Di-Sulfide Linked Polyethylenimine Coated Gold Nanoparticles as a Non-Viral Gene Delivery Agent in NIH-3T3 Mouse Embryonic Fibroblast

2015 ◽  
Vol 15 (10) ◽  
pp. 7895-7899 ◽  
Author(s):  
Saji Uthaman ◽  
Myeong Ju Moon ◽  
Duhwan Lee ◽  
Won Jong Kim ◽  
In-Kyu Park
Keyword(s):  
Gold Nanoparticles ◽  
Gene Delivery ◽  
Fluorescent Microscopy ◽  
Weight Ratio ◽  
Mouse Embryonic Fibroblast ◽  
Luciferase Assay ◽  
Viral Gene ◽  
Hydrodynamic Diameter ◽  
Tem Analysis ◽  
Average Size

Di-sulfide linked polyethylenimine coated gold nanoparticles (ssPEI-GNPs) of 20 nm size was prepared in order to deliver the genes to target site. DLS and TEM analysis demonstrated that the GNPs have average size of 13 nm in diameter. Upon coating the GNPs with ssPEI in the weight ratio of 1:3, the average hydrodynamic diameter of the ssPEI-GNPs was found to 19 ± 1.14 nm and a zeta potential value 41 ± 1.23 mV was observed. TEM analysis of ssPEI-GNPs demonstrated that the nanoparticles have spherical morphology. Thermogravemetric analysis of the prepared ssPEI-GNPs showed that the estimated composition of the ssPEI coated over the GNPs was approximately 5% (w/w). Gene expression capabilities of the nanoparticles were confirmed by fluorescent microscopy and luciferase assay, which demonstrated the transgene delivery capability of the ssPEI-GNPs. These results demonstrate that ssPEI-GNPs could be used as gene delivery agent.

scholarly journals Biosynthesis of Gold Nanoparticles (Green-gold) Using Leaf Extract ofTerminalia Catappa

2010 ◽  
Vol 7 (4) ◽  
pp. 1334-1339 ◽  
Author(s):  
Balaprasad Ankamwar
Keyword(s):  
Gold Nanoparticles ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Low Cost ◽  
Attractive Potential ◽  
Chloroauric Acid ◽  
Rapid Reduction ◽  
Tem Analysis ◽  
Terminalia Catappa ◽  
Average Size

The synthesis of eco-friendly nanoparticles is evergreen branch of nanoscience for biomedical application. Low cost of synthesis and non toxicity are main features make it more attractive potential option for biomedical field and elsewhere. Here, we report the synthesis of gold nanoparticles in aqueous medium usingTerminalia catappa(Almond) leaf extract as the reducing and stabilizing agent. On treating chloroauric acid solutions withTerminalia catappa(TC) leaf extract rapid reduction of chloroaurate ions is observed leading to the formation of highly stable gold nanoparticles in solution. TEM analysis of the gold nanoparticles indicated that they ranged in size from 10 to 35 nm with average size of 21.9 nm.

scholarly journals Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Joydeep Das ◽  
Yun-Jung Choi ◽  
Hideyo Yasuda ◽  
Jae Woong Han ◽  
Chankyu Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Gene Transfection ◽  
Adipogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Luciferase Assay ◽  
Beta Gene ◽  
Lipofectamine 2000

Abstract The controlled differentiation of stem cells via the delivery of specific genes encoding appropriate differentiation factors may provide useful models for regenerative medicine and aid in developing therapies for human patients. However, the majority of non-viral vectors are not efficient enough to manipulate difficult-to-transfect adult human stem cells in vitro. Herein, we report the first use of 25 kDa branched polyethylenimine-entrapped gold nanoparticles (AuPEINPs) and covalently bound polyethylenimine-gold nanoparticles (AuMUAPEINPs) as carriers for efficient gene delivery into human mesenchymal stem cells (hMSCs). We determined a functional application of these nanoparticles by transfecting hMSCs with the C/EBP beta gene, fused to EGFP, to induce adipogenic differentiation. Transfection efficacy with AuPEINPs and AuMUAPEINPs was 52.3% and 40.7%, respectively, which was 2.48 and 1.93 times higher than that by using Lipofectamine 2000. Luciferase assay results also demonstrated improved gene transfection efficiency of AuPEINPs/AuMUAPEINPs over Lipofectamine 2000 and polyethylenimine. Overexpression of exogenous C/EBP beta significantly enhanced adipogenesis in hMSCs as indicated by both of Oil Red O staining and mRNA expression analyses. Nanoparticle/DNA complexes exhibited favorable cytocompatibility in hMSCs. Taken together, AuPEINPs and AuMUAPEINPs potentially represent safe and highly efficient vehicles for gene delivery to control hMSC differentiation and for therapeutic gene delivery applications.

scholarly journals Biosynthesis of gold nanoparticles using psidium guajava leaf extract

2014 ◽  
Vol 9 (3) ◽  
Author(s):  
Amel Taha ◽  
Mustaffa Shamsuddin
Keyword(s):  
Gold Nanoparticles ◽  
Metal Ion ◽  
Leaf Extract ◽  
Psidium Guajava ◽  
Ion Concentration ◽  
Tem Analysis ◽  
Synthesis Of Nanoparticles ◽  
Size Of Particles ◽  
Average Size ◽  
The Cost

Nanomaterials are attracting a lot of attention nowadays as they show different chemical and physical properties that are dependent on their size and shape. Different techniques have been used for synthesis metal nanoparticles but the use of plants for synthesis of nanoparticles could be advantageous over other environmentally methods as this eliminates the cost and the non toxicity is main feature. Here a simple biosynthesis approach was applied and gold nanoparticles were synthesized by using psidium guajava leaf extract as reducing and capping agent. Two different parameters were studied which are the metal ion concentration and volume of plant broth. The synthesis of gold nanoparticles was also monitored as function of reaction time. The formation of gold nanoparticles was monitored by UV-Vis spectrophotometer and the size of particles was confirmed by X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM) techniques. TEM analysis showed that the AuNPs were mostly spherical in shape with an average size of 15 nm. Fourier Transform Infrared (FTIR) spectroscopy that the active biomolecules present in the Psidium guajava leaf extract act as capping and stabilizing agent for the AuNPs.

scholarly journals Combinatorial library of biodegradable polyesters enables delivery of plasmid DNA to polarized human RPE monolayers for retinal gene therapy

2018 ◽  
Author(s):  
Bibhudatta Mishra ◽  
David R. Wilson ◽  
Srinivas R. Sripathi ◽  
Mark P. Suprenant ◽  
Yuan Rui ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
High Throughput Screening ◽  
High Efficiency ◽  
Polymeric Nanoparticles ◽  
Retinal Pigment ◽  
Weight Ratio ◽  
Retinal Cell ◽  
Viral Gene ◽  
Rpe Cells

Abstract:Efficient gene delivery into hard-to-transfect cells is still a challenge despite significant progress in the development of various gene delivery tools. Non-viral and synthetic polymeric nanoparticles offer an array of advantages for gene delivery over the viral vectors and high in demand as they are safe to use, easy to synthesize and highly cell-type specific. Here we demonstrate the use of a high-throughput screening (HTS) platform to screen for biodegradable polymeric nanoparticles (NPs) that can transfect human retinal pigment epithelial (RPE) cells with high efficiency and low toxicity. These NPs can deliver plasmid DNA (pDNA) to RPE monolayers more efficiently compared to the commercially available transfection reagents without interfering the global gene expression profile of RPE cells. In this work, we have established an HTS platform and identified synthetic polymers that can be used for high efficacy non-viral gene delivery to human RPE monolayers, enabling gene loss- and gain-of-function studies of cell signaling and developmental pathways. This platform can be used to identify the optimum polymer, weight-to-weight ratio of polymer to DNA, and the dose of NP for various retinal cell types.

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

Preparation of Cationic Gold Nanoparticles for Gene Delivery

2008 ◽  
Vol 2 (2) ◽  
pp. 152-156 ◽  
Author(s):  
Guoping Chen ◽  
Michiaki Takezawa ◽  
Naoki Kawazoe ◽  
Tetsuya Tateishi
Keyword(s):  
Gold Nanoparticles ◽  
Gene Delivery ◽  
Cationic Gold

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

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