “double-drag layers” structure in the water column. The cage-induced drag decreases with water depth in the surface boundary layer with a maximum thickness of 3/4 the water column, and the current velocities can be reduced by 54%. The cage-induced friction can also significantly hinder the horizontal water exchange in the farm. Periodic stratification phenomena exist at both stations under the influence of lateral circulation. However, the subsurface (5–10 m below the sea surface) water column below the cage facilities is well-mixed as indicated by the vertical density profile, where the velocity shear (10–3 m–2) is about 10 times higher than that of the subsurface layer outside the cage farm. Therefore, we speculate that the well-mixing of the subsurface water column results from the local turbulence induced by the velocity shear, which in turn is produced by the friction of cage structures.

High-Velocity Shear and Soft Friction at the Nanometer Scale

We study high-speed friction on soft polymer materials by measuring the amplitude dependence of cyclic lateral forces on the atomic force microscope (AFM) tip as it slides on the surface with fixed contact force. The resulting dynamic force quadrature curves separate the elastic and viscous contributions to the lateral force, revealing a transition from stick-slip to free-sliding motion as the velocity increases. We explain force quadratures and describe how they are measured, and we show results for a variety of soft materials. The results differ substantially from the measurements on hard materials, showing hysteresis in the force quadrature curves that we attribute to the finite relaxation time of viscoelastic surface deformation.

Evolution of Kelvin–Helmholtz Instability in the Fan-spine Topology

We use multiwavelength imaging observations from the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamics Observatory to study the evolution of the Kelvin–Helmholtz (K–H) instability in a fan-spine magnetic field configuration. This magnetic topology exists near an active region AR12297 and is rooted in a nearby sunspot. In this magnetic configuration, two layers of cool plasma flow in parallel and interact with each other inside an elongated spine. The slower plasma flow (5 km s−1) is the reflected stream along the spine’s field lines from the top, which interacts with the impulsive plasma upflows (114–144 km s−1) from below. This process generates a shear motion and subsequent evolution of the K–H instability. The amplitude and characteristic wavelength of the K–H unstable vortices increase, satisfying the criterion of the fastest-growing mode of this instability. We also describe how the velocity difference between two layers and the velocity of K–H unstable vortices are greater than the Alfvén speed in the second denser layer, which also satisfies the criterion of the growth of the K–H instability. In the presence of the magnetic field and sheared counterstreaming plasma as observed in the fan-spine topology, we estimate the parametric constant Λ ≥ 1, which confirms the dominance of velocity shear and the evolution of the linear phase of the K–H instability. This observation indicates that in the presence of complex magnetic field structuring and flows, the fan-spine configuration may evolve into rapid heating, while the connectivity changes due to the fragmentation via the K–H instability.

Effects of Spur Dyke’s Orientation on Bed Variation in Channel Bend

Spur dykes also known as Groynes are often used to either divert or attract the flow from the main structure to safeguard their life. Those structures may be bridge piers, abutments or any similar hydraulics structures. Spur dykes are also used to save the cutting of banks on concave side of stream. Lots of work have been done in recent past on spur dykes by many investigators in which various hydraulic and geometrical parameters of spur dykes such as discharge, sediment size, flow velocity, shear stress, spur dykes shape, size and submergence etc. are studied in detail. But mostly all the studies were pointed out in straight open channels. Very few studies were done in curved channel and only their similar effects were studied. In present thesis main emphasis is given to study the effect of orientation and location of spur dykes in meandering channel on the bed of downstream side. In the present study experimental work has been carried out in 80° bend and constant discharge (Q = 4.5 l/s) is allowed to pass in channel without spur dyke. It is found that maximum scouring occurs at angular displacement θ = 60° to 80° in the vicinity of outer bank. To minimize this scouring, spur dyke has been installed at angular displacement θ = 20°, 40° & 60° by changing the dyke angle α = 60°, 90° & 120° respectively. It is found that scouring at θ = 60° is reduced by installing spur dyke at angular displacement θ = 40° which is oriented at α = 60° and scouring at θ = 80° is reduced by installing spur dyke at angular displacement θ = 60° which is oriented at α = 60°.

STUDY OF OILSEED MATERIAL RHEOLOGY DURING EXTRUSION PROCESSING

Определены параметры течения пластичной жидкости при экструзионной переработке масличных культур. В качестве экспериментального материала использовали мезгу, выходящую из жаровни и поступающую в экструдер. Вязкость измеряли на ротационном вискозиметре при избыточном гидростатическом давлении 981 Па. Высота слоя материала в измеряемой ячейке составила 38 мм. Скорость сдвига в процессе измерения варьировали от 1 до 10 рад/с. Проведены эксперименты по определению зависимости вязкости от масличности материала, напряжения и скорости сдвига, а также гидравлического давления на исследуемый материал. Анализ результатов эксперимента свидетельствует о наличии структурообразования материала, поступающего в экструдер. Полученные параметры инженерной модели позволяют прогнозировать реологию вязкопластичного течения в широком диапазоне скоростей сдвига в каналах шнекового пресса. The parameters of the flow of plastic liquid during the extrusive processing of oilseed material are defined. The pulp coming from the brazier and into an extruder was used as experimental material. The viscosity was measured with rotational viscometer under excessive hydrostatic pressure of 981 PA. The height of the layer of material in the measuring cell was 38 mm. Shear rate during the measurement ranged from 1 to 10 rad/s. The experiments to determine the dependence of viscosity on oil content material, pressure, and velocity shear, and hydraulic pressure on the oilseed material were carrying out. Analysis of the results of the experiment indicates the presence of structure formation of the material coming into the extruder.

Bifurcated Current Sheet Observed on the Boundary of Kelvin-Helmholtz Vortices

On May 5, 2017 MMS observed a bifurcated current sheet at the boundary of Kelvin-Helmholtz vortices (KHVs) developed on the dawnside tailward magnetopause. We use the event to enhance our understanding of the formation and structure of asymmetric current sheets in the presence of density asymmetry, flow shear, and guide field, which have been rarely studied. The entire current layer comprises three separate current sheets, each corresponding to magnetosphere-side sunward separatrix region, central near-X-line region, and magnetosheath-side tailward separatrix region. Two off-center structures are identified as slow-mode discontinuities. All three current sheets have a thickness of ∼0.2 ion inertial length, demonstrating the sub-ion-scale current layer, where electrons mainly carry the current. We find that both the diamagnetic and electron anisotropy currents substantially support the bifurcated currents in the presence of density asymmetry and weak velocity shear. The combined effects of strong guide field, low density asymmetry, and weak flow shear appear to lead to asymmetries in the streamlines and the current-layer structure of the quadrupolar reconnection geometry. We also investigate intense electrostatics waves observed on the magnetosheath side of the KHV boundary. These waves may pre-heat a magnetosheath population that is to participate into the reconnection process, leading to two-step energization of the magnetosheath plasma entering into the magnetosphere via KHV-driven reconnection.

Spectral and correlation analysis of microturbulences in the spherical Globus-M/M2 tokamaks

Results of the studies on turbulences carried out on the Globus-M2 and Globus-M tokamaks are presented. The main focus was on the analysis of the data obtained using Doppler backscattering method (DBS). The developed codes for the analysis of DBS signals allowed to study the effects of turbulences on the operational mode of the tokamak. A description of the data processing codes is also included. The analysis performed indicates the suppression of turbulence and the formation of a velocity shear during the L-H transition. It was also successfully used to study density fluctuations during and between edge localized modes (ELMs). Spectral and correlation analysis also led to the discovery of limit-cycle oscillations (LCO) and quasi coherent fluctuations (QCFs) during the I-phase.