An Avirulent Ralstonia Solanacearum Strain Undergoes Phenotype Conversion from a Pathogenic Strain Under Natural Environment

ABSTRACT Moko is one of the main diseases affecting banana and plantain in Colombia. Here, we report the genome sequence of the causal agent, the bacterium Ralstonia solanacearum (Smith) strain CIAT-078, collected in 2004 from affected plantains in central-west Colombia. The assembled genome was obtained using Oxford Nanopore Technology.

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Complete Genome Sequence of the Plant Pathogen Ralstonia solanacearum Strain Po82

Journal of Bacteriology ◽

10.1128/jb.05384-11 ◽

2011 ◽

Vol 193 (16) ◽

pp. 4261-4262 ◽

Cited By ~ 36

Author(s):

J. Xu ◽

H.-j. Zheng ◽

L. Liu ◽

Z.-c. Pan ◽

P. Prior ◽

...

Keyword(s):

Genome Sequence ◽

Plant Pathogen ◽

Ralstonia Solanacearum ◽

Complete Genome Sequence ◽

Complete Genome ◽

Solanacearum Strain

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Contribution of a lectin, LecM, to the quorum sensing signalling pathway of Ralstonia solanacearum strain OE1-1

Molecular Plant Pathology ◽

10.1111/mpp.12757 ◽

2018 ◽

Vol 20 (3) ◽

pp. 334-345 ◽

Cited By ~ 5

Author(s):

Kazusa Hayashi ◽

Kenji Kai ◽

Yuka Mori ◽

Shiho Ishikawa ◽

Yumeto Ujita ◽

...

Keyword(s):

Quorum Sensing ◽

Ralstonia Solanacearum ◽

Signalling Pathway ◽

Solanacearum Strain

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Draft Genome Sequence of Ralstonia solanacearum Strain Rs-T02, Which Represents the Most Prevalent Phylotype in Guangxi, China

Genome Announcements ◽

10.1128/genomea.00241-16 ◽

2016 ◽

Vol 4 (2) ◽

Cited By ~ 2

Author(s):

Chengwu Zou ◽

Kaihao Wang ◽

Jiaorong Meng ◽

Gaoqing Yuan ◽

Wei Lin ◽

...

Keyword(s):

Genome Sequence ◽

Ralstonia Solanacearum ◽

Draft Genome ◽

Draft Genome Sequence ◽

Comparative Genomic ◽

Sequence Information ◽

Guangxi Province ◽

Solanacearum Strain ◽

Genomic Studies ◽

Genome Sequence Information

Ralstonia solanacearum strain Rs-T02 was originally isolated from a bacterial wilt of tomato plant in Nanning City of Guangxi Province, China. It represents the most prevalent phylotype in Guangxi. Here, we present the draft genome sequence of this strain, which comprises 5,225 genes and 5,976,011 nucleotides with an average G+C content of 66.79%. There are 968 different genes between this isolate and the previously reported genome sequence of Ralstonia solanacearum GMl l000 (race l, biovar 3, phylotype I), and the genome sequence information of this isolate may be useful for comparative genomic studies to determine the genetic diversity in this species.

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A Ralstonia solanacearum Strain from Guatemala Infects Diverse Flower Crops, Including New Asymptomatic Hosts Vinca and Sutera, and Causes Symptoms in Geranium, Mandevilla Vine, and New Host African Daisy (Osteospermum ecklonis)

Plant Health Progress ◽

10.1094/php-rs-16-0001 ◽

2016 ◽

Vol 17 (2) ◽

pp. 114-121 ◽

Cited By ~ 11

Author(s):

Jordan Weibel ◽

Tuan Minh Tran ◽

Ana Maria Bocsanczy ◽

Margery Daughtrey ◽

David J. Norman ◽

...

Keyword(s):

Ralstonia Solanacearum ◽

Ornamental Plants ◽

New Host ◽

Solanacearum Strain ◽

Latently Infected ◽

Production Temperature ◽

The Common ◽

Highly Virulent ◽

Ornamental Species ◽

Agent Group

A strain of Ralstonia solanacearum was associated with wilting and blight of flowering ornamentals grown from cuttings that originated in Guatemala. This isolate, UW757, could wilt geranium, Osteospermum spp., and mandevilla, but was more aggressively virulent on tomato. Although it rarely caused symptoms, after inoculation UW757 colonized the stems of 11 widely grown ornamental species, reaching mean populations as high as 109 CFU/g stem at the common tropical highland production temperature of 24°C. UW757 belongs to phylotype I, sequevar 14 of the R. solanacearum species complex, not to the Race 3 biovar 2 Select Agent group that also infects geraniums. Whole genome sequencing revealed that UW757 is closely related to several Asian R. solanacearum strains and also to P781, a mandevilla-infecting strain established in Florida. Strains similar to UW757 were isolated from tomato in Guatemala in 2006 and 2015, indicating that this group is common and persistent. This finding enlarges the known host range of R. solanacearum, which was not previously known to infect Osteospermum, Vinca, or Sutera spp. Growers should be aware that a broad range of ornamental plants, including previously unknown hosts, can be latently infected by non-Race 3 biovar 2 strains of R. solanacearum that are also highly virulent on tomato. Accepted for publication 28 April 2016. Published 26 May 2016.

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Xylem Colonization by an HrcV¯ Mutant of Ralstonia solanacearum Is a Key Factor for the Efficient Biological Control of Tomato Bacterial Wilt

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.1998.11.9.869 ◽

1998 ◽

Vol 11 (9) ◽

pp. 869-877 ◽

Cited By ~ 26

Author(s):

Christophe Etchebar ◽

Danièle Trigalet-Demery ◽

Frédérique van Gijsegem ◽

Jacques Vasse ◽

André Trigalet

Keyword(s):

Mutant Strain ◽

Ralstonia Solanacearum ◽

Lateral Root ◽

Pathogenic Strain ◽

Staining Procedure ◽

Bacterial Suspension ◽

Marker Genes ◽

Wild Type ◽

Root Tips ◽

Challenge Inoculation

Microscopic studies of the colonization of the vascular tissues of tomato by an HrcV¯ (formerly HrpO¯) mutant strain of Ralstonia solanacearum were carried out after either root inoculation of the mutant strain alone or delayed challenge inoculation by a pathogenic strain. The use of two different marker genes, lacZ and uidA, introduced into either mutant or wild-type strains, respectively, permitted histological observation for the presence of both strains simultaneously. In roots, both strains could be found together in infected root tips and in lateral root emergence sites (lateral root cracks), but these bacterial strains subsequently invaded separate xylem vessels in the root system. At the hypocotyl level, a novel staining procedure, in conjunction with bacterial isolation and counting, showed three vascular colonization patterns: exclusive colonization by each of the competitors or simultaneous presence of each strain in separate xylem vessels. The relative frequencies of these patterns depended upon the root inoculation techniques used. The presence of one population always influenced the density of the other challenge-inoculated population. In plants inoculated with both wild-type and mutant strains, the population of the wild-type strain is lower than in plants inoculated with the wild type alone. In contrast, growth of the HrcV¯ mutant strain was significantly increased in the presence of the pathogenic strain. Two agriculturally acceptable techniques for plant inoculation were tested. Inoculation of plants by transplanting them into soil amended with clay micro-granules impregnated with the HrcV¯ mutant strain gave higher and more reproducible colonization of the plants than inoculation by watering a bacterial suspension on the roots. Significant percentages of exclusive colonization by the HrcV¯ mutant strain were only obtained after the clay microgranule inoculation technique. Competition for space in xylem vessels is one of the possible explanations for the protective ability of the HrcV¯ mutant strain against subsequent invasion by a pathogenic strain.

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A Single Locus Leads to Resistance of Arabidopsis thaliana to Bacterial Wilt Caused by Ralstonia solanacearum Through a Hypersensitive-like Response

Phytopathology ◽

10.1094/phyto.1999.89.8.673 ◽

1999 ◽

Vol 89 (8) ◽

pp. 673-678 ◽

Cited By ~ 11

Author(s):

Gan-Der Ho ◽

Chang-Hsien Yang

Keyword(s):

Arabidopsis Thaliana ◽

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Northern Blot Analysis ◽

Plant Diseases ◽

Glutathione S Transferase ◽

Solanacearum Strain ◽

Whole Plant ◽

Pathogenesis Related Protein ◽

Pathogenesis Related

Strains of Ralstonia solanacearum have been shown to cause bacterial wilt in some, but not all, ecotypes of Arabidopsis thaliana. We demonstrate here that after inoculation of the leaves of resistant ecotype S96 with R. solanacearum strain Ps95 necrosis around the inoculation site rapidly appeared and no further symptoms developed in the plant. Leaves of susceptible ecotype N913 completely wilted 7 days after inoculation with Ps95, and symptoms spread systemically throughout the whole plant within 2 weeks after inoculation. These results suggest that the resistance of Arabidopsis S96 to R. solanacearum is due to a response similar to the hypersensitive response (HR) observed in other plant diseases. Northern blot analysis of the expression of defense-related genes, known to be differentially induced during the HR in Arabidopsis, indicated that pathogenesis-related protein PR-1, glutathione S-transferase (GST1), and Cu, Zn superoxide dismutase (SOD) mRNAs increased significantly in S96 leaves between 3 to 12 h after infiltration with Ps95. The induction of these genes in susceptible ecotype N913 by Ps95 was clearly delayed. Genetic analysis of crosses between resistant ecotype S96 and susceptible ecotype N913 indicated that resistance to Ps95 is due to a single dominant locus.

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