An Association of Mercury with Selenium in Inorganic Mercury Intoxication

1 Energy dispersive X-ray analysis was performed on the renal tubular cells of two patients with inorganic mercury intoxication. 2 Some lysosomes of these cells consisted of unusual matrices of aggregated electron-dense grains which were positive for mercury, selenium and sulphur. 3 Though maps of the specific X-rays of both mercury and selenium coincided exactly with these lysosomes, the molecular ratio of selenium to mercury ranged between zero and 2.9. 4 It is unlikely that the trace element of selenium and exogenous inorganic mercury are deposited in the lysosomes independent of each other, but rather their coexistence in the characteristic lysosomes strongly suggests a compound formed by binding mercury to the SeH residues of selenoprotein.

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Bismuth localization within nuclear inclusions by x-ray microanalysis. Effects of accelerating voltage.

Journal of Histochemistry & Cytochemistry ◽

10.1177/23.10.1194662 ◽

1975 ◽

Vol 23 (10) ◽

pp. 722-726 ◽

Cited By ~ 33

Author(s):

B A Fowler ◽

R A Goyer

Keyword(s):

Inclusion Bodies ◽

Energy Dispersive ◽

Nuclear Inclusions ◽

X Rays ◽

Nuclear Inclusion ◽

X Ray ◽

Renal Tubular ◽

Lower Background ◽

Analytical Detection

This report details the localization of bismuth by energy-dispersive x-ray microanalysis within characteristic nuclear inclusion bodies of renal tubular lining cells of rats following excessive exposure to this element. Peak to background ratios and analytical detection sensitivities for bismuth were found to vary for 04, 60 or 80 keV electron accelerating voltages. Optimum peak to background ratios were observed at 40 keV due to lower background generation, while greater detection sensitivities were recorded at 80 keV due to enhanced generation of bismuth characteristic x-rays.

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PHAX-SCAN: Functional integration of a Scanning Electron Microscope and an energy-dispersive x-ray analyser

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100152252 ◽

1989 ◽

Vol 47 ◽

pp. 56-57

Author(s):

Marc H. Peeters ◽

Max T. Otten

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

High Speed ◽

High Performance ◽

Functional Integration ◽

Energy Dispersive ◽

X Rays ◽

X Ray ◽

High Speed Analysis ◽

Scanning Electron

Over the past decades, the combination of energy-dispersive analysis of X-rays and scanning electron microscopy has proved to be a powerful tool for fast and reliable elemental characterization of a large variety of specimens. The technique has evolved rapidly from a purely qualitative characterization method to a reliable quantitative way of analysis. In the last 5 years, an increasing need for automation is observed, whereby energy-dispersive analysers control the beam and stage movement of the scanning electron microscope in order to collect digital X-ray images and perform unattended point analysis over multiple locations.The Philips High-speed Analysis of X-rays system (PHAX-Scan) makes use of the high performance dual-processor structure of the EDAX PV9900 analyser and the databus structure of the Philips series 500 scanning electron microscope to provide a highly automated, user-friendly and extremely fast microanalysis system. The software that runs on the hardware described above was specifically designed to provide the ultimate attainable speed on the system.

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Comparison of high-angle take-off and low-angle take-off EDX detector geometry of the HF-2000 FE-TEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147107 ◽

1993 ◽

Vol 51 ◽

pp. 252-253

Author(s):

Y. Sato ◽

T. Hashimoto ◽

M. Ichihashi ◽

Y. Ueki ◽

K. Hirose ◽

...

Keyword(s):

Quantitative Analysis ◽

Field Emission ◽

Solid Angle ◽

Energy Dispersive ◽

Objective Lens ◽

X Rays ◽

High Angle ◽

X Ray ◽

Detector Geometry

Analytical TEMs have two variations in x-ray detector geometry, high and low angle take off. The high take off angle is advantageous for accuracy of quantitative analysis, because the x rays are less absorbed when they go through the sample. The low take off angle geometry enables better sensitivity because of larger detector solid angle.Hitachi HF-2000 cold field emission TEM has two versions; high angle take off and low angle take off. The former allows an energy dispersive x-ray detector above the objective lens. The latter allows the detector beside the objective lens. The x-ray take off angle is 68° for the high take off angle with the specimen held at right angles to the beam, and 22° for the low angle take off. The solid angle is 0.037 sr for the high angle take off, and 0.12 sr for the low angle take off, using a 30 mm2 detector.

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