scholarly journals Speciation in the genera Anthericum and Chlorophytum (Asparagaceae) in Ethiopia—a molecular phylogenetic approach

Phytotaxa ◽  
2017 ◽  
Vol 297 (2) ◽  
pp. 139 ◽  
Author(s):  
Charlotte Sletten Bjorå ◽  
MARTE ELDEN ◽  
INGER NORDAL ◽  
ANNE K. BRYSTING ◽  
TESFAYE AWAS ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Molecular Data ◽  
Group Relations ◽  
Variation Patterns ◽  
Molecular Phylogenetic ◽  
Lowland Area ◽  
A New Species ◽  
Scanning Electron

Sister group relations of Ethiopian species of Anthericum and Chlorophytum and variation patterns in the C. gallabatense and C. comosum complexes were studied using molecular phylogenetic analyses, morphometrics, and scanning electron microscopy of seed surfaces. Results indicate that molecular data largely support previous morphological conclusions, and that speciation has occurred in Ethiopia at least three times in Anthericum and repeatedly within different subclades of Chlorophytum. Areas particularly rich in endemic species are the lowland area around Bale Mountains in SE Ethiopia and in the Beninshangul Gumuz regional state in W Ethiopia near the border to Sudan. A new species, Chlorophytum mamillatum Elden & Nordal, is described, and the names C. tordense and C. tetraphyllum are re-instated.

Integrative taxonomy of the Asian skinks Sphenomorphus stellatus (Boulenger, 1900) and S. praesignis (Boulenger, 1900) with the resurrection of S. annamiticus (Boettger, 1901) and the description of a new species from Cambodia

Zootaxa ◽  
2019 ◽  
Vol 4683 (3) ◽  
pp. 381-411 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L., JR. WOOD ◽  
EVAN S. H. QUAH ◽  
SHAHRUL ANUAR ◽  
NIKOLAY A. POYARKOV ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Color Pattern ◽  
Integrative Taxonomy ◽  
Peninsular Malaysia ◽  
Wildlife Sanctuary ◽  
Molecular Phylogenetic ◽  
Univariate Analyses ◽  
A New Species

Molecular phylogenetic analyses of the sister species Sphenomorphus stellatus and S. praesignis based on the mitochondrial genes 12S and 16S rRNA recover the former as paraphyletic with respect to the latter in that a specimen of S. stellatus from the type locality in Peninsular Malaysia is more closely related to S. praesignis than to Indochinese populations of S. stellatus. Furthermore, the phylogeny indicates that the Indochinese populations represent two species, thus resulting in four major lineages within this clade. These relationships are consistent with multivariate and univariate analyses of morphological and discrete color pattern data which statistically define and diagnose the four lineages and together with the molecular data, provide the foundation for robust, testable, species-level hypotheses. As such, S. stellatus is herein restricted to Peninsular Malaysia; S. annamiticus is resurrected for the circum-continental populations ranging through southeastern Thailand, southern Cambodia, and southern Vietnam; a new species—S. preylangensis sp. nov.—is described from an isolated mountain, Phnom Chi, from the Prey Lang Wildlife Sanctuary in central Cambodia; and the taxonomy of S. praesignis remains unchanged. The description of S. preylangensis sp. nov. underscores the necessity to conserve this remnant of lowland evergreen rainforest in the Prey Lang Wildlife Sanctuary. 

scholarly journals Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Shape ◽  
Ssu Rdna ◽  
The Body ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

A new species of Physaloptera (Nematoda: Spirurida) from Proechimys gardneri (Rodentia: Echimyidae) from the Amazon rainforest and molecular phylogenetic analyses of the genus

2019 ◽  
Vol 94 ◽  
Author(s):  
A. Maldonado ◽  
R.O. Simões ◽  
J. São Luiz ◽  
S.F. Costa-Neto ◽  
R.V. Vilela
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Amazon Rainforest ◽  
Ancestral State ◽  
Intermediate Hosts ◽  
The Status ◽  
Molecular Phylogenetic ◽  
History Of ◽  
A New Species

Abstract Nematodes of the genus Physaloptera are globally distributed and more than 100 species are known. Their life cycle involves insects, including beetles, cockroaches and crickets, as intermediate hosts. This study describes a new species of Physaloptera and reports molecular phylogenetic analyses to determine its relationships within the family Physalopteridae. Physaloptera amazonica n. sp. is described from the stomach of the caviomorph rodent Proechimys gardneri collected in the Amazon rainforest in the state of Acre, Brazil. The species is characterized by the male having the first and second pair of sessile papillae asymmetrically placed, lacking a median papilla-like protuberance between the third pairs of sessile papillae, differentiated by size and shape of the spicules, while females have four uterine branches. For both nuclear 18S rRNA and MT-CO1 gene-based phylogenies, we recovered Turgida sequences forming a clade nested within Physaloptera, thus making Physaloptera paraphyletic to the exclusion of Turgida, suggesting that the latter may have evolved from the former monodelphic ancestral state to a derived polydelphic state, or that some species of Physaloptera may belong to different genera. Relationships between most taxa within Physaloptera were poorly resolved in our phylogenies, producing multifurcations or a star phylogeny. The star-like pattern may be attributed to evolutionary processes where past simultaneous species diversification events took place. Physaloptera amazonica n. sp. formed an independent lineage, separately from the other species of Physaloptera, thus supporting the status of a new species. However, all molecular data suggested a closer relationship with other Neotropical species. In conclusion, we added a new species to this already largely diverse genus Physaloptera, bringing new insights to its phylogenetic relationships. Further analyses, adding more species and markers, should provide a better understanding of the evolutionary history of physalopterids.

Description of Tottonophyes enigmatica gen. nov., sp. nov. (Hydrozoa, Siphonophora, Calycophorae), with a reappraisal of the function and homology of nectophoral canals

Zootaxa ◽  
2018 ◽  
Vol 4415 (3) ◽  
pp. 452 ◽  
Author(s):  
P. R. PUGH ◽  
C.W. DUNN ◽  
S.H.D. HADDOCK
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
The Other ◽  
Phylogenetic Position ◽  
Unique Combination ◽  
New Family ◽  
Molecular Phylogenetic ◽  
Distinct Morphology ◽  
A New Species

A new species of calycophoran siphonophore, Tottonophyes enigmatica gen. nov, sp. nov., is described. It has a unique combination of traits, some shared with prayomorphs (including two rounded nectophores) and some with clausophyid diphyomorphs (the nectophores are dissimilar, with one slightly larger and slightly to the anterior of the other, and both possess a somatocyst). Molecular phylogenetic analyses indicate that the new species is the sister group to all other diphyomorphs. A new family, Tottonophyidae, is established for it. Its phylogenetic position and distinct morphology help clarify diphyomorph evolution. The function and homology of the nectophoral canals and somatocyst is also re-examined and further clarification is given to their nomenclature.

scholarly journals A new species of Phrynops from Southern Brazil (Testudines, Chelidae), with comments on the phylogeny of the genus

2015 ◽  
Author(s):  
Natália Rizzo Friol ◽  
Flávio de Barros Molina ◽  
Hussam El Dine Zaher
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Sister Group ◽  
Molecular Data ◽  
Valid Species ◽  
Southern Brazil ◽  
Group Relationships ◽  
A New Species ◽  
Combined Data

Background. Phrynops present four valid species, including P. geoffroanus that might represents a complex of cryptic species. Here, we provide a preliminary analysis of the taxonomy and phylogenetic affinities within Phrynops, with special reference to the taxonomic status of populations of P. geoffroanus and P. tuberosus, and the recognition of a new species from Southern Brazil. Methods. We studied populations from ten Brazilian river basins. A linear morphometric analysis was performed in order to define taxonomically distinct populations. Also, a phylogenetic analysis using morphology and molecular data (sequenced for the genes R35, RAG2, c-mos, cytb, ND4, and 12S) were carried out. Three distinct sets of phylogenetic analyses were performed: parsimony to morphological and combined data, and maximum likelihood to molecular data. Results. The combined analysis shows that Phrynops represents a well supported clade. The set of skeletal data supports Mesoclemmys as the sister group of Phrynops, whereas the molecular and combined data sets show Phrynops as the sister group of a clade composed by all the remaining genera of Chelidae, except Hydromedusa. Our morphological analyses suggest that P. hilarii is the sister group of P. geoffroanus, but in both molecular and combined analyses, P. hilarii appears nested within the clade formed by the populations of P. geoffroanus. Futhermore, P. tuberosus and P. geoffroanus are not distinguishable by the set of osteological and morphometric data. On the other hand, both morphometric and osteological data show that the population of P. geoffroanus from the Paraná river basin is a distinct species. Discussion. The sister group relationships of Phrynops could not be clearly defined due to the different topologies achieved. Phrynops hilarii is included within of P. geoffroanus in both molecular and combined data, but this position has little statistical support and therefore does not express a clear position of P. hilarii within the genus Phrynops. Besides, we were not able to distinguish P. geoffroanus and P. tuberosus. However, a sampling of specific locations are still needed to objectively define the taxonomic status of P. tuberosus. Finally, the population of P. geoffroanus from the Paraná basin is clearly distinct from the remaining populations of this species. Qualitative osteological characters and morphometric results seem to demonstrate that this population is a new species of Phrynops.

The formerly enigmatic Unidentiidae in the limelight again: a new species of the genus Unidentia from Thailand (Gastropoda: Nudibranchia)

Zootaxa ◽  
2019 ◽  
Vol 4551 (5) ◽  
pp. 556 ◽  
Author(s):  
TATIANA KORSHUNOVA ◽  
RAHUL MEHROTRA ◽  
SPENCER ARNOLD ◽  
KENNET LUNDIN ◽  
BERNARD PICTON ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Morphological Data ◽  
Sea Slugs ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
First Time ◽  
A New Species

An integrative molecular and morphological study is presented for the family Unidentiidae. Molecular phylogenetic analyses were conducted with the inclusion of all previous and newly obtained molecular data for the family Unidentiidae Millen & Hermosillo 2012. A new species of the genus Unidentia Millen & Hermosillo 2012, U. aliciae sp. nov., is described from Thailand as part of an inventory of sea slugs at Koh Tao. All up-to-date available morphological data for the species of the genus Unidentia is for the first time summarized. Morphological differences among the different species of Unidentia are clarified showing that every species has its own distinguishable morphological traits. According to the new molecular and morphological data, the family Unidentiidae is re-confirmed as a well-supported taxon of the aeolidacean nudibranchs. The taxonomy and phylogeny of the Aeolidacea in the light of the family Unidentiidae is briefly discussed and necessity of a fine-scale and narrowly-defined taxa approach instead of a ‘‘superlumping’’ one is highlighted. 

scholarly journals Type studies of Rossbeevera bispora, and a new species of Rossbeevera from south China

MycoKeys ◽  
2019 ◽  
Vol 51 ◽  
pp. 15-28
Author(s):  
Md. Iqbal Hosen ◽  
Xiang-Jing Zhong ◽  
Genevieve Gates ◽  
Takamichi Orihara ◽  
Tai-Hui Li
Keyword(s):  
New Species ◽  
Nature Reserve ◽  
Phylogenetic Analyses ◽  
Guangdong Province ◽  
Molecular Data ◽  
Molecular Phylogenetic ◽  
Type Studies ◽  
A New Species ◽  
Bluish Discoloration ◽  
Northern And Southern Hemispheres

The type of Rossbeeverabispora and additional collections from the type location and adjacent areas were studied. Molecular data for R.bispora derived from the new collections are provided. In addition, R.griseobrunnea, a new species of Rossbeevera, is described from Xiangtoushan National Nature Reserve, Guangdong Province of China. The new species is characterized by its globose to subglobose sequestrate basidiomata with grayish white to grayish brown pileus, pale bluish discoloration in some parts of the hymenophore when injured becoming rusty brown to dark brown after being exposed to the air, fusoid (star-shaped in cross section) basidiospores 17–20 × 9–12 μm, and subcutis elements in the pileus. Based on multi-locus (ITS+nrLSU+tef1-α+rpb2) molecular phylogenetic analyses, both species appear as sister to R.paracyanea. We present color photos, macro- and micro-description, SEM basidiospores, molecular affinities of the species and compare them with morphologically similar taxa within the genus. A key to the species known from northern and southern hemispheres is provided.

A new pratylenchid nematode, Hoplotylus japonicus n. sp. (Tylenchina: Pratylenchidae) from the rhizosphere of Japanese maple, Acer palmatum (Thumb.)

Nematology ◽  
2020 ◽  
Vol 22 (10) ◽  
pp. 1131-1140
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Anterior Margin ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Dorsal Side ◽  
Lateral View ◽  
Molecular Phylogenetic ◽  
A New Species

Summary During quarantine inspections, a new species of Hoplotylus was detected from the rhizosphere of the Japanese maple, Acer palmatum, imported into China from Japan. Hoplotylus japonicus n. sp. is characterised by a stout 431-602 μm long body, well-developed 20.0-24.5 μm long stylet with tulip-shaped knobs having rounded sides in lateral view and a rounded anterior margin, excretory pore located slightly posterior to the hemizonid, usually empty, spermatheca, conical tail with a spike-like projection, which is distally smooth on the dorsal side, and males not found. It was morphologically compared with four currently known species of the genus, viz., H. femina, H. montanus, H. silvaticus, and H. sjacobi. In molecular phylogenetic analyses using 18S, 28S and internal transcribed spacer (ITS) ribosomal DNA sequences, the new species formed a clade with the only currently sequenced species of the genus, H. femina, in all phylogenies. This is the second species of the genus characterised by molecular data and the second species recovered from Japan.

scholarly journals New Light on Historical Specimens Reveals a New Species of Ladybird (Coleoptera: Coccinellidae): Morphological, Museomic, and Phylogenetic Analyses

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 766
Author(s):  
Karen Salazar ◽  
Romain Nattier
Keyword(s):  
New Species ◽  
Biological Diversity ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Protein Coding ◽  
Total Dna ◽  
Next Generation Sequencing Ngs ◽  
A New Species

Natural history collections house an important source of genetic data from yet unexplored biological diversity. Molecular data from museum specimens remain underexploited, which is mainly due to the degradation of DNA from specimens over time. However, Next-Generation Sequencing (NGS) technology can now be used to sequence “old” specimens. Indeed, many of these specimens are unique samples of nomenclatural types and can be crucial for resolving systematic or biogeographic scientific questions. Two ladybird beetle specimens from Patagonia corresponding to a new species of the genus Eriopis Mulsant were found in the collections of the Muséum national d’Histoire naturelle (MNHN), Paris. Here, we describe Eriopis patagonia Salazar, sp. nov. Total DNA of one of the two specimens was sequenced by NGS using a paired-end Illumina approach. We reconstruct and characterize the mitochondrial genome of this species (16,194 bp). Then, the protein-coding genes (PCGs) and ribosomal RNAs (rRNAs) were used to infer by maximum likelihood and Bayesian Inference the phylogenetic position of E. patagonia among 27 representatives of Coccinellidae. Phylogenetic analysis confirmed the position of Eriopis as sister group to Cycloneda Crotch. Hence, we highlight the high potential of sequencing technology for extracting molecular information from old specimens, which are used here for the systematic study of a genus, while demonstrating the importance of preserving biological collections.

scholarly journals Amanita ahmadii, a new species of Amanita subgenus Amanitina section Validae from Pakistan

MycoKeys ◽  
2019 ◽  
Vol 56 ◽  
pp. 81-99
Author(s):  
Sana Jabeen ◽  
Munazza Kiran ◽  
Junaid Khan ◽  
Ishtiaq Ahmad ◽  
Habib Ahmad ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Coniferous Forests ◽  
New Taxon ◽  
Data Set ◽  
Molecular Phylogenetic ◽  
A New Species

A new species from coniferous forests in Pakistan, Amanitaahmadii, is described on the basis of morpho-anatomy and molecular data set analyses. This species is characterized by its medium-sized to large basidiomata, grayish brown to brown pileal surface and rimose pileus margin with gray to dark brown verrucose veil remnants, a cream stipe with bulbous base having grayish brown or brown longitudinal striations above the annulus, a scaly surface towards the base, globose to broadly ellipsoid and amyloid basidiospores, and the absence of clamped septa in all tissues. Molecular phylogenetic analyses based on ITS and LSU sequences confirmed its identity as a new taxon nested within subgen. Amanitinasect.Validae.

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