scholarly journals New Zealand species of the genus Tripyla Bastian, 1865 (Nematoda:Triplonchida: Tripylidae). I : A new species, a new record and key to long-tailed species

Zootaxa ◽  
2021 ◽  
Vol 2291 (1) ◽  
Author(s):  
ZENG QI ZHAO
Keyword(s):  
New Zealand ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
Long Tail ◽  
Using Data ◽  
De Man ◽  
First Time ◽  
Short Body ◽  
A New Species

This paper describes two species of the genus of Tripyla from New Zealand and also provides a key to species based on the morphology of females in eight long-tailed (c < 5) species in the genus of Tripyla. Tripyla bioblitz sp. nov. is characterized by its more anterior vulva position (V = 43.7–45.4%), relatively short body length (1150–1410 μm) and long tail (c = 4.0–4.4) in the group. Tripyla filicaudata de Man, 1880 is recorded for the first time from New Zealand and from the Southern Hemisphere. In addition, the phylogenetic relationships among species were analyzed using data from the near full length small subunit (SSU) and D2/D3 expansion segments of the large subunit ribosomal (LSU) rRNA genes, and these analyses revealed that T. bioblitz sp. nov. is close to but distinct from T. filicaudata de Man, 1880.

Morphological and molecular characteristics of Bastiania sinensis sp. n., Tripyla aquatica Brzeski & Winiszewska-Ślipińska, 1993 and T. setifera Bütschli, 1873 (Nematoda: Triplonchida) from Shanxi Province, North China

Nematology ◽  
2021 ◽  
pp. 1-23
Author(s):  
Mei Na Liu ◽  
Yu Mei Xu ◽  
Zeng Qi Zhao ◽  
Jian Ming Wang
Keyword(s):  
North China ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
Shanxi Province ◽  
Molecular Characteristics ◽  
Separate Species ◽  
Using Data ◽  
First Time ◽  
Respective Species

Summary This paper describes a new species of Bastiania, presents a new record and redescribes a known species of Tripyla. These nematodes are all in the order Triplonchida and were collected from Shanxi Province, North China. Bastiania sinensis sp. n. is characterised by having the female with a relatively slender body 1049-1295 μm long, dorsally arcuate after heat relaxation, with outer labial setae and cephalic setae in a single circle, an oval amphid, 7-8 laterodorsal cervical setae scattered in the pharyngeal region, orthometamenes and pseudocoelomocytes present, tail conoid with a mucron 1-2 μm long, two pairs of caudal setae present, a = 58.1-75.5, b = 4.0-4.6, c = 12.7-19.7, c′ = 4.1-7.8 and V = 61.1-67.7. Males were not found. Tripyla aquatica is recorded for the first time from China, and is redescribed. Tripyla setifera has been reported from China but without a detailed description – now provided. In addition, phylogenetic relationships among the species were analysed using data from the near full length small subunit (SSU) and D2-D3 segments of large subunit (LSU) of rRNA genes. Bastiania sinensis sp. n. is monophyletic with the Bastiania sequences available in GenBank, but is on an independent branch supporting its status as a separate species; T. aquatica and T. setifera are monophyletically clustered with known Tripyla species and grouped together with sequences from their respective species.

Schistonchus zealandicus n. sp. (Nematoda: Aphelenchoididae) associated with Ficus macrophylla in New Zealand

Nematology ◽  
2015 ◽  
Vol 17 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Zengqi Zhao ◽  
Dongmei Li ◽  
Kerrie A. Davies ◽  
Weimin Ye
Keyword(s):  
New Zealand ◽  
Large Subunit ◽  
Tail Length ◽  
Small Subunit ◽  
Rrna Genes ◽  
Lateral Field ◽  
Male Tail ◽  
Tail Tip ◽  
Large Subunit Rrna ◽  
A New Species

Schistonchus zealandicus n. sp. was recovered from the sycones of Ficus macrophylla from St Helier’s Bay, Mt Wellington, and St Johns, suburbs of Auckland, New Zealand. It is characterised by having the opening of the excretory pore located just posterior to the anterior end of the nematode but anterior to the conus level of the stylet, a short post-vulval uterine sac (10-18 μm or 0.4-0.7 vulval body diam. long), presence of two incisures in the lateral field with many broken, non-defined lines between them, rose-thorn-shaped spicule, three pairs of subventral papillae on the male tail (one pair adcloacal on the anterior lip, one pair slightly posterior to mid-tail length, and one pair close to tail tip), a distinctive mucron on the male tail, apparent biogeographical range, and its association with F. macrophylla. The absence of a lip sector disc suggests that it is close to S. altermacrophylla, S. aureus and S. laevigatus. Molecular phylogeny of near full length small subunit and D2-D3 expansion segments of the large subunit rRNA genes supports the proposal of S. zealandicus n. sp. as a new species.

Litylenchus coprosma gen. n., sp. n. (Tylenchida: Anguinata), from leaves of Coprosma repens (Rubiaceae) in New Zealand

Nematology ◽  
2011 ◽  
Vol 13 (1) ◽  
pp. 29-44 ◽  
Author(s):  
Kerrie Davies ◽  
Zeng Qi Zhao ◽  
Brett Alexander ◽  
Ian Riley
Keyword(s):  
New Zealand ◽  
New Genus ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
New Genus And Species ◽  
Pore Opening ◽  
Terminal Bulb ◽  
Stylet Length ◽  
Tail Tip

AbstractA new genus and species of anguinid nematode, Litylenchus coprosma gen. n., sp. n., was recovered from leaves of Coprosma repens A. Rich. from an amenity planting in Wellington, New Zealand. The genus is characterised by having slender males and slender or semi-obese females, pharynx with a weak non-muscular median bulb, a terminal bulb containing the pharyngeal glands, female with a single gonad having a quadricolumella and post-uterine sac; male with arcuate spicules and the bursa arising 1-2 anal body diam. anterior to the cloacal aperture and extending nearly to the tail tip, and does not induce galls, only foliar chlorosis. The species is characterised by having a short, robust stylet with conus forming ca 40% of stylet length and three well developed rounded knobs, secretory/excretory pore opening posterior to the nerve ring, terminal bulb abutting the intestine, and tail tip of variable form. Molecular phylogeny of near full length small subunit, D2/D3 expansion segments of the large subunit and internal transcribed spacer rRNA genes support the description of L. coprosma gen. n., sp. n. as a new genus and species.

Latronema whataitai sp. n. (Nematoda: Selachinematidae) from intertidal sediments of New Zealand, with notes on relationships within the family based on preliminary 18S and D2-D3 phylogenetic analyses

Nematology ◽  
2015 ◽  
Vol 17 (8) ◽  
pp. 941-952 ◽  
Author(s):  
Daniel Leduc ◽  
Zeng Zhao
Keyword(s):  
New Zealand ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
Intertidal Sediments ◽  
Molecular Sequences ◽  
Close Relationship ◽  
Central Tooth ◽  
Family Based

Latronema whataitai sp. n. is described from intertidal sediments of Hataitai Beach, North Island of New Zealand, and its placement within the Selachinematidae investigated using 18S and D2-D3 molecular sequences. Latronema whataitai sp. n. is characterised by relatively slender body (a = 22-25), outer labial setae and cephalic setae of similar length, anterior buccal cavity with three sets of seven teeth with larger central tooth positioned above the other six, circular amphid with faint outline, males with 12-13 precloacal supplements and spicules with pointed projection near distal end, and tail 2.0-2.6 anal body diam. long. The cuticle ultrastructure of the genus is investigated for the first time using scanning electron microscopy. Molecular phylogenies of near full length small subunit and D2-D3 expansion segments of the large subunit rRNA genes do not provide support for the division of Selachinematidae into the subfamilies Selachinematinae and Choniolaiminae, and do not support a close relationship between Latronema and Richtersia despite some morphological similarities. These preliminary results are based on a small number of molecular sequences, and therefore the current morphology-based classification remains the most tractable system for the Selachinematidae until more comprehensive analyses are conducted.

A new species of the genus Tripylina Brzeski, 1963 (Nematoda: Enoplida: Trischistomatidae) from Shanxi province, China

Zootaxa ◽  
2013 ◽  
Vol 3630 (3) ◽  
pp. 561-570 ◽  
Author(s):  
YU MEI XU ◽  
ZENG QI ZHAO ◽  
JIAN MING WANG ◽  
JING WU ZHENG
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Ventral Side ◽  
Small Subunit ◽  
Shanxi Province ◽  
Cervical Region ◽  
Large Subunit Rdna ◽  
Using Data ◽  
A New Species ◽  
Shape And Size

Tripylina puxianensis n. sp. is described and illustrated from Shanxi province, China. It is characterized by having a triangular dorsal tooth, pointing towards the ventral side, anterior to two sub-ventral teeth, with dorsal and sub-ventral teeth similar in shape and size; one ventromedian seta in the cervical region; vulva with protruding lips, absence of post-uterine sac; and a pair of setae on the tail; male not found; female body length 1048–1331 μm, a = 25.5–33.8, b = 5.5–6.2, c = 14.4–23.1, c' = 2.1–3.5 and V = 56.1–68.5%. The phylogenetic relationships among species in the genus Tripylina were analyzed using data from the SSU (small subunit) and D2/D3 expansion segments of the LSU (large subunit) rDNA. These analyses confirmed that T. puxianensis n. sp. is different from other members of the genus for which sequences are available.

Zeatylenchus pittosporum gen. n., sp. n. (Tylenchida: Anguinata), from leaves of Pittosporum tenuifolium (Pittosporaceae) in New Zealand

Nematology ◽  
2013 ◽  
Vol 15 (2) ◽  
pp. 197-212
Author(s):  
Zeng Qi Zhao ◽  
Kerrie A. Davies ◽  
Brett J.R. Alexander ◽  
Ian T. Riley
Keyword(s):  
New Zealand ◽  
New Genus ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
New Genus And Species ◽  
Pore Opening ◽  
Males And Females ◽  
Stylet Length ◽  
Tail Tip

A new genus and species of anguinid nematode, Zeatylenchus pittosporum gen. n., sp. n., was recovered from leaves of Pittosporum tenuifolium from Hahei, Coromandel Region, North Island, New Zealand. The genus is characterised by having slender males and females, excretory pore opening near the lips and level with the knobs of the retracted stylet, pharynx with a weak non-muscular median bulb, pharyngeal glands overlapping the intestine, females with a single gonad with a quadricolumella and post-uterine sac; and males with slender arcuate spicules and the bursa arising <1 anal body diam. anterior to the cloacal aperture and extending ca 30% of distance to the tail tip. Its feeding does not induce galls, only foliar chlorosis. The species has particular characters, including a short, robust stylet with conus forming ca 40% of stylet length and small rounded compact knobs, and tail offset dorsally with a pointed tip. Molecular phylogeny of near full length small subunit, D2/D3 expansion segments of the large subunit and internal transcribed spacer rRNA genes support the description of Zeatylenchus pittosporum gen. n., sp. n. as a new genus and species.

Theileria parva ribosomal internal transcribed spacer sequences exhibit extensive polymorphism and mosaic evolution: application to the characterization of parasites from cattle and buffalo

Parasitology ◽  
1999 ◽  
Vol 118 (6) ◽  
pp. 541-551 ◽  
Author(s):  
N. E. COLLINS ◽  
B. A. ALLSOPP
Keyword(s):  
Genetic Recombination ◽  
Large Subunit ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Rrna Genes ◽  
Gene Pools ◽  
Theileria Parva ◽  
Causative Agents ◽  
Internal Transcribed Spacer Sequences

We sequenced the rRNA genes and internal transcribed spacers (ITS) of several Theileria parva isolates in an attempt to distinguish between the causative agents of East coast fever and Corridor disease. The small subunit (SSU) and large subunit (LSU) rRNA genes from a cloned T. p. lawrencei parasite were sequenced; the former was identical to that of T. p. parva Muguga, and there were minor heterogeneities in the latter. The 5·8S gene sequences of 11 T. parva isolates were identical, but major differences were found in the ITS. Six characterization oligonucleotides were designed to hybridize within the variable ITS1 region; 93·5% of T. p. parva isolates examined were detected by probe TPP1 and 81·8% of T. p. lawrencei isolates were detected by TPL2 and/or TPL3a. There was no absolute distinction between T. p. parva and T. p. lawrencei and the former hybridized with fewer of the probes than did the latter. It therefore seems that a relatively homogenous subpopulation of T. parva has been selected in cattle from a more diverse gene pool in buffalo. The ITSs of both T. p. parva and T. p. lawrencei contained different combinations of identifiable sequence segments, resulting in a mosaic of segments in any one isolate, suggesting that the two populations undergo genetic recombination and that their gene pools are not completely separate.

Contribution to the knowledge of the oribatid mite genus Leptotocepheus (Acari, Oribatida, Otocepheidae)

2018 ◽  
Vol 23 (11) ◽  
pp. 2269
Author(s):  
Sergey G. Ermilov ◽  
Maria Minor
Keyword(s):  
New Species ◽  
New Zealand ◽  
Oribatid Mite ◽  
Taxonomic Status ◽  
Identification Key ◽  
First Time ◽  
Australasian Region ◽  
A New Species ◽  
Mite Genus

The otocepheid oribatid mite genus Leptotocepheus Balogh, 1961 is recorded in the Australasian region for the first time. The taxonomic status of the genera Leptotocepheus Balogh, 1961 and Longocepheus Balogh & Mahunka,1966 is discussed, resulting in the recognition of Longocepheus as a subgenus (stat. nov.) of Leptotocepheus and the following recombinations: Leptotocepheus (Longocepheus) australis (Balogh & Mahunka, 1966) comb. nov., Leptotocepheus (Longocepheus) globosus (Grobler, 1995) comb. nov., Leptotocepheus (Longocepheus) longus (Balogh, 1961) comb. nov., Leptotocepheus (Longocepheus) youngai (Mahunka, 1984) comb. nov. A new species of Leptotocepheus (Longocepheus) is described from New Zealand, Leptotocepheus (Longocepheus) neozealandicus sp. nov., which differs from other species of the subgenus by the presence of short, slightly developed prodorsal costulae. Revised generic and subgeneric diagnoses, an identification key and distributions for the known taxa of Leptotocepheus are presented.

scholarly journals The caridean shrimps of the genus Lysmata Risso, 1816 (Decapoda: Lysmatidae) from Madagascar collected during the Atimo-Vatae expedition: a new species and two new records

2021 ◽  
Vol 774 ◽  
pp. 155-177
Author(s):  
Hossein Ashrafi ◽  
J. Antonio Baeza ◽  
Zdeněk Ďuriš
Keyword(s):  
New Species ◽  
New Records ◽  
Original Description ◽  
Western Atlantic ◽  
West Pacific ◽  
Phylogenetic Studies ◽  
The Caribbean ◽  
De Man ◽  
First Time ◽  
A New Species

The present study focuses on shrimps belonging to the genus Lysmata Risso, 1816, collected from Madagascar during the Atimo Vatae expedition carried out in 2010. Lysmata malagasy sp. nov. is a new species belonging to the clade named “long accessory ramous” or “cosmopolitan” in previous phylogenetic studies. The new species can be distinguished from the only two other representatives of this group in the Indo-west Pacific, L. ternatensis De Man, 1902, and L. trisetacea (Heller, 1861), by the accessory ramus of the lateral antennular flagellum consisting of four elongated articles. Lysmata lipkei Okuno & Fiedler, 2010 is reported here from Madagascar with a remarkable extension of its known range after its original description from Japan. This species has also been reported from Singapore and, as alien species, from Brazil. Lastly, L. kuekenthali De Man, 1902 known from numerous localities in the Indo-West Pacific biogeographic area, is reported for the first time from Madagascar. Results of the present morphological and molecular analyses suggest that L. hochi Baeza & Anker, 2008 from the Caribbean Sea is a synonym of the Indo-West Pacific L. kuekenthali, and thus the latter species is alien in the western Atlantic.

scholarly journals Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park

2002 ◽  
Vol 68 (10) ◽  
pp. 5123-5135 ◽  
Author(s):  
Carrine E. Blank ◽  
Sherry L. Cady ◽  
Norman R. Pace
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Hydrogen Oxidation ◽  
Large Subunit ◽  
Microbial Community Composition ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Rrna Genes ◽  
Small Subunit Rrna ◽  
Microbial Composition

ABSTRACT The extent of hyperthermophilic microbial diversity associated with siliceous sinter (geyserite) was characterized in seven near-boiling silica-depositing springs throughout Yellowstone National Park using environmental PCR amplification of small-subunit rRNA genes (SSU rDNA), large-subunit rDNA, and the internal transcribed spacer (ITS). We found that Thermocrinis ruber, a member of the order Aquificales, is ubiquitous, an indication that primary production in these springs is driven by hydrogen oxidation. Several other lineages with no known close relatives were identified that branch among the hyperthermophilic bacteria. Although they all branch deep in the bacterial tree, the precise phylogenetic placement of many of these lineages is unresolved at this time. While some springs contained a fair amount of phylogenetic diversity, others did not. Within the same spring, communities in the subaqueous environment were not appreciably different than those in the splash zone at the edge of the pool, although a greater number of phylotypes was found along the pool's edge. Also, microbial community composition appeared to have little correlation with the type of sinter morphology. The number of cell morphotypes identified by fluorescence in situ hybridization and scanning electron microscopy was greater than the number of phylotypes in SSU clone libraries. Despite little variation in Thermocrinis ruber SSU sequences, abundant variation was found in the hypervariable ITS region. The distribution of ITS sequence types appeared to be correlated with distinct morphotypes of Thermocrinis ruber in different pools. Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences.

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