White matter imaging of ethanol-fixed rat brain by phase-contrast X-ray computed tomography

2021 ◽  
pp. 028418512110307
Author(s):  
Thet-Thet- Lwin ◽  
Akio Yoneyama ◽  
Shogo Kokubo ◽  
Hiroko Maruyama ◽  
Kazuyuki Hyodo ◽  
...  
Keyword(s):  
White Matter ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Image Contrast ◽  
Cerebral White Matter ◽  
Tissue Fixation ◽  
X Ray ◽  
Density Values ◽  
X Ray Computed ◽  
Formalin Fixed

Background Phase-contrast X-ray computed tomography imaging (PCI) based on crystal X-ray interferometry can detect minute density differences within biological soft tissues without contrast agents. Ethanol fixation yields increased tissue-background density differences due to the dehydrating and delipidifying effects of ethanol. Purpose To obtain high image contrast of cerebral white matter structures in PCI, tissue fixation using ethanol and routinely used formalin have been examined. Material and Methods Ethanol-fixed (EF) (n = 4) and formalin-fixed (FF) (n = 4) rat brains were imaged by crystal X-ray interferometry-based PCI. Tissue staining/microscopy was also performed for histological comparison and myelin density evaluation. Three-dimensional white matter tract images were reconstructed. Results Superior image contrast was obtained in the images of EF brains (EF images) compared to those of formalin-fixed brains (FF images), particularly for white matter structures. Significant density differences between the white matter structures and hippocampus ( P < 0.01)/thalamus ( P < 0.001) were observed in the EF, but not FF, images. Ethanol fixation enhanced the image contrast of white matter tracts by approximately sixfold compared to formalin fixation, and close agreement (r2 = 0.97; P < 0.05) between the density values on the CT images and the myelin density values in histological images was observed for the EF brains. Three-dimensional reconstruction of the white matter tracts was possible from the EF images, but not FF images. Conclusion Ethanol fixation resulted in marked contrast enhancement of cerebral white matter structures in PCI. Thus, high-resolution PCI using ethanol for tissue fixation could be valuable for experimental neurological studies and postmortem neuropathology evaluation.

scholarly journals A minute fossil phoretic mite recovered by phase-contrast X-ray computed tomography

2011 ◽  
Vol 8 (3) ◽  
pp. 457-460 ◽  
Author(s):  
Jason A. Dunlop ◽  
Stefan Wirth ◽  
David Penney ◽  
Andrew McNeil ◽  
Robert S. Bradley ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Morphological Characters ◽  
Three Dimensional Model ◽  
X Ray ◽  
Extant Species ◽  
Minimum Age ◽  
X Ray Computed ◽  
Body Fossil

High-resolution phase-contrast X-ray computed tomography (CT) reveals the phoretic deutonymph of a fossil astigmatid mite (Acariformes: Astigmata) attached to a spider's carapace (Araneae: Dysderidae) in Eocene (44–49 Myr ago) Baltic amber. Details of appendages and a sucker plate were resolved, and the resulting three-dimensional model demonstrates the potential of tomography to recover morphological characters of systematic significance from even the tiniest amber inclusions without the need for a synchrotron. Astigmatids have an extremely sparse palaeontological record. We confirm one of the few convincing fossils, potentially the oldest record of Histiostomatidae. At 176 µm long, we believe this to be the smallest arthropod in amber to be CT-scanned as a complete body fossil, extending the boundaries for what can be recovered using this technique. We also demonstrate a minimum age for the evolution of phoretic behaviour among their deutonymphs, an ecological trait used by extant species to disperse into favourable environments. The occurrence of the fossil on a spider is noteworthy, as modern histiostomatids tend to favour other arthropods as carriers.

scholarly journals Three‐dimensional X‐ray‐computed tomography of 3300‐ to 6000‐year‐old Citrullus seeds from Libya and Egypt compared to extant seeds throws doubts on species assignments

2021 ◽  
Author(s):  
Katherine A. Wolcott ◽  
Guillaume Chomicki ◽  
Yannick M. Staedler ◽  
Krystyna Wasylikowa ◽  
Mark Nesbitt ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
X Ray ◽  
X Ray Computed

scholarly journals Tractography Alterations in the Arcuate and Uncinate Fasciculi in Post-Stroke Aphasia

2021 ◽  
Vol 11 (1) ◽  
pp. 53
Author(s):  
Sara Kierońska ◽  
Milena Świtońska ◽  
Grzegorz Meder ◽  
Magdalena Piotrowska ◽  
Paweł Sokal
Keyword(s):  
White Matter ◽  
Brain Connectivity ◽  
Three Dimensional ◽  
Cerebral White Matter ◽  
Neural Pathways ◽  
Uncinate Fasciculus ◽  
Arcuate Fasciculus ◽  
Fiber Tractography ◽  
Post Stroke

Fiber tractography based on diffuse tensor imaging (DTI) can reveal three-dimensional white matter connectivity of the human brain. Tractography is a non-invasive method of visualizing cerebral white matter structures in vivo, including neural pathways surrounding the ischemic area. DTI may be useful for elucidating alterations in brain connectivity resulting from neuroplasticity after stroke. We present a case of a male patient who developed significant mixed aphasia following ischemic stroke. The patient had been treated by mechanical thrombectomy followed by an early rehabilitation, in conjunction with transcranial direct current stimulation (tDCS). DTI was used to examine the arcuate fasciculus and uncinate fasciculus upon admission and again at three months post-stroke. Results showed an improvement in the patient’s symptoms of aphasia, which was associated with changes in the volume and numbers of tracts in the uncinate fasciculus and the arcuate fasciculus.

Visualizing Fluid Flows With X-Rays

2007 ◽  
Author(s):  
Theodore J. Heindel ◽  
Terrence C. Jensen ◽  
Joseph N. Gray
Keyword(s):  
Computed Tomography ◽  
Flow Visualization ◽  
Three Dimensional ◽  
Fluid Flows ◽  
X Rays ◽  
Radiographic Images ◽  
Optical Access ◽  
X Ray ◽  
X Ray Computed ◽  
Density Map

There are several methods available to visualize fluid flows when one has optical access. However, when optical access is limited to near the boundaries or not available at all, alternative visualization methods are required. This paper will describe flow visualization using an X-ray system that is capable of digital X-ray radiography, digital X-ray stereography, and digital X-ray computed tomography (CT). The unique X-ray flow visualization facility will be briefly described, and then flow visualization of various systems will be shown. Radiographs provide a two-dimensional density map of a three dimensional process or object. Radiographic images of various multiphase flows will be presented. When two X-ray sources and detectors simultaneously acquire images of the same process or object from different orientations, stereographic imaging can be completed; this type of imaging will be demonstrated by trickling water through packed columns and by absorbing water in a porous medium. Finally, local time-averaged phase distributions can be determined from X-ray computed tomography (CT) imaging, and this will be shown by comparing CT images from two different gas-liquid sparged columns.

scholarly journals Visualization Ability of Phase-Contrast Synchrotron-Based X-Ray Imaging Using an X-Ray Interferometer in Soft Tissue Tumors

2021 ◽  
Vol 20 ◽  
pp. 153303382110101
Author(s):  
Thet-Thet Lwin ◽  
Akio Yoneyama ◽  
Hiroko Maruyama ◽  
Tohoru Takeda
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
Soft Tissue Tumors ◽  
X Ray ◽  
3 Dimensional ◽  
Computed Tomography Images ◽  
X Ray Imaging ◽  
X Ray Computed

Phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer provides high sensitivity and high spatial resolution, and it has the ability to depict the fine morphological structures of biological soft tissues, including tumors. In this study, we quantitatively compared phase-contrast synchrotron-based X-ray computed tomography images and images of histopathological hematoxylin-eosin-stained sections of spontaneously occurring rat testicular tumors that contained different types of cells. The absolute densities measured on the phase-contrast synchrotron-based X-ray computed tomography images correlated well with the densities of the nuclear chromatin in the histological images, thereby demonstrating the ability of phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer to reliably identify the characteristics of cancer cells within solid soft tissue tumors. In addition, 3-dimensional synchrotron-based phase-contrast X-ray computed tomography enables screening for different structures within tumors, such as solid, cystic, and fibrous tissues, and blood clots, from any direction and with a spatial resolution down to 26 μm. Thus, phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer shows potential for being useful in preclinical cancer research by providing the ability to depict the characteristics of tumor cells and by offering 3-dimensional information capabilities.

A new species of anapid spider (Araneae: Araneoidea, Anapidae) in Eocene Baltic amber, imaged using phase contrast X-ray computed micro-tomography

Zootaxa ◽  
2011 ◽  
Vol 2742 (1) ◽  
pp. 60 ◽  
Author(s):  
DAVID PENNEY ◽  
ANDREW MCNEIL ◽  
DAVID I. GREEN ◽  
ROBERT BRADLEY ◽  
YURI M. MARUSIK ◽  
...  
Keyword(s):  
New Species ◽  
Phase Contrast ◽  
Baltic Amber ◽  
X Ray ◽  
The Family ◽  
X Ray Computed ◽  
Micro Tomography ◽  
The Baltic ◽  
A New Species ◽  
Eocene Amber

A new species of the extant spider family Anapidae is described from a fossil mature male in Eocene amber from the Baltic region and tentatively assigned to the genus Balticoroma Wunderlich, 2004. Phase contrast X-ray computed micro-tomography was used to reveal important features that were impossible to view using traditional microscopy. Balticoroma wheateri new species is easily diagnosed from all other anapids by having clypeal extensions that run parallel to the ectal surface of the chelicerae and in having the metatarsus of the first leg highly reduced and modified into what is presumably a y-shaped clasping structure. Although only a single extant anapid species occurs in northern Europe, the family was diverse in the Eocene. The discovery of yet another anapid species in Baltic amber supports the idea that Eocene European forests may have been a hotspot of evolution for this family of spiders.

Three-dimensional analysis of plant structure using high-resolution X-ray computed tomography

2003 ◽  
Vol 8 (1) ◽  
pp. 2-6 ◽  
Author(s):  
Wolfgang H Stuppy ◽  
Jessica A Maisano ◽  
Matthew W Colbert ◽  
Paula J Rudall ◽  
Timothy B Rowe
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Dimensional Analysis ◽  
Three Dimensional ◽  
Plant Structure ◽  
X Ray ◽  
X Ray Computed

Three dimensional characterization of laser ablation craters using high resolution X-ray computed tomography

2018 ◽  
Vol 139 ◽  
pp. 75-82 ◽  
Author(s):  
A.H. Galmed ◽  
A. du Plessis ◽  
S.G. le Roux ◽  
E. Hartnick ◽  
H. Von Bergmann ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Laser Ablation ◽  
High Resolution ◽  
Three Dimensional ◽  
X Ray ◽  
X Ray Computed
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