scholarly journals Impact of Biofield Energy Healing Treated Vitamin D<sub>3</sub> on Human Osteoblast Cell Line (MG-63) for Bone Health

2018 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
William Dean Plikerd
Keyword(s):  
Vitamin D ◽  
Cell Line ◽  
Bone Health ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Osteoblast Cell Line ◽  
Energy Healing

Activation of JNK and PAK2 is essential for citrinin-induced apoptosis in a human osteoblast cell line

2009 ◽  
Vol 24 (4) ◽  
pp. 343-356 ◽  
Author(s):  
Yu-Ting Huang ◽  
Ching-Yu Lai ◽  
Shyh-Liang Lou ◽  
Jui-Ming Yeh ◽  
Wen-Hsiung Chan
Keyword(s):  
Cell Line ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Osteoblast Cell Line ◽  
Induced Apoptosis

The Role of Nitric Oxide on the Proliferation of a Human Osteoblast Cell Line Stimulated With Hydroxyapatite

2008 ◽  
Vol 34 (4) ◽  
pp. 196-202 ◽  
Author(s):  
Wihaskoro Sosroseno ◽  
Erwan Sugiatno ◽  
Abdul Rani Samsudin ◽  
Mohd Fikri Ibrahim
Keyword(s):  
Nitric Oxide ◽  
Cell Proliferation ◽  
Cell Line ◽  
No Production ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Integrin Αv ◽  
Osteoblast Cell Line ◽  
Nitrite Production

Abstract The aim of the present study was to test the hypothesis that the proliferation of a human osteoblast cell line (HOS cells) stimulated with hydroxyapatite (HA) may be regulated by nitric oxide (NO). The cells were cultured on the surface of HA. Medium or cells alone were used as controls. L-arginine, D-arginine, 7-NI (an nNOS inhibitor), L-NIL (an iNOS inhibitor), L-NIO (an eNOS inhibitor) or carboxy PTIO, a NO scavenger, was added in the HA-exposed cell cultures. The cells were also precoated with anti-human integrin αV antibody. The levels of nitrite were determined spectrophotometrically. Cell proliferation was assessed by colorimetric assay. The results showed increased nitrite production and cell proliferation by HA-stimulated HOS cells up to day 3 of cultures. Anti-integrin αV antibody, L-NIO, or carboxy PTIO suppressed, but L-arginine enhanced, nitrite production and cell proliferation of HA-stimulated HOS cells. The results of the present study suggest, therefore, that interaction between HA and HOS cell surface integrin αV molecule may activate eNOS to catalyze NO production which, in turn, may regulate the cell proliferation in an autocrine fashion.

scholarly journals The Effects of Polyphenol, Tannic Acid, or Tannic Acid in Combination with Pamidronate on Human Osteoblast Cell Line Metabolism

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 451
Author(s):  
Hermizi Hapidin ◽  
Nor Munira Hashim ◽  
Mohamad Zahid Kasiram ◽  
Hasmah Abdullah
Keyword(s):  
Cell Line ◽  
Tannic Acid ◽  
Treated Group ◽  
Histochemical Staining ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Osteoblast Cell Line ◽  
Combination Treated Group ◽  
Index Value

Background: This study investigates the effect of tannic acid (TA) combined with pamidronate (PAM) on a human osteoblast cell line. Methods: EC50 for TA, PAM, and different combination ratios of TA and PAM (25:75, 50:50, 75:25) were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The combination index value was utilized to analyze the degree of drug interaction, while trypan blue assay was applied to analyze the cells proliferation effect. The mineralization and detection of bone BSP and Osx genes were determined via histochemical staining and PCR test, respectively. Results: The EC50 of osteoblasts treated with TA and a 75:25 ratio of TA and PAM were more potent with lower EC50 at 0.56 µg/mL and 0.48 µg/mL, respectively. The combination of TA and PAM (75:25) was shown to have synergistic interaction. On Day 7, both TA and PAM groups showed significantly increased proliferation compared with control and combination groups. On Day 7, both the TA and combination-treated groups demonstrated a higher production of calcium deposits than the control and PAM-treated groups. Moreover, on Day 7, the combination-treated group showed a significantly higher expression of BSP and Osx genes than both the TA and PAM groups. Conclusion: Combination treatment of TA and PAM at 75:25 ameliorated the highest enhancement of osteoblast proliferation and mineralization as well as caused a high expression of BSP and Osx genes.

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