scholarly journals Ovarian and Uterine Grafts

1934 ◽  
Vol 27 (10) ◽  
pp. 1395-1406 ◽  
Author(s):  
Max Cheval

Recent experience has convinced the author of the value of ovarian grafting. He gives the histories of a number of women upon whom he made implants of their own ovarian tissue in the course of operations for double oöphorectomy. The subsequent state of these patients is contrasted with that of women after castration without grafting. When the uterus was left in position menstruation was re-established in over 80% of the grafted cases. The author and his co-workers maintain that the vitality of autogenous grafts of ovarian substance is enhanced by hormones produced by the uterine mucosa. He therefore advocates that grafts of uterus be made in conjunction with ovarian implants whenever possible. Experiments carried out on animals have proved the correctness of this opinion. The results of a series of cases of combined ovarian and uterine graftings are recorded, and the technique of uterine mucosa implants is described.

2014 ◽  
Vol 5 (4) ◽  
pp. 158-163 ◽  
Author(s):  
Bernadette A John

From my recent experience of teaching and assessing, and attending national and international medical education conferences, it has become clear to me that the ease of search and replication, occasional absence of details such as date, copyright notice or an obvious author, coupled with the fact that Google is a search engine and not a repository of free images, is poorly understood by clinical students, clinicians and academics alike. All are arguably aware of the implications of plagiarism and the importance of permission and acknowledgement for the contribution made in research and publications.


1986 ◽  
Vol 94 (3) ◽  
pp. 310-315 ◽  
Author(s):  
Richard Upton ◽  
Thomas V. McCaffrey ◽  
John Ellis

Bilateral nerve cable graft repairs were made in the sciatic nerves of Wistar rats. One group of repairs was treated locally with triamcinolone acetonide (0.5 mg), one group was exposed to the systemic effects of the drug, and one group was left untreated. Nerve regeneration was measured by nerve-stimulated muscle twitch strength, as well as several electrophysiologic parameters of the compound action potential conducted through the graft. Results suggest that nerve regeneration through an autogenous graft is significantly improved by local treatment with triamcinolone acetonide. Further studies are indicated to determine appropriate dosage necessary to maximize the beneficial local effects.


2019 ◽  
Vol 39 (12) ◽  
pp. 997-1004
Author(s):  
Mariana G. Rêgo ◽  
Maria Lucia G. Araujo ◽  
Maria Edna G. Barros ◽  
Lorena D’Andrade Aires ◽  
Paulo G.V. Oliveira ◽  
...  

ABSTRACT: The nurse shark, Ginglymostoma cirratum (Bonnaterre, 1778) is one of the most studied species of elasmobranchs. However, the knowledge of their reproductive biology is still relatively rare, particularly in the western South Atlantic. This study aimed to describe the morphology of the uterus and the ovary of G. cirratum, based on specimens caught off at the Fortaleza/CE coast, northeast Brazil. Samples were collected from September 2012 to June 2013, from regular landings of artisanal fishing, which commercialize this species freely. A total of ten females were collected. The methodologies followed for analyzing the ovaries and uterus of those females included both macroscopic and histological analysis. G. cirratum has internal type ovary morphology, with invaginations of connective tissue, which defines compartments and separate oocyte groups in ovigerous lots. The epithelium lining the ovary changes from simple columnar ciliated in the area without ovigerous lots, which turns into a simple cubic epithelium in the coating portion of the epigonal organ where ovarian tissue is absent. The uterine mucosa has secretory cells denoted by Alcian Blue staining, indicating the production of mucopolysaccharides, even in immature individuals. This lecithotrophic shark has a uterine vascularized mucosa that is one characteristic of viviparous elasmobranch species.


1969 ◽  
Vol 45 (1) ◽  
pp. 91-97 ◽  
Author(s):  
B. T. DONOVAN

SUMMARY Ovarian tissue was autografted to the spleen or kidney of spayed anoestrous or oestrous ferrets to see whether inactivation of ovarian hormones occurred in the liver and to examine the feedback action of gonadal hormones on gonadotrophin secretion. Although the grafts survived in both sites as did homografts made in anoestrous females, the secretion of gonadal hormones was sufficient to cause oestrus only in a minority of animals and there was little difference in the function of grafts made to the spleen or kidney. Vulval swelling and uterine growth were caused by pellets of oestradiol inserted into the spleen so that it appears that this steroid can pass through the liver without loss of oestrogenic activity. It is concluded that little inactivation of gonadal steroids by the liver of the ferret takes place.


2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jennia Michaeli ◽  
Michael Weintraub ◽  
Eitan Gross ◽  
Yehuda Ginosar ◽  
Vardit Ravitsky ◽  
...  

Children that undergo treatment for cancer are at risk of suffering from subfertility or hormonal dysfunction due to the detrimental effects of radiotherapy and chemotherapeutic agents on the gonads. Cryopreservation of ovarian tissue prior to treatment offers the possibility of restoring gonadal function after resumption of therapy. Effective counseling and management of pediatric patients is crucial for preserving their future reproductive potential. The purpose of this article is to review recent literature and to revise recommendations we made in a 2007 article. Pediatric hemato-oncology, reproductive endocrinology, surgery, anesthesia and bioethics perspectives are discussed and integrated to propose guidelines for offering ovarian cryopreservation to premenarcheal girls with cancer.


2007 ◽  
Vol 39 (1) ◽  
pp. 133-166 ◽  
Author(s):  
JAMES DUNKERLEY

This essay, written in September 2006, considers the first months of the MAS government headed by Evo Morales in the light of the virtually constant political crisis in Bolivia since 2000. The first part asks why the turbulent course of public life in Bolivia has proved so difficult to explain. It seeks to show that the recent period has been depicted in rather narrow interpretations that stress institutional failings, poverty and oppression, or civic heroism, but do not try to find the linkages between these phenomena. The second section proposes an alternative approach, treating the recent experience of conflict as a revolutionary episode in which the idea of ‘Two Bolivias’ needs to be qualified by appreciation of past revolutionary experiences. The final sections suggest that the ardour and complexities of the current conflict might seem more comprehensible if the MAS and its supporters are viewed as essentially plebeian in both condition and ideological disposition. Such a classical and early modern allusion provides a fuller analytical palate for understanding the current conjuncture and the socio-political propositions being made in a ‘semi-modern’ environment.


1966 ◽  
Vol 24 ◽  
pp. 118-119
Author(s):  
Th. Schmidt-Kaler

I should like to give you a very condensed progress report on some spectrophotometric measurements of objective-prism spectra made in collaboration with H. Leicher at Bonn. The procedure used is almost completely automatic. The measurements are made with the help of a semi-automatic fully digitized registering microphotometer constructed by Hög-Hamburg. The reductions are carried out with the aid of a number of interconnected programmes written for the computer IBM 7090, beginning with the output of the photometer in the form of punched cards and ending with the printing-out of the final two-dimensional classifications.


Author(s):  
Mai M. Said ◽  
Ramesh K. Nayak ◽  
Randall E. McCoy

Burgos and Wislocki described changes in the mucosa of the guinea pig uterus, cervix and vagina during the estrous cycle investigated by transmission electron microscopy. More recently, Moghissi and Reame reported the effects of progestational agents on the human female reproductive tract. They found drooping and shortening of cilia in norgestrel and norethindrone- treated endometria. To the best of our knowledge, no studies concerning the effects of mestranol and norethindrone given concurrently on the three-dimensional surface features on the uterine mucosa of the guinea pig have been reported. The purpose of this study was to determine the effect of mestranol and norethindrone on surface ultrastructure of guinea pig uterus by SEM.Seventy eight animals were used in this study. They were allocated into two groups. Group 1 (20 animals) was injected intramuscularly 0.1 ml vegetable oil and served as controls.


Author(s):  
J. Temple Black ◽  
William G. Boldosser

Ultramicrotomy produces plastic deformation in the surfaces of microtomed TEM specimens which can not generally be observed unless special preparations are made. In this study, a typical biological composite of tissue (infundibular thoracic attachment) infiltrated in the normal manner with an embedding epoxy resin (Epon 812 in a 60/40 mixture) was microtomed with glass and diamond knives, both with 45 degree body angle. Sectioning was done in Portor Blum Mt-2 and Mt-1 microtomes. Sections were collected on formvar coated grids so that both the top side and the bottom side of the sections could be examined. Sections were then placed in a vacuum evaporator and self-shadowed with carbon. Some were chromium shadowed at a 30 degree angle. The sections were then examined in a Phillips 300 TEM at 60kv.Carbon coating (C) or carbon coating with chrom shadowing (C-Ch) makes in effect, single stage replicas of the surfaces of the sections and thus allows the damage in the surfaces to be observable in the TEM. Figure 1 (see key to figures) shows the bottom side of a diamond knife section, carbon self-shadowed and chrom shadowed perpendicular to the cutting direction. Very fine knife marks and surface damage can be observed.


Author(s):  
M. Ashraf ◽  
F. Thompson ◽  
S. Miki ◽  
P. Srivastava

Iron is believed to play an important role in the pathogenesis of ischemic injury. However, the sources of intracellular iron in myocytes are not yet defined. In this study we have attempted to localize iron at various cellular sites of the cardiac tissue with the ferrocyanide technique.Rat hearts were excised under ether anesthesia. They were fixed with coronary perfusion with 3% buffered glutaraldehyde made in 0.1 M cacodylate buffer pH 7.3. Sections, 60 μm in thickness, were cut on a vibratome and were incubated in the medium containing 500 mg of potassium ferrocyanide in 49.5 ml H2O and 0.5 ml concentrated HC1 for 30 minutes at room temperature. Following rinses in the buffer, tissues were dehydrated in ethanol and embedded in Spurr medium.The examination of thin sections revealed intense staining or reaction product in peroxisomes (Fig. 1).


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