The larva of Hydropsyche doehleri Tobias 1972, based on Swiss material (Hydropsychidae, Trichoptera)

Zootaxa ◽  
2020 ◽  
Vol 4786 (4) ◽  
pp. 535-545
Author(s):  
HEINRICH VICENTINI ◽  
SOFIA WYLER ◽  
JOHANN WARINGER
Keyword(s):  
Abdominal Segment ◽  
Sequence Data ◽  
Light Trap ◽  
Distribution Patterns ◽  
Adult Males ◽  
Diagnostic Features ◽  
Species Association ◽  
Dna Sequence Data ◽  
Males And Females ◽  
Region Information

This paper describes the previously unknown larva of Hydropsyche doehleri Tobias 1972. Species association was enabled by the fact that both larval and adult specimens were collected at the same location and that H. doehleri was the only Hydropsychidae collected at this site, based on light-trap samples of adults. In addition, association of larvae with adult males and females were performed using DNA sequence data from the mitochondrial cytochrome c oxidase region. Information on the morphology of the larva is given, and the most important diagnostic features separating H. doehleri from its sister taxon H. siltalai Döhler 1963 are discussed. In both species, gills are lacking on abdominal segment VII. In the context of the Hydropsychidae key of Waringer & Graf (2011), the two species can be separated by the morphology and coloration of the frontoclypeal apotome, and by their distribution patterns: whereas H. siltalai is widespread in Europe, H. doehleri has been reported from only France, Italy, and southern Switzerland (Tessin).                #We dedicate this paper to Univ. Prof. Dr Hans Malicky on the occasion of his 85th birthday. 

Phylogenetic relationships of Polysiphonia (Rhodomelaceae, Rhodophyta) and its relatives based on anatomical and nuclear small-subunit rDNA sequence data

2001 ◽  
Vol 79 (12) ◽  
pp. 1465-1476 ◽  
Author(s):  
Han-Gu Choi ◽  
Myung-Sook Kim ◽  
Michael D Guiry ◽  
Gary W Saunders
Keyword(s):  
North Atlantic ◽  
Sequence Data ◽  
Small Subunit ◽  
Total Evidence ◽  
Northwest Pacific ◽  
Diagnostic Features ◽  
Dna Sequence Data ◽  
The North ◽  
Small Subunit Rdna ◽  
The North Atlantic

The aim of this study was to reassess monophyly of the genus Polysiphonia and determine the phylogenetic affinities of its component lineages among related red algae belonging to the Rhodomelaceae. Our "total evidence" approach, combining 28 anatomical characters and small-subunit ribosomal DNA sequence data for 25 ceramialean algae including 14 species of Polysiphonia sensu lato (including two species of the recently described genus Neosiphonia) and nine related Rhodomelaceae, indicates that Polysiphonia sensu lato consists of three strongly supported clades, Polysiphonia group, Neosiphonia group, and a "multipericentral" group, and a single taxon lineage consisting of Womersleyella setacea. The type species of the genus, Polysiphonia urceolata (= Polysiphonia stricta) from the north Atlantic, formed a distinct clade with Polysiphonia morrowii and Polysiphonia pacifica from the northwest and northeast Pacific, respectively. The Neosiphonia group included Neosiphonia japonica and Neosiphonia savatieri from the northwest Pacific, as originally proposed, Polysiphonia harveyi from the north Atlantic, which shares diagnostic features with this genus, and the anomalous Polysiphonia elongata and Polysiphonia virgata from the north Atlantic and South Africa, respectively. Boergeseniella and Vertebrata from the north Atlantic and Enelittosiphonia from the northwest Pacific associated solidly with the multipericentral Polysiphonia fucoides and Polysiphonia nigra from the north Atlantic. The implications for the taxonomy of Polysiphonia sensu lato and related genera within the Rhodomelaceae are discussed.Key words: Neosiphonia, nuclear small-subunit rDNA, phylogeny, Polysiphonia, Rhodomelaceae, Rhodophyta, systematics.

Gyretes Brullé (Coleoptera: Gyrinidae) from Brazil: Morphology of eggs and early instars

Zootaxa ◽  
2018 ◽  
Vol 4526 (3) ◽  
pp. 331 ◽  
Author(s):  
DANIARA COLPANI ◽  
CÉSAR JOÃO BENETTI ◽  
NEUSA HAMADA ◽  
VANDERLY ANDRADE-SOUZA ◽  
MARIANO C. MICHAT
Keyword(s):  
Related Species ◽  
Sequence Data ◽  
Larval Survival ◽  
Molecular Techniques ◽  
Coi Gene ◽  
Immature Stages ◽  
Closely Related Species ◽  
Species Association ◽  
Dna Sequence Data ◽  
Laboratory Conditions

Taxonomic information regarding Gyrinidae is mostly based on adults, especially due to the difficulty in collecting immatures and assigning them to a particular species. Association between immatures and adults is sometimes difficult because closely related species can be found in the same habitat. To solve this problem a feasible technique is rearing under laboratory conditions. However, this method is challenging because larval survival rate is usually low, and emulation of natural conditions is difficult. Molecular techniques, especially the use of the COI gene, have been applied to identify species and to associate different life stages. However, in some species groups this marker has not been successful in distinguishing closely related species. The objectives of this study are to describe the egg and the first two instars of Gyretes nubilus Ochs, 1965 and the egg of G. minax Ochs, 1967 and to evaluate the utility of COI to associate immatures and adults. The association of these immature stages with adults was done either rearing adults under laboratory conditions or by using DNA sequence data (COI), corroborating the utility of this molecular marker to associate immature and adults in Gyretes. These immature stages are described, including chaetotaxic analysis of larvae for the first time for the genus Gyretes Brullé, 1835. The eggs are described based on scanning electron microscopy. The eggs are similar to those of other Gyrinidae genera in having a micropylar region in the anterior pole and a longitudinal fissure, and by the absence of an aeropyle, but they differ mainly in characters related to chorionic structure and reticulation. Larvae of Gyretes can be distinguished from those of the other Neotropical Gyrinidae genera by a combination of several characters, including the frontoclypeal seta FR3 short, presence of three conspicuous additional setae on lateral region of parietal (contiguous to stemmata), and posterior margin of lacinia smooth, with apex not indented. 

A new species of scincid lizard in the genus Caledoniscincus (Reptilia: Scincidae) from northwest New Caledonia

Zootaxa ◽  
2012 ◽  
Vol 3229 (1) ◽  
pp. 47 ◽  
Author(s):  
ROSS A. SADLIER ◽  
TONY WHITAKER ◽  
PERRY L. WOOD ◽  
AARON M. BAUER
Keyword(s):  
New Species ◽  
New Caledonia ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
Adult Males ◽  
Human Occupation ◽  
Dna Sequence Data ◽  
The North ◽  
Conservation Concern ◽  
A New Species

A new species of skink in the genus Caledoniscincus is described from the northwest region of New Caledonia. It is knownfrom two locations, one on the coast at Pointe de Vavouto north of Koné, the other on the slopes of the Massif d’Oua-zangou, an isolated mountain 30 km to the north. Typical of all members of the genus, adult males and females of the newspecies are sexually dichromatic. The new species, Caledoniscincus constellatus sp. nov., has a bold, white midlateralstripe, a feature which distinguishes it from most other species of Caledoniscincus except the regionally sympatric C. hap-lorhinus (Günther) and C. austrocaledonicus (Bavay), from which it can be distinguished by a unique pattern of contactbetween the pale midlateral stripe and the ear. DNA sequence data for the ND2 mitochondrial gene identifies a high levelof genetic differentiation between the new species and all other Caledoniscincus, further supporting its distinctiveness asan independent evolutionary lineage. The species is of high conservation concern given its restricted distribution in a re-gion that has been, and will continue to be, heavily impacted by human occupation, and would be ranked as Critically Endangered under IUCN criteria.

The larva of Plectrocnemia renetta Malicky 1975 (Trichoptera, Polycentropodidae), including a discriminatory matrix to the larvae of Plectrocnemia Stephens 1836 species of Greece

Zootaxa ◽  
2019 ◽  
Vol 4568 (2) ◽  
pp. 372
Author(s):  
JOHANN WARINGER ◽  
HANS MALICKY
Keyword(s):  
Abdominal Segment ◽  
Distribution Patterns ◽  
Head Capsule ◽  
Instar Larva ◽  
Muscle Attachment ◽  
Diagnostic Features ◽  
Of Greece

This paper describes the previously unknown larva of Plectrocnemia renetta Malicky 1975. Information on the morphology of the final instar larva is given and the most important diagnostic features are illustrated. A preliminary discriminatory matrix for the Greek larvae of Plectrocnemia Stephens 1836 is also provided. Plectrocnemia renetta and P. conspersa conspersa (Curtis 1834) belong to the group where the inner and outer dorsal secondary setae on abdominal segment IX are strongly different in length. These two species can be separated from each other by the arrangement of muscle attachment spots on the head capsule, number and length of setae on abdominal sternum IX, and by distribution patterns. With respect to zoogeography, Plectrocnemia renetta has been reported from Cyprus, Turkey, and from the Greek islands of Ikaria and Samos.

Description of nymphs and female subimago of Sparsorythus multilabeculatus Sroka & Soldán, 2008 (Ephemeroptera: Tricorythidae) associated with male imago based on DNA sequence data

Zootaxa ◽  
2019 ◽  
Vol 4695 (6) ◽  
pp. 501-515
Author(s):  
KANYAKORN PIRAONAPICHA ◽  
NARUMON SANGPRADUB
Keyword(s):  
National Park ◽  
Abdominal Segment ◽  
Sequence Data ◽  
Basal Area ◽  
Distal Portion ◽  
Polar Cap ◽  
Basal Segment ◽  
Dna Sequence Data ◽  
Dark Colour ◽  
First Time

Sparsorythus is a genus of Tricorythidae from the Oriental Region. Sparsorythus multilabeculatus Sroka & Soldán, 2008 was described based on a male imago from Vietnam. Unknown nymphs and female subimagines of Sparsorythus and male imagines of S. multilabeculatus were collected from Thap Lan National Park, Khon Buri District, Nakhon Ratchasima Province, Thailand. Nymphs and female subimagines of Sparsorythus were associated with male imagines of S. multilabeculatus by analyzing sequences from the DNA barcoding region of the mitochrondrial gene cytochrome oxidase I. Phylogenetic analysis based on Maximum Likelihood indicated that all unknown specimens are conspecific with male imagines of S. multilabeculatus (bootstrap 100% and genetic distance 0–0.004). Male and female nymphs, female subimago and egg are described for the first time. Nymphs each bear a medial emargination on the hypopharynx, one bristle-like process at the base of the left prostheca, and a bifurcate rudimentary gill on abdominal segment VII. The male usually has smudges and light blotches on its forewings; the penis extends to the basal segment of the forceps and reaches to approximately 1/3 of the second segment of the forceps. Forewings of the female subimago have dark colour over more than half of the basal area, and the distal portion of each wing is translucent. The egg has a rounded pole; the polar cap covers approximately 1/4 of the surface; and the surface is covered with hexagonal structures. 

DNA-based association and description of the larval stage of Apatania theischingerorum Malicky 1981 (Trichoptera, Apataniidae), with notes on its ecology

Zootaxa ◽  
2018 ◽  
Vol 4418 (2) ◽  
pp. 161 ◽  
Author(s):  
JOHANN WARINGER ◽  
MARCOS A. GONZÁLEZ ◽  
LUIS MARTÍN ◽  
JESÚS MARTÍNEZ ◽  
FELICITAS ERZINGER ◽  
...  
Keyword(s):  
Larval Stage ◽  
Abdominal Segment ◽  
Sequence Data ◽  
Genetic Data ◽  
Mitochondrial Cytochrome ◽  
Species Pair ◽  
Diagnostic Features ◽  
Adult Females ◽  
Distribution Ranges ◽  
The Alps

The hitherto unknown larva of Apatania theischingerorum Malicky 1981 is described, based on the association with adult females using sequence data from the mitochondrial cytochrome c oxidase region. Genetic data confirmed the distinct status of this taxon within the parthenogenetic Apatania muliebris complex (Schmid 1954). We provide information on the morphology of the larva and figure the most important diagnostic features. Apatania theischingerorum is morphologically close to A. fimbriata (Pictet 1834). In the context of the Apataniidae key of Waringer et al. (2015), the species pair can be separated by pleural setation patterns on abdominal segment I and by their distribution ranges in Europe: A. fimbriata is known from the Alps, the western and central highlands, the western plains, the Hungarian lowlands and the Carpathians, whereas A. theischingerorum has been recorded exclusively on the Iberian peninsula.

RELIABLE SEXING OF ADULT CONOPHTHORUS (COLEOPTERA: SCOLYTIDAE) BEETLES

1995 ◽  
Vol 127 (4) ◽  
pp. 605-607 ◽  
Author(s):  
Jorge A. Santiago-Blay ◽  
Traivis L. Young
Keyword(s):  
Reliable Method ◽  
Abdominal Tergite ◽  
Head Width ◽  
Scanning Electron Micrographs ◽  
Adult Males ◽  
Diagnostic Features ◽  
Genital Opening ◽  
Males And Females ◽  
Non Destructive ◽  
Scanning Electron

Conophthorus Hopkins 1915 cone beetles are among the most damaging pests of pine (Pinus spp.) seed production in North America (Hedlin et al. 1980; Cibrián-Tovar et al. 1986). Current work on cone beetle molecular identification and semiochemicals requires a rapid and reliable method for sexing adult beetles. Head microsculpturation (Schwarz 1895; Wood 1982), overall beetle length, head width, and internal genital structures (or parts of them) visible when extruded through the genital opening (Lyons 1956) have been suggested as characters to sex adult Conophthorus. Herdy (1959) pointed out that abdominal tergite morphology could be used to distinguish males from females. The latter seems to be the most reliable, non-destructive way to sex Conophthorus specimens. Published illustrations (Kinzer and Ridgill 1972) have been found difficult to interpret by many researchers. To increase the reliability in sexing adult Conophthorus beetles, we prepared scanning electron micrographs (SEMs) of the terminal abdominal tergites of adult males and females, which we present here, clearly showing the diagnostic features of each sex.

scholarly journals A new hybrid from Taiwan, Elatostema ×hybrida (Urticaceae), is the first confirmed natural hybrid for Urticaceae

Phytotaxa ◽  
2014 ◽  
Vol 161 (1) ◽  
pp. 43 ◽  
Author(s):  
Yu-Hsin Tseng ◽  
Jer-Ming Hu
Keyword(s):  
Sequence Data ◽  
Pollen Viability ◽  
Pollen Dispersal ◽  
Distribution Patterns ◽  
Morphological Characters ◽  
Natural Hybridization ◽  
Putative Hybrid ◽  
Natural Hybrid ◽  
Hybrid Species ◽  
Dna Sequence Data

Explosive pollen dispersal is common in Urticaceae and they are thought to be wind-pollinated. Despite a lack of obvious mechanism for preventing cross-species pollination, putative hybrid species in Urticaceae are rarely documented. Here we described the first natural hybrid in Urticaceae Elatostema ×hybrida from Taiwan. Morphological characters in E. ×hybrida are intermediate between putative parental species: E. lineolatum var. majus and E. platyphylloides. Six hybrid populations of E. ×hybrida were found in Taiwan that exhibited largely overlapping distribution patterns with its putative parents. Phylogenetic analysis of chloroplast DNA showed that the hybrid species is more closely related to E. lineolatum var. majus suggesting that the latter is the maternal parent and that hybridization is unidirectional. The chromosome number of E. ×hybrida remains the same as its putative parents (2n = 26). We speculate that the examined hybrids are natural first-generation results of independent hybridization events. Based on the morphology, spatial distribution, DNA sequence data, pollen viability and cytological observations, we hypothesize that E. ×hybrida is derived from natural hybridization events between E. lineolatum var. majus (♀) and E. platyphylloides (♂).

scholarly journals Revision of Eudorylas Aczél, 1940 (Diptera, Pipunculidae) in the Middle East, with the description of four new species

2020 ◽  
Vol 8 ◽  
Author(s):  
Behnam Motamedinia ◽  
Jeffrey Skevington ◽  
Scott Kelso
Keyword(s):  
New Species ◽  
Middle East ◽  
Sequence Data ◽  
Middle Eastern ◽  
Additional Species ◽  
Mitochondrial Coi ◽  
Dna Sequence Data ◽  
Coi Barcoding ◽  
Males And Females ◽  
Taxonomic Approach

The Middle Eastern species of Eudorylas Aczél, 1940 are revised through an integrative taxonomic approach by combining morphological and sequence data from the mitochondrial COI barcoding gene. Four new species of the genus Eudorylas are described, males and females of three species are associated, DNA sequence data of 11 Middle Eastern Eudorylas species are provided and 15 additional species are discussed. To facilitate their recognition, we provide diagnoses, descriptions, an identification key and distributional maps for all species. The following new species are described from the Middle East: E. avis Motamedinia & Skevington sp. n., E. bihamatus Motamedinia & Skevington sp. n., E. corniculans Motamedinia & Skevington sp. n., E. nasicus Motamedinia & Skevington sp. n.

Morphological and molecular characterisation of a new genus and new species of Parabathynellidae (Crustacea : Syncarida) in Queensland, Australia

2017 ◽  
Vol 31 (2) ◽  
pp. 208 ◽  
Author(s):  
J. Little ◽  
A. I. Camacho
Keyword(s):  
New Genus ◽  
Nuclear Dna ◽  
Sequence Data ◽  
South Australia ◽  
Morphological Description ◽  
New Genus And Species ◽  
Distinctive Character ◽  
Dna Sequence Data ◽  
The Family ◽  
Males And Females

A new genus and species in the family Parabathynellidae Noodt, 1965, Lockyerenella danschmidti, gen. et sp. nov., is described from Queensland, Australia. The species displays several distinctive character states within the Parabathynellidae, and a unique combination of character states. Interestingly, L. danschmidti is the only known species of Parabathynellidae in Australia (and in the world) to possess a long and robust seta on the last segment of the antenna, which is present in both males and females. The new genus is morphologically distinct from the known genera, but is more similar to Queensland species of Notobathynella Schminke, 1973 and Arkaroolabathynella Abrams & King, 2013 from South Australia, than to species from other areas of the continent. This is the first work to couple genetics to the morphological description of a stygofauna species in Queensland. Phylogenetic relationships between L. danschmidti and related genera from elsewhere on the continent were evaluated through the analysis of combined mitochondrial and nuclear DNA sequence data. This analysis revealed at least one new genus within the Parabathynellidae, confirming our morphological hypothesis.

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