scholarly journals Amniotic membrane allografts maintain key biological properties post SCCO2 and lyophilization processing

2020 ◽  
Vol 35 (6) ◽  
pp. 592-601
Author(s):  
Jennifer S McDaniel ◽  
Jennifer L Wehmeyer ◽  
Lauren E Cornell ◽  
Anthony J Johnson ◽  
David O Zamora
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Biological Tissues ◽  
Storage Conditions ◽  
Biological Properties ◽  
Biochemical Properties ◽  
Specific Storage ◽  
Ecm Proteins ◽  
Corneal Wound ◽  
Processing Techniques

Amniotic membrane (AM) has been shown to enhance corneal wound healing due to the abundance of growth factors, cytokines, and extracellular matrix (ECM) proteins inherent to the tissue. As such, AM has garnered widespread clinical utility as a biological dressing for a number of ophthalmic and soft tissue applications. The preparation, sterilization, and storage procedures used to manufacture AM grafts are extremely important for the conservation of inherent biological components within the membrane. Current processing techniques use harsh chemicals and sterilization agents that can compromise the fundamental wound healing properties of AM. Furthermore, commercially available cryopreserved AM products require specific storage conditions (e.g., ultra-low freezers) thereby limiting their clinical availability in austere environments. Supercritical carbon dioxide (SCCO2) technology allows for the sterilization of biological tissues without the resulting degradation of integral ECM proteins and other factors often seen with current tissue sterilization processes. With this study we demonstrate that lyophilized AM, sterilized using SCCO2, maintains similar biochemical properties and biocompatibility as that of commercially available AM products requiring specialized cold storage conditions.

Amniotic membrane transplantation (AMT): Implications for corneal wound healing

2008 ◽  
Vol 86 ◽  
pp. 0-0
Author(s):  
DG SAID ◽  
M NUBILE ◽  
A HOPKINSON ◽  
T GRAY ◽  
J LOWE ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Corneal Wound Healing ◽  
Corneal Wound

Histologic Features of Transplanted Amniotic Membrane: Implications for Corneal Wound Healing

Ophthalmology ◽  
2009 ◽  
Vol 116 (7) ◽  
pp. 1287-1295 ◽  
Author(s):  
Dalia G. Said ◽  
Mario Nubile ◽  
Thaer Alomar ◽  
Andy Hopkinson ◽  
Trevor Gray ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Corneal Wound Healing ◽  
Corneal Wound

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

scholarly journals Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin Zhou ◽  
Ludvig J. Backman ◽  
Patrik Danielson
Keyword(s):  
Wound Healing ◽  
Mitochondrial Dna ◽  
Scar Formation ◽  
Mitochondrial Rna ◽  
Peripheral Part ◽  
Double Stranded Rna ◽  
Corneal Wound ◽  
Biochemical Approach ◽  
Common Deletion ◽  
Underlying Mechanisms

AbstractScar formation as a result of corneal wound healing is a leading cause of blindness. It is a challenge to understand why scar formation is more likely to occur in the central part of the cornea as compared to the peripheral part. The purpose of this study was to unravel the underlying mechanisms. We applied RNA-seq to uncover the differences of expression profile in keratocytes in the central/peripheral part of the cornea. The relative quantity of mitochondrial RNA was measured by multiplex qPCR. The characterization of mitochondrial RNA in the cytoplasm was confirmed by immunofluoresence microscope and biochemical approach. Gene expression was analyzed by western blot and RT qPCR. We demonstrate that the occurrence of mitochondrial DNA common deletion is greater in keratocytes from the central cornea as compared to those of the peripheral part. The keratocytes with CD have elevated oxidative stress levels, which leads to the leakage of mitochondrial double-stranded RNA into the cytoplasm. The cytoplasmic mitochondrial double-stranded RNA is sensed by MDA5, which induces NF-κB activation. The NF-κB activation thereafter induces fibrosis-like extracellular matrix expressions and IL-8 mRNA transcription. These results provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

scholarly journals Development of Spanish Broom and Flax Dressings with Glycyrrhetinic Acid-Loaded Films for Wound Healing: Characterization and Evaluation of Biological Properties

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1192
Author(s):  
Angela Abruzzo ◽  
Concettina Cappadone ◽  
Valentina Sallustio ◽  
Giovanna Picone ◽  
Martina Rossi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Drug Loading ◽  
Healing Process ◽  
In Vitro Release ◽  
Sodium Hyaluronate ◽  
Biological Properties ◽  
Glycyrrhetinic Acid ◽  
Wound Dressings ◽  
Biological Studies

The selection of an appropriate dressing for each type of wound is a very important procedure for a faster and more accurate healing process. So, the aim of this study was to develop innovative Spanish Broom and flax wound dressings, as alternatives to cotton used as control, with polymeric films containing glycyrrhetinic acid (GA) to promote wound-exudate absorption and the healing process. The different wound dressings were prepared by a solvent casting method, and characterized in terms of drug loading, water uptake, and in vitro release. Moreover, biological studies were performed to evaluate their biocompatibility and wound-healing efficacy. Comparing the developed wound dressings, Spanish Broom dressings with GA-loaded sodium hyaluronate film had the best functional properties, in terms of hydration ability and GA release. Moreover, they showed a good biocompatibility, determining a moderate induction of cell proliferation and no cytotoxicity. In addition, the wound-healing test revealed that the Spanish Broom dressings promoted cell migration, further facilitating the closure of the wound.

Corneal Wound Healing in Vitro

1963 ◽  
Vol 56 (5) ◽  
pp. 833-834
Author(s):  
N.A. Wine ◽  
P.K. Basu
Keyword(s):  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound
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