Flow cytometric DNA analysis in conservatively treated renal tumours

1995 ◽  
Vol 62 (1_suppl) ◽  
pp. 141-143
Author(s):  
P. Beltrami ◽  
M. Lazzarotto ◽  
G. Giusti ◽  
C. Tallarigo ◽  
G. Malossini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Analysis ◽  
Tumour Size ◽  
Prognostic Significance ◽  
Dna Flow Cytometry ◽  
Additional Parameter ◽  
Flow Cytometric ◽  
Renal Tumours ◽  
Dna Histograms

— The DNA histograms of 21 conservatively resected renal tumours were studied using DNA flow cytometry. Five patients had an imperative and sixteen an elective indication for conservative resection of the renal tumour. On the basis of DNA histograms twelve aneuploid tumours were pointed out. A mean follow-up of 34.2 months was considered to see whether the ploidy would provide criteria with a prognostic significance, to be useful as an additional parameter. None of the twenty-one patients had local recurrence or distant metastasis: in our series the DNA analysis had no influence on the prognosis of this group of patients. The tumour size seems to be the only selective parameter for choosing renal-conserving surgery.

L'analisi del DNA mediante citometria a flusso nei tumori superficiali della vescica: DNA analysis through flow cytometry in superficial bladder tumours

1995 ◽  
Vol 62 (2) ◽  
pp. 188-195
Author(s):  
R. Pianon ◽  
A. D'Amico ◽  
M. Rahmati ◽  
A. Paganelli ◽  
D. Schiavone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Clinical Practice ◽  
Dna Analysis ◽  
Prognostic Significance ◽  
Clinical Development ◽  
Dna Flow Cytometry ◽  
Through Flow ◽  
Data Investigation ◽  
Precise Role ◽  
Bladder Tumours

The aim of this work is to try to characterize the prognostic value of DNA flow cytometry in superficial bladder tumours and to try to indicate this investigation in clinical practice. The Authors review results reported in literature and those relative to their experience. Data investigation shows that this test has prognostic significance because it is correlated with the stage and grade of the tumour. The correlation with the clinical development of the tumour is not clear in the Authors’ experience. The Authors think that flow cytometry does not have a precise role in clinical practice.

The Role of Flow Cytometric Dna Analysis in Renal Tumours

1992 ◽  
Vol 59 (6) ◽  
pp. 21-24 ◽  
Author(s):  
G. Mobilio ◽  
G. Bianchi ◽  
N. Franzolin ◽  
P. Beltrami ◽  
M. Lazzarotto
Keyword(s):  
Dna Analysis ◽  
Needle Aspiration ◽  
Tumour Tissue ◽  
Additional Parameter ◽  
Aneuploid Cell ◽  
Tumour Mass ◽  
Tissue Samples ◽  
Fine Needle Aspirates ◽  
Flow Cytometric ◽  
Renal Tumours

The results obtained with flow cytometric (FCM) DNA analysis in renal tumours are variable, since not all investigators have demonstrated a statistically significant correlation between presence of aneuploid cell populations and patient survival. The most important reason is probably the different number of samples analysed for each tumour. Therefore most investigators agree that it is essential to perform flow cytometry analysis on a large number of tumour tissue samples. In our study we decided to do the FCM DNA analysis on multiple tissue samples and on needle aspiration samples drawn from the surgically removed tumour mass. Our data showed 44% aneuploid tumours in tissue samples and 58% aneuploidies in needle aspirates. We think that FCM DNA analysis on fine needle aspirates can detect an aneuploid population at least as frequently as on tissue samples. Therefore this technique could substitute multiple tumour tissue sampling. At present FCM in renal tumours seems to be a parameter for the identification of patients at high risk for progression, who should receive adjuvant therapy or treatment for recurrency. What is more, pre-operative FCM on needle aspirates could provide an important additional parameter in the choice of conservative-type surgical treatment, since the only criterion currently available is the size of the tumour.

scholarly journals CD161 Is Expressed in a Subset of T-Cell Prolymphocytic Leukemia Cases and Is Useful for Disease Follow-up

2019 ◽  
Vol 152 (4) ◽  
pp. 471-478
Author(s):  
Scott R Gilles ◽  
Sophia L Yohe ◽  
Michael A Linden ◽  
Michelle Dolan ◽  
Betsy Hirsch ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Nk Cells ◽  
Retrospective Review ◽  
T Cell Subsets ◽  
Prolymphocytic Leukemia ◽  
Flow Cytometry Data ◽  
Flow Cytometric ◽  
Flow Cytometric Evaluation

AbstractObjectivesCD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.MethodsRetrospective review of T-PLL cases with complete flow cytometry data including CD161.ResultsWe identified 10 cases of T-PLL with flow cytometric evaluation of CD161 available. Six of these cases were positive for CD161 expression. All CD161-positive cases were positive for CD8 with variable CD4 expression, whereas all CD161-negative cases were negative for CD8. In a case with two neoplastic subsets positive and negative for CD8, only the former expressed CD161.ConclusionsThese novel results suggest that CD161 is often aberrantly expressed in a defined subset of T-PLL positive for CD8. We are showing the utility of this immunophenotype in diagnosis and follow-up.

scholarly journals Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.

1983 ◽  
Vol 31 (11) ◽  
pp. 1333-1335 ◽  
Author(s):  
D W Hedley ◽  
M L Friedlander ◽  
I W Taylor ◽  
C A Rugg ◽  
E A Musgrove
Keyword(s):  
Flow Cytometry ◽  
Retrospective Study ◽  
Clinical Outcome ◽  
Dna Content ◽  
Prognostic Significance ◽  
Distilled Water ◽  
Archival Material ◽  
Cellular Dna ◽  
Pathological Material ◽  
Dna Histograms

A method has been developed that allows flow cytometry to be used for measuring the cellular DNA content of paraffin-embedded human tumors. Thick (i.e., 30 micron) sections were cut from tissue blocks using a microtome and dewaxed in xylene. The sections were then rehydrated by sequentially immersing them in 100, 95, 70, and 50% ethanol before finally washing in distilled water. Single cell suspensions were then prepared by incubation in 0.5% pepsin, pH 1.5, at 37 degrees C for 30 min. The cells were counted, washed, and stained with 1 microgram/ml 4',6'-diamidino-2-phenylindole for 30 min, and DNA content was measured using an ICP 22 flow cytometer. There was a good correlation between the DNA histograms produced using this method and those obtained using unfixed tissue from the same tumor stained with ethidium bromide plus mithramycin. This method allows the retrospective study of archival material where the clinical outcome is already known, and it should, therefore, be particularly useful for determining the prognostic significance of abnormal DNA content measured by flow cytometry.

Flow cytometric DNA analysis of human solid tumors: A review of the interpretation of DNA histograms☆

1991 ◽  
Vol 22 (11) ◽  
pp. 1085-1098 ◽  
Author(s):  
Robert P. Wersto ◽  
Roberta L. Liblit ◽  
Leopold G. Koss
Keyword(s):  
Solid Tumors ◽  
Dna Analysis ◽  
Flow Cytometric ◽  
Human Solid Tumors ◽  
Dna Histograms

Follow-up on flow cytometric DNA analysis of squamous cell carcinoma of the tongue

1989 ◽  
Vol 157 (4) ◽  
pp. 377-380 ◽  
Author(s):  
William B. Farrar ◽  
Brenda J. Sickle-Santanello ◽  
Sedigheh Keyhani-Rofagha ◽  
Jane F. DeCenzo ◽  
Robert V. O'toole
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Dna Analysis ◽  
Flow Cytometric ◽  
Carcinoma Of The Tongue

Diagnosis and risk stratification of Barrett’s dysplasia by flow cytometric DNA analysis of paraffin-embedded tissue

Gut ◽  
2017 ◽  
Vol 67 (7) ◽  
pp. 1229-1238 ◽  
Author(s):  
Won-Tak Choi ◽  
Jia-Huei Tsai ◽  
Peter S Rabinovitch ◽  
Thomas Small ◽  
Danning Huang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Risk Stratification ◽  
Dna Content ◽  
Dna Analysis ◽  
Diagnostic Marker ◽  
Oesophageal Adenocarcinoma ◽  
Dna Flow Cytometry ◽  
Low Grade ◽  
Area Of Interest ◽  
Formalin Fixed Paraffin Embedded

ObjectiveThe diagnosis of dysplasia in Barrett’s oesophagus (BO) can be challenging, and reliable ancillary techniques are not available. This study examines if DNA content abnormality detected by flow cytometry can serve as a diagnostic marker of dysplasia and facilitate risk stratification of low-grade dysplasia (LGD) and indefinite for dysplasia (IND) patients using formalin-fixed paraffin-embedded (FFPE) BO samples with varying degrees of dysplasia.DesignDNA flow cytometry was performed on 80 FFPE BO samples with high-grade dysplasia (HGD), 38 LGD, 21 IND and 14 negative for dysplasia (ND). Three to four 60-micron thick sections were cut from each tissue block, and the area of interest was manually dissected.ResultsDNA content abnormality was identified in 76 HGD (95%), 8 LGD (21.1%), 2 IND (9.5%) and 0 ND samples. As a diagnostic marker of HGD, the estimated sensitivity and specificity of DNA content abnormality were 95% and 85%, respectively. For patients with DNA content abnormality detected at baseline LGD or IND, the univariate HRs for subsequent detection of HGD or oesophageal adenocarcinoma (OAC) were 7.0 and 20.0, respectively (p =<0.001).ConclusionsThis study demonstrates the promise of DNA flow cytometry using FFPE tissue in the diagnosis and risk stratification of dysplasia in BO. The presence of DNA content abnormality correlates with increasing levels of dysplasia, as 95% of HGD samples showed DNA content abnormality. DNA flow cytometry also identifies a subset of patients with LGD and IND who are at higher risk for subsequent detection of HGD or OAC.

Prognostic significance of nuclear DNA analysis by flow cytometry in the progression of superficial bladder cancer

1997 ◽  
Vol 64 (1) ◽  
pp. 75-76
Author(s):  
M. De Siati ◽  
D. Grassi ◽  
M. Lazzarotto ◽  
N. Franzolin ◽  
M. Saugo ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Nuclear Dna ◽  
Superficial Bladder Cancer ◽  
Prognostic Significance ◽  
Strongly Correlated ◽  
Tumour Stage ◽  
Dna Index ◽  
T1 Stage ◽  
Bladder Tumours

The authors used nuclear DNA analysis to evaluate 142 patients with bladder tumours in stage Ta, T1. During follow-up, only the T1G2-G3 forms progressed. Tumour stage and DNA Index (DI) > 1.25 were strongly correlated to tumour invasiveness. According to these data, patients with tumours in the T1 stage and Dl>1.25 should undergo closer follow-up.

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