Treatment of Surface Plasmon Resonance (SPR) Background in Total Internal Reflection Ellipsometry: Characterization of RNA Polymerase II Film Formation

2019 ◽  
Vol 73 (3) ◽  
pp. 261-270
Author(s):  
Dušan Hemzal ◽  
Yu Ri Kang ◽  
Jan Dvořák ◽  
Tomasz Kabzinski ◽  
Karel Kubíček ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Film Growth ◽  
Difference Spectra ◽  
The Difference ◽  
Total Internal Reflection Ellipsometry

To deal with the general problem of biomolecule specific binding analysis, we have applied the technique of difference spectra to the surface plasmon resonance (SPR)-enhanced total internal reflection ellipsometry measurement. We suggest a three-step treatment of the SPR background that can easily be integrated with the usual measurement routine. First, making use of the difference spectrum in ellipsometric angle Δ, single peak footprints of the topmost layer are obtained that facilitate its sensitive detection during film growth. Subsequently, circumventing the need for explicit knowledge of the substrate properties, the difference spectra peaks can be used for the end-point analysis of a binding. Finally, tracking the binding effectivity of the analyte we determine the injection speed and analyte concentration windows needed for successful monitoring of the film growth. We demonstrate our approach on a comprehensive two-stage binding experiment involving two biologically relevant molecules: the C-terminal domain (CTD) of RNA polymerase II and CTD-interacting domain of one of its transcription factors, the Rtt103 protein.

scholarly journals Optimization of The Mechanical Drive on Set-up of Homemade Surface Plasmon Resonance

2017 ◽  
Vol 1 (2) ◽  
pp. 108
Author(s):  
Muhammad Arifin ◽  
Febrilian Dwi Laksono ◽  
Supardianningsih Supardianningsih ◽  
Kamsul Abraha
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Incident Angle ◽  
Gold Thin Film ◽  
The Difference ◽  
Set Up ◽  
Mechanical Optimization

Mechanical optimization has been done on the surface plasmon resonance (SPR) homemade. At set-up, angle scanning comes a small resolution to detect changes in the thickness of the thin film or refractive index of the sample. The mechanical drive has a function to rotate the prism and detector. Torsional Spring-Loaded Gear Antibacklash is used to improve the accuracy of the mechanical drive. The stepper motor is used to rotate the mechanical drive automatically. The built system has successfully increased the resolution of the prism rotation angle by 0.01°. The test using the blank prism indicates the angle of Total Internal Reflection occurs at an incident angle of 41.80 °. The SPR phenomenon was observed in a thin layer of gold deposited on the prism using a vacuum evaporator. The observed SPR angles on samples A, B, C, D, E, F, G, H and I were 45.02°, 45.05°, 46.30°, 44.48°, 44.70°, 45.49°, 45.52°, 45.66°, and 44.44°, respectively. The SPR angle change shows the difference in the thickness of the gold thin film.

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

The Revised Method of Quantitative Detection of Animal-Origin Bovine and Porcine Gelatin Difference Using Surface Plasmon Resonance Based Biosensor

2019 ◽  
Vol 948 ◽  
pp. 146-152
Author(s):  
Devy Pramudyah Wardani ◽  
Muhammad Arifin ◽  
Kamsul Abraha
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Sensor Response ◽  
Animal Origin ◽  
Quantitative Detection ◽  
Response Models ◽  
Gelatin Concentration ◽  
The Difference ◽  
Different Characteristics

We have previously reported the surface plasmon resonance (SPR)-based biosensor ability for quantitatively differentiating bovine and porcine gelatin has been done by us before. However, it has some inaccuracies. By improving the method of detection, the results of this study shows that the difference between bovine and porcine gelatin was more distinguishable. The sensor response models acquired were nonlinear as in the previous study. However, they show different characteristics. The sensitivities of the sensor obtained are higher than those of the previous ones, i.e., 3.04o and 4.29o for bovine and porcine gelatin concentration change of 0.1%, respectively. And the sensor’s LOD and LOQ towards both gelatin concentrations were 0.22% and 0.74% (w/w), respectively.

In Situ Observation of Formation Behavior of Adsorption Film in the Narrow Space Using Surface Plasmon Resonance (SPR) Method

2017 ◽  
Vol 739 ◽  
pp. 56-62
Author(s):  
Koki Fukuta ◽  
Satoru Maegawa ◽  
Fumihiro Itoigawa ◽  
Takashi Nakamura
Keyword(s):  
Surface Plasmon Resonance ◽  
Film Thickness ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Film Growth ◽  
High Sensitivity ◽  
Adsorption Film ◽  
Base Oil

In this paper, an application of the surface plasmon resonance (SPR) method to real-time and in-situ measurements of changes in adsorption film thickness, e.g., fatty acid adsorbed films, is presented. As discussed in the previous study, the high sensitivity and overall simplicity of its optical system make the SPR method advantageous for monitoring lubricant film thickness. In order to demonstrate the high sensitivity of the SPR method, in-situ and real-time adsorption film growth processes of stearic acid (additives) in hexadecane (base oil) were monitored using the Otto configuration. Based on a comparison between experimental and model calculation results, changes in the adsorption film thickness during a series of different contact tests were measured.

scholarly journals Template-Free and Surfactant-Free Synthesis of Selective Multi-Oxide-Coated Ag Nanowires Enabling Tunable Surface Plasmon Resonance

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1949
Author(s):  
Chi-Hang Tsai ◽  
Shih-Yun Chen ◽  
Alexandre Gloter ◽  
Jenn-Ming Song
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Wavelength ◽  
Oxide Thickness ◽  
Refractive Indices ◽  
Surface Plasma Resonance ◽  
Titania Layer ◽  
Ag Nanowires ◽  
The Difference

Without using templates, seeds and surfactants, this study successfully prepared multi-oxide-layer coated Ag nanowires that enable tunable surface plasmon resonance without size or shape changes. A spontaneously grown ultra-thin titania layer onto the Ag nanowire surface causes a shift in surface plasmon resonance towards low energy (high wavelength) and also acts as a preferential site for the subsequent deposition of various oxides, e.g., TiO2 and CeO2. The difference in refractive indices results in further plasmonic resonance shifts. This verifies that the surface plasma resonance wavelength of one-dimensional nanostructures can be adjusted using refractive indices and shell oxide thickness design.

Sign in / Sign up

Export Citation Format

Share Document