Selection of Chemicals for the Development and Evaluation of In Vitro Methods for Skin Sensitisation Testing

The prevalence of obesity is increasing globally. Despite the availability of a variety of anti-obesogenic drugs, including therapies under clinical development, these treatments are often indicated for patients with severe obesity, making them unsuitable for patients with mild obesity or for preventative use. In Thailand, traditional remedies employing medicinal plants are widely used to maintain health and treat disease. These treatments are generally inexpensive and readily available at markets, making them good treatment options for preventing obesity. To evaluate the anti-obesogenic potential of Thai medicinal plants, we employed three in vitro methods: pancreatic lipase inhibition, lipolysis enhancement, and lipid accumulation reduction assays. Among 70 Thai medicinal plants, Eurycoma longifolia Jack, Tiliacora triandra Diels, and Acacia concinna (Willd.) DC. were selected as the most favorable candidates because they exhibited anti-obesogenic activity in all three assays. These medicinal plants are expected to have efficient anti-obesogenic effects, making them promising candidates for further study.

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Investigation of in vitro methods for screening fungal probiotics

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600020614 ◽

1991 ◽

Vol 1991 ◽

pp. 111-111

Author(s):

C.J. Newbold ◽

P.P. Frumholtz ◽

R.J. Wallace

Keyword(s):

Saccharomyces Cerevisiae ◽

Rapid Method ◽

Probiotic Properties ◽

Growth Promoters ◽

Short Term ◽

Fermentation Products ◽

In Vitro Methods ◽

Selection Of

Fungal probiotics are gaining increasing acceptance as growth promoters that act via the rumen fermentation. Products have been described based on Saccharomyces cerevisiae and Aspergillus oryzac (Williams and Newbold, 1990). However not all species of Aspergillus or strains of S. cerevisiae exhibit probiotic properties (Tapia and llerrera-Saldana, 1989; Newbold, 1990). The selection of fungi with probiotic properties would be greatly facilitated by the development of a rapid method for assessing their efficacy. In the present study the effects of one of these products (Amaferm, based on Aspergillus oryzae; AO) on short term incubations invitro were compared with results obtained previously in longer-term in vitro fermentations (Rusitec) and with in vivo measurements.

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The Overt and Hidden Use of Animal-Derived Products in Alternative Methods for Skin Sensitisation: A Systematic Review

Alternatives to Laboratory Animals ◽

10.1177/0261192919896361 ◽

2019 ◽

Vol 47 (5-6) ◽

pp. 174-195 ◽

Cited By ~ 2

Author(s):

Bianca Marigliani ◽

Felipe Perraro Sehn ◽

Josemar Vinicius Maiworm Abreu Silva ◽

Luciene Bottentuit López Balottin ◽

Elisabeth de Fatima Pires Augusto ◽

...

Keyword(s):

Systematic Review ◽

Safety Assessment ◽

Alternative Methods ◽

Animal Origin ◽

Animal Testing ◽

Complete Replacement ◽

In Vitro Methods ◽

Skin Sensitisation ◽

Animal Alternatives

In vitro methods that can replace animal testing in the identification of skin sensitisers are now a reality. However, as cell culture and related techniques usually rely on animal-derived products, these methods may be failing to address the complete replacement of animals in safety assessment. The objective of this study was to identify the animal-derived products that are used as part of in vitro methods for skin sensitisation testing. Thus, a systematic review of 156 articles featuring 83 different in vitro methods was carried out and, from this review, the use of several animal-derived products from different species was identified, with the use of fetal bovine serum being cited in most of the methods (78%). The use of sera from other animals, monoclonal antibodies and animal proteins were also variously mentioned. While non-animal alternatives are available and methods free of animal-derived products are emerging, most of the current methods reported used at least one animal-derived product, which raises ethical and technical concerns. Therefore, to deliver technically and ethically better in vitro methods for the safety assessment of chemicals, more effort should be made to replace products of animal origin in existing methods and to avoid their use in the development of new method protocols.

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In vitro negative selection of viral superantigen-reactive TCR transgenic thymocytes by thymic dendritic cells

Immunology Letters ◽

10.1016/s0165-2478(97)87831-5 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 249

Author(s):

I Ferrero

Keyword(s):

Dendritic Cells ◽

Negative Selection ◽

Selection Of

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Anti-amnesic activity screening of the seed ethanol extracts of Turkish Paeonia taxa by in vitro methods

Planta Medica ◽

10.1055/s-0031-1282924 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

D Sevim ◽

FS Senol ◽

I Orhan ◽

B Şener ◽

E Kaya

Keyword(s):

In Vitro Methods ◽

Activity Screening ◽

Ethanol Extracts

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Analysis of antidepressant properties of some Papaver species by in vivo and in vitro methods

Planta Medica ◽

10.1055/s-0035-1565761 ◽

2015 ◽

Vol 81 (16) ◽

Cited By ~ 2

Author(s):

OML Bayazeid ◽

F Yalcin ◽

M İlhan ◽

H Karahan ◽

E Kupeli-Akkol ◽

...

Keyword(s):

In Vitro Methods

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Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽

10.1055/s-0035-1565606 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

R Bertóti ◽

Á Alberti ◽

A Böszörményi ◽

R Könye ◽

T Horváth ◽

...

Keyword(s):

Hairy Root ◽

Hairy Root Cultures ◽

Root Cultures ◽

Clone Selection ◽

Selection Of

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The Effects of Some Synthetic Compounds on In Vitro Fibrinolytic Activity Measured by Different Methods and the Relevance to Activity In Vivo

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649018 ◽

1972 ◽

Vol 28 (03) ◽

pp. 351-358

Author(s):

A.J Baillie ◽

A. K Sim

Keyword(s):

Inhibitory Activity ◽

Substrate Concentration ◽

Fibrinolytic Activity ◽

Synthetic Compounds ◽

In Vitro Methods ◽

Narrow Concentration Range

SummaryThe activity of several synthetic compounds, rated from good to poor (or inactive) fibrinolytic activators, has been assessed by two different commonly-used in vitro methods. Compounds shown to be active over a narrow concentration range in the hanging clot test were shown to be inhibitors of plasmin and trypsin in the casein-olytic test. The inhibitory activity of these compounds was shown to increase with increasing substrate concentration and apparent activity in the hanging clot test. Possible explanations and relevance of these observations are discussed.

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