Evaluation of biological activity of bone morphogenetic proteins on exposure to commonly used electrospinning solvents

2011 ◽  
Vol 26 (6) ◽  
pp. 578-589 ◽  
Author(s):  
Parthasarathy A Madurantakam ◽  
Isaac A Rodriguez ◽  
Matthew J Beckman ◽  
David G Simpson ◽  
Gary L Bowlin
Keyword(s):  
Tissue Engineering ◽  
Biological Activity ◽  
Organic Solvent ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organic Solvents ◽  
Bone Morphogenetic Proteins ◽  
Biologically Active ◽  
Luciferase Reporter ◽  
Specific Response

Bone tissue engineering is one of the emerging strategies for developing functionally viable bone substitutes. The recent trend in bone tissue engineering is to combine the benefits of a three-dimensional nanofibrous scaffold with biologically active molecules and responsive stem cells. Electrospinning is the most versatile of the scaffold fabrication strategies and may involve the use of an organic solvent at one stage or another. In spite of all distinct advantages of electrospinning, valid concerns about potentially denaturing interactions between the organic solvent and the biomolecules exist. Efforts are ongoing to incorporate osteoinductive molecules, such as bone morphogenetic proteins (BMPs), during the electrospinning process. The challenge lies in ensuring that the biological activity of these incorporated molecules survives the process. This study was specifically designed to investigate the effects of exposure to commonly used organic solvents on heterodimeric BMP-2/7 using slot-blot assay quantified by infrared imaging and on embryonic myoblasts stably transfected with BMP-specific response element linked to a luciferase reporter – C2C12BRA. Overall, the biological activity of these molecules significantly decreased when exposed to organic solvents but can be restored to their original values by increasing the polarity of the solvent. It was found that an aqueous buffer can effectively overcome the deleterious effects of organic solvents on BMPs, thus generating osteoinductive bone scaffolds.

Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering

2015 ◽  
Vol 134 ◽  
pp. 799-807 ◽  
Author(s):  
Subramaniam Puvaneswary ◽  
Sepehr Talebian ◽  
Hanumantharao Balaji Raghavendran ◽  
Malliga Raman Murali ◽  
Mehdi Mehrali ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biological Activity ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Preparation of poly(l-lactic acid)/β-tricalcium phosphate scaffold for bone tissue engineering without organic solvent

2008 ◽  
Vol 62 (12-13) ◽  
pp. 2029-2032 ◽  
Author(s):  
Yunqing Kang ◽  
Guangfu Yin ◽  
Quan Yuan ◽  
Yadong Yao ◽  
Zhongbing Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Organic Solvent ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tricalcium Phosphate

scholarly journals Current Trends in Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Marco Mravic ◽  
Bruno Péault ◽  
Aaron W. James
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Biology ◽  
Wnt Signaling Pathway ◽  
Biologically Active ◽  
Translational Medical Research ◽  
Computer Assisted ◽  
Current Trends

The development of tissue engineering and regeneration constitutes a new platform for translational medical research. Effective therapies for bone engineering typically employ the coordinated manipulation of cells, biologically active signaling molecules, and biomimetic, biodegradable scaffolds. Bone tissue engineering has become increasingly dependent on the merging of innovations from each of these fields, as they continue to evolve independently. This foreword will highlight some of the most recent advances in bone tissue engineering and regeneration, emphasizing the interconnected fields of stem cell biology, cell signaling biology, and biomaterial research. These include, for example, novel methods for mesenchymal stem cell purification, new methods of Wnt signaling pathway manipulation, and cutting edge computer assisted nanoscale design of bone scaffold materials. In the following special issue, we sought to incorporate these diverse areas of emphasis in order to reflect current trends in the field.

scholarly journals The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications

2021 ◽  
Vol 22 (6) ◽  
pp. 3086
Author(s):  
Stefanos Kikionis ◽  
Efstathia Ioannou ◽  
Eleni Aggelidou ◽  
Leto-Aikaterini Tziveleka ◽  
Efterpi Demiri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Hybrid Composites ◽  
Sulfated Polysaccharide ◽  
Biologically Active ◽  
Bone Tissue Regeneration ◽  
Natural Polymers ◽  
The Impact

Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering. Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties. Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized. The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells. The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.

scholarly journals Calcium Orthophosphate (CaPO4) Scaffolds for Bone Tissue Engineering Applications

2018 ◽  
Vol 1 (3) ◽  
pp. 25-93 ◽  
Author(s):  
Sergey V. Dorozhkin
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Bone Grafts ◽  
Biologically Active ◽  
Growth Factor Delivery ◽  
Engineering Applications ◽  
Scaffold Materials

The chemical and structural similarities of calcium orthophosphates (abbreviated as CaPO4)to the mineral composition of natural bones and teeth have made them a good candidate for bone tissue engineering applications. Nowadays, a variety of natural or synthetic CaPO4-based biomaterials is produced and has been extensively used for dental and orthopedic applications. Despite their inherent brittleness, CaPO4 materials possess several appealing characteristics as scaffold materials. Namely, their biocompatibility and variable stoichiometry, thus surface charge density, functionality and dissolution properties, make them suitable for both drug and growth factor delivery. Therefore, CaPO4, especially hydroxyapatite (HA) and tricalcium phosphates (TCPs), have attracted a significant interest in simultaneous use as bone grafts and drug delivery vehicles. Namely, CaPO4-based three-dimensional (3D) scaffolds and/or carriers have been designed to induce bone formation and vascularization. These scaffolds are usually porous and harbor various types of drugs, biologically active molecules and/or cells. Over the past few decades, their application as bone grafts in combination with stem cells has gained much importance. This review discusses the source, manufacturing methods and advantages of using CaPO4 scaffolds for bone tissue engineering applications. Perspective future applications comprise drug delivery and tissue engineering purposes.

Eggshell particle-reinforced hydrogels for bone tissue engineering: an orthogonal approach

2019 ◽  
Vol 7 (7) ◽  
pp. 2675-2685 ◽  
Author(s):  
Xinchen Wu ◽  
Stephanie I. Stroll ◽  
Darlin Lantigua ◽  
Sanika Suvarnapathaki ◽  
Gulden Camci-Unal
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biologically Active

Eggshell microparticle-reinforced hydrogels have been fabricated and characterized to obtain mechanically stable and biologically active scaffolds that can direct the differentiation of cells.

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