Colletorichum serranegrense sp. nov., a new endophytic species from the roots of the endangered Brazilian epiphytic orchid Cattleya jongheana

Phytotaxa ◽  
2018 ◽  
Vol 351 (2) ◽  
pp. 163 ◽  
Author(s):  
MEIRIELE DA SILVA ◽  
EVERALDO S. CRUZ ◽  
TOMÁS G. R. VELOSO ◽  
LETÍCIA MIRANDA ◽  
OLINTO L. PEREIRA ◽  
...  

A new endophytic species of Colletotrichum was isolated from an endangered orchid species, Cattleya jongheana in the Parque Estadual da Serra Negra, from the state of Minas Gerais, Brazil. It is described and illustrated as Colletotrichum serranegrense sp. nov. in this study. Morphology and multi-locus phylogenetic analyses based on combined ITS, ACT, TUB2 and CHS-1 sequence data revealed that these isolates were in the Colletotrichum gigasporum species complex and confirmed differences in this compared to other species in this genus.

Phytotaxa ◽  
2014 ◽  
Vol 188 (2) ◽  
pp. 78 ◽  
Author(s):  
Nelson Menolli Jr. ◽  
ALFREDO JUSTO ◽  
PEDRO ARRILLAGA ◽  
C. K. PRADEEP ◽  
ANDREW M. MINNIS ◽  
...  

In order to better understand species delimitation in the Pluteus glaucotinctus species complex, we present a detailed study based on morphological and DNA sequence (nrITS + tef1) data. Pluteus glaucotinctus sensu stricto is known only from the type collection (Democratic Republic of the Congo), which is re-examined here. Four other species are recognized in this complex and described herein as new: P. izurun from Spain, P. meridionalis from Brazil, P. padanilus from India and P. thoenii from Africa. Phylogenetic analyses based on nrITS + tef1 sequence data support the recognition of all species in this complex.


2016 ◽  
Vol 54 (11) ◽  
pp. 2813-2819 ◽  
Author(s):  
Kerry O'Donnell ◽  
Deanna A. Sutton ◽  
Nathan Wiederhold ◽  
Vincent A. R. G. Robert ◽  
Pedro W. Crous ◽  
...  

Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.


Phytotaxa ◽  
2020 ◽  
Vol 450 (3) ◽  
pp. 273-284
Author(s):  
FA ZHANG ◽  
SHUO-RAN LIU ◽  
XIN-JUAN ZHOU ◽  
JUTAMART MONKAI ◽  
SINANG HONGSANAN ◽  
...  

Fusarium xiangyunensis sp. nov., isolated from hot-spring waterlogged soil in China, which is an endoparasitic fungus of nematodes, is described and illustrated. Evidence for the new species is provided by morphology and phylogenetic analyses of combined ITS, EF-1α and RPB1 sequence data. Phylogenetic analyses showed that F. xiangyunensis clustered with those species belonging to the F. solani species complex and grouped together with F. witzenhausenense. This species differs from F. witzenhausenense in habitat (parasitic on Hibiscus sp.), conidia and chlamydospores. Furthermore, the nematicidal activity of its microconidia was determined and causes infection and death of nematodes in both sterile water and solid medium.


MycoKeys ◽  
2021 ◽  
Vol 85 ◽  
pp. 57-71
Author(s):  
Taichang Mu ◽  
Zhaoxue Zhang ◽  
Rongyu Liu ◽  
Shubin Liu ◽  
Zhuang Li ◽  
...  

Colletotrichum has numerous host range and distribution. Its species are important plant pathogens, endophytes and saprobes. Colletotrichum can cause regular or irregular depressions and necrotic lesions in the epidermal tissues of plants. During this research Colletotrichum specimens were collected from Mengyin County, Shandong Province, China. A multi-locus phylogenetic analysis of ITS, GAPDH, CHS-1, ACT, TUB2, CAL and GS sequence data combined with morphology, revealed a new species and two known species, viz. C. mengyinense sp. nov., C. gloeosporioides and C. pandanicola, belonging to the C. gloeosporioides species complex. The new species is described and illustrated in this paper and compared with taxa in the C. gloeosporioides species complex.


Phytotaxa ◽  
2019 ◽  
Vol 387 (2) ◽  
pp. 149-157 ◽  
Author(s):  
GUO-QIANG ZHANG ◽  
GUI-ZHEN CHEN ◽  
LI-JUN CHEN ◽  
SI-REN LAN

A new orchid species, Cymbidium yunnanensis (Orchidaceae; Epidendroideae; Cymbidieae), from Yunnan Province, China, is described and illustrated. Based on morphological and molecular analyses, we demonstrate that this new species is similar to C. floribundum, but it differs in its smaller size, leaves 5–15 cm, shorter scape, white flowers with pale purplish markings on its sepals and petals, lip with purplish red blotch, petals narrowly falcate oblong and lip midlobe nearly round with column foot 1.5–2.0 mm long. Phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) DNA sequence data support C. yunnanensis as a new species.


Phytotaxa ◽  
2015 ◽  
Vol 239 (2) ◽  
pp. 130 ◽  
Author(s):  
QI ZHAO ◽  
TOLGOR B ◽  
YONGCHANG ZHAO ◽  
ZHULIANG YANG ◽  
KEVIN D. HYDE

The white saddle-mushroom, a well-known European species, has long been named as Helvella crispa in China. In this study, phylogenetic analyses of combined ITS, nrLSU, tef1-α, rpb2 and mcm7 sequence data, showed that the Chinese H. crispa-like samples represent a species complex, which contains at least six phylogenetic species. Three of these species, H. involuta, H. orienticrispa and H. pseudoreflexa, are introduced as new species in this paper. The remaining two taxa are not described due to paucity of material. Among the six phylogenetic species, H. zhongtiaoensis is more closely related to European H. crispa than the remaining Chinese species. They are provided with descriptions, photographs and are compared with similar species. A key to the Chinese morphologically recognizable taxa of this complex is provided.


Phytotaxa ◽  
2021 ◽  
Vol 491 (2) ◽  
pp. 143-157
Author(s):  
NAPALAI CHAIWAN ◽  
SAOWALUCK TIBPROMMA ◽  
RUVISHIKA S. JAYAWARDENA ◽  
AUSANA MAPOOK ◽  
DHANUSHKA N. WANASINGHE ◽  
...  

During an investigation into the microfungi on Dracaena fragrans in Thailand, a saprobic taxon belonging to Glomerellaceae was collected. Based on morphological characterization and combined phylogenetic analyses of ITS, GAPDH, CHS-1, ACT and TUB2 sequence data from the sexual and asexual morphs our taxon was identified as a new species of Colletotrichum. Colletotrichum dracaenigenum sp. nov. is introduced here with a full description, colour photographs of morphological characteristics and a phylogenetic tree to show the placement of the new taxon in the gloeosporioides species complex.


2013 ◽  
Vol 58 (2) ◽  
Author(s):  
Yanzhen Bu ◽  
Hongxing Niu ◽  
Luping Zhang

AbstractSeven species of Cylicocyclus Ihle, 1922 (Nematoda: Strongylidae) were collected from donkeys from Henan Province, China. Five samples of each species were selected for sequencing. Sixteen different internal transcribed spacer (ITS) sequences representing the seven species of Cylicocyclus were obtained. Sequence differences in the first internal transcribed spacer (ITS-1) among species was lower than that of the second internal transcribed spacer (ITS-2). Phylogenetic analyses were conducted using the combined ITS-1 and ITS-2 data sets from the present study and using reference sequences from the GenBank database. The MP and ML trees were similar in topology. The phylogenetic trees were divided into two clades. Clade I included 8 species of Cylicocyclus; within this group, Cylicocyclus leptostomus (Kotlan, 1920) is nested between different samples of Cylicocyclus ashworthi (LeRoux, 1924), suggesting C. ashworthi may represent a species complex. Clade II included Cylicocyclus elongatus (Looss, 1900) and Cylicocyclus ultrajectinus (Ihle, 1920); however, these two species always clustered with the comparative species (Petrovinema poculatum (Looss, 1900) and Poteriostomum imparidentatum Quiel, 1919), suggesting that C. elongatus and C. ultrajectinus represent members of other genera.


Parasitology ◽  
2019 ◽  
Vol 146 (13) ◽  
pp. 1673-1682 ◽  
Author(s):  
Tanapan Sukee ◽  
Ian Beveridge ◽  
Neil B. Chilton ◽  
Abdul Jabbar

AbstractThe genetic variation and taxonomic status of the four morphologically-defined species of Macropostrongyloides in Australian macropodid and vombatid marsupials were examined using sequence data of the ITS+ region (=first and second internal transcribed spacers, and the 5.8S rRNA gene) of the nuclear ribosomal DNA. The results of the phylogenetic analyses revealed that Ma. baylisi was a species complex consisting of four genetically distinct groups, some of which are host-specific. In addition, Ma. lasiorhini in the common wombat (Vombatus ursinus) did not form a monophyletic clade with Ma. lasiorhini from the southern hairy-nosed wombat (Lasiorhinus latifrons), suggesting the possibility of cryptic (genetically distinct but morphologically similar) species. There was also some genetic divergence between Ma. dissimilis in swamp wallabies (Wallabia bicolor) from different geographical regions. In contrast, there was no genetic divergence among specimens of Ma. yamagutii across its broad geographical range or between host species (i.e. Macropus fuliginosus and M. giganteus). Macropostrongyloides dissimilis represented the sister taxon to Ma. baylisi, Ma. yamagutii and Ma. lasiorhini. Further morphological and molecular studies are required to assess the species complex of Ma. baylisi.


Phytotaxa ◽  
2019 ◽  
Vol 423 (2) ◽  
pp. 87-92
Author(s):  
XING-YU LIAO ◽  
XUE-DIE LIU ◽  
YU-TING JIANG ◽  
DI-YANG ZHANG ◽  
SI-REN LAN ◽  
...  

A new orchid species, Cymbidium atrolabium, from Yunnan Province, China, is described and illustrated based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of Cymbidium was conducted. The new plant is similar to C. tortisepalum but differs by having a dark-purple inflorescence with smaller flowers, sepals that are pale green-yellow with a dark-purple broad midrib, petals that are dark-purple with pale green-yellow margins and a smaller, dark purple, densely papillose lip. Phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) sequence data support the status of C. atrolabium as a new species.


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