Osthole Reduces Mouse IOP Associated With Ameliorating Extracellular Matrix Expression of Trabecular Meshwork Cell

In primary open angle glaucoma, decrease of hyaluronic acid level is related to extracellular matrix deposition of trabecular meshwork. Extracellular matrix deposition will increase humor aqueous outflow resistance which in turn increase the intraocular pressure. MMP-9 as one of matrix metalloproteinase (MMP) family, has a high potency to degrade the extracellular matrix. The expression of MMP-9 can be activated by hyaluronic acid through its binding with CD44 as hyaluronic main receptor. The aim of this research is to determine the effect of hyaluronic acid on MMP-9 expression in trabecular meshwork cell culture of patient with primary open angle glaucoma by immunocytochemical method. Trabecular meshwork cell culture were exposed to hyaluronic acid at four different concentration (0mg/ml, 1 mg/mL, 3 mg/mL, 6 mg/mL). After 24 hours incubated, we find a significant difference of MMP-9 expression between control group and group with hyaluronic acid. There was significant correlation between hyaluronic acid and MMP-9 expression. Hyaluronic acid exposure will increase MMP-9 expression in trabecular meshwork cell culture. This finding suggest that hyaluronic acid can influence the resistance of humor aqueous outflow in trabecular meshwork by up-regulating MMP-9 expression, and therefore will increase extracellular matrix degradation.Key words : hyaluronic acid, primary open angle glaucoma, MMP-9

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Canonical Wnt Signaling Regulates Extracellular Matrix Expression in the Trabecular Meshwork

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.13-12652 ◽

2014 ◽

Vol 55 (11) ◽

pp. 7433 ◽

Cited By ~ 23

Author(s):

Guadalupe Villarreal ◽

Ayan Chatterjee ◽

Sarah S. Oh ◽

Dong-Jin Oh ◽

Min Hyung Kang ◽

...

Keyword(s):

Extracellular Matrix ◽

Wnt Signaling ◽

Trabecular Meshwork ◽

Canonical Wnt Signaling ◽

Matrix Expression ◽

Canonical Wnt

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Circumferential trabecular meshwork cell density in the human eye

Experimental Eye Research ◽

10.1016/j.exer.2021.108494 ◽

2021 ◽

Vol 205 ◽

pp. 108494

Author(s):

Markus H. Kuehn ◽

Janice A. Vranka ◽

David Wadkins ◽

Thomas Jackson ◽

Lin Cheng ◽

...

Keyword(s):

Cell Density ◽

Trabecular Meshwork ◽

Human Eye ◽

Trabecular Meshwork Cell

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Effect of dexamethasone on the expression of MMPs, adenosine A1 receptors and NFKB by human trabecular meshwork cells

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2019-0373 ◽

2020 ◽

Vol 0 (0) ◽

Cited By ~ 1

Author(s):

Normie Aida Mohd Nasir ◽

Renu Agarwal ◽

Anna Krasilnikova ◽

Siti Hamimah Sheikh Abdul Kadir ◽

Igor Iezhitsa

Keyword(s):

Extracellular Matrix ◽

Western Blot ◽

Trabecular Meshwork ◽

Culture Media ◽

Smooth Muscle Actin ◽

Adenosine A1 Receptors ◽

Trabecular Meshwork Cells ◽

Aqueous Humor Dynamics ◽

Nfkb Activation ◽

Adenosine A1

AbstractObjectivesSteroid-induced ocular hypertension and glaucoma are associated with extracellular matrix remodeling at the trabecular meshwork (TM) of the eye due to reduced secretion of matrix metalloproteinases (MMPs), a family of enzymes regulating extracellular matrix proteolysis. Several biological functions of steroids are known to involve regulation of adenosine A1 receptors (A1AR) and nuclear factor kappa B (NFKB). Since MMPs expression in TM has been shown to be regulated by A1AR as well as transcription factors, it is likely that dexamethasone-induced changes in aqueous humor dynamics involve reduced MMP and A1AR expression and reduced NFKB activation. Hence, the current study investigated the association of dexamethasone-induced reduction in MMP secretion with reduced NFKB activation and A1AR expression.MethodsHuman trabecular meshwork cells (HTMCs) were characterized by estimating myocilin and alpha smooth muscle actin expression and then were treated with dexamethasone 100 nM for 2, 5 and 7 days. The MMP secretion was estimated in culture media using Western blot. Immunocytochemistry (ICC) and ELISA were done to investigate the effect of dexamethasone on NFKB phosphorylation. A1AR expression in HTMCs was determined using Western blot and ELISA.ResultsDexamethasone caused a significant reduction in both MMP-2 and -9 expression compared to untreated group after five and seven days but not after two days of culture. Significantly reduced phosphorylated NFKB and A1AR protein levels were detected in dexamethasone treated compared to vehicle treated HTMCs after five days of culture.ConclusionsDexamethasone reduces MMP-2 and -9 secretion by HTMCs and this effect of dexamethasone is associated with reduced NFKB phosphorylation and A1AR expression.

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