The Influence of Sucralose on Bacterial Metabolism

1990 ◽  
Vol 69 (8) ◽  
pp. 1480-1484 ◽  
Author(s):  
D.A. Young ◽  
W.H. Bowen
Keyword(s):  
Agar Medium ◽  
Sucrose Concentration ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Streptococcus Sobrinus ◽  
Streptococcus Sanguis ◽  
Inhibition Of Growth ◽  
Total Inhibition ◽  
Glucose Agar ◽  
Enzyme Ratio

Sucralose (1',4',6' trideoxy-trichloro-galactosucrose) is a nontoxic, intensely sweet sucrose derivative that has been shown to be non-cariogenic in experimental animals. The purpose of this study was to determine whether certain oral bacteria could utilize sucralose. Sucralose, as a sole carbon source, was unable to support growth of ten strains of oral bacteria and dental plaque. When sucralose was incorporated into a liquid medium containing glucose or sucrose, all organisms tested displayed similar pH falls, compared with controls. The incorporation of 126 mmol/L sucralose into glucose agar medium caused total inhibition of growth of Streptococcus sobrinus 6715-17, Streptococcus sanguis 10904, Streptococcus sanguis Challis, Streptococcus salivarius, and Actinomyces viscosus WVU627. Sucralose had no effect on IPS production. Sucralose was not bound to, nor taken up by, cells. Sucralose inhibited the formation of glucan and fructan polymers in proportion to the sucralose-to-enzyme ratio, but independent of the sucrose concentration in the assay mixture. No radioactive polymer was formed from 14C-U-sucralose added to mixtures containing glucosyltransferase (GTF) or fructosyltransferase (FTF). Inhibition of GTF and FTF by sucralose was removed following dialysis of the enzyme/sucralose mixture. These results show that sucralose was not utilized by the oral bacteria tested and that the inhibitory effect of sucralose on GTF and FTF was non-competitive and reversible. The results further support the concept that sucralose is non-cariogenic.

Benzoic and salicylic acids isolated from a glycoside of aspen bark and their effect on Hypoxylon pruinatum

1969 ◽  
Vol 47 (8) ◽  
pp. 1295-1301 ◽  
Author(s):  
Martin Hubbes
Keyword(s):  
Salicylic Acid ◽  
Benzoic Acid ◽  
Thin Layer ◽  
Agar Medium ◽  
Synthetic Medium ◽  
Malt Agar ◽  
Inhibition Of Growth ◽  
Total Inhibition ◽  
Layer Chromatography ◽  
Salicylic Acids

Benzoic and salicylic acids, isolated from an unknown glycoside of aspen bark, were identified as the main fungistatic factors of this compound against Hypoxylon pruinatum (Klotzsche) Cke. The identity of the carboxylic acids was established by thin-layer chromatography and ultraviolet and infrared spectrophotometry.On a malt agar medium, benzoic acid inhibited the growth of the fungus at 1 × 10−3 M, whereas salicylic acid at the same concentration stimulated growth. Complete inhibition of growth of the fungus was obtained with benzoic acid at a concentration of 4 × 10−3 M and with salicylic acid at 5 × 10−3 M. Total inhibition was also obtained when both benzoic and salicylic acids, each at a concentration of 2 × 10−3 M, were simultaneously present in the malt agar medium.On a synthetic medium, benzoic acid and glucose, each at a concentration of 1 × 10−3 M, inhibited the growth of the fungus. At this concentration salicylic acid had no effect.When ammonium nitrate was replaced simultaneously by asparagine, alanine, and glutamine, benzoic acid at 2 × 10−3 M, and glucose at 3 × 10−3 M promoted the growth of the fungus. The same growth was also obtained when benzoic acid at 1 × 10−3 M and glucose at 1 × 10−3 M were both added to the medium.

scholarly journals Interaction of porcine mycoplasmas with fresh animal serum

1971 ◽  
Vol 69 (3) ◽  
pp. 361-368 ◽  
Author(s):  
D. H. Roberts
Keyword(s):  
Synergistic Effect ◽  
Animal Species ◽  
Agar Medium ◽  
Horse Serum ◽  
Rabbit Antiserum ◽  
Inhibition Of Growth ◽  
Heat Labile ◽  
Fresh Serum

SUMMARYWhen fresh animal serum was dropped onto seeded mycoplasma agar plates, inhibition of growth frequently occurred. This effect was dependent on the mycoplasma serotype and on the animal species from which the fresh serum came. This activity of fresh animal serum was heat-labile and would not diffuse through the agar medium. Growth of all the porcine mycoplasma serotypes was inhibited by fresh sheep serum.M. hyorhinis, M. hyopneumoniae, B 3 and the P 45 strains were insensitive to fresh horse serum. The addition of fresh horse serum to specificM. hyorhinisrabbit antiserum-impregnated disks appeared to have a synergistic effect and the combination ofM. hyorhinisantiserum-impregnated disk and fresh horse serum always inhibited the growth ofM. hyorhinisstrains.

scholarly journals EFFECT OF THE HEXANIC EXTRACT OF TAGETES LUCIDA ON THE INHIBITION OF THE GROWTH OF ENTEROBACTERIA SHIGELLA FLEXNERI AND SALMONELLA TYPHI

2020 ◽  
pp. 113-116
Author(s):  
Jorge Ángel Almeida-Villegas ◽  
Rodolfo García-Contreras ◽  
Miriam Deyanira Rodríguez ◽  
Yahira Katherine Porras-Hernández ◽  
Meliksetyan Lilit Surenovna ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Shigella Flexneri ◽  
Salmonella Typhi ◽  
Hexane Extract ◽  
Gram Negative Bacteria ◽  
Active Metabolites ◽  
Inhibition Of Growth ◽  
Total Inhibition ◽  
Medicinal Plant Extracts ◽  
New Strategies

Antibiotic resistance increases the search for new strategies to combat the diseases they cause, and the use of medicinal plants represents a highly effective and valuable strategy, such as the use of Tagetes lucida with different gram positive and gram negative bacteria. Objective: To evaluate the biological activity of the hexane extract of the Tagetes lucida plant at different concentrations on the inhibition of growth in plaque and tube of two enterobacteriaceae, Shigella flexneri and Salmonella typhi Methods: In the following work, a hexane extract from Tagetes lucida was evaluated on the growth inhibition of two enterobacteriaceae, Shigella flexneri and Salmonella typhi using different concentrations of vehicle to evaluate if it affected bacterial growth and also different concentrations of extract to evaluate activity. Results: Once the studies were carried out in triplicate, it was possible to specify that from 75µl/µg of extract, almost total inhibition of the growth of both bacteria was achieved, both in the plate method and in the tube method. And from 100 µl/µg total inhibition is achieved. Conclusions: The favorable results obtained with 75 µl/ µg, confirm that medicinal plant extracts are an important strategy to combat multi-drug resistant bacterial infections. On the other hand, it allows a study to be carried out to evaluate the most active metabolites of the extract, as well as the mechanism of action on the inhibition of the growth of the bacteria under study.

scholarly journals Efeito antimicrobiano in vitro do extrato de jabuticaba [Myrciaria cauliflora (Mart.)O.Berg.] sobre Streptococcus da cavidade oral

2009 ◽  
Vol 11 (1) ◽  
pp. 79-83 ◽  
Author(s):  
C.M. Carvalho ◽  
M.R. Macedo-Costa ◽  
M.S.V. Pereira ◽  
J.S. Higino ◽  
L.F.P.C. Carvalho ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Streptococcus Sobrinus ◽  
Streptococcus Sanguis

Este estudo avaliou a ação do extrato etanólico das folhas de Myrciaria cauliflora (Mart.)O.Berg. sobre três culturas de Streptococcus formadoras do biofilme dental. A atividade antimicrobiana foi determinada pelo método de difusão em meio sólido. Os dados obtidos foram satisfatórios para todas as espécies ensaiadas (Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 27609 e Streptococcus sanguis ATCC 10557) gerando halos de inibição quando utilizado o extrato puro e diluições 1:2 e 1:4. Os resultados incentivam a realização de novas pesquisas que viabilizem a produção de compostos com finalidade terapêutica que possam ser utilizados clinicamente na odontologia.

Influence of Resin Monomers on Growth of Oral Streptococci

2004 ◽  
Vol 83 (4) ◽  
pp. 302-306 ◽  
Author(s):  
Y. Takahashi ◽  
S. Imazato ◽  
R.R.B. Russell ◽  
Y. Noiri ◽  
S. Ebisu
Keyword(s):  
Morphological Observation ◽  
Bacterial Suspension ◽  
Bacterial Cells ◽  
Oral Streptococci ◽  
Streptococcus Sobrinus ◽  
Associated Bacteria ◽  
Streptococcus Sanguis ◽  
Colony Forming Units ◽  
Biomass Increase

Ethyleneglycol dimethacrylate monomers have been previously reported to stimulate the growth of certain caries-associated bacteria on the basis of turbidity measurements. To elucidate the detail of this effect, we examined the influence of resin monomers on the growth of Streptococcus sobrinus and Streptococcus sanguis by determination of bacterial numbers (colony-forming units), morphological observation, and chemical analysis. Although the absorbance values in the stationary phase of bacterial suspension were increased in the presence of ethyleneglycol monomers, no significant differences were observed for bacterial numbers throughout the incubation period. Scanning electron microscopy observation revealed the formation of sparse vesicular material surrounding bacterial cells when incubated with ethyleneglycol monomers, and these products were proved to be resin polymers. The results demonstrate that the apparent biomass increase during incubation with ethyleneglycol monomers is due not to promotion of bacterial multiplication, but to the polymerization of resin monomers to form vesicular structures attached to cells.

Hormone control in regeneration: effects of somatostatin on appendage regeneration, blood glucose and liver glycogen in Diemictylus viridescens

Development ◽  
1977 ◽  
Vol 40 (1) ◽  
pp. 115-124
Author(s):  
S. Vethamany-Globus ◽  
M. Globus ◽  
J. A. Hartford ◽  
I. Fraser ◽  
D. Weber
Keyword(s):  
Blood Glucose ◽  
Liver Glycogen ◽  
Inhibitory Effect ◽  
Thyroid Stimulating Hormone ◽  
Present Communication ◽  
Tail Regeneration ◽  
Experimental Animals ◽  
Total Inhibition ◽  
Daily Dose ◽  
Hormone Control

In the present communication, synthetic somatostatin, a hypothalamic factor which has a known inhibitory effect on the release of growth hormone, thyroid-stimulating hormone, prolactin, insulin and glucagon in man and other mammals, was found to have an inhibitory effect on limb and tail regeneration in adult Diemictylus viridescens, when the newts were treated with a daily dose of 3·5 or 15 µg/animal for a period of 34 days post-amputation. At the higher dose, the animals exhibited total inhibition of appendage regeneration in a few cases and the remainder showed a considerable delay compared to the controls; none of the experimental animals reached the advanced four-digit stage achieved by the controls. Furthermore, the blood glucose and liver glycogen values in the somatostatin-treated animals were significantly lower than the control values. Mechanisms in the storage, mobilization and utilization of glucose (involving hormones) are discussed in relation to appendage regeneration in the newt and possible controls of regeneration at the level of the hypothalamus are suggested.

Inhibitory effect of methyl gallate and gallic acid on oral bacteria

2008 ◽  
Vol 46 (6) ◽  
pp. 744-750 ◽  
Author(s):  
Mi-Sun Kang ◽  
Jong-Suk Oh ◽  
In-Chol Kang ◽  
Suk-Jin Hong ◽  
Choong-Ho Choi
Keyword(s):  
Gallic Acid ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Methyl Gallate

scholarly journals Antimicrobial and Substantivity Properties of Silver Nanoparticles against Oral Microbiomes Clinically Isolated from Young and Young-Adult Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
León Francisco Espinosa-Cristóbal ◽  
Carolina Holguín-Meráz ◽  
Erasto Armando Zaragoza-Contreras ◽  
Rita Elizabeth Martínez-Martínez ◽  
Alejandro Donohue-Cornejo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Young Adult ◽  
Dental Caries ◽  
Periodontal Disease ◽  
Dental Plaque ◽  
Bacterial Species ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Oral Microbiome ◽  
Oral Biofilms

The dental plaque is an oral microbiome hardly associated to be the etiological agent of dental caries and periodontal disease which are still considered serious health public problems. Silver nanoparticles (AgNPs) have demonstrated to have good antimicrobial properties affecting a wide variety of microorganisms, including oral bacteria; however, there is no scientific information that has evaluated the antimicrobial effect of AgNPs against clinical oral biofilms associated with dental caries and periodontal disease. The aim of this study was to determine the antimicrobial and substantivity effects of AgNPs in oral biofilms isolated clinically from patients with dental caries and periodontal disease. Sixty-seven young and young-adult subjects with dental caries and periodontal disease were clinically sampled through the collection of subgingival dental plaque. The inhibitory effect of AgNPs was performed with standard microbiological assays by triplicate using two sizes of particle. Polymerase chain reaction (PCR) assay was used to identify the presence of specific bacterial species. All AgNPs showed an inhibitory effect for all oral biofilms for any age and, generally, any gender (p>0.05); however, the effectiveness of the antimicrobial and substantivity effects was related to particle size, time, and gender (p<0.05). The identified microorganisms were S. mutans, S. sobrinus, S. sanguinis, S. gordonii, S. oralis, P. gingivalis, T. forsythia, and P. intermedia. The AgNPs could be considered as a potential antimicrobial agent for the control and prevention of dental caries and periodontal disease.

Sign in / Sign up

Export Citation Format

Share Document