EXPRESS: Evaluation of the quick-clotting serum separator tube, VQ-TubeTM, for clinical chemistry and thyroid hormone assays

2021 ◽  
pp. 000456322110182
Author(s):  
Sung Jin Jo ◽  
Hyo-jin Chae ◽  
Yong-Wha Lee ◽  
Jong Do Seo ◽  
Sang Hoon Song ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
University Hospitals ◽  
Laboratory Results ◽  
Collection Tube ◽  
Specimen Quality ◽  
Gel Barrier ◽  
Blood Collection Tube

Background: The type of blood collection tube affects specimen quality and laboratory results. Because plasma specimens have a shorter processing time compared to serum specimens, emergency biochemistry tests use plasma. However, serum specimens remain stable after centrifugation and show more accurate results than plasma. Therefore, a quick-clotting serum separator tube (SST) is expected to be useful for shorter turnaround times and accurate results. We evaluated a new quick-clotting SST VQ-Tube<sup>TM</sup> (AB Medical, Korea) for clinical chemistry and thyroid hormone assays. Methods: One hundred volunteers from four university hospitals were recruited and peripheral blood samples were collected in quick-clotting SST VQ-Tubes<sup>TM</sup> and the commonly used SST V-Tubes<sup>TM</sup>. The obtained specimens were used for 16 clinical chemistry assays and three thyroid hormone assays. Results: The differences (%) in the test results obtained from the samples in each tube satisfied the allowable difference ranges (19 assays). The differences in the test results between the tubes satisfied the desired specifications for accuracy except for the glucose results (2.75%). The paired t-test revealed significant differences between the results of six assays but each set of results showed a good correlation. Samples were visually inspected for serum clarity and gel barrier integrity, and incomplete clotting reactions and hemolyzed serum were not observed. Conclusion: The new quick-clotting VQ-Tube<sup>TM</sup> demonstrated reliable test results compared to the commonly used SST V-Tube<sup>TM</sup>. This quick-clotting tube will provide fast test results with adequately separated serum specimens, especially for patients who need fast tests.

A Multicenter Evaluation of a Nongel Mechanical Separator Plasma Blood Collection Tube for Testing of Selected Therapeutic Drugs

2020 ◽  
Vol 5 (4) ◽  
pp. 671-685
Author(s):  
Svetlana Morosyuk ◽  
Julie Berube ◽  
Robert Christenson ◽  
Alan H B Wu ◽  
Denise Uettwiller-Geiger ◽  
...  
Keyword(s):  
The Other ◽  
Initial Time ◽  
Therapeutic Drug ◽  
Blood Collection ◽  
Test Results ◽  
Sample Storage ◽  
Therapeutic Drugs ◽  
Collection Tube ◽  
And Storage ◽  
Blood Collection Tube

Abstract Background Some therapeutic drugs are unstable during sample storage in gel tubes. BD Vacutainer® Barricor™ Plasma Blood Collection Tube with nongel separator was compared with plasma gel tubes, BD Vacutainer PST™, PST II, and BD Vacutainer Serum Tube for acetaminophen, salicylate, digoxin, carbamazepine, phenytoin, valproic acid, and vancomycin during sample storage for up to 7 days. Methods Seven hospital sites enrolled 705 participants who were taking at least one selected drug. The study tubes were collected and tested at initial time (0 h), after 48 h of storage at room temperature and on day 7 (after additional 5 days of refrigerated storage). The performance of BD Barricor tube was evaluated for each drug by comparing BD Barricor samples with samples from the other tubes at 0 h from the same participant; stability was evaluated by comparing test results from the same tube at 0 h, 48 h, and 7 days. Results At 0 h, BD Barricor showed clinically equivalent results for selected therapeutic drugs compared with the other tubes, except phenytoin in BD PST. Phenytoin samples ≥20 µg/mL in BD PST had 10–12% lower values than samples in BD Barricor. During sample storage, all selected drugs remained stable for 7 days in BD Barricor and in serum aliquots. In BD PST, all drugs remained stable except phenytoin and carbamazepine and in BD PST II except for phenytoin. Conclusion The BD Barricor Tube is effective for the collection and storage of plasma blood samples for therapeutic drug monitoring without sample aliquoting.

scholarly journals Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1019
Author(s):  
Kyungjin Hong ◽  
Gabriella Iacovetti ◽  
Ali Rahimian ◽  
Sean Hong ◽  
Jon Epperson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
Sample Collection ◽  
Rapid Separation ◽  
Cell Counts ◽  
Collection Device ◽  
Precision Study ◽  
Clinically Significant ◽  
Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

scholarly journals Interlaboratory comparison of the TransFix®/EDTA Vacuum Blood Collection tube with the 5 mL Cyto‐Chex® BCT

2018 ◽  
Vol 96 (6) ◽  
pp. 496-507 ◽  
Author(s):  
Daniel Harrison ◽  
Rosalie Ward ◽  
Sarah Bastow ◽  
Andrew Parr ◽  
Susan Macro ◽  
...  
Keyword(s):  
Interlaboratory Comparison ◽  
Blood Collection ◽  
Collection Tube ◽  
Blood Collection Tube

Residual Negative Pressure in Vacuum Blood-Collection Tube and Hemolysis in Pediatric Blood Specimens

2019 ◽  
Vol 51 (1) ◽  
pp. 41-46
Author(s):  
Jing Hu ◽  
Qiao-Xin Zhang ◽  
Tong-Tong Xiao ◽  
Mei-Chen Pan ◽  
Ying-Mu Cai
Keyword(s):  
Negative Pressure ◽  
Blood Collection ◽  
Free Hemoglobin ◽  
Significant Difference ◽  
Collection Tube ◽  
Biochemical Analyzer ◽  
Blood Specimens ◽  
Adult Volunteers ◽  
Blood Collection Tubes ◽  
Blood Collection Tube

ABSTRACT Objective To determine a method to reduce specimen hemolysis rates in pediatric blood specimens. Methods A total of 290 blood specimens from pediatric patients were classified into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer. Also, we performed a paired test to measure the concentration of free hemoglobin in specimens from 25 randomly selected healthy adult volunteers, using a direct spectrophotometric technique. Results The hemolytic rate of capped specimens was 2-fold higher than that of uncapped specimens. We found significant differences for LDH. Also, there was a significant difference in the concentration of free hemoglobin in the random-volunteers test. Conclusions Eliminating the residual negative pressure of vacuum blood-collection tubes was effective at reducing the macrohemolysis and/or microhemolysis rate.

Non-invasive fetal RHD genotyping for RhD negative women stratified into RHD gene deletion or variant groups: comparative accuracy using two blood collection tube types

Pathology ◽  
2017 ◽  
Vol 49 (7) ◽  
pp. 757-764 ◽  
Author(s):  
Catherine A. Hyland ◽  
Glenda M. Millard ◽  
Helen O'Brien ◽  
Elizna M. Schoeman ◽  
Genghis H. Lopez ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Blood Collection ◽  
Non Invasive ◽  
Collection Tube ◽  
Comparative Accuracy ◽  
Blood Collection Tube

scholarly journals It's Time for a Better Blood Collection Tube to Improve the Reliability of Glucose Results

Diabetes Care ◽  
2012 ◽  
Vol 36 (1) ◽  
pp. e2-e2 ◽  
Author(s):  
M. J. Peake ◽  
D. E. Bruns ◽  
D. B. Sacks ◽  
A. R. Horvath
Keyword(s):  
Blood Collection ◽  
Collection Tube ◽  
Blood Collection Tube
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