Effect of Ropivacaine nanoparticles on apoptosis of cerebral vascular endothelial cells

The objective of this study was to investigate the effect of Ropivacaine lactate glycolic acid copolymer nanoparticles on the apoptosis of vascular endothelial cells after cerebral vasospasm (CVS) in rabbits. Thirty New Zealand rabbits were randomly divided into five groups: Sham Operation, control, blank nanoparticles, nanoparticles drug-loaded, and Ropivacaine. A model of subarachnoid hemorrhage (SAH) was established by injecting blood into the rabbits' cisterna magna twice. The mean velocity (VM) and peak systolic velocity (VP) of basilar artery were compared at one, three, and seven days, after which the shape was observed under a light microscope. The diameter of the lumen was measured at seven days after injection. The TUNEL method was used to detect the apoptosis of endothelial cells in the basilar artery. There was no obvious edema or blood clot and the basilar artery was clear in the Sham Operation group. In the control and blank nanoparticles groups, there was obvious swelling of brain tissue, and obvious blood clots were found at the bottom of brain, mostly around the basilar artery and Willis ring. The pool of brain bottom was dark red. Compared with the control and blank nanoparticles group, the swelling of brain tissue in the nanoparticles and Ropivacaine groups was reduced. Administration of Ropivacaine lactate glycolic acid copolymer nanoparticles into the epidural space of the upper thoracic segment in rabbits can reduce the apoptosis of CVS vascular endothelial cells after SAH.

Download Full-text

Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells

BioMed Research International ◽

10.1155/2013/530712 ◽

2013 ◽

Vol 2013 ◽

pp. 1-13 ◽

Cited By ~ 3

Author(s):

Lin Wang ◽

Chunyan Li ◽

Yingxin Chen ◽

Shujun Dong ◽

Xuesi Chen ◽

...

Keyword(s):

Endothelial Cells ◽

Glycolic Acid ◽

Vascular Endothelial Cells ◽

Umbilical Vein ◽

High Porosity ◽

Vascular Endothelial ◽

Chitosan Microspheres ◽

Expression Levels ◽

Mg63 Cells

Adrenomedullin (ADM) is a bioactive regulatory peptide that affects migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells. This study investigated the effects of sustained release of ADM on the modulation activity of osteoblasts and vascular endothelial cellsin vitro. Chitosan microspheres (CMs) were developed for ADM delivery. Poly(lactic-co-glycolic) acid and nano-hydroxyapatite were used to prepare scaffolds containing microspheres with ADM. The CMs showed rough surface morphology and high porosity, and they were well-distributed. The scaffolds exhibited relatively uniform pore sizes with interconnected pores. The addition of CMs improved the mechanical properties of the scaffolds without affecting their high porosity.In vitrodegradation tests indicated that the addition of CMs increased the water absorption of the scaffolds and inhibited pH decline of phosphate-buffered saline medium. The expression levels of osteogenic-related and angiogenic-related genes were determined in MG63 cells and in human umbilical vein endothelial cells cultured on the scaffolds, respectively. The expression levels of osteogenic-related and angiogenic-related proteins were also detected by western blot analysis. Their expression levels in cells were improved on the ADM delivery scaffolds at a certain time point. Thein vitroevaluation suggests that the microsphere-scaffold system is suitable as a model for bone tissue engineering.

Download Full-text

Effects of Ropivacaine Nanoparticles on the Apoptosis of Cerebral Vascular Endothelial Cells

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.18746 ◽

2020 ◽

Vol 20 (12) ◽

pp. 7299-7304

Author(s):

Duanyu Wang ◽

Yajun Liu ◽

Wei Liu ◽

Haiyan Qi

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Saline Control ◽

Sham Operation ◽

Apoptotic Cells ◽

Vascular Endothelial ◽

Protective Effects ◽

Male Wistar Rats ◽

Ischemic Brain Edema ◽

Capillary Endothelial Cells

To investigate the protective effect of ropivacaine nanoparticles on endothelial cells in the blood brain barrier (BBB) during the development of ischemic brain edema, and its effects on endothelial cell death. Forty-two male Wistar rats weighing 250–300 g and aged 3–4 months were randomly divided into three groups: (1) ropivacaine nanoparticles, (2) saline control and (3) sham operation groups. The membrane of capillary endothelial cells in the animals treated with ropivacaine nanoparticles were intact, with reduced edema, and less severe brain injury when compared to the control. In the ropivacaine nanoparticle group, the number of apoptotic cells decreased at 6 h and 24 h after ischemia, while the number of apoptotic cells in the ischemic penumbra increased. The number of apoptotic cells in the ropivacaine nanoparticles group was significantly lower than in the saline treated control. Ropivacaine nanoparticles exert significant protective effects on the vascular endothelial cells and the BBB during cerebral ischemia.

Download Full-text

Abstract TP267: Plasminogen Inhances the Process of Angiogenesis After Cerebral Ischemia in Mice via Thrombospondin

Stroke ◽

10.1161/str.51.suppl_1.tp267 ◽

2020 ◽

Vol 51 (Suppl_1) ◽

Author(s):

Jinghuan Fang ◽

Michael Chopp ◽

Li Zhang ◽

Fengjie Wang ◽

Zhongwu Liu ◽

...

Keyword(s):

Endothelial Cells ◽

Cerebral Ischemia ◽

Brain Tissue ◽

Vascular Endothelial Cells ◽

Sensory Function ◽

Vascular Endothelial ◽

Middle Cerebral Artery Infarction ◽

Function Test ◽

Angiogenesis Process ◽

Cerebral Vascular

Background: Stroke is the first cause of death in Chinese residents. Plasminogen playes an important role in the process of anigiogenesis. But its specific mechanism of regulating angiogenesis after stroke is not clear. Method: We used wild-type (WT) mice and plasminogen knockout (KO) mice to conduct a permanent middle cerebral artery infarction model (MCAo). At different time points after operation we performed function test; used FITC to measure brain vascular distribution; used western-blot to detect the expression of TSP after MCAo. We used the cerebral vascular endothelial cells for tubeformation. All collected data were processed by corresponding image software, and the results were statistically analyzed by variance analysis or t test. Results: (1) The total length of capillary formation of cerebral vascular endothelial cells in group KO mice was less than that in group WT.(Figure 1) (2) The loss of motor sensory function in group WT was always less than that in group KO. (Figure 2) (3) After MCAo, the blood vessel density in the two groups of mice increased with time, and the density of blood vessels in the WT group was greater than that of the KO group. (Figure 3 ) (4) After MCAo, the levels of TSP-1 and TSP-2 in the cerebral tissue of KO and WT mice were higher than those in the contralateral brain. At the same time, the expression of TSP-1 and TSP-2 in the infarct lateral brain tissue of group KO was higher than that in group WT. (Figure 4) Conclusion: The lack of plasminogen can regulate the expression of TSP-1 and TSP-2 after ischemic stroke, thereby inhibiting the angiogenesis process of brain tissue after cerebral ischemia which leads to poor functional prognosis. stroke.

Download Full-text

M.629 Homocysteine inhibits lysyl oxidase (LOX) and downregulates LOX expression in vascular endothelial cells

Atherosclerosis ◽

10.1016/s0021-9150(04)90627-2 ◽

2004 ◽

Vol 5 (1) ◽

pp. 146

Author(s):

B RAPOSO

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Lysyl Oxidase ◽

Vascular Endothelial

Download Full-text

Anti-metalloproteinase-9 Activities of Selected Indonesian Zingiberaceae Rhizome Extracts in Lipopolysaccharide-induced Human Vascular Endothelial Cells In Vitro

Planta Medica ◽

10.1055/s-0031-1282610 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

Y Yanti ◽

N Steven ◽

A Wiharja ◽

S Fajarianto

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Vascular Endothelial ◽

Metalloproteinase 9

Download Full-text

The Endothelial Cell and the Factor VIII Bypassing Activity of Prothrombin Complex Concentrate

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647034 ◽

1988 ◽

Vol 60 (02) ◽

pp. 226-229 ◽

Cited By ~ 2

Author(s):

Jerome M Teitel ◽

Hong-Yu Ni ◽

John J Freedman ◽

M Bernadette Garvey

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Factor Viii ◽

Prothrombin Complex Concentrate ◽

Vascular Endothelial Cells ◽

Vascular Endothelial ◽

Monolayer Cultures ◽

Coagulant Activity ◽

Time Courses ◽

Privileged Site

SummarySome classical hemophiliacs have a paradoxical hemostatic response to prothrombin complex concentrate (PCC). We hypothesized that vascular endothelial cells (EC) may contribute to this “factor VIII bypassing activity”. When PCC were incubated with suspensions or monolayer cultures of EC, they acquired the ability to partially bypass the defect of factor VIII deficient plasma. This factor VIII bypassing activity distributed with EC and not with the supernatant PCC, and was not a general property of intravascular cells. The effect of PCC was even more dramatic on fixed EC monolayers, which became procoagulant after incubation with PCC. The time courses of association and dissociation of the PCC-derived factor VIII bypassing activity of fixed and viable EC monolayers were both rapid. We conclude that EC may provide a privileged site for sequestration of constituents of PCC which express coagulant activity and which bypass the abnormality of factor VIII deficient plasma.

Download Full-text

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649879 ◽

1995 ◽

Vol 74 (04) ◽

pp. 1045-1049 ◽

Cited By ~ 38

Author(s):

P Butthep ◽

A Bunyaratvej ◽

Y Funahara ◽

H Kitaguchi ◽

S Fucharoen ◽

...

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Immunosorbent Assay ◽

Plasma Proteins ◽

Clinical Symptoms ◽

Vascular Endothelial Cells ◽

Vascular Endothelial Cell ◽

Enzyme Linked Immunosorbent Assay ◽

Vascular Endothelial ◽

Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

Download Full-text