Thermal and Spectroscopic Analyses of Human Adipose Tissue-Derived Extracellular Matrix

2021 ◽  
Vol 21 (7) ◽  
pp. 3662-3666
Author(s):  
Hyun-Ji Lim ◽  
Tae-Yub Kwon
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Microscopic Analysis ◽  
Human Adipose Tissue ◽  
Absorption Feature ◽  
Raman Signal ◽  
Rapid Weight Loss ◽  
Electron Microscopic ◽  
Spectroscopic Analyses ◽  
Freeze Dried

In this study, nanofibrous extracellular matrix (ECM) was prepared from human adipose tissue, and the stepwise products were analyzed using differential thermal analysis (DTA)/thermogravimetric analysis (TGA) and Fourier-transform infrared (FTIR)/Raman spectroscopy. Human adipose tissue was liposuctioned (sample 1), centrifuged (sample 2), pulverized, centrifuged again (sample 3), and finally freeze-dried (sample 4). Each sample was subjected to DTA/TGA and FTIR/Raman analyses. In the DTA curve of sample 1, the major peak was observed at approximately 60 °C. However, relatively flat DTA curves were detected in samples 2 and 4. In the TGA results, sample 1 showed a more rapid weight loss pattern than the other samples. Samples 1, 2, and 3 showed similar FTIR spectra with a strong, broad absorption feature at ~3400 cm−1. Amide I, II, and III bands were clearly observed in the FTIR spectrum of sample 4. Samples 1 and 2 showed typical adipose tissue Raman spectra. However, in samples 3 and 4, the Raman signal was low. Scanning electron microscopic analysis of the freeze-dried ECM (sample 4) confimed its three-dimensional porous nanofibrous structure. These findings suggest that human adipose tissue, intermediate products, and human adipose tissue-derived ECM can be easily and effectively characterized with thermal and spectroscopic analyses. These efficient analyses can aid in the preparation of ECM and in clinical applications for regenerative medicine.

Recellularization of decellularized human adipose-tissue-derived extracellular matrix sheets with other human cell types

2012 ◽  
Vol 348 (3) ◽  
pp. 559-567 ◽  
Author(s):  
Beob Soo Kim ◽  
Ji Suk Choi ◽  
Jae Dong Kim ◽  
Young Chan Choi ◽  
Yong Woo Cho
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Human Cell ◽  
Human Adipose Tissue ◽  
Cell Types

Cardiac extracellular matrix hydrogel together with or without inducer cocktail improves human adipose tissue-derived stem cells differentiation into cardiomyocyte–like cells

2018 ◽  
Vol 502 (2) ◽  
pp. 215-225 ◽  
Author(s):  
Masoumeh Baghalishahi ◽  
Seyed hasan Efthekhar-vaghefi ◽  
Abbas Piryaei ◽  
S.N. Nematolahi-mahani ◽  
Hamid Reza Mollaei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Human Adipose Tissue

scholarly journals Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

2021 ◽  
Author(s):  
Svenja Nellinger ◽  
Ivana Mrsic ◽  
Silke Keller ◽  
Simon Heine ◽  
Alexander Southan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Cultured Cells ◽  
Structural Characteristics ◽  
Human Adipose Tissue ◽  
Minimal Invasive ◽  
Healthcare Applications ◽  
Decellularized Extracellular Matrix

Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively towards their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells (ASCs), towards their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans (sGAGs) compared to cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.

scholarly journals A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation

Processes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 88
Author(s):  
Ilaria Roato ◽  
Federico Mussano ◽  
Simone Reano ◽  
Filippo Boriani ◽  
Andrea Margara ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Stromal Vascular Fraction ◽  
Fold Increase ◽  
Human Adipose Tissue ◽  
Promising Tool ◽  
Filter Size ◽  
Tissue Recovery

This work aims to characterize a new method to recover low-manipulated human adipose tissue, enriched with adipose tissue-derived mesenchymal stem cells (ATD-MSCs) for autologous use in regenerative medicine applications. Lipoaspirated fat collected from patients was processed through Lipocell, a Class II-a medical device for dialysis of adipose tissue, by varying filter sizes and washing solutions. ATD-MSC yield was measured with flow cytometry after stromal vascular fraction (SVF) isolation in fresh and cultured samples. Purification from oil and blood was measured after centrifugation with spectrophotometer analysis. Extracellular matrix preservation was assessed through hematoxylin and eosin (H&E) staining and biochemical assay for total collagen, type-2 collagen, and glycosaminoglycans (GAGs) quantification. Flow cytometry showed a two-fold increase of ATD-MSC yield in treated samples in comparison with untreated lipoaspirate; no differences where reported when varying filter size. The association of dialysis and washing thoroughly removed blood and oil from samples. Tissue architecture and extracellular matrix integrity were unaltered after Lipocell processing. Dialysis procedure associated with Ringer’s lactate preserves the proliferation ability of ATD-MSCs in cell culture. The characterization of the product showed that Lipocell is an efficient method for purifying the tissue from undesired byproducts and preserving ATD-MSC vitality and extracellular matrix (ECM) integrity, resulting in a promising tool for regenerative medicine applications.

scholarly journals Human Adipose Tissue Derived Extracellular Matrix and Methylcellulose Hydrogels Augments and Regenerates the Paralyzed Vocal Fold

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0165265 ◽  
Author(s):  
Dong Wook Kim ◽  
Eun Ji Kim ◽  
Eun Na Kim ◽  
Myung Whun Sung ◽  
Tack-Kyun Kwon ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Vocal Fold ◽  
Human Adipose Tissue

Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue–Derived Stromal Cells

2001 ◽  
Vol 7 (6) ◽  
pp. 729-741 ◽  
Author(s):  
Yuan-Di C. Halvorsen ◽  
Dawn Franklin ◽  
Arden L. Bond ◽  
Daron C. Hitt ◽  
Catherine Auchter ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Matrix Mineralization

scholarly journals A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation

2019 ◽  
Author(s):  
Ilaria Roato ◽  
Federico Mussano ◽  
Simone Reano ◽  
Filippo Boriani ◽  
Andrea Margara ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Fold Increase ◽  
Human Adipose Tissue ◽  
Promising Tool ◽  
Filter Size ◽  
Novel Method ◽  
Tissue Recovery

This work aims to characterize a new method to recover low-manipulated human adipose tissue, enriched of adipose tissue-derived mesenchymal stem cells (ATD-MSCs) for autologous use in regenerative medicine applications. Lipoaspirated fat collected from patients was processed through Lipocell, a II-a medical device for dialysis of adipose tissue, by varying filter sizes and washing solutions. ATD-MSCs yield was measured with flow cytometry after SVF isolation in fresh and cultured samples. Purification from oil and blood was measured after centrifugation with spectrophotometer analysis. Extracellular matrix preservation was assessed through H&E staining and biochemical assay for total collagen, type-2 collagen, and GAGs quantification. Flow cytometry showed a 2-fold increase of ATD-MSCs yield in treated samples in comparison with untreated lipoaspirate; no differences where reported when varying filter size. The association of dialysis and washing thoroughly removed blood and oil from samples. Tissue architecture and extracellular matrix integrity were unaltered after Lipocell processing. Dialysis procedure associated with Ringer’s lactate preserves the proliferation ability of ATD-MSCs in cell culture. The characterization of the product shows that Lipocell is an efficient method to purify the tissue from undesired byproducts, preserving ATD-MSCs vitality and ECM integrity, resulting in a promising tool for regenerative medicine applications.

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