Nanofibrous Glass Tailored with Apatite-Fibronectin Interface for Bone Cell Stimulation

2008 ◽  
Vol 8 (6) ◽  
pp. 3013-3019 ◽  
Author(s):  
Hae-Won Kim ◽  
Hae-Hyoung Lee ◽  
Jonathan C. Knowles
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Bone Mineral ◽  
Bone Matrix ◽  
Bone Cell ◽  
Cell Stimulation ◽  
Adhesive Protein ◽  
Cell Adhesive ◽  
A Cell

Exploring a material with smart and biomimetic interface has great potential in the biomaterials and tissue engineering field. This paper reports a novel nanofibrous bone matrix that was developed to retain a cell-stimulating and bone-mimetic biointerface. The bone mineral, apatite, and the cell adhesive protein, fibronectin (FN), were hybridized on the interface of a bioactive glass nano-fibrous mesh, through the dissolution-and-reprecipitation process. The hybridized nanofibrous mesh showed significant improvement in the initial responses of the bone-derived cells. It is believed that this biomimetic and cell-stimulating nanofibrous mesh can be used as a potential bone regeneration matrix.

scholarly journals Comparison of the Effect of Sol-Gel and Coprecipitation Routes on the Properties and Behavior of Nanocomposite Chitosan-Bioactive Glass Membranes for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Elke M. F. Lemos ◽  
Sandhra M. Carvalho ◽  
Patrícia S. O. Patrício ◽  
Claudio L. Donnici ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Sol Gel ◽  
Composite Films ◽  
Maximum Tensile Stress ◽  
Control Group ◽  
Nanoparticle Dispersion

Recent studies in tissue engineering have highlighted the importance of the development of composite materials based on biodegradable polymers containing bioactive glasses, in particular, composites for high load support and excellent cell viability for potential application in bone regeneration. In this work, hybrid composite films were obtained by combining chitosan with bioactive glass in solution form and in nanoparticle dispersion form obtained by the two different synthesis routes: the sol-gel method and coprecipitation. The bioactive glass served both as a mechanical reinforcing agent and as a triggering agent with high bioactivity. The results ofin vitroassays with simulated body fluid demonstrated the formation of a significant layer of fibrils on the surface of the film, with a typical morphology of carbonated hydroxyapatite, reflecting induction of a favorable bioactivity. Maximum tensile stress increased from 42 to 80 MPa to the sample with 5% wt bioactive glass. In addition, samples containing 5% and 10% wt bioactive glass showed a significant increase in cell viability, 18 and 30% increase compared to the control group. The samples showed significant response, indicating that they could be a potential material for use in bone regeneration through tissue engineering.

scholarly journals Local delivery of a novel PTHrPviamesoporous bioactive glass scaffolds to improve bone regeneration in a rat posterolateral spinal fusion model

RSC Advances ◽  
2018 ◽  
Vol 8 (22) ◽  
pp. 12484-12493 ◽  
Author(s):  
Bo Liang ◽  
Jinghuan Huang ◽  
Jianguang Xu ◽  
Xiaolin Li ◽  
Jingfeng Li
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Spinal Fusion ◽  
Bone Substitutes ◽  
Bone Defects ◽  
Local Delivery ◽  
Long Bones ◽  
Fusion Model ◽  
Posterolateral Spinal Fusion

With the development of tissue engineering, bone defects, such as fractured long bones or cavitary lesions, may be efficiently repaired and reconstructed using bone substitutes.

scholarly journals Characterisation of bone regeneration in 3D printed ductile PCL/PEG/hydroxyapatite scaffolds with high ceramic microparticle concentrations

2021 ◽  
Author(s):  
Jing Yang ◽  
Chuanliang Cao ◽  
Pengren Huang ◽  
Aruna Prasopthum ◽  
Andy James Parsons ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Particle Concentration ◽  
Composite Scaffolds ◽  
Reinforced Composite ◽  
3D Printed ◽  
Particle Reinforced Composite ◽  
Very High

3D printed bioactive glass or bioceramic particle reinforced composite scaffolds for bone tissue engineering currently suffer from low particle concentration (<50 wt%) hence low osteoconductivity. Meanwhile, composites with very high...

Peroxinectin, a cell adhesive protein associated with the proPO system from the black tiger shrimp, Penaeus monodon

2001 ◽  
Vol 25 (5-6) ◽  
pp. 353-363 ◽  
Author(s):  
K. Sritunyalucksana ◽  
K. Wongsuebsantati ◽  
M.W. Johansson ◽  
K. Söderhäll
Keyword(s):  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Adhesive Protein ◽  
Tiger Shrimp ◽  
Cell Adhesive ◽  
A Cell

Preparation and Characterization of Biomimetic Mesoporous Bioactive Glass-Silk Fibroin Composite Scaffold for Bone Tissue Engineering

2013 ◽  
Vol 796 ◽  
pp. 9-14 ◽  
Author(s):  
Cai Hong Lei ◽  
Xin Xing Feng ◽  
Ya Yang Xu ◽  
Yue Rong Li ◽  
Hai Lin Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Composite Scaffold ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Composite Scaffolds ◽  
Mesoporous Bioactive Glass

Three-dimensional (3D) mesoporous bioactive glass (MBG) scaffolds were obtained by using the demineralized bone matrix (DBM) and P123 as co-templates through a dip-coating method followed by evaporation induced self-assembly (EISA) process. 3D mesoporous bioactive glass-silk fibroin (MBG/SF) composite scaffolds were prepared by immersing MBG scaffolds into SF solutions with different concentration. Transmission electron microscopy (TEM), field mission scanning electron microscope (FESEM), fourier transform infrared spectroscopy (FT-IR) and wide angle X-ray diffraction (WA-XRD) were used to analyze the inner pore structures, pore sizes, morphologies and composition of the scaffolds. The in vitro bioactivity of the scaffolds was evaluated by soaking in simulated body fluid (SBF). The results showed that the MBG and MBG/SF composite scaffolds with the interconnected macroporous network and mesoporous walls could be obtained by this method. In addition, both the MBG scaffolds and the MBG/SF composite scaffolds have excellent apatite-forming bioactivity. Therefore, this method provides a simple way to prepare scaffolds for bone tissue engineering.

scholarly journals Porosity Pattern of 3D Chitosan/Bioactive Glass Tissue Engineering Scaffolds Prepared for Bone Regeneration

2021 ◽  
Vol 15 (1) ◽  
pp. 41-56
Author(s):  
Hoda G.H. Hammad ◽  
Miral Nagy F. Salama
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Phase Identification ◽  
Tissue Engineering Scaffolds ◽  
Composite Scaffolds ◽  
X Ray ◽  
Pore Sizes ◽  
Porous Chitosan ◽  
Scanning Electron

Aim: The study was conducted to investigate the obtained external and internal porosity and the pore-interconnectivity of specific fabricated bioactive composite tissue engineering scaffolds for bone regeneration in dental applications. Materials and Methods: In this study, the bioactive glass [M] was elaborated as a quaternary system to be incorporated into the chitosan [C] scaffold preparation on a magnetic stirrer to provide bioactivity and better strength properties for the attempted composite scaffolds [C/ M] of variable compositions. The homogenous chitosan/bioactive glass mix was poured into tailor-made cylindrical molds [10cm×10cm]; a freeze-dryer program was used for the creation of uniform and interconnected macropores for all prepared chitosan-based scaffolds. The morphology of fabricated chitosan [C] and chitosan-bioactive glass [C/ M] composite scaffolds was studied by a scanning electron microscope [SEM] and a mercury porosimeter. In addition, the in-vitro biodegradation rate of all elaborated scaffolds was reported after immersing the prepared scaffolds in a simulated body fluid [SBF] solution. Furthermore, for every prepared scaffold composition, characterization was performed for phase identification, microstructure, porosity, bioactivity, and mechanical properties using an X-ray diffraction analysis [XRD], an X-ray Fourier transfer infrared spectroscopy [FTIR], a mercury porosimetry, a scanning electron microscopy [SEM] coupled to an energy-dispersive X-ray spectrometry [EDS] and a universal testing machine, respectively. Results: All the prepared porous chitosan-based composite materials showed pore sizes suitable for osteoblasts seeding, with relatively larger pore sizes for the C scaffolds. Conclusion: The smart blending of the prepared bioactive glass [M] with the chitosan matrix offered some advantages, such as the formation of an apatite layer for cell adhesion upon the scaffold surfaces, the reasonable decrease in scaffold pore size, and the relative increase in compressive strength that were enhanced by the incorporation of [M]. Therefore, the morphology, microstructure, and mechanical behavior of the elaborated stress loaded biocomposite tissue engineering scaffolds seem highly dependent on their critical contented bioactive glass.

Surface Roughness Values Closer to Bone for Titania Nanoparticle/Poly-lactic-co-glycolic Acid (PLGA) Composites Increases Bone Cell Adhesion

2005 ◽  
Vol 873 ◽  
Author(s):  
Huinan Liu ◽  
Elliott B. Slamovich ◽  
Thomas J. Webster
Keyword(s):  
Tissue Engineering ◽  
Surface Roughness ◽  
Cell Adhesion ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Glycolic Acid ◽  
Ceramic Materials ◽  
Bone Substitutes ◽  
Bone Cell ◽  
Osteoblast Adhesion

AbstractBone substitutes are often required to replace damaged tissue due to injuries, diseases and genetic malformations. Traditional bone substitutes, such as autografts, allografts, xenografts and metal implants, are far from ideal as each have their own specific problems and limitations. Bone tissue engineering offers a promising opportunity for bone regeneration in a natural way. However, currently the scientific challenges of bone tissue engineering lie in the development of suitable scaffold materials that can improve bone cell adhesion, proliferation and differentiation. The design of nanophase titania/polymer composites offers an exciting approach to combine the advantages of a degradable polymer with nano-size ceramic grains that optimize biological properties for bone regeneration. Importantly, nanophase titania mimics the size scale of constituent components of bone since bone itself is a nanostructured composite composed of nanometer hydroxyapatite crystals well-dispersed in a mostly collagen matrix. Previous studies have shown significant improvement in protein adsorption, osteoblast (bone-forming cell) adhesion and long-term functions on nano-grain ceramic materials compared to traditional micron-grain ceramic materials. This study used nanometer grain size titania dispersed in a model polymer (PLGA or poly-lactic-co-glycolic acid) matrix by using various sonication powers to increase osteoblast adhesion. The surface characteristics of the composites, such as topography, titania surface area coverage and surface roughness, were studied by scanning electron microscopy and atomic force microscopy. Of all the composites formulated in this study, osteoblast adhesion was the greatest on nanophase titania/PLGA (30/70 wt.%) sonicated at 118.75 for 10 minutes; this composite was the closest in terms of nanometer surface roughness compared to bone of all the composites formulated. In this manner, this study suggests that nanophase titania sonicated in PLGA under these conditions should be further studied for orthopedic applications.

Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 49144-49152 ◽  
Author(s):  
Sepehr Talebian ◽  
Mehdi Mehrali ◽  
Saktiswaren Mohan ◽  
Hanumantha rao Balaji raghavendran ◽  
Mohammad Mehrali ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Differentiation ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Cell ◽  
Hybrid Nanofibers ◽  
Bone Cell Differentiation

Incorporation of bioactive glass into chitosan (PEO) nanofibers leads to improvement of strength and bone-cell differentiation capability.

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