Analysis of Omega-3 Fatty Acid Content in Fish Oil Products

2021 ◽  
pp. 088307382110150
Author(s):  
Eileen D. Ward ◽  
Katelyn Thomasson ◽  
K. Robin Fischer
Keyword(s):  
Fish Oil ◽  
Fatty Acid Content ◽  
Oil Products ◽  
Search Term ◽  
Omega 3 ◽  
Omega 3 Fatty Acid ◽  
Epa And Dha ◽  
Serving Sizes ◽  
Serving Size ◽  
Cardiovascular Benefit

Background: Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosehexaenoic acid (DHA), often found in fish oil supplements, have been linked to cardiovascular benefits in proper doses. Objectives: Quantify serving sizes and EPA and DHA content of fish oil products and determine which products contain appropriate amounts of EPA and DHA per serving to lower cholesterol. Methods: Products were identified through the National Institutes of Health’s Dietary Supplement Label Database using the search term “fish oil.” Product labels were reviewed for EPA and DHA content. The number of units, such as capsules, gummies, or milliliters, necessary to obtain a total of at least 2,000 mg of EPA and DHA was also evaluated. Descriptive statistics were used to report findings. Results: Of 493 products identified, 231 products were analyzed. Two (0.9%) products, both of which were liquid formulations, contained at least 2,000 mg of EPA and DHA in the standard serving size listed on the labeling. The total amount of EPA and DHA per serving ranged from 60.2 mg to 2684 mg with an average of 697 mg. The number of servings necessary to achieve 2,000 mg of EPA and DHA ranged from 1 to 34 servings with an average of 5 servings. Conclusions: Serving sizes of fish oil products rarely result in adequate EPA and DHA intake to provide cholesterol-lowering benefit. Instruction by a trained healthcare professional, such as a pharmacist, is important to ensure patients are taking an appropriate serving of fish oil to obtain cardiovascular benefit.

scholarly journals Fish Oil Microencapsulation as Omega-3 Fatty Acids Fortification Material for Cream of Crab Soup

2015 ◽  
Vol 18 (2) ◽  
Author(s):  
Santiara Putri Pramestia ◽  
Bambang Riyanto ◽  
Wini Trilaksani
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Homogenization Treatment ◽  
Omega 3 Fatty Acid ◽  
Bioactive Component ◽  
Average Size ◽  
Serving Size

<p>Omega-3 fatty acids have important roles in improvement of intelligent and health<br />of human. Microencapsulation of fish oil as source of omega-3 fatty acids is an effort to<br />maintain flavor, aroma, stability, and also to successfully transfer bioactive component<br />from the fish oil as fortification material for foods or medicines. Improvement of instant<br />crab cream soup enriched with fish oil as source of omega-3 fatty acid has never been<br />conducted before. The purpose of this research was to improve microencapsulation<br />method for fish oil as source of omega-3 fatty acids as fortification material for instant<br />cream of crab soup. Microencapsulation methods in this research are homogenization<br />and spray drying. The results showed that the best microcapsule was obtained from<br />homogenization treatment for 10 minutes with efficiency of 90.41±0.64%. The shape of<br />the obtained microcapsule was spherical with average size of 6.52 μm, with induction time<br />up to 26.09±0.01 hours. The best cream of crab soup formula was at fish oil microcapsule </p><p>concentration of 3.30%, with 8.19% daily value of omega-3, inclusion 11.32% of EPA and<br />DHA at serving size of 17.56 gram.<br />Keywords : Crab, fish oil, fortification, instant cream soup, microencapsulation, omega-3<br />fatty acids</p>

scholarly journals Extraction of Omega-3 Fatty Acid from Jade Perch (Scortum barcoo) Using Enzymatic Hydrolysis Technique

2020 ◽  
Vol 20 (2) ◽  
pp. 282
Author(s):  
Nur Izzati Iberahim ◽  
Yee Chee Hann ◽  
Zainab Hamzah ◽  
Khairunissa Syairah Ahmad Sohaimi
Keyword(s):  
Fatty Acid ◽  
Enzymatic Hydrolysis ◽  
Fish Oil ◽  
Peroxide Value ◽  
Large Scale ◽  
Fatty Acid Content ◽  
Candida Rugosa ◽  
Scale Production ◽  
Omega 3 ◽  
Omega 3 Fatty Acid

Extraction of omega-3 fatty acid from Jade Perch (Scortum barcoo) using enzymatic hydrolysis techniques are expected to be more economically possible techniques due to the uses of the enzyme with the characteristic of environmentally friendly, reusable and less energy required during large-scale production. Design of Experiments (DOE) was used to study the effect of process parameters such as the concentration of alcalase (0.5–1.5%), temperature (50–70 °C) and pH (6.5–8.5) towards the yield of oil. The findings showed 16.55% of oil yield was extracted from the jade perch under an optimum condition at 50 °C, 6.5 pH with 0.5% of enzyme concentration for 2 h incubation time. The fish oil was then undergone enzymatic concentration of omega-3 FA using lipase from Candida rugosa. The acid value and peroxide value of the fish oil was 71.422 mg KOH/g and 0.799 meq/kg, while the acid and peroxide value of the omega-3 concentrated oil was lower to 49.074 mg KOH/g and 0.399 meq/kg. The FTIR spectrum showed the presence of C–H stretch, =C–H stretch and C=O stretch bond justified the existence of lipids as it presents of alkanes, alkene, and carboxylic acids functional group. At the same time, GC-MS analysis showed the fish oil contains higher total PUFA content and omega-3 fatty acid content than omega-3 concentrated oil.

scholarly journals Δ 6-desaturase activity in liver microsomes of rats fed diets enriched with cholesterol and/or ω3 fatty acids

1988 ◽  
Vol 249 (2) ◽  
pp. 351-356 ◽  
Author(s):  
M L Garg ◽  
E Sebokova ◽  
A B R Thomson ◽  
M T Clandinin
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Beef Tallow ◽  
Acid Content ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Omega 3 ◽  
Omega 3 Fatty Acid ◽  
Omega 6 ◽  
3 Omega

The effect of feeding semipurified diets enriched in linseed (rich in C18:3, omega 3 fatty acid) or fish (rich in C20:5, omega 3 and C22:6, omega 3 fatty acid) oil with and without cholesterol supplementation on the desaturation of linoleic acid (C18:2, omega 6) by rat liver microsomal fractions was investigated. Animals fed diets supplemented with beef tallow were used as equal-energy controls. Both linseed-oil and fish-oil diets, without added cholesterol, decrease conversion of C18:2, omega 6 fatty acid to gamma-linolenic acid (C18:3, omega 6). Reduction in delta 6-desaturation was significantly greater for animals fed the diet containing fish oil than with animals fed the linseed-oil diet. The major effect of cholesterol supplementation was to decrease the rate of desaturation of C18:2, omega 6, when fed in combination with the beef-tallow diet, whereas delta 6-desaturation was unaffected when cholesterol was fed along with diets high in omega 3 fatty acids (linseed oil or fish oil). The activity of the delta 6-desaturase in vitro is consistent with the fatty acid composition observed for the microsomal membranes on which this enzyme is localized. Dietary linseed oil and fish oil lowered the arachidonic (C20:4, omega 6) acid content of rat liver microsomes, with an accompanying increase in membrane eicosapentaenoic (C20:5, omega 3) and docosahexaenoic (C22:6, omega 3) acid content, in comparison with the group fed beef tallow. Inclusion of cholesterol into the beef-tallow or linseed-oil diets resulted in decreased membrane C20:4, omega 6-fatty-acid content, with concomitant increase in C18:2, omega 6-fatty-acid content. However, addition of cholesterol to the fish-oil diet did not alter the microsomal membrane content of C20:4, omega 6 fatty acid. Thus it is suggested that (1) the decrease in prostaglandin E2, thromboxane and prostacyclin levels generally observed after fish-oil consumption may be at least partly due to inhibition of C20:4, omega 6-fatty-acid synthesis from C18:2, omega 6 fatty acid; and (2) consumption of fish oil prevents the further decrease in C20:4, omega 6-fatty-acid levels by dietary cholesterol that is apparent when cholesterol is fed in combination with diets high in saturated fat or C18:3, omega 3 fatty acid.

scholarly journals Analysis of omega-3 fatty acid content of South African fish oil supplements

2011 ◽  
Vol 22 (6) ◽  
pp. 324-329 ◽  
Author(s):  
Maretha Opperman ◽  
De Wet Marais ◽  
A.J. Spinnler Benade
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
South African ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Omega 3 ◽  
Omega 3 Fatty Acid

scholarly journals Establishment of a Patient-Derived Xenograft Model of Colorectal Cancer in CIEA NOG Mice and Exploring Smartfish Liquid Diet as a Source of Omega-3 Fatty Acids

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 282
Author(s):  
Helle Samdal ◽  
Lene C Olsen ◽  
Knut S Grøn ◽  
Elin S Røyset ◽  
Therese S Høiem ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Content ◽  
Xenograft Model ◽  
Liquid Diet ◽  
Lymphoid Cells ◽  
Omega 3 ◽  
Pdx Model ◽  
Nog Mice

Cancer patient-derived xenografts (PDXs) better preserve tumor characteristics and microenvironment than traditional cancer cell line derived xenografts and are becoming a valuable model in translational cancer research and personalized medicine. We have established a PDX model for colorectal cancer (CRC) in CIEA NOG mice with a 50% engraftment rate. Tumor fragments from patients with CRC (n = 5) were engrafted in four mice per tumor (n = 20). Mice with established PDXs received a liquid diet enriched with fish oil or placebo, and fatty acid profiling was performed to measure fatty acid content in whole blood. Moreover, a biobank consisting of tissue and blood samples from patients was established. Histology, immunohistochemistry and in situ hybridization procedures were used for staining of tumor and xenograft tissue slides. Results demonstrate that key histological characteristics of the patients’ tumors were retained in the established PDXs, and the liquid diets were consumed as intended by the mice. Some of the older mice developed lymphomas that originated from human Ki67+, CD45+, and EBV+ lymphoid cells. We present a detailed description of the process and methodology, as well as possible issues that may arise, to refine the method and improve PDX engraftment rate for future studies. The established PDX model for CRC can be used for exploring different cancer treatment regimes, and liquid diets enriched with fish oil may be successfully delivered to the mice through the drinking flasks.

scholarly journals Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species

2021 ◽  
Vol 105 (5) ◽  
pp. 2139-2156
Author(s):  
Justine Sauvage ◽  
Gary H. Wikfors ◽  
Xiaoxu Li ◽  
Mark Gluis ◽  
Nancy Nevejan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Culture Media ◽  
Fatty Acid Content ◽  
Pluronic F127 ◽  
Algal Culture ◽  
Omega 3 ◽  
Chaetoceros Muelleri ◽  
Omega 3 Fatty Acid ◽  
Live Feed ◽  
Chaetoceros Calcitrans

Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros calcitrans, Chaetoceros muelleri, Skeletonema costatum, and Thalassiosira pseudonana; two haptophytes-Tisochrysis lutea and Pavlova salina). Algal culture performance in response to the addition of NAC and pluronic, singly or combined, is dosage- and species-dependent. Combined NAC and pluronic F127 algal culture media additives resulted in specific growth rate increases of 38%, 16%, and 24% for C. calcitrans, C. muelleri, and P. salina, respectively. Enhanced culture productivity for strains belonging to the genus Chaetoceros was paired with an ~27% increase in stationary-phase cell density. For some of the species examined, culture media enrichments with NAC and pluronic resulted in increased omega-3-fatty acid content of the algal biomass. Larval development (i.e., growth and survival) of the Pacific oyster (Crassostrea gigas) was not changed when fed a mixture of microalgae grown in NAC- and F127-supplemented culture medium. Based upon these results, we propose that culture media enrichment with NAC and pluronic F127 is an effective and easily adopted approach to increase algal productivity and enhance the nutritional quality of marine microalgal strains commonly cultured for live-feed applications in aquaculture. Key points • Single and combined NAC and pluronic F127 culture media supplementation significantly enhanced the productivity of Chaetoceros calcitrans and Chaetoceros muelleri cultures. • Culture media enrichments with NAC and F127 can increase omega-3-fatty acid content of algal biomass. • Microalgae grown in NAC- and pluronic F127-supplemented culture media are suitable for live-feed applications.

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