Cerium-oxide-loaded poly(ɛ-caprolactone) hydrogel wound dressing for cutaneous wound healing and antimicrobial treatment in burn wound infection: Development and detailed biological evaluation

2021 ◽  
Vol 11 (12) ◽  
pp. 1966-1974
Author(s):  
Wei Wei ◽  
Liang Xing ◽  
Jianke Feng
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Care Management ◽  
Metal Oxide Nanoparticles ◽  
Healing Process ◽  
Biological Evaluation ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Dressing Materials ◽  
Wound Dressing Materials

Wound dressing materials are essential in wound healing care management. In addition to their supportive effect on the care management, polysaccharides and metal oxide nanoparticles actively contribute to the healing process. CeO2 nanoparticles can promote wound healing through oxidative damage in the wound environment against elevated levels of reactive oxygen species. Therefore, it is necessary to develop multi-functional hydrogel wound dressing materials to stimulate wound healing. In this study, a CeO2-loaded poly(ɛ-caprolactone)/PEC polymeric hydrogel was fabricated as a cutaneous wound-healing material. The in-vitro cytotoxic and wound healing activities were analyzed using the fabricated material in mice. A histological examination showed that the nanofibrous material accelerated the reepithelialization and provided an excellent collagen deposition. In addition, the prepared hydrogels were tested against S. aureus and E. coli. Our results evidence the unique characteristics of the hydrogel wound dressing material exhibiting antibacterial and antioxidant activities and good biocompatibility and healing efficiencies by enhancing the reepithelialization and granulation formation and effectively accelerating the wound healing to prevent skin infections.

scholarly journals Nanotechnology Development for Formulating Essential Oils in Wound Dressing Materials to Promote the Wound-Healing Process: A Review

2021 ◽  
Vol 11 (4) ◽  
pp. 1713
Author(s):  
Ilenia De Luca ◽  
Parisa Pedram ◽  
Arash Moeini ◽  
Pierfrancesco Cerruti ◽  
Gianfranco Peluso ◽  
...  
Keyword(s):  
Wound Healing ◽  
Essential Oils ◽  
Wound Dressing ◽  
Healing Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Efficient Treatment ◽  
Anti Inflammatory ◽  
Dressing Materials ◽  
Wound Dressing Materials

Wound healing refers to the replacement of damaged tissue through strongly coordinated cellular events. The patient’s condition and different types of wounds complicate the already intricate healing process. Conventional wound dressing materials seem to be insufficient to facilitate and support this mechanism. Nanotechnology could provide the physicochemical properties and specific biological responses needed to promote the healing process. For nanoparticulate dressing design, growing interest has focused on natural biopolymers due to their biocompatibility and good adaptability to technological needs. Polysaccharides are the most common natural biopolymers used for wound-healing materials. In particular, alginate and chitosan polymers exhibit intrinsic antibacterial and anti-inflammatory effects, useful for guaranteeing efficient treatment. Recent studies highlight that several natural plant-derived molecules can influence healing stages. In particular, essential oils show excellent antibacterial, antifungal, antioxidant, and anti-inflammatory properties that can be amplified by combining them with nanotechnological strategies. This review summarizes recent studies concerning essential oils as active secondary compounds in polysaccharide-based wound dressings.

A nano zinc oxide doped electrospun scaffold improves wound healing in a rodent model

RSC Advances ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 1428-1439 ◽  
Author(s):  
Hemlata Chhabra ◽  
Rucha Deshpande ◽  
Meghana Kanitkar ◽  
Amit Jaiswal ◽  
Vaijayanti P. Kale ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Care Management ◽  
Wound Care ◽  
Electrospun Scaffold ◽  
Nano Zinc Oxide ◽  
Nano Zinc ◽  
Dressing Materials ◽  
Intense Research ◽  
Wound Dressing Materials

Development of new and effective wound dressing materials continues to be an area of intense research in wound care management. ​Fabricated ZnO doped nanofibrous scaffold exhibited proficiency in EPCs enrichment and wound healing.

Influence of sensory neuropeptides on human cutaneous wound healing process

2014 ◽  
Vol 74 (3) ◽  
pp. 193-203 ◽  
Author(s):  
J. Chéret ◽  
N. Lebonvallet ◽  
V. Buhé ◽  
J.L. Carre ◽  
L. Misery ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Sensory Neuropeptides

scholarly journals Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Zizy I. Elbialy ◽  
Ayman Atiba ◽  
Aml Abdelnaby ◽  
Ibrahim I. Al-Hawary ◽  
Ahmed Elsheshtawy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Rat Model ◽  
Nile Tilapia ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Genes Expression

Abstract Background Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR. Results The histopathological assessment showed noticeable signs of skin healing in TCG compared to CG. Immunohistochemistry results revealed remarkable enhancement in the expression levels of VEGF and TGF-β1 in TCG. Furthermore, TCG exhibited marked upregulation in the VEGF, bFGF, and α-SMA genes expression. These findings suggested that the topical application of Nile tilapia collagen extract can promote the cutaneous wound healing process in rats, which could be attributed to its stimulating effect on recruiting and activating macrophages to produce chemotactic growth factors, fibroblast proliferation, and angiogenesis. Conclusions The collagen extract could, therefore, be a potential biomaterial for cutaneous wound healing therapeutics.

scholarly journals Recombinant Myxoma Virus-Derived Immune Modulator M-T7 Accelerates Cutaneous Wound Healing and Improves Tissue Remodeling

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1003
Author(s):  
Jordan R. Yaron ◽  
Liqiang Zhang ◽  
Qiuyun Guo ◽  
Enkidia A. Awo ◽  
Michelle Burgin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Treatment ◽  
Immune Cell ◽  
Healing Process ◽  
Tissue Remodeling ◽  
Myxoma Virus ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Arginase 1 ◽  
Healing Wounds

Complex dermal wounds represent major medical and financial burdens, especially in the context of comorbidities such as diabetes, infection and advanced age. New approaches to accelerate and improve, or “fine tune” the healing process, so as to improve the quality of cutaneous wound healing and management, are the focus of intense investigation. Here, we investigate the topical application of a recombinant immune modulating protein which inhibits the interactions of chemokines with glycosaminoglycans, reducing damaging or excess inflammation responses in a splinted full-thickness excisional wound model in mice. M-T7 is a 37 kDa-secreted, virus-derived glycoprotein that has demonstrated therapeutic efficacy in numerous animal models of inflammatory immunopathology. Topical treatment with recombinant M-T7 significantly accelerated wound healing when compared to saline treatment alone. Healed wounds exhibited properties of improved tissue remodeling, as determined by collagen maturation. M-T7 treatment accelerated the rate of peri-wound angiogenesis in the healing wounds with increased levels of TNF, VEGF and CD31. The immune cell response after M-T7 treatment was associated with a retention of CCL2 levels, and increased abundances of arginase-1-expressing M2 macrophages and CD4 T cells. Thus, topical treatment with recombinant M-T7 promotes a pro-resolution environment in healing wounds, and has potential as a novel treatment approach for cutaneous tissue repair.

scholarly journals WOUND-HEALING PROPERTIES OF TRANSFORMING GROWTH FACTOR β (TGF-β) INDUCIBLE EARLY GENE 1 (TIEG1) KNOCKOUT MICE

2008 ◽  
Vol 11 (02) ◽  
pp. 63-69 ◽  
Author(s):  
Manabu Taguchi ◽  
Steven L. Moran ◽  
Mark E. Zobitz ◽  
Chunfeng Zhao ◽  
Malayannan Subramaniam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Knockout Mice ◽  
Transforming Growth Factor ◽  
Breaking Strength ◽  
Healing Process ◽  
Early Gene ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Transforming growth factor beta (TGF-β) has a broad effect on wound healing, but many questions remain about the regulation of TGF-β during the healing process. TGF-β inducible early gene 1 (TIEG1) is a primary response gene for TGF-β that controls the activities of the TGF-β/Smad pathway, the primary TGF-β signaling pathway. The purpose of this study was to investigate the role of TIEG1 in cutaneous wound healing using TIEG1 knockout mice. The wound healing in TIEG1 knockout mice and wild-type controls was evaluated by wound breaking strength, Western blot, and histology at postoperative days 3, 7, and 14. Although re-epithelialization of both groups was similarly complete at day 7, the TIEG1 knockout mice had a significantly lower wound breaking strength than the controls at postoperative day 14. These results suggest that TIEG1 expression may be an important factor involved in the initiation and support of normal cutaneous wound healing.

scholarly journals Temporal Effects of Topical Morphine Application on Cutaneous Wound Healing

2008 ◽  
Vol 109 (1) ◽  
pp. 130-136 ◽  
Author(s):  
Jerri M. Rook ◽  
Wohaib Hasan ◽  
Kenneth E. McCarson
Keyword(s):  
Wound Healing ◽  
Time Course ◽  
Wound Closure ◽  
Healing Process ◽  
Topical Administration ◽  
Scar Tissue ◽  
Skin Thickness ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Opioid Administration

Background Studies have shown that topical administration of exogenous opioid drugs impairs wound healing by inhibiting the peripheral release of neuropeptides, thereby inhibiting neurogenic inflammation. This delay is immediate and peaks during the first days of wound closure. This study examined the effects of topical morphine treatment in a cutaneous wound healing model in the rat. Methods Full-thickness 4-mm-diameter wounds were placed on the periscapular region of rats that subsequently received twice-daily topical applications of IntraSite Gel (Smith+Nephew, Hull, United Kingdom) alone or gel infused with 5 mm morphine sulfate on days 0-3 or 4-10 postwounding or throughout the time course. Wound tissue was taken on days 1, 3, 5, 8, and 18 postwounding and immunostained for myofibroblast and macrophage markers or stained with hematoxylin and eosin. Results Delays in wound closure observed during morphine application on days 0-3 postwounding mimicked those seen in wounds treated with morphine throughout the entire healing process. However, no significant delays in closure were seen in wounds treated with morphine beginning on day 4 postwounding. Treatment of wounds with morphine significantly reduced the number of myofibroblasts and macrophages in the closing wound. In addition, morphine application resulted in decreases in skin thickness and an increase in residual scar tissue in healed skin. Conclusions These findings demonstrate the time-dependent and persistent nature of the detrimental effects of topical morphine on cutaneous wound healing. The data identify specific limitations that could be ameliorated to optimize topical opioid administration as an analgesic therapeutic strategy in the treatment of painful cutaneous wounds.

scholarly journals Stem Cells for Cutaneous Wound Healing

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Giles T. S. Kirby ◽  
Stuart J. Mills ◽  
Allison J. Cowin ◽  
Louise E. Smith
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Clinical Use ◽  
Cutaneous Wound Healing ◽  
Cell Therapies ◽  
Cutaneous Wound ◽  
Matrix Deposition ◽  
Obesity And Diabetes

Optimum healing of a cutaneous wound involves a well-orchestrated cascade of biological and molecular processes involving cell migration, proliferation, extracellular matrix deposition, and remodelling. When the normal biological process fails for any reason, this healing process can stall resulting in chronic wounds. Wounds are a growing clinical burden on healthcare systems and with an aging population as well as increasing incidences of obesity and diabetes, this problem is set to increase. Cell therapies may be the solution. A range of cell based approaches have begun to cross the rift from bench to bedside and the supporting data suggests that the appropriate administration of stem cells can accelerate wound healing. This review examines the main cell types explored for cutaneous wound healing with a focus on clinical use. The literature overwhelmingly suggests that cell therapies can help to heal cutaneous wounds when used appropriately but we are at risk of clinical use outpacing the evidence. There is a need, now more than ever, for standardised methods of cell characterisation and delivery, as well as randomised clinical trials.

scholarly journals Advances of Stem Cell Therapeutics in Cutaneous Wound Healing and Regeneration

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Suman Kanji ◽  
Hiranmoy Das
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Healing Process ◽  
Treatment Modalities ◽  
Wound Healing Process ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Cutaneous wound healing is a complex multiple phase process, which overlaps each other, where several growth factors, cytokines, chemokines, and various cells interact in a well-orchestrated manner. However, an imbalance in any of these phases and factors may lead to disruption in harmony of normal wound healing process, resulting in transformation towards chronic nonhealing wounds and abnormal scar formation. Although various therapeutic interventions are available to treat chronic wounds, current wound-care has met with limited success. Progenitor stem cells possess potential therapeutic ability to overcome limitations of the present treatments as it offers accelerated wound repair with tissue regeneration. A substantial number of stem cell therapies for cutaneous wounds are currently under development as a result of encouraging preliminary findings in both preclinical and clinical studies. However, the mechanisms by which these stem cells contribute to the healing process have yet to be elucidated. In this review, we emphasize on the major treatment modalities currently available for the treatment of the wound, role of various interstitial stem cells and exogenous adult stem cells in cutaneous wound healing, and possible mechanisms involved in the healing process.

Sign in / Sign up

Export Citation Format

Share Document