A protein disulfide-thiol interchange protein with NADH: protein disulfide reductase (NADH oxidase) activity as a molecular target for low levels of exposure to organic solvents in plant growth

1998 ◽  
Vol 17 (5) ◽  
pp. 272-277 ◽  
Author(s):  
D J Morré
Keyword(s):  
Plant Growth ◽  
Nadh Oxidase ◽  
Biological Effects ◽  
Membrane Vesicles ◽  
Molecular Target ◽  
Plasma Membrane Vesicles ◽  
Plant Parts ◽  
Disulfide Reductase ◽  
Low Levels ◽  
Protein Disulfide

A number of solvents including ethyl, amyl, butyl, octyl and benzyl alcohols, ethylene glycol, ethyl acetate, acetone, diethyl ether, propylene oxide, r-dioxane, benzene, xylene, chloroform and carbon tetrachloride stimulate the growth of plants or plant parts at low concentrations and inhibit at high concentrations. These same solvents, at low dilutions, stimulate the activity of a growth-related protein disulfide-thiol interchange protein (TIP) with NADH: protein disulfide reductase (NADH oxidase) (NOX) activity with plasma membrane vesicles isolated from elongating regions cut from dark grown seedlings of soybeans. Based on these and other findings, we suggest the TIP/NOX protein to be the molecular target of the biological effects of low levels of exposure (hormesis) involved in the stimulation of plant growth.

Plastic Mulch Color Effects on Light Micro-environment and Watermelon Plant Growth

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 474d-474
Author(s):  
N.K. Damayanthi Ranwala ◽  
Dennis R. Decoteau
Keyword(s):  
Plant Growth ◽  
Light Transmission ◽  
Root Zone ◽  
Dry Mass ◽  
Plant Responses ◽  
Plastic Mulch ◽  
Plant Parts ◽  
Reflected Light ◽  
Total Light ◽  
Dry Mass Partitioning

This study was conducted to evaluate the spectral properties of various colored plastic color mulches and to determine the effects of upwardly reflected light from the mulch surfaces on watermelon plant growth when differences in root zone temperatures are minimized. Two-week-old watermelon plants were grown with black mulch, red-painted mulch, SRM-Red mulch (Sonoco, Inc., Harstville, S.C.), and white mulch. Total light reflection (58 μmol·m–2·s–1 in 400–700 nm) and red: far-red (R:FR = 0.44) of reflected light were lower in black mulch and highest in white mulch (634 and 0.92, respectively). Both black mulch and white mulch had same blue:red (B:R = 0.6) while white mulch had higher B:FR (0.58) in reflected light compared to black mulch (0.26). Reflective properties of red mulches were somewhat similar, and R:FR, B:R, and B:FR were 0.8, 0.2, and 0.18, respectively. However, SRM-Red mulch had highest total light (355 μmol·m–2·s–1 in 400–700 nm) transmission through the mulch, and R:FR, B:R, and B:FR were 0.84, 0.28, and 0.23, respectively. Light transmission through the other mulches was nonsignificant. Watermelon plants grown with black mulch and red mulches had higher internode lengths compared to white mulch after 20 days. Further, plants grown under black had significant higher petiole elongation accompanied with higher dry mass partitioning to petioles, and lower partitioning to roots, stems, and leaves. There was no effects of surface mulch color on total plant dry mass or photosynthesis although plants with black had higher transpiration rate. This suggests the differential regulation of dry mass partitioning among plant parts due to mulch color. The similar plant responses with black mulch and white mulch to plants treated with FR or R light at the end of photoperiod implies the involvement of phytochrome regulation of growth due to mulch surface color.

scholarly journals Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreia I. Pimenta ◽  
Nuno Bernardes ◽  
Marta M. Alves ◽  
Dalila Mil-Homens ◽  
Arsenio M. Fialho
Keyword(s):  
Membrane Vesicles ◽  
Bronchial Epithelial Cell ◽  
Host Cells ◽  
Burkholderia Cenocepacia ◽  
Transport Systems ◽  
Epithelial Cell Line ◽  
Plasma Membrane Vesicles ◽  
Bronchial Epithelial ◽  
Cellular Processes ◽  
Associated Functions

AbstractBurkholderia cenocepacia is known for its capacity of adherence and interaction with the host, causing severe opportunistic lung infections in cystic fibrosis patients. In this work we produced Giant Plasma Membrane Vesicles (GPMVs) from a bronchial epithelial cell line and validated their use as a cell-like alternative to investigate the steps involved in the adhesion process of B. cenocepacia. RNA-sequencing was performed and the analysis of the B. cenocepacia K56-2 transcriptome after the first contacts with the surface of host cells allowed the recognition of genes implicated in bacterial adaptation and virulence-associated functions. The sensing of host membranes led to a transcriptional shift that caused a cascade of metabolic and physiological adaptations to the host specific environment. Many of the differentially expressed genes encode proteins related with central metabolic pathways, transport systems, cellular processes, and virulence traits. The understanding of the changes in gene expression that occur in the early steps of infection can uncover new proteins implicated in B. cenocepacia-host cell adhesion, against which new blocking agents could be designed to control the progression of the infectious process.

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