Effect of Silencing Information Regulator on Rat Cerebral Venous Thrombosis Through Regulating Vascular Endothelial Growth Factor

2019 ◽  
Vol 9 (11) ◽  
pp. 1607-1613
Author(s):  
Yan-Qing Liu ◽  
Yang Zhao ◽  
Yi-Jun Wu ◽  
Bo-Feng Liu ◽  
Hui-Ping Zhang ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Water Content ◽  
Real Time ◽  
Brain Tissue ◽  
Neurological Deficit ◽  
Real Time Pcr ◽  
Cerebral Venous Thrombosis ◽  
Vegf Expression ◽  
Tissue Water ◽  
Sirt1 Expression

Cerebral venous thrombosis (CVST) can cause severe dysfunction and even death. Silencing information regulator (SIRT1) involves in neurodegenerative diseases. However, whether SIRT1 participates in CVST is unclear. SD rats were divided into 3 groups, control group; CVST group and SIRT1 group (transfected with AAV-SIRT1 plasmid) followed by analysis of brain tissue SIRT1 and VEGF expression by Real time PCR, neurological deficit scores and brain tissue water content. Brain vascular endothelial cells (bVECs) were cultured and divided into NC group, SIRT1 group and si-SIRT1 group followed by analysis of cell proliferation by MTT assay, Caspase 3 activity, SIRT1 expression by Real time PCR and Western blot and VEGF expression and secretion by Real time PCR and ELISA. SIRT1 expression was decreased and VEGF expression was increased, along with increased score of neurological deficit and water content of brain tissue in CVST rats. Transfection of AAV-SIRT1 plasmid up-regulated SIRT1 expression in CVST rats, inhibited VEGF expression, improved neurological deficit score and brain tissue water content (P < 0.05). Overexpression of SIRT1 in bVECs significantly decreased cell proliferation, elevated Caspase 3 activity, and decreased VEGF expression and secretion, compared to NC group (P < 0.05). Knockdown of SIRT1 expression in bVECs reversed the above changes (P < 0.05). SIRT1 expression is decreased in CVST rats and up-regulation of SIRT1 can inhibit VEGF expression and improve neurological function. SIRT1 can inhibit the proliferation of bVECs and regulate cerebral venous thrombosis by regulating VEGF.

scholarly journals Assessment of the synergic effect of Avastin and VEGF siRNA on inhibition of cell proliferation and angiogenic factor expression of human breast cancer cells

2021 ◽  
Author(s):  
Abdolkhalegh Deezagi ◽  
Bahar Ghorbani
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cell Death ◽  
Real Time ◽  
Real Time Pcr ◽  
Angiogenic Factor ◽  
Cell Counting ◽  
Synergic Effect ◽  
Vegf Expression ◽  
Cell Growth And Migration

Abstract Angiogenesis is an important process in tumor growth and metastasis and vascular endothelial growth factor (VEGF) plays an important role in this process. Several VEGF inhibitors have been developed as anticancer agents including humanized monoclonal antibodies, and various small molecules. The aim of this work was to investigate the effect of combination of VEGF siRNA and Avastin on breast cancer MCF-7 cell line behavior. For this purpose, the cells were treated with different concentrations of Avastin and/or VEGF siRNA and their combination. The cell survival and cell proliferation were assayed by cell counting, trypan blue and MTT tests. The cell migration was assayed by scratching test. VEGF expression was assayed by RT and real-time PCR and ELISA methods. Results indicated the significant increase in cell death following treatment with Avastin (50% cell death at 100 µg/ml). Cell death with VEGF siRNA transfection was lower than Avastin, however, it was significant. This result in VEGF siRNA + Avastin (100 µg/ml) treatment was greater compared to treatment with each of these compounds alone (47%). Scratching results also showed the synergic effect of VEGF siRNA and Avastin (57% decrease). Real-time PCR results showed that Avastin at concentrations of ≥ 50 µg/ml led to 2.5 to 7.5-fold decrease in VEGF expression levels. Also, treatment with VEGF siRNA led to 15.5-fold decrease in VEGF expression. Finally, VEGF expression following VEGF siRNA + Avastin treatment led to a significant 47.5-fold decrease in VEGF expression. It could be concluded that combination of VEGF siRNA and Avastin have a more significant impact on the inhibition of cell growth and migration and it can probably be used as an effective therapeutic approach.

Detection of Polyoma Virus in Brain Tissue of Patients With Progressive Multifocal Leukoencephalopathy by Real-Time PCR and Pyrosequencing

2004 ◽  
Vol 13 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Rose C. Beck ◽  
Debra J. Kohn ◽  
Marion J. Tuohy ◽  
Richard A. Prayson ◽  
Belinda Yen-Lieberman ◽  
...  
Keyword(s):  
Progressive Multifocal Leukoencephalopathy ◽  
Real Time ◽  
Brain Tissue ◽  
Real Time Pcr ◽  
Polyoma Virus

scholarly journals Parthenolide Is Neuroprotective in Rat Experimental Stroke Model: Downregulating NF-κB, Phospho-p38MAPK, and Caspase-1 and Ameliorating BBB Permeability

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Lipeng Dong ◽  
Huimin Qiao ◽  
Xiangjian Zhang ◽  
Xiaolin Zhang ◽  
Chaohui Wang ◽  
...  
Keyword(s):  
Western Blot ◽  
Water Content ◽  
Brain Tissue ◽  
Neurological Deficit ◽  
Infarct Volume ◽  
Brain Water Content ◽  
Ischemic Brain ◽  
Caspase 1 ◽  
Ischemic Brain Tissue ◽  
Brain Water

Inflammatory damage plays an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Parthenolide (PN) has been proved to elicit a wide range of biological activities through its anti-inflammatory action in the treatment of migraine, arthritis, and atherosclerosis. To decide whether this effect applies to ischemic injury in brain, we therefore investigate the potential neuroprotective role of PN and the underlying mechanisms. Male Sprague-Dawley rats were randomly divided into Saline, Vehicle, and PN groups and a permanent middle cerebral artery occlusion (MCAO) model was used. PN administered intraperitoneally immediately after cerebral ischemia and once daily on the following days. At time points after MCAO, neurological deficit, infarct volume, and brain water content were measured. Immunohistochemistry, western blot and RT-PCR were used to analyze the expression of NF-κB and caspase-1 in ischemic brain tissue. Phospho-p38MAPK and claudin-5 were detected by western blot. The results indicated that PN dramatically ameliorated neurological deficit, brain water content, and infarct volume, downregulated NF-κB, phospho-p38MAPK, and caspase-1 expressions, and upregulated claudin-5 expression in ischemic brain tissue.Conclusions.PN protected the brain from damage caused by MCAO; this effect may be through downregulating NF-κB, phosho-p38MAPK, and caspase-1 expressions and ameliorating BBB permeability.

scholarly journals CEREBRAL VENOUS THROMBOSIS SECONDARY TO SARS-COV-2 INFECTION

2021 ◽  
Vol 5 (4) ◽  
Author(s):  
Heng Gee Lee ◽  
Heng Gee Lee ◽  
Heng Gee Lee ◽  
Heng Gee Lee
Keyword(s):  
Venous Thrombosis ◽  
Neurological Deficit ◽  
Cerebral Venous Thrombosis ◽  
Onset Seizure ◽  
Rare Form ◽  
Focal Neurological Deficit ◽  
Younger Women ◽  
First Onset

Cerebral venous thrombosis (CVT) is a relatively rare form of neurovascular emergency, and may present as headache, seizure, or focal neurological deficit. It typically has a higher occurrence in younger women. Recently, there are increasingly cases of CVTreported in association with COVID-19, which fall outside the typical demographics, suggesting a hyper-coagulable state attributable to COVID-19. Here, we present a case of CVTin a young gentleman with concomitant COVID-19, who presented with first-onset seizure.

CSF acid-base regulation and ventilation during acute hypercapnia in the newborn dog

1981 ◽  
Vol 50 (3) ◽  
pp. 566-574 ◽  
Author(s):  
E. E. Nattie ◽  
W. H. Edwards
Keyword(s):  
Water Content ◽  
Brain Tissue ◽  
Ionic Composition ◽  
Respiratory Acidosis ◽  
Tissue Water Content ◽  
Acid Base ◽  
Tissue Water ◽  
Plasma Ions ◽  
Ionic Distribution ◽  
Acid Base Status

We studied the response of blood, cerebrospinal fluid (CSF), and brain ionic composition and acid-base status as well as ventilation to acute respiratory acidosis (FICO2 0.08) in lightly anesthetized newborn puppies. Control puppy plasma ions and CSF-plasma ionic distribution ratios were essentially adultlike while in blood a mild, compensated respiratory acidosis was present, and in CSF, PCO2 and [HCO3-] were slightly higher than in adults. Brain tissue water content was higher in puppy vs. adult; the Cl- space was greater; the content of [Na+], [Cl-], and [HCO3-] were higher and [K+] lower. During respiratory acidosis, CSF [HCO3-] increased 2.0 mmol/l by 15 min and 6.2 mmol/l by 3 h, a response quantitatively like that observed in the adult. The quantity, CSF [Na+] -- [Cl-], increased stoichiometrically with CSF [HCO3-], indicating the mechanistic involvement of these ions in the CSF [HCO3-] response. In brain tissue, water content, [Cl-], and the [Cl-] space were unchanged, but by 3 h [Na+] and [HCO3-] were increased. Ventilation was stimulated but the response expressed as ml.min-1.Torr-1.body wt-1 was less in puppy than in adult.

Ischemic Brain Tissue Water Content: CT Monitoring during Middle Cerebral Artery Occlusion and Reperfusion in Rats1

Radiology ◽  
2007 ◽  
Vol 243 (3) ◽  
pp. 720-726 ◽  
Author(s):  
Imanuel Dzialowski ◽  
Ernst Klotz ◽  
Sophia Goericke ◽  
Arnd Doerfler ◽  
Michael Forsting ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Water Content ◽  
Middle Cerebral Artery Occlusion ◽  
Brain Tissue ◽  
Artery Occlusion ◽  
Tissue Water Content ◽  
Tissue Water ◽  
Ischemic Brain ◽  
Ischemic Brain Tissue ◽  
Cerebral Artery Occlusion
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