Evaluation of Pathogenic Big 7 E. coli Aptamer-Quantum Dot Lateral Flow Test Strips

AbstractTest strips are convenient tools for rapid, semi-quantitative analysis of a variety of parameters by dipping them for a few seconds in a sample solution followed by a simple colorimetric read-out. Their sensitivity is mainly determined by the reactivity of the test dyes on the reaction zone and is not sufficient for some applications. The detection limit of commercially available free chlorine test strips, for example, is at present not low enough to confirm the absence of this analyte as disinfectant in rinsing solutions after disinfection or to control required residual amounts of chlorine in drinking water. Therefore, we developed a user-friendly lateral flow test which is capable to detect very low amounts of free chlorine. The latter relies on a larger sample volume passing the reaction zone as compared to simple dip test strips. An amount of as low as 0.05 ppm chlorine can, however, only be detected if oxidation stable flow test substrates are used. The eventually developed flow test reaches a 10x higher sensitivity than a commercial dip test. The result is obtained within 4–5 min flow time, whereby no action is required by the user during this analysis time.

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Multiplex Lateral-Flow Test Strips Fabricated by Two-Dimensional Shaping

ACS Applied Materials & Interfaces ◽

10.1021/am800043z ◽

2008 ◽

Vol 1 (1) ◽

pp. 124-129 ◽

Cited By ~ 306

Author(s):

Erin M. Fenton ◽

Monica R. Mascarenas ◽

Gabriel P. López ◽

Scott S. Sibbett

Keyword(s):

Lateral Flow ◽

Two Dimensional ◽

Test Strips ◽

Flow Test ◽

Lateral Flow Test

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Integrated Graphene Oxide Purification-Lateral Flow Test Strips (iGOP-LFTS) for Direct Detection of PCR Products with Enhanced Sensitivity and Specificity

Analytical Chemistry ◽

10.1021/acs.analchem.7b02769 ◽

2017 ◽

Vol 89 (22) ◽

pp. 12137-12144 ◽

Cited By ~ 11

Author(s):

Shanglin Li ◽

Yin Gu ◽

Yi Lyu ◽

Yan Jiang ◽

Peng Liu

Keyword(s):

Graphene Oxide ◽

Sensitivity And Specificity ◽

Direct Detection ◽

Lateral Flow ◽

Test Strips ◽

Enhanced Sensitivity ◽

Flow Test ◽

Pcr Products ◽

Lateral Flow Test

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A sensitive lateral flow test strip based on silica nanoparticle/CdTe quantum dot composite reporter probes

RSC Advances ◽

10.1039/c2ra00976e ◽

2012 ◽

Vol 2 (5) ◽

pp. 1778 ◽

Cited By ~ 34

Author(s):

Yalong Bai ◽

Chunyuan Tian ◽

Xinlin Wei ◽

Yuanfeng Wang ◽

Dapeng Wang ◽

...

Keyword(s):

Quantum Dot ◽

Silica Nanoparticle ◽

Test Strip ◽

Lateral Flow ◽

Flow Test ◽

Lateral Flow Test ◽

Cdte Quantum Dot

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Modification of a nitrocellulose membrane with nanofibers for sensitivity enhancement in lateral flow test strips

RSC Advances ◽

10.1039/d1ra04369b ◽

2021 ◽

Vol 11 (43) ◽

pp. 26493-26501

Author(s):

Xue Wang ◽

Chao-Hua Xue ◽

Dong Yang ◽

Shun-Tian Jia ◽

Ya-Ru Ding ◽

...

Keyword(s):

Detection Limit ◽

Sensitivity Enhancement ◽

Lateral Flow ◽

Fibrous Membrane ◽

Nitrocellulose Membrane ◽

Test Strips ◽

Flow Test ◽

New Type ◽

Lateral Flow Test

We constructed a new type of ICT strip by replacing the conventional nitrocellulose membrane with an electrospin-coated nitrocellulose (ENC) fibrous membrane, and the ICT strip could obtain an HCG detection limit of 0.22 mIU mL−1, and an LH detection limit of 0.36 mIU mL−1.

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