Immunohistochemical Characterization of Hemangiopericytomas and Other Spindle Cell Tumors in the Dog

1996 ◽  
Vol 33 (4) ◽  
pp. 391-397 ◽  
Author(s):  
J. Pérez ◽  
M. J. Bautista ◽  
E. Rollón ◽  
F. Chacón-M. de Lara ◽  
L. Carrasco ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Glial Fibrillary Acidic Protein ◽  
Factor Viii ◽  
Spindle Cell ◽  
Acidic Protein ◽  
Muscle Actin ◽  
Factor Viii Related Antigen ◽  
S 100 ◽  
Spindle Cell Tumors ◽  
And Control

The immunohistochemical expression of muscle actin has been studied in 45 canine hemangiopericytomas (CHP) using a monoclonal antibody (HHF35) and formalin-fixed, paraffin-embedded specimens. The distribution of vimentin, desmin, cytokeratins, lysozyme, factor VIII-related antigen, S-100 protein, and glial fibrillary acidic protein was studied both in CHP and in some canine soft-tissue neoplasms (seven fibrosarcomas, seven benign schwannomas, seven benign fibrous histiocytomas, and six leiomyosarcomas) used as controls for differential diagnosis. All CHP and control tumors expressed vimentin. Twenty-three CHP expressed muscle actin, whereas all control tumors analyzed were muscle actin-negative, with the exception of leiomyosarcomas. Among muscle actin- and vimentin-positive CHP, one case could be reclassified as leiomyosarcoma because it was desmin-positive, two cases expressed lysozyme, and nine cases expressed S-100 protein. Among muscle actin-negative and vimentin-positive CHP, seven expressed S-100 protein. In addition, S-100 protein was detected in five schwannomas. All CHP and control tumors analyzed were negative for cytokeratins, factor VIII-related antigen, and glial fibrillary acidic protein. Our results support the hypothesis of a pericytic origin of CHP, and suggest that muscle actin, desmin, vimentin, and lysozyme could be useful for the differential diagnosis of canine spindle cell tumors, but not all these neoplasms can be identified with these tumor tissue markers.

Best Practices in Diagnostic Immunohistochemistry: Pleomorphic Cutaneous Spindle Cell Tumors

2007 ◽  
Vol 131 (10) ◽  
pp. 1517-1524 ◽  
Author(s):  
Andrew L. Folpe ◽  
Kumarasen Cooper
Keyword(s):  
Differential Diagnosis ◽  
Best Practices ◽  
English Language ◽  
Spindle Cell ◽  
Smooth Muscle Actin ◽  
S100 Protein ◽  
Muscle Actin ◽  
Spindle Cell Tumors ◽  
Immunohistochemical Studies ◽  
Language Literature

Abstract Context.—Pleomorphic cutaneous spindle cell tumors can be difficult to distinguish solely on histologic grounds. The use of ancillary immunohistochemical studies can greatly assist in this differential diagnosis. Objective.—To review histologic and immunohistochemical aspects of cutaneous spindle cell tumors and discuss a basic panel of markers to assist in the differential diagnosis. Data Sources.—English-language literature published between 1981 and 2005. Conclusions.—A basic immunohistochemistry panel for high-molecular-weight cytokeratin, melanocytic markers (S100 protein, HMB-45, Melan-A), smooth muscle actin, desmin, and endothelial markers (CD31, CD34) is effective in diagnosing most cutaneous spindle cell tumors.

Male Breast Cellular Myofibroblastoma With a Rich Reticulinic Network

2012 ◽  
Vol 6 (4) ◽  
pp. 344-348 ◽  
Author(s):  
Simona Gurzu ◽  
Ioan Jung
Keyword(s):  
Estrogen Receptor ◽  
Differential Diagnosis ◽  
Smooth Muscle ◽  
Factor Viii ◽  
Tumor Cells ◽  
Surgical Excision ◽  
Morphological Features ◽  
Factor Viii Related Antigen ◽  
Spindle Cells ◽  
S 100

Myofibroblastoma is a rare mesenchymal tumor located not only in breast but also in extramammary sites. This is the 79th case of breast myofibroblastoma reported in the literature. This tumor presents a great variety of morphological features, which increase the difficulty of differential diagnosis. The authors report a breast myofibroblastoma diagnosed in a 73-year-old male. The article discusses a bilateral gynecomastia and a palpable right breast well-defined tumor, without calcifications on mammography, which was surgically removed. Macroscopically, a well-circumscribed uncapsulated nodule was seen with lobular arrangement on cut section, and microscopically, a nodule with pushing borders and a connective pseudocapsule was seen. The spindle cells were arranged in fascicular clusters, with focally collagen bundles and a rich reticulinic network stained black with Gömöri impregnation. Immunohistochemically, the tumor cells were marked by vimentin, CD34, desmin, and smooth muscle antigen and did not express cytokeratin, S-100 protein, CD99, CD10, and factor VIII–related antigen. More than 90% of the cells expressed estrogen receptor. No recurrences were reported 2 years after surgical excision. This case is a variant of cellular myofibroblastoma, with a rich reticulinic network and scanty collagen bands.

Expression of glial fibrillary acidic protein, actin, fibronectin and factor VIII antigen in human astrocytomas

Pathology ◽  
1983 ◽  
Vol 15 (4) ◽  
pp. 373-378 ◽  
Author(s):  
Stephen J. Lolait ◽  
J.H. Harmer ◽  
G. Auteri ◽  
J.S. Pedersen ◽  
B.H. Toh
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Factor Viii ◽  
Acidic Protein ◽  
Factor Viii Antigen ◽  
Human Astrocytomas

Developmental Expression of Glial Fibrillary Acidic Protein and Actin-Encoding Messages in Quaking and Control Mice

1992 ◽  
Vol 14 (5-6) ◽  
pp. 351-356 ◽  
Author(s):  
H. Chen ◽  
P. Sun ◽  
E. Parmantier ◽  
F. Cabon ◽  
P. Dupouey ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
Developmental Expression ◽  
And Control

scholarly journals Glial Fibrillary Acidic Protein (GFAP) in Oligodendrogliomas: A Reflection of Transient GFAP Expression by Immature Oligodendroglia

1986 ◽  
Vol 13 (4) ◽  
pp. 307-311 ◽  
Author(s):  
V. Jagadha ◽  
W.C. Halliday ◽  
L.E. Becker
Keyword(s):  
White Matter ◽  
Glial Fibrillary Acidic Protein ◽  
Tumor Cells ◽  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Immunohistochemical Staining ◽  
Neuron Specific Enolase ◽  
Acidic Protein ◽  
Gfap Expression ◽  
S 100

ABSTRACT:Fourteen pure oligodendrogliomas were studied by light- and electronmicroscopy and immunohistochemistry to examine glial fibrillary acidic protein (GFAP) positivity in the tumors. To compare the immunohistochemical staining patterns of neoplastic oligodendroglia and immature oligodendroglia, myelination glia in the white matter of eight normal brains from children under 6 months of age were studied. The tumors possessed light microscopic and ultrastructural features characteristic of oligodendrogliomas. Microtubules were found in the cytoplasm of nine tumors on electronmicroscopy. In one, intermediate filaments and microtubules were observed in occasional tumor cells with polygonal crystalline structures in the cytoplasm. Using the peroxidase-antiperoxidase technique, all specimens were stained for GFAP, vimentin, S-100 and neuron-specific enolase (NSE). In nine tumors, variable numbers of cells with an oligodendroglial morphology reacted positively for GFAP. All tumors were positive for S-100 and negative for vimentin and NSE. The myelination glia in the eight normal brains stained positively for GFAP but not for vimentin. Vimentin is expressed by developing, reactive and neoplastic astrocytes. Thus, GFAP positivity combined with vimentin negativity in both neoplastic and immature oligodendroglia suggests that GFAP positivity in oligodendrogliomas may reflect the transient expression of this intermediate filament by immature oligodendroglia.

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