Effect of Motion on the Sonographic and Magnetic Resonance Patterns of Ageing Blood

1986 ◽  
Vol 27 (4) ◽  
pp. 455-458 ◽  
Author(s):  
A. Alanen ◽  
P. Nummi

The sonographic appearance of a hematoma may be affected by various factors, including the age of the hematoma. The effect of mechanical motion on the echogenicity and histologic appearance, and on the proton relaxation times T1 and T2 of blood clots, was studied in vitro for up to 21 days. All clots were of similar echogenicity and microscopic appearance during the first 2 days. The minimally disturbed clots were sonolucent from day 4 onwards, whereas moderate mechanical disturbance changed the microscopic structure of the blood clots and caused them to retain their echogenicity. Proton relaxation times T1 and T2 of both minimally disturbed and vigorously manipulated blood samples showed a rapid shortening of T1 and a less marked decrease of T2 between days 1 and 4, which was independent of mechanical motion. The ultrasonic appearance reflected the histologic appearance but not necessarily the age of the clot. The magnetic resonance (MR) parameters T1 and to a lesser extent T2 accurately reflected the age of the clot during the first 6 days. Although relatively gentle motion caused a large change in the ultrasonic appearance of the clots, vigorous shaking did not affect the magnetic resonance appearance of human blood clots.

2021 ◽  
Vol 12 (4) ◽  
pp. 5174-5186

To work with cancer cell cultures in vitro at 1.5 Tesla Magnetic Resonance Imaging (MRI), it was necessary to develop dedicated receiver coils. This device allowed to adjust the shape of the tested objects and thus improve the quality of imaging. One of the conditions for this new device was to increase the recorded signal level and reduce the distance between the tested object and the receiving elements of the coil. MCF7 (breast adenocarcinoma, Her-2 positive), ACHN (kidney cancer cells), and A549 (lung cancer cells) were characterized by using magnetic resonance imaging (MRI) in vitro. MRI measurements were performed using the clinical scanner with a 1.5 Tesla magnetic field. MCF-7, ACHN, and A549 cancer cells were characterized by T1 and T2 relaxation times. For MCF-7 cells, the relaxation times T1 and T2 were 2360 ± 12 ms and 116 ± 0.9 ms, respectively. For ACHN cells, the relaxation times T1 and T2 were 1354 ± 193 ms and 80 ± 9 ms, respectively. Values of T1 and T2 for A549 cancer cells cultures were 1527 ± 59 ms and 150 ± 8 ms, respectively. Once an accurate pulse protocol has been established and satisfactory reproducibility was obtained, the determination of relaxation times can be used as a tool to monitor cancer cell cultures using MRI in vitro based on the determination of changes in relaxation times.


1992 ◽  
Vol 33 (3) ◽  
pp. 266-270 ◽  
Author(s):  
P. Naeser ◽  
K. Å. Thuomas ◽  
A. Roberto ◽  
B. S. Larsson

The short proton relaxation times in the MR images of malignant melanomas make them different from most other tumors. We have previously shown that the T1 and T2 signals may be significantly influenced when the water distribution of the tumor is changed in vivo and in vitro. In the present work T1 and T2 were estimated and compared with the electron microscopy picture in subcutaneously implanted B16 melanomas in mice. Two hours after the mice were given an i.p. injection of 0.9% NaCl containing 10% glucose and 10% fructose (9 mice) both the T2 components were markedly and the T1 slightly prolonged. At the same time the electron microscopy picture displayed swelling of the melanocytes together with a marked decrease in number and size of their mitochondriae. There were no changes in the MR image or the melanocyte structure in control mice injected with 0.9% NaCl (9 mice) or 0.9% NaCl containing 10% fructose. It is concluded that the changed MR image may be coupled to the metabolism in melanoma.


1987 ◽  
Vol 28 (3) ◽  
pp. 345-351 ◽  
Author(s):  
L. Kjær ◽  
C. Thomsen ◽  
O. Henriksen ◽  
P. Ring ◽  
M. Stubgaard ◽  
...  

Several circumstances may explain the great variation in reported proton T1 and T2 relaxation times usually seen. This study was designed to evaluate the accuracy of relaxation time measurements by magnetic resonance imaging (MRI) operating at 1.5 tesla. Using a phantom of nine boxes with different concentrations of CuSO4 and correlating the calculated T1 and T2 values with reference values obtained by two spectrometers (corrected to MRI-proton frequency=64 MHz) we found a maximum deviation of about 10 per cent. Measurements performed on a large water phantom in order to evaluate the homogeneity in the imaging plane showed a variation of less than 10 per cent within 10 cm from the centre of the magnet in all three imaging planes. Changing the gradient field strength apparently had no influence on the T2 values recorded. Consequently diffusion processes seem without significance. It is concluded that proton T1 and T2 relaxation times covering the majority of the biologic range can be measured by MRI with an overall accuracy of 5 to 10 per cent. Quality control studies along the lines indicated in this study are recommended.


1986 ◽  
Vol 64 (5) ◽  
pp. 795-802 ◽  
Author(s):  
Joshua B. Bederson ◽  
Henry M. Bartkowski ◽  
Kirkland Moon ◽  
Meredith Halks-Miller ◽  
Merry C. Nishimura ◽  
...  

✓ Many aspects of the use of high-resolution nuclear magnetic resonance (NMR) imaging in the examination of brain edema have not been fully explored. These include the quantitation of edema fluid, the ability to distinguish between various types of edema, and the extent to which tissue changes other than a change in water content can affect NMR relaxation times. The authors have compared NMR relaxation times obtained by both in vivo magnetic resonance imaging (MRI) and in vitro NMR spectroscopy of brain-tissue samples from young adult rats with cold lesions, fluid-percussion injury, hypoxic-ischemic injury, bacterial cerebritis, and cerebral tumor. Changes in relaxation times were compared with changes in brain water content, cerebral blood volume, and the results of histological examination. In general, both in vivo and in vitro longitudinal relaxation times (T1) and transverse relaxation times (T2) were prolonged in the injured hemispheres of all experimental groups. Water content of tissue from the injured hemispheres was increased in all groups. A linear correlation between T2 (but not T1) and water content was found. Changes in the values of T1 and T2 could be used to distinguish tumor from cold-injured tissue. Cerebral blood volume was reduced in the injured hemispheres and correlated inversely with prolongation of T1 and T2. The results of this study suggest that, in a clinical setting, prolongation of T2 is a better indicator of increased water content than prolongation of T1, yet quantitation of cerebral edema based solely upon prolongation of in vivo or in vitro T1 and T2 should be undertaken with caution.


2000 ◽  
Vol 3 (06) ◽  
pp. 498-508 ◽  
Author(s):  
Songhua Chen ◽  
D.T. Georgi ◽  
Oscar Olima ◽  
Hector Gamin ◽  
J.C. Minetto

Summary We report a case study of using nuclear magnetic resonance (NMR) multiple-te, dual wait-time (tw) log acquisitions for quantitative characterization of San Jorge Basin reservoir oil viscosity. Previously, dual-tw logs have been used to discern gas and oil from water, while dual-te logs have been used as a qualitative light oil indicator. Although theoretically simple, quantitative determination of viscosity from dual-te logs is complicated by several factors, including poor signal-to-noise ratio, difficulties in separating oil from water, and the uncertainty of internal gradient strength. In the present study, multiple-te acquisitions of dual-tq logs were used to isolate the oil from the water signal. The values of viscosity of the reservoir fluids can be estimated from either intrinsic T2 or T1. In estimation of the apparent T2, we used a model that does not explicitly require knowledge of the internal gradient, thereby minimizing the effects arising from the uncertainty of the internal and tool gradient strengths. Because T1 and intrinsic T2 are estimated independently, the degree of agreement between the two values provides an indication of the reliability of the two estimates. The main example in the study of four pay zones was thought to contain viscous oil. However, our analysis indicated that the viscosity values of the oil are less than 5 cP. The predictions have been substantiated by production of light hydrocarbons from the three zones that have been perforated. Further, a good agreement is obtained for the viscosity estimates based on NMR log data and laboratory pressure/volume/temperature (PVT) analysis. Introduction Hydrocarbon viscosity is an important reservoir fluid parameter that significantly affects oil recovery and economics. Fluid flow is inversely proportional to viscosity and the higher the viscosity the lower the flow rate and the slower the recovery. Further, when two or more fluids are flowing, the ratio of the viscosities, the mobility ratio, is one of the key parameters that affects sweep efficiency and ultimate recovery. In many reservoirs, it is uneconomical to produce heavy, viscous oil, and thus it is crucial to determine oil viscosity before completing the well. The problem is even more pressing when oil viscosities vary within a hydrocarbon column or from zone to zone when attempting to commingle multiple zones. Many laboratory procedures can determine viscosity. Samples for viscosity determination may be obtained from reservoir fluid samples recovered from well tests or drill stem tests, downhole fluid samplers, or reconstituted from separator samples. Sampling procedures are generally limited to a few depths; and, of course, samples reconstituted from separators are associated with the entire producing interval and may not be associated with a single depth. Further, there is always the concern that the fluid samples may not be representative of the in-situ reservoir fluids. Nuclear magnetic resonance (NMR) logging measurements have the potential to provide in-situ viscosity measurement because the NMR relaxation times, T1 and T2, correlate strongly with fluid viscosity. The difficulty with NMR-determined viscosity is that the measurement is relatively shallow. Thus, the hydrocarbon saturations may be significantly reduced (So or Sor), and hence, the sought NMR oil signal is small. Further, the interpretation may be complicated by NMR signals originating from the invading fluids. NMR response is controlled by both rock and fluid properties. In fact, NMR log interpretation is complicated because it is not always clear whether the T2 decay reflects hydrocarbon and/or rock properties. However, we are fortunate because two NMR experiment parameters, te and tw (Fig. 1), can be used to tailor the NMR data acquisition to separate the hydrocarbon and rock property effects. One of the first specialized NMR applications that took advantage of the ability to control NMR log acquisition parameters was hydrocarbon typing.1 NMR hydrocarbon typing in porous media relies on the difference in NMR response in either or both of the relaxation times (T1 and T2) or diffusivity of oil, water, and gas. By carefully designing the logging program and using combinations of pulse sequences, one can enhance the relaxation and diffusivity contrasts between the different fluid phases. The two commonly used approaches for magnetic resonance image log (MRIL®)** based hydrocarbon typing are dual-tq logging1,2 and dual-te logging.3,4 Dual-tw logging utilizes the T1 contrast between nonwetting light hydrocarbons and the wetting water for quantitative light hydrocarbon typing, while dual-te logging utilizes the viscosity (and thus diffusivity) contrast between reservoir fluids. The latter, to our knowledge, previously has been used mainly as a qualitative, or, at best semiquantitative, hydrocarbon indicator. Quantitative estimation of oil viscosity and saturation require solving problems related to uncertainty of internal magnetic-field gradient and separating oil from water signals. We combined the dual-tw and dual-te approaches to maximize the advantages of both T1 and T2 contrasts. With multiple-te passes of dual-tw logs, we are able to eliminate a majority of the water signal from dual-tw logs; the remaining signal is predominantly an oil signal. The characterization of oil viscosity is achieved by analyzing the relaxation times and diffusion effect on the isolated oil signal with the multiple-te data acquisition. This reduces uncertainties due to the interfering water signal originating from either the irreducible and bound water or from the invading mud filtrate present on conventional dual-te logs.


2016 ◽  
Vol 78 (5) ◽  
pp. 1724-1733 ◽  
Author(s):  
Benedikt Rieger ◽  
Fabian Zimmer ◽  
Jascha Zapp ◽  
Sebastian Weingärtner ◽  
Lothar R. Schad

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