Cinnamaldehyde’s Potential Inhibitory Effect towards Planktonic and Biofilm of Oral Bacteria

2020 ◽  
Vol 13 (01) ◽  
Keyword(s):  
Inhibitory Effect ◽  
Oral Bacteria

Inhibitory effect of methyl gallate and gallic acid on oral bacteria

2008 ◽  
Vol 46 (6) ◽  
pp. 744-750 ◽  
Author(s):  
Mi-Sun Kang ◽  
Jong-Suk Oh ◽  
In-Chol Kang ◽  
Suk-Jin Hong ◽  
Choong-Ho Choi
Keyword(s):  
Gallic Acid ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Methyl Gallate

scholarly journals Antimicrobial and Substantivity Properties of Silver Nanoparticles against Oral Microbiomes Clinically Isolated from Young and Young-Adult Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
León Francisco Espinosa-Cristóbal ◽  
Carolina Holguín-Meráz ◽  
Erasto Armando Zaragoza-Contreras ◽  
Rita Elizabeth Martínez-Martínez ◽  
Alejandro Donohue-Cornejo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Young Adult ◽  
Dental Caries ◽  
Periodontal Disease ◽  
Dental Plaque ◽  
Bacterial Species ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Oral Microbiome ◽  
Oral Biofilms

The dental plaque is an oral microbiome hardly associated to be the etiological agent of dental caries and periodontal disease which are still considered serious health public problems. Silver nanoparticles (AgNPs) have demonstrated to have good antimicrobial properties affecting a wide variety of microorganisms, including oral bacteria; however, there is no scientific information that has evaluated the antimicrobial effect of AgNPs against clinical oral biofilms associated with dental caries and periodontal disease. The aim of this study was to determine the antimicrobial and substantivity effects of AgNPs in oral biofilms isolated clinically from patients with dental caries and periodontal disease. Sixty-seven young and young-adult subjects with dental caries and periodontal disease were clinically sampled through the collection of subgingival dental plaque. The inhibitory effect of AgNPs was performed with standard microbiological assays by triplicate using two sizes of particle. Polymerase chain reaction (PCR) assay was used to identify the presence of specific bacterial species. All AgNPs showed an inhibitory effect for all oral biofilms for any age and, generally, any gender (p>0.05); however, the effectiveness of the antimicrobial and substantivity effects was related to particle size, time, and gender (p<0.05). The identified microorganisms were S. mutans, S. sobrinus, S. sanguinis, S. gordonii, S. oralis, P. gingivalis, T. forsythia, and P. intermedia. The AgNPs could be considered as a potential antimicrobial agent for the control and prevention of dental caries and periodontal disease.

Inhibitory Effect of a High-Molecular-Weight Constituent of Cranberry on Adhesion of Oral Bacteria

2002 ◽  
Vol 42 (sup3) ◽  
pp. 285-292 ◽  
Author(s):  
E. I. Weiss ◽  
R. Lev-Dor ◽  
Nathan Sharon ◽  
Itzhak Ofek
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Inhibitory Effect ◽  
Oral Bacteria

scholarly journals Microbiological Evaluation of Octenidine Dihydrochloride Mouth Rinse after 5 Days' Use in Orthodontic Patients

2009 ◽  
Vol 79 (4) ◽  
pp. 766-772 ◽  
Author(s):  
Alev Aksoy Dogan ◽  
Emel Sesli Cetin ◽  
Emad Hüssein ◽  
Ali Kudret Adiloglu
Keyword(s):  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Lactobacillus Species ◽  
Orthodontic Appliances ◽  
Cariogenic Bacteria ◽  
Fixed Orthodontic Appliances ◽  
Octenidine Dihydrochloride ◽  
Orthodontic Patients ◽  
Fixed Appliances

Abstract Objective: To determine the absolute and relative antibacterial activity of octenidine dihydrochloride (OCT) against total and cariogenic bacteria in saliva samples of patients with fixed orthodontic appliances during 5 days of usage. Materials and Methods: The study group consisted of 5 male and 13 female subjects who were selected from patients in the Clinic of Orthodontics. Each patient was given physiologic saline (PS), chlorhexidine gluconate (CHX), polyvinylpyrrolidone-iodine complex (PVP-I), and OCT every morning for 5 days, each separated by a 2-week interval. Total and cariogenic bacteria in saliva samples of orthodontically treated patients with fixed appliances were collected during 5 days of usage. Unstimulated saliva was collected as a baseline sample. Saliva samples were collected at 15 minutes, and on the second, third, and fifth day after rinsing the mouth with any of the solutions for 30 seconds, and bacterial counts were detected. Results: OCT showed an ultimate reduction of total viable oral bacteria, Lactobacillus species, and Streptococcus mutans in vivo. OCT also had a significantly greater inhibitory effect than 0.2% CHX and 7.5% PVP-I, from the beginning of the study until the fifth day after the orthodontic appliances were bonded (P &lt; .1). Conclusions: OCT compared favorably with respect to CHX and PVP-I complex in orthodontically treated patients with fixed appliances (P ≤ .1).

The Influence of Sucralose on Bacterial Metabolism

1990 ◽  
Vol 69 (8) ◽  
pp. 1480-1484 ◽  
Author(s):  
D.A. Young ◽  
W.H. Bowen
Keyword(s):  
Agar Medium ◽  
Sucrose Concentration ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Streptococcus Sobrinus ◽  
Streptococcus Sanguis ◽  
Inhibition Of Growth ◽  
Total Inhibition ◽  
Glucose Agar ◽  
Enzyme Ratio

Sucralose (1',4',6' trideoxy-trichloro-galactosucrose) is a nontoxic, intensely sweet sucrose derivative that has been shown to be non-cariogenic in experimental animals. The purpose of this study was to determine whether certain oral bacteria could utilize sucralose. Sucralose, as a sole carbon source, was unable to support growth of ten strains of oral bacteria and dental plaque. When sucralose was incorporated into a liquid medium containing glucose or sucrose, all organisms tested displayed similar pH falls, compared with controls. The incorporation of 126 mmol/L sucralose into glucose agar medium caused total inhibition of growth of Streptococcus sobrinus 6715-17, Streptococcus sanguis 10904, Streptococcus sanguis Challis, Streptococcus salivarius, and Actinomyces viscosus WVU627. Sucralose had no effect on IPS production. Sucralose was not bound to, nor taken up by, cells. Sucralose inhibited the formation of glucan and fructan polymers in proportion to the sucralose-to-enzyme ratio, but independent of the sucrose concentration in the assay mixture. No radioactive polymer was formed from 14C-U-sucralose added to mixtures containing glucosyltransferase (GTF) or fructosyltransferase (FTF). Inhibition of GTF and FTF by sucralose was removed following dialysis of the enzyme/sucralose mixture. These results show that sucralose was not utilized by the oral bacteria tested and that the inhibitory effect of sucralose on GTF and FTF was non-competitive and reversible. The results further support the concept that sucralose is non-cariogenic.

scholarly journals Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis

2017 ◽  
Vol 18 (4) ◽  
pp. 312-316 ◽  
Author(s):  
Aarti A Bohora ◽  
Sharad R Kokate
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Indicator Strain ◽  
Oral Bacteria ◽  
Diffusion Method

ABSTRACT Introduction The main goal of endodontics is the prevention of apical periodontitis. This is due to the presence of persistent pathogenic microorganisms, such as Enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. Lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. This study explores shifting the established paradigm of endodontic treatment, which has focused on eliminating all bacteria from the canal system and on elimination of the problematic bacteria through introducing probiotics. A preliminary work was performed to evaluate the possible effectiveness of probiotics in preventing the growth of E. faecalis. Materials and methods Two probiotic cultures Lactobacillus plantarum ATCC 8041 and Lactobacillus rhamnosus ATCC 7408 were selected to check their antimicrobial activity against E. faecalis ATCC 29212 by two methods: agar cup/well diffusion method and deferred antagonism test. Agar cup method A total of 0.5 mL of requisite test pathogen culture was inoculated into 20 mL of molten sterile Mueller and Hinton agar and cooled to 45 ± 2°C. Circular wells of diameter 10 mm were punched in each of the poured plates. Appropriately diluted test samples were added to the above-punched wells. The plates were incubated upright position at 37°C for 24 hours in aerobic conditions. Postincubation, zone of inhibition was measured. The cell-free supernatant of Lactobacillus species was also evaluated for antimicrobial activity. Deferred antagonism test The test probiotic strain was standardized to 0.1 optical density (OD) at 600 nm and inoculated in a 1 cm wide diametric streak across the surface of trypticase soy agar + yeast extract + calcium carbonate (TSYCa) agar using a sterile cotton swab. Then, the plate agar containing the test strain was incubated at 37°C for 24 hours under aerophilic condition and then standardized to 0.1 OD at 600 nm for overnight (18 hours, 37°C). A purified culture of indicator strain (pathogen) was streaked at right angles to the line of original producer growth. Postincubation plates were observed for the inhibition zone width of the indicator strain. Results Under the conditions of this study, Lactobacilli had an inhibitory effect on the growth of E. faecalis by agar cup method but not by deferred antagonism test. Conclusion This pilot study demonstrated that probiotics show a potential in root canal therapy. Clinical Significance If probioitcs are effective against endodontic pathogens, they can be potentially used as intracanal medicaments. This will be a novel concept of introducing bacteriotherapy in endodontics and replacing pathogenic bacteria by healthy bacteria, normal flora. How to cite this article Bohora AA, Kokate SR. Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis. J Contemp Dent Pract 2017;18(4):312-316.

Inhibitory effect of garlic extract on oral bacteria

2005 ◽  
Vol 50 (7) ◽  
pp. 645-651 ◽  
Author(s):  
I.M. Bakri ◽  
C.W.I. Douglas
Keyword(s):  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Garlic Extract

scholarly journals Inhibitory effect of toothbrush monofilament containing surface pre-reacted glass-ionomer (S-PRG) filler on Streptococcus mutans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saaya Matayoshi ◽  
Ryota Nomura ◽  
Takahiro Kitamura ◽  
Rena Okawa ◽  
Kazuhiko Nakano
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Dental Materials ◽  
Self Care ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Glass Ionomer ◽  
Inhibitory Effects ◽  
Oral Environment

AbstractThe oral environment affects not only oral health, but also general health, and the importance of oral self-care has recently been recognised. Although toothbrushes are the most important self-care product, there are few toothbrushes that have an inhibitory effect on oral bacteria. In the present study, monofilaments used for toothbrushes containing surface pre-reacted glass-ionomer (S-PRG) filler (a component recently applied to various dental materials) were developed. Among nylon and polyester monofilaments commonly used for toothbrushes, nylon monofilaments can accommodate more S-PRG filler than polyester monofilaments, resulting in greater release of ions from the S-PRG filler. These monofilaments containing S-PRG filler formed less biofilm containing Streptococcus mutans, a major pathogen of dental caries, than monofilaments without S-PRG filler. Moreover, S. mutans adhering to monofilaments containing S-PRG filler were more easily exfoliated and eliminated than those adhering to monofilaments without S-PRG filler. Such inhibitory effects on S. mutans were more marked in nylon monofilaments than in polyester monofilaments. These findings that monofilaments containing S-PRG filler can release ions and have an inhibitory effect on S. mutans suggest that they may be an effective material for toothbrushes.

scholarly journals Inhibitory Effect of EUTHYMOL Original Toothpaste on VSC-producing Oral Bacteria

2021 ◽  
Vol 17 (2) ◽  
pp. 75-75
Author(s):  
Hyojin Kim ◽  
Wonkyung Choi ◽  
Wonkyung Lee ◽  
Wonchan Kim ◽  
Kyounghee Oh ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Oral Bacteria

scholarly journals Antimicrobial Activity of Silver Nanoparticles against Clinical Biofilms from Patients with and without Dental Caries

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Alondra Jaqueline Jiménez-Ramírez ◽  
Rita Elizabeth Martínez-Martínez ◽  
José Luis Ayala-Herrera ◽  
Erasto Armando Zaragoza-Contreras ◽  
Rubén Abraham Domínguez-Pérez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Dental Caries ◽  
Dental Plaque ◽  
Scientific Information ◽  
Antimicrobial Properties ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Oral Disease ◽  
Microbiological Analysis

Dental caries is an infectious oral disease originated by the presence of different microorganisms from well-defined biofilms. Many treatments for dental caries have been demonstrated to be successful protocols; however, incidence and prevalence remain still high. Although silver nanoparticles (AgNPs) have shown excellent antimicrobial properties, even against different oral bacteria, there is no available scientific information that has evaluated the antimicrobial activity of AgNPs against oral biofilms from subjects with active dental caries. The objective of this research was to evaluate the inhibitory effect of AgNPs in dental biofilms from subjects with and without dental caries. Two sizes of AgNPs were prepared and characterized. Dental plaque samples were collected from 30 subjects with dental caries and 30 subjects with no dental caries. Microbiological analysis was determined by the minimum inhibitory concentration (MIC) of nanoparticles, and the presence and distribution of microbial strains were identified by polymerase chain reaction (PCR) assay. AgNPs had significant antimicrobial effects against all samples of dental plaque; however, the physical properties of AgNPs, as well as specific sociodemographic and clinical conditions from patients, were also associated with bacterial growth inhibition of Ag particles ( p < 0.05 ). PCR confirmed the presence of oral bacteria associated with dental caries, such as S. mutans and S. sobrinus strains, principally. The AgNPs exhibited great potential to be used as an antimicrobial therapy for the control and prevention of dental caries.

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