Electron Microscopy in the Diagnosis of Liposarcoma and Fibrosarcoma of the Larynx

1979 ◽  
Vol 88 (6) ◽  
pp. 867-871 ◽  
Author(s):  
David N. Tobey ◽  
Roger F. Wheelis ◽  
C. Thomas Yarington
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Accurate Determination ◽  
Final Diagnosis ◽  
Primary Lesion ◽  
Low Grade ◽  
Pathologic Evaluation ◽  
First Case ◽  
Per Se

Electron microscopy (EM) was useful in establishing the final diagnosis in specific instances of liposarcoma and fibrosarcoma of the larynx. In the first case light microscopy revealed cells that met the histologic criteria of a low grade liposarcoma; EM revealed cells that displayed a varied ultrastructural differentiation; thus the tumor was classified as a mixed mesenchymal tumor rather than liposarcoma per se. In the second case, pathologic evaluation indicated a grade 2 fibrosarcoma. EM revealed malignant mesenchymal cells that closely recapitulated normal fibroblasts (no epithelial differentiation was present). This kind of information enables more accurate determination of the source of the primary lesion than results of light microscopy alone.

scholarly journals The Significance of Death-Scene Investigation in Determining Cause and Circumstances of Medico-Legal Death

2019 ◽  
Vol 24 (38) ◽  
pp. 53-62
Author(s):  
Corina Crișan
Keyword(s):  
Accurate Determination ◽  
Massive Hemoptysis ◽  
Death Scene ◽  
First Case ◽  
Death Scene Investigation ◽  
Traumatic Lesions ◽  
Scene Investigation ◽  
Mechanical Asphyxia

Abstract The article presents two cases of the Arad Medico-Legal Department illustrating just a part of the role of the forensic pathologist at the death scene but there are sufficient to fully justify the importance of this investigation as no example can comprise the complexity of problems and the particularity of each case, nor a statistic can be made. Both cases were found dead at home and forensically autopsied, but the two of them were distinct in terms of forensic pathologist's request death scene participation. In the first case, the autopsy did not find traumatic lesions, but revealed that the death was due to massive hemoptysis caused by cavernous tuberculosis with subsequent exsanguination, microscopically confirmed. The death was nonviolent. In the second case, the autopsy revealed findings of mechanical asphyxia due to neck compression, both macroscopically and microscopically. The death was violent. In both cases the forensic expert participation is required at the death-scene. In the first case it allowed the correct interpretation of the traces of blood found on site, and in the second case, an onsite research would have properly helped for restoring the death‟s occurrence. The scene investigation and autopsy provide, together, the basis for an accurate determination of cause and circumstances of death.

scholarly journals Spinal Intradural, Extramedullary Ependymoma with Astrocytoma Component: A Case Report and Review of the Literature

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Gene M. Weinstein ◽  
Knarik Arkun ◽  
James Kryzanski ◽  
Michael Lanfranchi ◽  
Gaurav K. Gupta ◽  
...  
Keyword(s):  
Back Pain ◽  
Case Report ◽  
Lower Back Pain ◽  
Low Grade ◽  
Review Of The Literature ◽  
Pathologic Evaluation ◽  
Lower Back ◽  
Spinal Lesions ◽  
First Case ◽  
Intradural Extramedullary

Ependymomas are common spinal lesions, with the vast majority arising in an intramedullary location. Several cases have been described in the literature of ependymomas in an intradural, extramedullary location. The authors present a case of a 56-year-old female who presented with several weeks of lower back pain and weakness. MRI revealed an intradural, extramedullary enhancing mass at L1-L2. The mass was successfully resected surgically. Pathologic evaluation revealed a low grade glioma with components of both ependymoma and pilocytic astrocytoma with MUTYH G382D mutation. Extramedullary ependymomas are very rare tumors. To the authors’ knowledge, this is the first case of ependymoma/astrocytoma collision tumors described in an extramedullary location.

scholarly journals Porous or non-porous? The challenge of studying unusual placoid sensilla of Megaphragma wasps (Hymenoptera, Trichogrammatidae) with electron microscopy

2021 ◽  
Vol 84 ◽  
pp. 69-73
Author(s):  
Anna V. Diakova ◽  
Alexey A. Polilov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Accurate Determination ◽  
Apparent Absence ◽  
Olfactory Sensilla ◽  
High Degree ◽  
Scanning Electron

Extreme miniaturization implies a high degree of optimization, rendering the retention of non-functional organs almost impossible. Two unique non-porous placoid sensilla on the antennae of females of Megaphragma were described in the literature. Placoid sensilla in Hymenoptera have an olfactory function and always bear pores; the apparent absence of pores therefore raises the questions whether such sensilla are functional in Megaphragma and whether their surface sculpture had been sufficiently well examined. We examined in detail the external microsculpture and internal ultrastructure of the placoid sensilla using Focused Ion Beam Scanning Electron Microscopy and Scanning Electron Microscopy with various types of sputtering and show that these sensilla actually have a porous cuticle and are innervated by 11 or 12 neurons with branched cilia, which is typical of olfactory sensilla. Comparison of various methods of electron microscopy allows us to conclude that for an accurate determination of the morphofunctional types of sensilla, especially in miniature insects, it is necessary to study both the internal ultrastructure of the sensilla and their external morphology using carefully selected scanning electron microscopy methods.

scholarly journals A reevaluation of routine electron microscopy in the examination of native renal biopsies.

1997 ◽  
Vol 8 (1) ◽  
pp. 70-76
Author(s):  
M Haas
Keyword(s):  
Electron Microscopy ◽  
Lupus Nephritis ◽  
Light Microscopy ◽  
Final Diagnosis ◽  
Preliminary Diagnosis ◽  
Ultrastructural Studies ◽  
Collapsing Glomerulopathy ◽  
Postinfectious Glomerulonephritis ◽  
Renal Biopsies ◽  
Made In

Electron microscopy is routinely utilized in most centers in the evaluation of native renal biopsies. Several studies, primarily from the 1960s and early 1970s, provide justification for its use. Conducted by Siegel et al. (1), the largest study evaluated 213 consecutive renal biopsies and found that electron microscopy was needed for a correct diagnosis in 11%, as well as for confirmation or additional information in another 36%. However, nearly all of these studies were conducted before the use of immunofluorescence in renal biopsy diagnosis became widespread and before several new glomerular diseases and variants were described. In light of this situation and the expense of the procedure, the routine use of electron microscopy in native renal biopsies also examined by immunofluorescence and routine light microscopy was reevaluated. From January 1996 to June 1996, 288 native renal biopsies were received, and all were evaluated by the same pathologist. Of those, 233 met criteria for inclusion in this study, which were > or = 5 glomeruli for light microscopy, > or = 2 for immunofluorescence, and > or = 1 for electron microscopy, not including globally scarred glomeruli. Light microscopy (hematoxylin and eosin, periodic acid-Schiff stains) and immunofluorescence--for immunoglobulin (Ig) G, IgA, IgM, C3, C1q, fibrinogen; kappa/lambda when needed--were evaluated on each biopsy within 48 h of receipt, and a preliminary diagnosis was recorded if possible. Electron microscopy was then performed, and a final diagnosis was made. In 50 cases (21%), electron microscopy was needed to make the final diagnosis; in two of these cases, the preliminary diagnosis was incorrect, and in 48, a firm preliminary diagnosis could not be made. In the other cases, the preliminary diagnosis was correct, but in 48 (21%), ultrastructural study was felt to provide important confirmatory data, and in eight cases (3%), an additional, unrelated diagnosis was supported by the ultrastructural findings. Diagnoses most frequently requiring electron microscopy included minimal change nephropathy, early diabetic nephropathy, membranous lupus nephritis, membranoproliferative glomerulonephritis, postinfectious glomerulonephritis, thin basement membrane nephropathy (or exclusion of this in cases of otherwise unexplained hematuria), and human immunodeficiency virus-associated nephropathy (or exclusion of it in cases of collapsing glomerulopathy). Common diagnoses usually not requiring electron microscopy included IgA nephropathy, diffuse proliferative lupus nephritis, focal segmental glomerulosclerosis (not collapsing glomerulopathy variant), pauci-immune crescentic glomerulonephritis, acute interstitial nephritis, and amyloid nephropathy. This study confirms that, as was the case 20 to 30 yr ago, electron microscopy provides useful diagnostic information in nearly half of native renal biopsies. If electron microscopy cannot be performed routinely on all such biopsies, it is recommended that tissue for ultrastructural studies be set aside in each case.

Change of Arsenic Coverage on GaAs(001)

1993 ◽  
Vol 312 ◽  
Author(s):  
Holger Nörenberg ◽  
Nobuyuki Koguchi
Keyword(s):  
Electron Microscopy ◽  
Molecular Beam ◽  
Accurate Determination ◽  
High Energy ◽  
Surface Structures ◽  
Rheed Pattern ◽  
High Energy Electron ◽  
Reconstructed Surface ◽  
Scanning Electron

Abstract(2×4) and c(4×4) reconstructed GaAs(001) surfaces prepared by Molecular Beam Epitaxy (MBE) were studied by Reflection High Energy Electron Diffraction (RHEED). A method for accurate determination of the Arsenic coverage of reconstructed GaAs(001) surfaces is introduced. The time of Gallium supply to the reconstructed surface until a halo appears in the RHEED pattern is taken as measure for the Arsenic coverage. Structures between them were investigated at a substrate temperature of 200°C. The RHEED results were verified by High Resolution Scanning Electron Microscopy (HRSEM). Dependent on the surface reconstruction, Arsenic coverages between 0.76 and 1.22 monolayer (ML) were observed. Surface structures, observed during transformation between β(2×4) and c(4×4) will be discussed.

scholarly journals Spina Bifida—Ultrasonographic Diagnosis in First and Second Trimesters

2017 ◽  
Vol 11 (4) ◽  
pp. 341-346 ◽  
Author(s):  
Laura Sarno ◽  
Gabriele Saccone ◽  
Marco Di Cresce ◽  
Pasquale Martinelli ◽  
Giuseppe Maria Maruotti
Keyword(s):  
Spina Bifida ◽  
Accurate Determination ◽  
Three Dimensional ◽  
Fetal Surgery ◽  
Final Diagnosis ◽  
3D Ultrasound ◽  
Ultrasonographic Diagnosis ◽  
Myelomeningocele Repair ◽  
Location Type

ABSTRACT Accurate and timely prenatal diagnosis of spina bifida (SB) is a major challenge of actual antenatal care. The diagnosis of spina bifida may be only suspected during I trimester because the detection rate of intracranial traslucency is 50%; the final diagnosis is made in the II trimester by direct visualization of defect of spine or the presence of myelomeningocele or the visualization of indirect signs. When a spine defect is detected it is necessary a neurosurgical counseling with the patient .The degree of handicap and the survival rate depend on the level of injury, the size of the defect, and the presence of associated anomalies. In tertiary fetal medicine centers, two-dimensional (2D) and three-dimensional (3D) ultrasound allows an accurate determination of the location, type, extention of the defect. Maternal—fetal surgery for myelomeningocele repair must be offered to carefully selected patients even if there are significant maternal implications and complications How to cite this article Sarno L, Saccone G, Di Cresce M, Martinelli P, Maruotti GM. Spina Bifida—Ultrasonographic Diagnosis in First and Second Trimesters. Donald School J Ultrasound Obstet Gynecol 2017;11(4):341-346.

scholarly journals The role of electron microscopy for the diagnosis of glomerulopathies

2004 ◽  
Vol 122 (3) ◽  
pp. 104-109 ◽  
Author(s):  
Angelo Sementilli ◽  
Luiz Antonio Moura ◽  
Marcello Fabiano Franco
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Laboratory Data ◽  
Final Diagnosis ◽  
Glomerular Diseases ◽  
Financial Pressure ◽  
Renal Biopsies ◽  
Morphological Diagnosis ◽  
Selection Of

CONTEXT: Electron microscopy has been used for the morphological diagnosis of glomerular diseases for more than three decades and its value has been widely emphasized. However, recent reports have analyzed the routine use of electron microscopy critically. Its use in other areas of diagnosis such as tumor diseases has declined considerably; in addition, in view of the unavoidable financial pressure for the reduction of costs due to investigations and diagnostic routines, the selection of cases for electron microscopy has been quite rigorous. OBJECTIVE: To identify the glomerular diseases that depend on electron microscopy for a final diagnosis, by means of reviewing renal biopsies performed over a 12-year period. DESIGN: Prospective SETTING: Hospital Ana Costa, Hospital Guilherme Álvaro and Serviço de Anatomia Patológica de Santos, Santos, São Paulo, Brazil. PARTICIPANTS: 200 consecutive renal biopsies obtained from private hospitals and the teaching hospital from 1979 to 1991 were studied. MAIN MEASUREMENTS: All cases were analyzed via light microscopy, immunofluorescence and electron microscopy. The diagnosis was first made via light microscopy plus immunofluorescence and then via electron microscopy. RESULTS: Electron microscopy was diagnostic or essential for diagnosis in 10.0% of the cases, corresponding to 3.4% of primary glomerulopathies and 100% of hereditary glomerulopathies. Electron microscopy was contributory (useful) to the diagnosis in 5.5% of the cases, confirming the preliminary diagnosis formulated on the basis of clinical and laboratory data and light microscopy plus immunofluorescence findings. We obtained a 7.5% rate of discordant immunofluorescence, which was considered as such when negative immunofluorescence findings were not confirmed by electron microscopy. The final diagnosis with the use of light microscopy plus immunofluorescence alone was 77.0%. CONCLUSIONS: It was possible to diagnose with certainty a great percentage of glomerulopathies (82.5-90% of the cases) based on the light microscopy and immunofluorescence findings alone. Electron microscopy was essential for the diagnosis of hereditary nephropathies.

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