scholarly journals Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury

2020 ◽  
Vol 48 (4) ◽  
pp. 030006052091959
Author(s):  
Guobing Chen ◽  
Dandan Ge ◽  
Bizhen Zhu ◽  
Huixuan Shi ◽  
Qilin Ma
Keyword(s):  
Weight Ratio ◽  
Dry Weight ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Permeability Index ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Metalloproteinase 9 ◽  
Necrosis Factor ◽  
Pulmonary Permeability ◽  
Serum Tumor Necrosis

Objective This study aimed to examine the changes and significance of matrix metalloproteinase 9 (MMP9), MMP2, tissue inhibitor of metalloproteinase 1 (TIMP1), and TIMP2 in rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI). Methods Wistar rats were randomly divided into a control group (injected with saline) and an ALI group (injected with LPS), then subdivided into four time points (2, 6, 12, and 24 hours). Serum tumor necrosis factor alpha and interleukin-6 levels were detected by ELISA to investigate the inflammatory reaction after LPS injection. The degree of ALI was determined by hematoxylin–eosin staining of lung tissue, the lung wet/dry weight ratio, and pulmonary permeability index. Changes in lung MMP and TIMP protein and mRNA levels were detected by western blotting and quantitative real-time polymerase chain reaction. Results Changes in the ratios of MMP9/TIMP1 and MMP2/TIMP2 were consistent with and strongly positively associated with the lung wet/dry weight ratio, the pulmonary permeability index, and serum tumor necrosis factor alpha and interleukin-6 levels in the ALI group. Conclusion ALI induced by LPS may be related to upregulation of MMP9/TIMP1 and MMP2/TIMP2 ratios.

MANIPULATION OF ENDOGENOUS ADENOSINE MODULATES SERUM TUMOR NECROSIS FACTOR-ALPHA (TNF-α) DURING SEPSIS IN RATS

Shock ◽  
1999 ◽  
Vol 11 (Supplement) ◽  
pp. 25
Author(s):  
A D Sam ◽  
H. Barcino ◽  
A C Sharma ◽  
H B Bosmann ◽  
J L Ferguson ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Necrosis Factor Alpha ◽  
Endogenous Adenosine ◽  
Tnf Α ◽  
Factor Alpha ◽  
Necrosis Factor ◽  
Serum Tumor Necrosis

scholarly journals Severe imported malaria in a Serbian referral center

2019 ◽  
Vol 76 (5) ◽  
pp. 470-475
Author(s):  
Jasmina Poluga ◽  
Uros Karic ◽  
Zorica Dakic ◽  
Natasa Katanic ◽  
Lidija Lavadinovic ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Severe Malaria ◽  
Disease Severity ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
World Health ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor ◽  
Serum Tumor Necrosis

Background/Aim. The World Health Organization estimates that 3.2 billion people are at a risk of being infected with malaria. Thus, the adequate diagnostic protocols for malaria, especially those aimed at determining disease severity, are paramount both in endemic and non-endemic setting. The aim of this study was to identify the demographic, parositological, clinical and laboratory characteristics associated with severe malaria in a non-endemic settings. Methods. We analyzed 22 patients with severe malaria and compared their clinical and laboratory findings with those of the patients with non-severe malaria in a search of predictors of disease severity. All patients were treated at the Infectious and Tropical Diseases University Hospital, Clinical Centre of Serbia in Belgrade, Serbia from 2000 to 2010. Results. The average age of patients with with severe malaria was 44.86 ? 12.33 years and men predominated (95.45%). The patients with severe malaria were infected Plasmodium falciparum (P. falciparum) significantly more frequently compared with those with non-severe disease (p =0.047). Jaundice was the most commonly observed feature of severe malaria, followed by anemia and renal failure. The multifactor analysis of variance showed that thrombocytopenia (p = 0.05) and high serum tumor necrosis factor-alpha levels (p = 0.02) were significantly associated with the disease severity. Conclusion. A high index of suspicion for malaria should be maintained when evaluating febrile patients returning from the malaria endemic regions. The elevated serum tumor necrosis factor-alpha levels and thrombocytopenia are associated with severe malaria in non-endemic settings.

Differential Effects of Corticosteroids on The Expression of Cyclooxygenase-2, Tumour Necrosis Factor-Alpha and Matrix Metalloproteinase-9 in An Animal Model of Migraine

Cephalalgia ◽  
2008 ◽  
Vol 28 (11) ◽  
pp. 1179-1187 ◽  
Author(s):  
G-M Kim ◽  
K-S Jin ◽  
C-S Chung
Keyword(s):  
Animal Model ◽  
Inflammatory Mediators ◽  
Tumour Necrosis ◽  
Tumour Necrosis Factor Alpha ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Cox 2 ◽  
Metalloproteinase 9 ◽  
Necrosis Factor

Nitric oxide (NO) directly activates trigeminal afferents innervating the dura mater and up-regulates inflammatory mediators. We evaluated NO-mediated up-regulation of cyclooxygenase-2 (COX-2), tumour necrosis factor-alpha (TNF-α) and matrix metalloproteinase-9 (MMP-9), and the effect of glucocorticoid administration in an experimental animal model of migraine. COX-2 and TNF-α expression and MMP-9 activity were increased after continuous intravenous infusion of glyceryl trinitrate (GTN), a NO donor. Immunofluorescence staining demonstrated strong expression of these inflammatory mediators in the meningeal blood vessels. Methylprednisolone (MP) down-regulated MMP-9, which was reversed by RU486, a glucocorticoid receptor antagonist. COX-2 and TNF-α expression was not affected by MP or RU486 administration. These results suggest proinflammatory mediators are involved in the NO-mediated cascade of migraine pathogenesis. Further understanding of the activation of these inflammatory mediators at the transcriptional level may have therapeutic implications for future migraine treatments.

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