Three new Nearctic genera of oak cynipid gall wasps (Hymenoptera: Cynipidae: Cynipini): Burnettweldia Pujade-Villar, Melika & Nicholls, Nichollsiella Melika, Pujade-Villar & Stone, Disholandricus Melika, Pujade-Villar & Nicholls; and re-establishment of the genus Paracraspis Weld

Zootaxa ◽  
2021 ◽  
Vol 4993 (1) ◽  
pp. 1-81
Author(s):  
GEORGE MELIKA ◽  
JULI PUJADE-VILLAR ◽  
JAMES A. NICHOLLS ◽  
VICTOR CUESTA-PORTA ◽  
CRYSTAL COOKE-McEWEN ◽  
...  
Keyword(s):  
Dna Sequences ◽  
New Genera ◽  
Gall Wasps

We describe three new genera of cynipid oak gall wasps from the Nearctic: Burnettweldia Pujade-Villar, Melika & Nicholls, gen. nov., Nichollsiella Melika, Pujade-Villar & Stone, gen. nov., and Disholandricus Melika, Pujade-Villar & Nicholls, gen. nov. (Hymenoptera: Cynipidae: Cynipini). Burnettweldia includes five species, B. californicordazi Cuesta-Porta, Melika & Pujade-Villar, sp. nov., B. conalis (Weld), comb. nov., B. corallina (Bassett), comb. nov., B. plumbella (Kinsey), comb. nov., B. washingtonensis (Gillette), comb. nov.. Nichollsiella includes three species, N. arizonica (Cockerell), comb. nov., N. sulcata (Ashmead), comb. nov., and N. puigi Melika, Cuesta-Porta & Pujade-Villar, sp. nov.. Disholandricus includes four species, D. chrysolepidis (Ashmead), comb. nov., D. lasius (Ashmead), comb. nov., D. reniformis (McCracken & Egbert), comb. nov., D. truckeensis (Ashmead), comb. nov. The genus Paracraspis Weld, comb. rev. is re-established with three species, P. guadaloupensis (Fullaway), P. insolens (Weld), and P. patelloides (Trotter). Descriptions, re-descriptions, diagnoses, keys to genera and species are given, including data on DNA sequences, biology, phenology and distribution.  

scholarly journals The Callerya Group redefined and Tribe Wisterieae (Fabaceae) emended based on morphology and data from nuclear and chloroplast DNA sequences

PhytoKeys ◽  
2019 ◽  
Vol 125 ◽  
pp. 1-112 ◽  
Author(s):  
James A. Compton ◽  
Brian D. Schrire ◽  
Kálmán Könyves ◽  
Félix Forest ◽  
Panagiota Malakasi ◽  
...  
Keyword(s):  
East Asia ◽  
Chloroplast Dna ◽  
Dna Sequences ◽  
New Combination ◽  
New Combinations ◽  
New Genera ◽  
Infraspecific Taxa ◽  
Nuclear Its ◽  
Single Genus ◽  
Chloroplast Dna Sequences

The Tribe Wisterieae (Zhu 1994), founded on the single genus Wisteria, is emended and recircumscribed based on morphology and data from nuclear ITS and ndhJ-trnF, matK and rbcL chloroplast DNA sequences. This newly enlarged tribe comprises 36 species and 9 infraspecific taxa within 13 described genera. Six genera are new, two are reinstated and five were previously placed in Tribe Millettieae. The genus Adinobotrys is also reinstated comprising two species including the new combination A.vastus. Other reinstated genera include Whitfordiodendron, with four species, and Padbruggea, with three species, including the reinstatement of P.filipes and the new combination P.filipesvar.tomentosa. The existing genera Afgekia, Callerya, Endosamara (with the new combination E.racemosavar.pallida), Sarcodum and Wisteria, with the new combinations W.frutescenssubsp.macrostachya are evaluated. The new genera comprise three Australasian species in Austrocallerya: A.australis, A.megasperma and A.pilipes; Wisteriopsis with five species from east Asia has six new combinations: W.japonica, W.kiangsiensis, W.championii, W.eurybotrya, W.reticulata and W.reticulatavar.stenophylla. Two species comprise the new Thai genus Kanburia: K.tenasserimensis and K.chlorantha. Nanhaia comprises the two species: N.fordii and N.speciosa and the monotypic genera Sigmoidala and Serawaia are based respectively on the species S.kityana and S.strobilifera. Lectotypes are designated for the names Adinobotrysfilipes, A.myrianthus, Millettiabonatiana, Millettiabracteosa, Millettiachampionii, Millettiacinerea, Millettiadielsiana, Millettiakityana, M.maingayi, Millettianitida, Millettiaoocarpa, Millettiapurpurea, M.reticulata, M.reticulatavar.stenophylla, Padbruggeadasyphylla, Pterocarpusaustralis, Robiniaracemosa, Whitfordiodendronscandens, W.sumatranum and Wisteriapallida. A neotype is designated for the name Millettialeiogyna.

scholarly journals Taxonomy and phylogeny of Postia.Multi-gene phylogeny and taxonomy of the brown-rot fungi: Postia (Polyporales, Basidiomycota) and related genera

2019 ◽  
Vol 42 (1) ◽  
pp. 101-126 ◽  
Author(s):  
L.L. Shen ◽  
M. Wang ◽  
J.L. Zhou ◽  
J.H. Xing ◽  
B.K. Cui ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Brown Rot ◽  
Small Subunit ◽  
Rrna Gene ◽  
New Genera ◽  
Brown Rot Fungi

Phylogenetic and taxonomic studies on the brown-rot fungi Postia and related genera, are carried out. Phylogenies of these fungi are reconstructed with multiple loci DNA sequences including the internal transcribed spacer regions (ITS), the large subunit (nLSU) and the small subunit (nSSU) of nuclear ribosomal RNA gene, the small subunit of mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α gene (TEF1), the largest subunit of RNA polymerase II (RPB1) and the second subunit of RNA polymerase II (RPB2). Ten distinct clades of Postia s.lat. are recognized. Four new genera, Amaropostia, Calcipostia, Cystidiopostia and Fuscopostia, are established, and nine new species, Amaropostia hainanensis, Cyanosporus fusiformis, C. microporus, C. mongolicus, C. piceicola, C. subhirsutus, C. tricolor, C. ungulatus and Postia sublowei, are identified. Illustrated descriptions of the new genera and species are presented. Identification keys to Postia and related genera, as well as keys to the species of each genus, are provided.

scholarly journals A new combination in Mcneillia (Caryophyllaceae) for the Italian flora

Phytotaxa ◽  
2014 ◽  
Vol 170 (2) ◽  
pp. 139 ◽  
Author(s):  
FABRIZIO BARTOLUCCI ◽  
FABIO CONTI ◽  
DUILIO IAMONICO ◽  
E. DEL GUACCHIO
Keyword(s):  
Dna Sequences ◽  
Morphological Characters ◽  
New Combination ◽  
New Genera ◽  
Italian Flora

On the basis of DNA sequences of nrITS and plastid matK from 160 ingroup and 105 outgroup samples, Dillenberger & Kadereit (2014) reconstructed the phylogeny and the evolution of the relevant morphological characters of the genus Minuartia Linnaeus (1753: 89), to show that this genus is highly polyphyletic. A revised circumscription of Minuartia was proposed with the recognition of Cherleria Linnaeus (1753: 425), Eremogone Fenzl (1833: 13), Facchinia Reichenbach (1841: 63), Mononeuria Reichenbach (1841: 118), Rhodalsine Gay (1845: 25), Sabulina Reichenbach (1832: 24) and Triplateia Bartling (1830: 305), and recognition of new genera Mcneillia Dillenberger & Kadereit (2014: 83), Minuartiella Dillenberger & Kadereit (2014: 84) and Pseudocherleria Dillenberger & Kadereit (2014: 84).

Two new genera of Oriental xyleborine ambrosia beetles (Coleoptera, Curculionidae: Scolytinae)

Zootaxa ◽  
2020 ◽  
Vol 4722 (6) ◽  
pp. 540-554 ◽  
Author(s):  
ANTHONY I. COGNATO ◽  
SARAH M. SMITH ◽  
ROGER A. BEAVER
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Dna Sequences ◽  
Distinct Species ◽  
Sequence Difference ◽  
New Combinations ◽  
New Genera ◽  
Diagnostic Characters ◽  
Species Groups ◽  
Three New Species

As part of an ongoing revision of the Southeast Asian fauna two distinct species groups were identified and hypothesized as new genera. These species groups were monophyletic as evidenced by a Bayesian analysis of DNA sequences from four genes for 181 xyleborine taxa augmented by 18 species newly included in this phylogenetic analysis. The species groups and newly discovered species demonstrated unique combinations of diagnostic characters and levels of DNA sequence difference commensurable to other xyleborine taxa. Hence, two new genera and three new species were described: Fraudatrix gen. n., Tricosa gen. n., Tricosa cattienensis sp. n., T. indochinensis sp. n., T. jacula sp. n.. The following new combinations are proposed: Fraudatrix cuneiformis (Schedl, 1958) (Xyleborus) comb. n., Fraudatrix melas (Eggers, 1927) comb. n., F. pileatula (Schedl, 1975) (Xyleborus) comb. n., F. simplex (Browne, 1949), (Cryptoxyleborus) comb. n., Tricosa mangoensis (Schedl, 1942) (Xyleborus) comb. n., T. metacuneola (Eggers, 1940) (Xyleborus) comb. n. Keys to the females of the species included in the new genera are presented. Diagnostic characters are given for the genera and species, and the distribution and biology of each taxon is discussed. The addition of these new genera increases the number of recognized genera of Xyleborini to 41. 

Speciation history of three closely related oak gall wasps,Andricus mukaigawae,A. kashiwaphilus, andA. pseudoflos(Hymenoptera: Cynipidae) inferred from nuclear and mitochondrial DNA sequences

2012 ◽  
Vol 21 (19) ◽  
pp. 4681-4694 ◽  
Author(s):  
Nakatada Wachi ◽  
Yoshihisa Abe ◽  
Nobuyuki Inomata ◽  
Alfred Edward Szmidt ◽  
Hidenori Tachida
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Gall Wasps ◽  
History Of

Systematics of the Gracilariales (Rhodophyta) including new subfamilies, tribes, subgenera, and two new genera, Agarophyton gen. nov. and Crassa gen. nov.

Phytotaxa ◽  
2018 ◽  
Vol 374 (1) ◽  
pp. 1 ◽  
Author(s):  
CARLOS FREDERICO D. GURGEL ◽  
JAMES N. NORRIS ◽  
WILLIAM E. SCHMIDT ◽  
HAU NHU LE ◽  
SUZANNE FREDERICQ
Keyword(s):  
Dna Sequences ◽  
Evolutionary History ◽  
New Genera ◽  
New Classification ◽  
Free Living ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
History Of ◽  
Evolutionary Lineages ◽  
Stable Classification

The Gracilariales is a red macroalgal order and the main global source of the economically important agar, a marine phycocolloid. Independent comparative morphological and molecular phylogenetic studies over the last 20 years have revealed the existence of seven major clades recognizable as distinct genera. Of these major clades only four free-living genera have been widely accepted taxonomically: Curdiea, Melanthalia, Gracilariopsis, and Gracilaria. Three other clades comprise the reinstatement of the genus Hydropuntia and the proposal of two new genera, Agarophyton and Crassa, described herein. Based on new rbcL DNA sequences, and along with a reassessment of published comparative morphological and molecular phylogenetic studies, we argue that the latter three genera represent distinct evolutionary lineages in the Gracilariaceae, and propose a new classification for the order Gracilariales. Our new proposal incorporates the most current understanding of the evolutionary history of the order, establishes a natural and stable classification system, and provides the basis for the recognization of intra-family ranks. Our classification scheme reconciles all molecular phylogenetic studies published to date.

Unity in diversity: phylogenetics and taxonomy of Rhabdoweisiaceae (Dicranales, Bryophyta)

2020 ◽  
Author(s):  
Vladimir E Fedosov ◽  
Alina V Fedorova ◽  
Juan Larraín ◽  
Marina B Santos ◽  
Michael Stech ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Divergent Evolution ◽  
New Genera ◽  
Morphological Heterogeneity ◽  
Phylogenetic Reconstructions ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Unity In Diversity

Abstract Molecular phylogenetic reconstructions of Rhabdoweisiaceae based on plastid (trnS-rps4 and trnL-F) and mitochondrial (nad5) DNA sequences and a comprehensive taxon sampling were performed, including 15 out of 16 genera currently attributed to the family, taxa recently found belonging to Rhabdoweisiaceae and expanded outgroups from related families of Dicranidae. The suggested revised delimitation of the family comprises Camptodontium (previously referred to Dicranaceae), three subaquatic species currently classified in Blindia (Seligeriaceae), and Glyphomitrium (previously referred to Ptychomitriaceae, Erpodiaceae or its own family) and Eucamptodon perichaetialis (the type species of the genus, previously referred to Dicnemonaceae). The emended delimitation of Rhabdoweisiaceae thus adds to the already high morphological heterogeneity resulting from the divergent evolution of specialized traits in different lineages of epiphytes and saxicolous hygrophytes. Taxonomic inferences from the phylogenetic analyses include segregation of three new genera, Pseudoblindia (for species currently recognized as Kiaeria falcata, Blindia robusta, B. lewinskyae and B. inundata), Ripariella (for Kiaeria riparia) and Brideliella (for Oncophorus wahlenbergii, O. demetrii and Symblepharis lindigii). Hollia is reinstated for the dicnemonoid Australasian species previously classified in Eucamptodon. Additionally, we propose to merge (1) Oreoweisia, Pocsiella and Pseudohyophila with Cynodontium, and (2) Kiaeria s.s. with Arctoa. Furthermore, we propose to limit Oncophorus to O. virens and O. integerrimus and expand the circumscription of Symblepharis to accommodate O. crispifolius, O. dendrophilus, O. elongatus and O. raui. The generic status and inclusion in Rhabdoweisiaceae are confirmed for Cnestrum and the recently described Notocynodontium, and the exclusion of Amphidium, Dichodontium, Holodontium and Hymenoloma from the family is supported.

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

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