Studies on the achene morphology of Turkish species of Scorzonera L. (Asteraceae) using light and scanning electron microscopy

Macro- and micromorphological features of achenes belonging to 59 taxa from Turkey were observed via light and scanning electron microscopy. The findings agree with the traditional subdivision of Scorzonera into S. subg. Scorzonera, S. subg. Podospermum and S. subg. Pseudopodospermum. Members of S. subg. Podospermum were distinguished by achenes with a distinct carpopodium and horizontally striped epidermal cell surface; members of S. subg. Pseudopodospermum were distinguished by achenes with a conspicuous carpopodium and often ruminate and sometimes rugose-granulate or smooth epidermal surface, and members of Scorzonera s.str were distinguished by achenes without a carpopodium and with various combinations of surface patterns. The results also showed that the length, pubescence and surface pattern of achenes, as well as the carpopodium and anticlinal and periclinal walls of the epidermal cells are valuable for delimiting the examined species within the genus.

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Appearance of phenotypic plasticity of leaves in psammophyte Corynephorus canescens during flooding

Plant Introduction ◽

10.46341/pi2021011 ◽

2021 ◽

Vol 91-92 ◽

pp. 24-35

Author(s):

Olena Nedukha

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Phenotypic Plasticity ◽

Silicon Content ◽

Crystalline Silicon ◽

Oxygen Balance ◽

Epidermal Surface ◽

Corynephorus Canescens ◽

Periclinal Walls ◽

Scanning Electron

The results of the study of the leaf structure in psammophyte Corynephorus canescens, which grew under controlled conditions and flooding using the methods of light microscopy, scanning electron microscopy, and laser confocal microscopy, are presented. This study revealed common and distinctive signs of morphological and anatomical parameters of C. canescens leaves in the phase of vegetative growth. Among the common features were the shape and size of the leaf laminas, hypostomatic type of the leaf, isolateral structure of the parenchyma, the thick-walled epidermis, and the bilayered hypodermis. Among the distinctive features were the signs of the destruction of cells in the photosynthetic parenchyma, change in their shape with the formation of protuberances at the cells’ poles, and almost doubling area of the aerenchyma in C. canescens leaves under flooding conditions. Scanning electron microscopy showed the similarity of ultrastructure and density of trichomes on the adaxial surface, excepting the formation of cuticular wax structures on the epidermal surface of the leaves in flooded plants. The subcellular localization of silicon inclusions was studied for the first time. The presence of amorphous and small crystalline silicon inclusions in the periclinal walls of the main epidermal cells and amorphous silicon inclusions in leaf trichomes was established. An increase in the relative silicon content along the trichomes in the leaves’ epidermis after flooding was revealed. It was assumed that the phenotypic plasticity of C. canescens, is realized through the increasing area of aerenchyma in leaves and increasing silicon content in trichomes. Such plasticity helps to optimize both the oxygen balance of plants and water balance in flooded plants, thus increasing the species’ resistance to prolonged flooding.

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The Mesothelial Cell as a Non-Thrombogenic Surface

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661149 ◽

1984 ◽

Vol 52 (02) ◽

pp. 102-104 ◽

Cited By ~ 25

Author(s):

L J Nicholson ◽

J M F Clarke ◽

R M Pittilo ◽

S J Machin ◽

N Woolf

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Blood Flow ◽

Cell Surface ◽

Mesothelial Cell ◽

Mesothelial Cells ◽

Plastic Film ◽

In Vitro System ◽

Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

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Concanavalin a and wheat germ agglutinin receptors on dictyostelium: Their visualization by scanning electron microscopy with microspheres

The Journal of Cell Biology ◽

10.1083/jcb.71.1.314 ◽

1976 ◽

Vol 71 (1) ◽

pp. 314-322 ◽

Cited By ~ 28

Author(s):

R Molday ◽

R Jaffe ◽

D McMahon

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Cell Surface ◽

Wheat Germ ◽

Cellular Interactions ◽

Con A ◽

Stages Of Development ◽

Lectin Receptors ◽

Plant Lectins ◽

Scanning Electron

The cellular slime mold, Dictyostelium discoideum, is a convenient model for studying cellular interactions during development. Evidence that specific cell surface components are involved in cellular interactions during its development has been obtained by Gerisch and co-workers (1, 2) using immunological techniques. Smart and Hynes (3) have shown that a cell surface protein can be iodinated on cells in aggregation phase, but not in vegetative phase, by the lactoperoxidase procedure. Recently, McMahon et al. (4), and Hoffman and McMahon have demonstrated, by SDS gel electrophoresis, considerable differences in cell surface proteins and glycoproteins of plasma membranes isolated from cells at different stages of development. Plant lectins have also been used to monitor changes in cell surface properties of D. discoideum cells during development. Weeks and co-workers (5, 6) have detected differences in the binding and agglutination of cells by concanavalin A (Con A). Gillette and Filosa (7) have shown that Con A inhibits cell aggregation and prematurely induces cyclic AMP phosphodiesterase. Capping of Con A receptors has also been reported (8). Reitherman et al. (9) have recently reported that agglutination of cells by several plant lectins and the slime mold agglutination, discoidin, changes during development. Such studies indicate that differences in surface properties exist for cells at various stages of development. However, owing to the uncertainties in the factors which contribute to lectin-induced cell agglutination (10), the molecular basis for these observations remain to be determined. In this study, we have used microspheres (11-14) coupled to either Con A or wheat germ agglutinin (WGA) as visual markers to study by scanning electron microscopy the topographical distribution of lectin receptors on D. discoideum cells fixed at different stages of development. We also describe the effect of labeling on the distribution of lectin receptors and on the morphology of the cell surface.

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Scanning Electron Microscopy of Cell Surface Morphology

Current Protocols in Cell Biology ◽

10.1002/0471143030.cb0417s37 ◽

2007 ◽

Vol 37 (1) ◽

Cited By ~ 13

Author(s):

Samantha Passey ◽

Stéphanie Pellegrin ◽

Harry Mellor

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Cell Surface ◽

Surface Morphology ◽

Cell Surface Morphology ◽

Scanning Electron

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Mericarp morphology of the tribe Selineae (Apiaceae, Apioideae) and its taxonomic implications in Korea

Bangladesh Journal of Plant Taxonomy ◽

10.3329/bjpt.v25i2.39524 ◽

2018 ◽

Vol 25 (2) ◽

pp. 175-186 ◽

Cited By ~ 1

Author(s):

Changyoung Lee ◽

Jinki Kim ◽

Ashwini M. Darshetkar ◽

Ritesh Kumar Choudhary ◽

Sang-Hong Park ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Morphological Characters ◽

Surface Pattern ◽

Taxonomic Implications ◽

Scanning Electron

Mericarp morphology of 24 taxa belonging to nine genera of the tribe Selineae (Family: Apiaceae) in Korea was studied by Scanning Electron Microscopy. UPGMA and NMDS analyses were performed based on 12 morphological characters. The mericarp surface characters like mericarp shape, rib number and shape, surface pattern, surface appendages and mericarp symmetry proved useful in distinguishing the genera of the tribe Selineae.

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