scholarly journals Cytokine gene regulation by PGE2, LTB4and PAF

1992 ◽  
Vol 1 (1) ◽  
pp. 5-8 ◽  
Author(s):  
M. Rola-Pleszczynski ◽  
J. Stankova
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Gene Regulation ◽  
Inflammatory Response ◽  
Cytokine Production ◽  
Initial Response ◽  
Lipid Mediators ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Regulatory Effects

The initial response of the host to noxious stimuli produces a nonspecific inflammatory response. A more specific immune response is believed to be modulated by two classes of molecules: lipid mediators (PG, LT and PAF) and cytokines, synthesized by phagocytes and parenchyreal cells. In this review we discuss the increasing evidence of the interrelationship between eicosanoids, PAF and cytokines: IL-1 and TNF induce PG synthesis in various cells and PG, in turn, modulate cytokine production. We focused on the regulatory effects ofLTB4,PGE2and PAF on cytokine gene expression.

A comparative study about the immunomodulatory effects of tramadol and metamizole in a murine model of postoperative ileus

2019 ◽  
Vol 53 (6) ◽  
pp. 610-618 ◽  
Author(s):  
Gun-Soo Hong ◽  
Kathy Stein ◽  
Mariola Lysson ◽  
Joerg Kalff ◽  
Sven Wehner
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Postoperative Ileus ◽  
Gastrointestinal Transit ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Immunomodulatory Effects ◽  
Muscularis Externa ◽  
Underlying Inflammation ◽  
Pro Inflammatory Cytokines

Postoperative ileus (POI) is a common complication after abdominal surgery characterized by motility disturbances leading to increased morbidity and mortality in surgical patients. Intestinal manipulation of the murine small bowel is an established animal model resulting in an increased postsurgical inflammation within the intestinal muscular externa and a delayed gastrointestinal transit. Some analgesics have been shown to affect inflammation. In this study, we compared the immunomodulatory effects of two different analgesics. Mice were treated with tramadol, metamizole or saline as a control in our established POI model. The postoperative inflammatory response was assessed by gene expression of pro-inflammatory cytokines at different time points and immunocytes extravasation into the muscularis externa. Functional motility analyses were performed by a gastrointestinal transit measurement. Metamizole application reduced the pro-inflammatory response after surgery and improved gastrointestinal motility, while tramadol showed no alteration in cytokine gene expression, influx of immunocytes and gastrointestinal transit compared with the controls. In conclusion. we suggest tramadol as analgesia in immunological studies on POI in mice as it does not affect the underlying inflammation of POI.

A role for β 2 integrins (CD11/CD18) in the regulation of cytokine gene expression of polymorphonuclear neutrophils during the inflammatory response

1999 ◽  
Vol 13 (13) ◽  
pp. 1855-1865 ◽  
Author(s):  
Barbara Walzog ◽  
Pamela Weinmann ◽  
Frank Jeblonski ◽  
Karin Scharffetter‐Kochanek ◽  
Kurt Bommert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Polymorphonuclear Neutrophils ◽  
Cytokine Gene Expression ◽  
Cytokine Gene

scholarly journals Cytokine gene expression and molecular detection of Mycobacterium avium subspecies paratuberculosisin organs of experimentally infected mice

2015 ◽  
Vol 35 (5) ◽  
pp. 396-402 ◽  
Author(s):  
David G.G. Schwarz ◽  
Pricila A.G. Pietralonga ◽  
Marina C.C. Souza ◽  
Isabel A. Carvalho ◽  
Rosyane S. Cruzeiro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Molecular Detection ◽  
Experimental Models ◽  
Probability Of Detection ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Direct Role ◽  
Tnf Α ◽  
Ifn Γ

AbstractMycobacterium avium subspecies paratuberculosis (MAP) can infect ruminants and remain subclinical for long periods within herds. The identification of organs that are more susceptible to infection and the evaluation of cytokine expression at the site of infection are important to understand the pathogenesis of MAP. In this study, the probability of detection of MAP-DNA and the expression of cytokines in organs of C57BL/6 mice infected intraperitoneally for 120 days were evaluated. Among the evaluated organs, the spleen (85%), colon (75%) and liver (60%) had the highest frequency of positivity. When compared these frequencies between organs, it has been found that the spleen had 1.54 times as likely to be positive in relation to the ileum, and 2.0 times more likely in relation to the Peyer's patches. In addition, at 60 days post-infection, the spleen and the liver were responsible for upregulation of IFN-γ , and the ileum by TNF-α and IL-4. The results indicate that the spleen is the best organ for evaluating an experimental infection by MAP, especially in the initial stages of the infection. Moreover, it showed that the spleen, liver and ileum have a direct role in the inflammatory response in experimental models.

scholarly journals Local and systemic immune response in patients withsevere atopic dermatitis

2011 ◽  
Vol 8 (5) ◽  
pp. 10-15
Author(s):  
T M Filimonova ◽  
Ol'ga Gur'evna Elisyutina ◽  
E S Fedenko ◽  
D D Niyazov ◽  
M N Boldyreva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Atopic Dermatitis ◽  
Peripheral Blood ◽  
Transforming Growth Factor ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Healthy Individuals ◽  
Th2 Response ◽  
Healthy Donors

Background. to comparatively investigate cytokine gene expression in the skin and peripheral blood of atopic dermatitis (AD) patients and healthy individuals. Methods. Samples of skin and peripheral blood from 48 severe AD patients SCORAD (Scoring Atopic Dermatitis) 78,5 [57; 89], IGA (Investigators Global Assessment) 4,2 [3,9; 4,7]) at the age of 17 to 45 years and 20 healthy donors aged from 19 to 32 years were analyzed for gene expression of cytokines using real time reverse transcription polymerase chain reaction (RT-PCR). Results. In the skin of patients with AD, a significant increase of the level of gene expression was observed for interleukin IL2R (interleukin) (р=0,0023), IL5 (р=0,002), IL6 (р=0,0023), IL8 (р=0,01), IL12β (р=0,0023), IL10 (р=0,0023), IL23 (р=0,002), IL29 (р=0,0023), and TGFβ (transforming growth factor) (p=0,0023) as compared to healthy individuals. In contrast, no difference between AD patients and healthy donors was detected with respect to cytokine gene expression in the peripheral blood. Conclusions. Activity of IL-2R, IL-8, IL-12β, IL-23, IL-29, and TGFβ that are markers of chronic inflammation and Th1 immune response in severe AD and IL-5, IL-10 that are anti-inflammatory cytokines and markers of Th2 response was predominant in the skin but not in the blood of AD patients.

scholarly journals Ambient ultrafine particles provide a strong adjuvant effect in the secondary immune response: implication for traffic-related asthma flares

2010 ◽  
Vol 299 (3) ◽  
pp. L374-L383 ◽  
Author(s):  
Ning Li ◽  
Jack R. Harkema ◽  
Ryan P. Lewandowski ◽  
Meiying Wang ◽  
Lori A. Bramble ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Airway Inflammation ◽  
Ultrafine Particles ◽  
Allergic Airway Inflammation ◽  
Cytokine Gene Expression ◽  
Secondary Immune Response ◽  
Cytokine Gene ◽  
Adjuvant Effect ◽  
Traffic Exposure

We have previously demonstrated that intranasal administration of ambient ultrafine particles (UFP) acts as an adjuvant for primary allergic sensitization to ovalbumin (OVA) in Balb/c mice. It is important to find out whether inhaled UFP exert the same effect on the secondary immune response as a way of explaining asthma flares in already-sensitized individuals due to traffic exposure near a freeway. The objective of this study is to determine whether inhalation exposure to ambient UFP near an urban freeway could enhance the secondary immune response to OVA in already-sensitized mice. Prior OVA-sensitized animals were exposed to concentrated ambient UFP at the time of secondary OVA challenge in our mobile animal laboratory in Los Angeles. OVA-specific antibody production, airway morphometry, allergic airway inflammation, cytokine gene expression, and oxidative stress marker were assessed. As few as five ambient UFP exposures were sufficient to promote the OVA recall immune response, including generating allergic airway inflammation in smaller and more distal airways compared with the adjuvant effect of intranasally instilled UFP on the primary immune response. The secondary immune response was characterized by the T helper 2 and IL-17 cytokine gene expression in the lung. In summary, our results demonstrated that inhalation of prooxidative ambient UFP could effectively boost the secondary immune response to an experimental allergen, indicating that vehicular traffic exposure could exacerbate allergic inflammation in already-sensitized subjects.

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