Effect of Theaflavin on Inflammatory and Remolding of Airway in the Asthma Mice

2021 ◽  
Vol 11 (6) ◽  
pp. 1091-1098
Author(s):  
Jingju Hu ◽  
Jing Yang ◽  
Hua Guo ◽  
Xuesong Yao ◽  
Haiyan Qiu ◽  
...  
Keyword(s):  
Interleukin 4 ◽  
Control Group ◽  
Model Group ◽  
Interleukin 5 ◽  
Treatment Groups ◽  
Interleukin 13 ◽  
Asthma Model ◽  
Lung Resistance ◽  
Neutrophile Granulocytes ◽  
Number Of Cells

To study the effect of theaflavin on the airway’s inflammation and remodeling in mice with asthma. The mice were divided into the control, asthma model, and the theaflavin treatment groups to analyze the changes in pulmonary compliance and lung resistance of the mice with asthma to theaflavin treatment. The theaflavin treatment groups consisted of the low-dose (15 mg/kg theaflavin-intragastric administration), medium-dose (30 mg/kg), and high-dose (60 mg/kg) groups. Alveoli lavage liquid was gathered from the mice to count the number of inflammatory cells, and the levels of interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 13 (IL-13), and eotaxin were detected by ELISA. The levels of proteins, such as transforming growth factor-1 (TGF-1), alpha-smooth muscle actin (α-SMA), CyclinD1,CyclinD2, Toll-like receptors-4 (TLR4), myeloid differentiation factor 88 (MyD88), and NF-κBp65, which showed the performance of lung tissue was tested by Western blotting. Compared to the control group, the lung resistance of the asthma model mice was increased, and compliance was decreased after increasing concentrations of acetylcholine (Mch) stimulation. Compared to the asthma model group, the pulmonary resistance was decreased, and pulmonar compliance was increased according to the rising concentration of Mch in theaflavin-L, theaflavin-M and theaflavin-H mice. Compared to the control group, the number of cells, macrophages, acidophilic cells, lymph, and neutrophile granulocytes increased in the alveolar perfusion fluid of asthmatic mice. The level of interleukin 4, interleukin 5, interleukin 13, and eotaxin, TGF-β1, α-SMA, Cyclin D1, MyD88, TLR4, Cyclin D2, and NF-κBp65 proteins of the lung was also increased. Compared to the model group, the number of cells, macrophages, acidophilic cells, lymph, and neutrophile granulocytes were decreased successively in the alveolar lavage fluid in the theaflavin-L, theaflavin-M, and theaflavin-H mice. Meanwhile, the content of interleukin 4, interleukin 5, interleukin 13, and eotaxin were decreased successively, and the level of TGF-β1, α-SMA, Cyclin D1, MyD88, TLR4, Cyclin D2, and NF-Bp65 protein increased successively in the theaflavin-L, theaflavin-M, and theaflavin-H mice. Theaflavin has been found to reduce airway inflammation, impede airway remodeling, and decrease the TLR 4/MyD88/NF-B signaling in asthmatic mice.

scholarly journals The effect of Nigella sativa and zinc on IgE and IL-5 serum levels, the experimental study in ovalbumin induced BALB/C mice

2021 ◽  
Vol 9 (4) ◽  
pp. 955
Author(s):  
Fany D. Rahmawati ◽  
Suprihati . ◽  
Yanuar I. Santosa ◽  
Willy Yusmawan ◽  
Anna M. K. Dewi ◽  
...  
Keyword(s):  
Mast Cells ◽  
Nigella Sativa ◽  
Serum Levels ◽  
Specific Ige ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Interleukin 5 ◽  
Treatment Groups ◽  
Post Test

Background: Allergy is a hypersensitivity reaction due to induction of specific IgE binding to allergens on the surface of mast cells. Interleukin-5 is an important marker of hypersensitivity inflammation reactions.  Nigella sativa, contain active substance thymoquinone, can reduce inflammatory mediators. Zinc as anti-inflammatory by inhibit releasing mediators from mast cells. Objective was to determine the effect of Nigella sativa (NS) and zinc on IgE and IL-5 serum levels on ovalbumin-induced BALB/C mice.Methods: The study design was a true experiment with post-test only control group using BALB/C mice. The study was conducted at Sultan Agung Islamic University’s laboratory on March-June 2020. Inclusion criteria were female BALB C mice, 6-8 weeks, 22-25 grams, and healthy. Thirty mice were divided randomly into 5 groups; negative control, positive control, NS group, zinc group, and NS + zinc group. All groups treated for 28 days. Allergic reactions tested by skin test with OVA, intervention response assessed by IgE and IL-5 serum levels.Results: At the end of study completed, obtained 6 negative controls, 6 positive controls, 6 NS groups, 5 zinc groups, and 5 NS+zinc groups. Two mice s died before intervention completed. Serum IgE and IL5 levels were significantly difference between treatment groups (Kruskal Wallis test; p=0.007 and One-way ANOVA test; p=0.020). The result of logistic regression test, IgE levels was the most significant in the NS+zinc group (p=0.006) and IL-5 levels was the most significant in the zinc group (p=0.002)Conclusions:  Nigella sativa, zinc, and its combination can reduce IgE and IL-5 serum levels of ovalbumin-induced BALB / C mice.

Tyrosol improves ovalbumin (OVA)-induced asthma in rat model through prevention of airway inflammation

2021 ◽  
Author(s):  
Mustafa Cellat ◽  
Müslüm Kuzu ◽  
Cafer Tayer İşler ◽  
Muhammed Etyemez ◽  
Nursel Dikmen ◽  
...  
Keyword(s):  
Control Group ◽  
Tissue Architecture ◽  
Oral Gavage ◽  
Mucus Hypersecretion ◽  
Treatment Groups ◽  
Asthma Model ◽  
Tnf Α ◽  
Pas Staining ◽  
Gpx Activity ◽  
Histopathological Investigation

Abstract Purpose Asthma is an inflammatory disease that affects many people around the world, especially individuals of pediatric age. The effectiveness of tyrosol, a natural phenolic compound, was examined in the asthma model induced by ovalbumin (OVA). Methods For this purpose, 4 groups, each consisting of 8 rats, were formed. Serum physiological was given to the control group for 21 days. OVA was given to OVA, OVA + Dexamethasone (Dexa) and OVA + tyrosol groups intraperitoneally and by inhalation. Additionally, 0.25 mg/kg Dexa was administered to the OVA + Dexa group and 20 mg/kg tyrosol to the OVA + Tyrosol group by oral gavage. Serum, blood, BALF fluid and lung tissues of the rats were examined. Results it was observed that the MDA level decreased, GSH level and GPx activity increased, and there was no change in CAT activity in the tyrosol treatment groups. It was also observed that NF-κB, TNF-α, IL-4, IL-5, IL-13, IFN-𝛾, and IgE levels decreased compared to the OVA group. However, no effect on IL-1 β level was observed. In addition, it was determined that tyrosol treatment increased the IL-10 level. The results of the histopathological investigation of lung tissue showed that tyrosol significantly ameliorated OVA-induced histopathological lesions. Additionally, PAS staining showed that mucus hypersecretion was significantly reduced with the use of tyrosol. In addition, it was determined that the number of eosinophils decreased significantly. Conclusions The obtained results showed that tyrosol presented antioxidant and anti-inflammatory features on OVA-induced rats and preserved tissue architecture.

Interleukin 4 and the Related Cytokines (Interleukin 5 and Interleukin 13)

2016 ◽  
pp. 678-686
Author(s):  
Chrysanthi Skevaki ◽  
Christoph Hudemann ◽  
Harald Renz
Keyword(s):  
Interleukin 4 ◽  
Interleukin 5 ◽  
Interleukin 13

scholarly journals Effects of Deep Electroacupuncture Stimulation at “Huantiao” (GB 30) on Expression of Apoptosis-Related Factors in Rats with Acute Sciatic Nerve Injury

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Lili Dai ◽  
Yanjing Han ◽  
Tieming Ma ◽  
Yuli Liu ◽  
Lu Ren ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Normal Control ◽  
Expression Level ◽  
Control Group ◽  
Model Group ◽  
Related Factors ◽  
Treatment Groups ◽  
Bax Protein ◽  
Hip Muscle ◽  
Nerve Muscle

SD rats were randomly divided into normal control, model, deep EA, and shallow EA groups. The model was established by mechanical clamping of the sciatic nerve stem. For deep and shallow EA, the needles were inserted into “Huantiao” (GB 30) by about 16 mm and 7 mm, respectively, once daily for 14 days. The results showed that, compared with the normal control group, the nerve-muscle excitability of rat’s hip muscle decreased and the nerve conduction velocity of sciatic nerve slowed down in the model group; meanwhile, the number of apoptotic cells and the expression level of Bax protein in the injured nerve increased significantly, and the expression level of Bcl-2 protein and the ratio of Bcl-2/Bax decreased considerably. Compared with the model group, the indices mentioned above were reversed in the two treatment groups, and the changes in the deep EA group were more significant than those in the shallow EA group. These results indicate that EA stimulation at GB 30 can improve the function of injured sciatic nerve, which is closely associated with its effects in upregulating the expression of apoptosis inhibitive factor Bcl-2 and downregulating apoptosis promotive factor Bax. Deep EA is relatively better.

Effect of Curcumin on Diabetes in an Alloxan Mice Model

2014 ◽  
Vol 1051 ◽  
pp. 363-367 ◽  
Author(s):  
Ming San Miao ◽  
Shuo Tian ◽  
Lin Guo
Keyword(s):  
Positive Control ◽  
Normal Control Group ◽  
Control Group ◽  
Group Level ◽  
Model Group ◽  
Mice Model ◽  
Curcumin Treatment ◽  
Blank Group ◽  
Treatment Groups ◽  
Different Doses

Objective: Research on the effect of Curcumin on diabetes in an alloxan mice model. Methods: The positive control group was given Diamicron suspension; High, medium and low treatment groups were given different doses of curcumin solution; Blank group and model group are given the same volume of saline. When the tenth, twentieth, thirtieth day of the administration, measured the blood glucose (BG) of the mice, after the last administration, observed pathological changes of the pancreas under light microscope. Results: Compared with normal control group, the model group level of BG were significantly higher in 10 days,20days,30days and the emergence of pancreatic lesions. Curcumin treatment groups were significantly reduced these abnormalities (P<0.05,P<0.01).Conclusion:Curcumin can reduce the lever of BG at the early diabetic mice and improved the pancreatic lesions caused by diabetes.

scholarly journals Ganoderic Acid A Alleviates OVA-Induced Asthma in Mice

Inflammation ◽  
2021 ◽  
Author(s):  
Xinhua Lu ◽  
Chenyang Xu ◽  
Rui Yang ◽  
Guojun Zhang
Keyword(s):  
Lung Tissue ◽  
Lung Inflammation ◽  
Inflammatory Cell ◽  
Inflammatory Cells ◽  
Interleukin 4 ◽  
Model Group ◽  
Ganoderic Acid ◽  
Tissue Sections ◽  
Asthma Model

AbstractThe aim of this study is to investigate the effects of ganoderic acid A (GAA) on OVA-induced asthma in mice. Mouse asthma model was established by ovalbumin (OVA) in vitro. Diff-Quik staining was used to observe the total numbers of cells and the number of classification cells in each group, and HE staining was used to observe lung inflammation in lung tissue sections. ELISA was used to detect the effect of GAA on the levels of interleukin-4 (IL-4), IL-5, and IL-13 in serum and lung tissue. The expression levels of TLR/NF-κB were detected by Western blot. Immunohistochemistry was used to observe the expression changes of TLR4 and P-P65. Compared with the normal group, the inflammatory cell count, IL-4, IL-5, and IL-13 expression in the model group increased, and TLR/NF-kB signal protein expression increased. Compared with the model group, in GAA group, the number of inflammatory cells, the expression of IL-4, IL-5, and IL-13 decreased, and the expression of TLR/NF-kB signaling protein decreased. GAA regulated lung inflammation in asthmatic mice by inhibiting TLR/NF-kB signaling pathway.

scholarly journals Effects of Concentrated Growth Factor and Nanofat on Aging Skin of Nude Mice Induced by D-Galactose

2021 ◽  
pp. 425-435
Author(s):  
W SUN ◽  
T LI ◽  
H YAO ◽  
L KANG ◽  
F DONG
Keyword(s):  
Growth Factor ◽  
Nude Mice ◽  
Type I Collagen ◽  
Elastic Fiber ◽  
Control Group ◽  
Type Iii Collagen ◽  
Type I ◽  
Model Group ◽  
Treatment Groups ◽  
Aging Skin

This investigation studied the effect of concentrated growth factor and nanofat on aging skin of nude mice induced by D-galactose. BALB/c mice were randomly divided into five groups: 5 mice in the control group were fed normally without any intervention, 9 mice were treated with concentrated growth factor (CGF), 9 mice were treated with nanofat (NF), 9 mice were treated with CGF+NF, and 9 mice in the model group (no treatment after subcutaneous injection of D-galactose). Relevant indicators are measured and recorded. In skin and serum, SOD and GSH content in the model group were significantly lower than those in other groups (P<0.05), and the MDA of the three treatment groups was significantly lower than that of the model group (P<0.05). Compared with the control group, the contents of total collagen, type I collagen and type III collagen in the NF group and model group were decreased in different degrees (P<0.05); the contents of elastin and elastic fiber in the skin of nude mice in the model group and NF group were significantly decreased. Compared with the model group, he number of CD31 and VEGF in the treatment group was significantly increased (P<0.01); the skin AGE content of three treatment groups was significantly lower (P<0.05). These findings suggest that concentrated growth factor and nanofat may have a significant effect on delaying aging skin induced by D-galactose in nude mice.

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