A comparative study about the immunomodulatory effects of tramadol and metamizole in a murine model of postoperative ileus

2019 ◽  
Vol 53 (6) ◽  
pp. 610-618 ◽  
Author(s):  
Gun-Soo Hong ◽  
Kathy Stein ◽  
Mariola Lysson ◽  
Joerg Kalff ◽  
Sven Wehner
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Postoperative Ileus ◽  
Gastrointestinal Transit ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Immunomodulatory Effects ◽  
Muscularis Externa ◽  
Underlying Inflammation ◽  
Pro Inflammatory Cytokines

Postoperative ileus (POI) is a common complication after abdominal surgery characterized by motility disturbances leading to increased morbidity and mortality in surgical patients. Intestinal manipulation of the murine small bowel is an established animal model resulting in an increased postsurgical inflammation within the intestinal muscular externa and a delayed gastrointestinal transit. Some analgesics have been shown to affect inflammation. In this study, we compared the immunomodulatory effects of two different analgesics. Mice were treated with tramadol, metamizole or saline as a control in our established POI model. The postoperative inflammatory response was assessed by gene expression of pro-inflammatory cytokines at different time points and immunocytes extravasation into the muscularis externa. Functional motility analyses were performed by a gastrointestinal transit measurement. Metamizole application reduced the pro-inflammatory response after surgery and improved gastrointestinal motility, while tramadol showed no alteration in cytokine gene expression, influx of immunocytes and gastrointestinal transit compared with the controls. In conclusion. we suggest tramadol as analgesia in immunological studies on POI in mice as it does not affect the underlying inflammation of POI.

A role for β 2 integrins (CD11/CD18) in the regulation of cytokine gene expression of polymorphonuclear neutrophils during the inflammatory response

1999 ◽  
Vol 13 (13) ◽  
pp. 1855-1865 ◽  
Author(s):  
Barbara Walzog ◽  
Pamela Weinmann ◽  
Frank Jeblonski ◽  
Karin Scharffetter‐Kochanek ◽  
Kurt Bommert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Polymorphonuclear Neutrophils ◽  
Cytokine Gene Expression ◽  
Cytokine Gene

scholarly journals Cytokine gene expression and molecular detection of Mycobacterium avium subspecies paratuberculosisin organs of experimentally infected mice

2015 ◽  
Vol 35 (5) ◽  
pp. 396-402 ◽  
Author(s):  
David G.G. Schwarz ◽  
Pricila A.G. Pietralonga ◽  
Marina C.C. Souza ◽  
Isabel A. Carvalho ◽  
Rosyane S. Cruzeiro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Molecular Detection ◽  
Experimental Models ◽  
Probability Of Detection ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Direct Role ◽  
Tnf Α ◽  
Ifn Γ

AbstractMycobacterium avium subspecies paratuberculosis (MAP) can infect ruminants and remain subclinical for long periods within herds. The identification of organs that are more susceptible to infection and the evaluation of cytokine expression at the site of infection are important to understand the pathogenesis of MAP. In this study, the probability of detection of MAP-DNA and the expression of cytokines in organs of C57BL/6 mice infected intraperitoneally for 120 days were evaluated. Among the evaluated organs, the spleen (85%), colon (75%) and liver (60%) had the highest frequency of positivity. When compared these frequencies between organs, it has been found that the spleen had 1.54 times as likely to be positive in relation to the ileum, and 2.0 times more likely in relation to the Peyer's patches. In addition, at 60 days post-infection, the spleen and the liver were responsible for upregulation of IFN-γ , and the ileum by TNF-α and IL-4. The results indicate that the spleen is the best organ for evaluating an experimental infection by MAP, especially in the initial stages of the infection. Moreover, it showed that the spleen, liver and ileum have a direct role in the inflammatory response in experimental models.

Immunomodulatory Effects of Monascus spp.-Fermented Sacccharina japonica Extracts on the Cytokine Gene Expression of THP-1 Cells

2018 ◽  
Vol 188 (2) ◽  
pp. 498-513 ◽  
Author(s):  
Sharmin Suraiya ◽  
Won Je Jang ◽  
Hwa Jin Cho ◽  
Yu Bin Choi ◽  
Hae Dae Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Immunomodulatory Effects

scholarly journals Cytokine gene regulation by PGE2, LTB4and PAF

1992 ◽  
Vol 1 (1) ◽  
pp. 5-8 ◽  
Author(s):  
M. Rola-Pleszczynski ◽  
J. Stankova
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Gene Regulation ◽  
Inflammatory Response ◽  
Cytokine Production ◽  
Initial Response ◽  
Lipid Mediators ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Regulatory Effects

The initial response of the host to noxious stimuli produces a nonspecific inflammatory response. A more specific immune response is believed to be modulated by two classes of molecules: lipid mediators (PG, LT and PAF) and cytokines, synthesized by phagocytes and parenchyreal cells. In this review we discuss the increasing evidence of the interrelationship between eicosanoids, PAF and cytokines: IL-1 and TNF induce PG synthesis in various cells and PG, in turn, modulate cytokine production. We focused on the regulatory effects ofLTB4,PGE2and PAF on cytokine gene expression.

scholarly journals Cytokine gene expression in skin of susceptible guinea‐pig infected withTreponema pallidum

Immunology ◽  
1998 ◽  
Vol 95 (2) ◽  
pp. 242-247 ◽  
Author(s):  
WICHER ◽  
SCAROZZA ◽  
RAMSINGH ◽  
WICHER
Keyword(s):  
Gene Expression ◽  
Guinea Pig ◽  
Cytokine Gene Expression ◽  
Cytokine Gene

scholarly journals Epigenetic Regulation of Cytokine Gene Expression in T Lymphocytes

2009 ◽  
Vol 50 (3) ◽  
pp. 322 ◽  
Author(s):  
Choong-Gu Lee ◽  
Anupama Sahoo ◽  
Sin-Hyeog Im
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Epigenetic Regulation ◽  
Cytokine Gene Expression ◽  
Cytokine Gene
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