scholarly journals Solitary Dermal Leiomyosarcomas in 12 Ferrets

2002 ◽  
Vol 14 (3) ◽  
pp. 262-265 ◽  
Author(s):  
Igor Mikaelian ◽  
Michael M. Garner
Keyword(s):  
Smooth Muscle ◽  
High Power ◽  
Smooth Muscle Actin ◽  
Muscle Fibers ◽  
Mitotic Rate ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
Adrenal Disease

Twelve 3–6-year-old ferrets (8 males, 3 females, 1 unknown) were presented with single cutaneous nodules. These dermal tumors were characterized histologically by nodular proliferation of neoplastic smooth muscle fibers with marked anisokaryosis and a mitotic rate of >2 mitoses per 10 high-power fields. Neoplastic cells stained strongly for vimentin in all tumors and for smooth muscle actin and desmin in all but 1 tumor. Histologic and immunohistochemical findings suggested a diagnosis of piloleiomyosarcoma for these tumors. Excision was curative in all animals available for follow-up. However, 3 of 5 animals developed adrenal disease within 7 months after removal of the dermal leiomyosarcoma.

Piloleiomyosarcoma in Seven Ferrets

2001 ◽  
Vol 38 (6) ◽  
pp. 710-711 ◽  
Author(s):  
B. H. Rickman ◽  
L. E. Craig ◽  
M. H. Goldschmidt
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Immunohistochemical Staining ◽  
Smooth Muscle Actin ◽  
Surgical Excision ◽  
Muscle Actin ◽  
Mustela Putorius ◽  
Nuclear Pleomorphism ◽  
Complete Surgical Excision

In each of seven ferrets ( Mustela putorius furo) with leiomyosarcoma, a single dermal mass was identified and biopsied. Each mass consisted of a well-demarcated but nonencapsulated proliferation of large spindle- to strap-shaped cells arranged in interwoven bundles. The cells resembled the smooth muscle cells of the adjacent arrector pili muscles, but with marked nuclear pleomorphism. Immunohistochemical staining for smooth muscle actin, desmin, and vimentin was positive and staining for myoglobin and cytokeratin was negative. Follow-up on three of the ferrets indicates that the prognosis is good following complete surgical excision.

Pulmonary Myxoma in a Sheep

2009 ◽  
Vol 46 (3) ◽  
pp. 457-459 ◽  
Author(s):  
F. Ilhan ◽  
Z. Yener
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Left Lung ◽  
Stellate Cells ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
S 100 ◽  
Myxoid Matrix ◽  
Uncommon Neoplasm

Pulmonary myxoma is an uncommon neoplasm. A pale tan, lobulated, and well-circumscribed mass was discovered at slaughter in the left lung of a 5-year-old sheep. Histologically, the tumor was composed of spindloid to stellate cells in a myxoid matrix. Neoplastic cells were immunohistochemically positive for vimentin but did not express cytokeratins, S-100 protein, smooth muscle actin, desmin, or p53. On the basis of the histologic and immunohistochemical findings, this tumor was diagnosed as a myxoma.

scholarly journals Immunophenotype Heterogeneity in Nasal Glomangiopericytoma

2015 ◽  
Vol 2015 ◽  
pp. 1-3 ◽  
Author(s):  
Adriana Handra-Luca ◽  
Zakaria Y. Abd Elmageed ◽  
Christina Magkou ◽  
Marick Lae
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Expression Patterns ◽  
Resected Specimen ◽  
Muscle Actin ◽  
Vascular Cell ◽  
Drug Treatments ◽  
Related Proteins ◽  
Complex Surgery

Nasal glomangiopericytoma is rare. The immunophenotype is heterogeneous, more frequently smooth-muscle-actin and CD34-positive. We report expression patterns for several vascular-related proteins such as CD99, CD146, Bcl2, and WT1 as well as for treatment-related proteins such as mTOR and EGFR in a nasal glomangiopericytoma. The patient (woman, 86 years) presented with a left nasal tumefaction. The resected specimen (1.5-cm) showed a glomangiopericytoma. Tumor cells expressed smooth-muscle-actin, CD31, CD34, and progesterone receptor. They also expressed the vascular-cell-related proteins Bcl2, CD99, CD146, and WT1, as well as mTOR and EGFR. Nasal glomangiopericytomas show immunohistochemical heterogeneity for vascular-related markers, suggesting a possible extensive pericytic differentiation. The expression of potential targets for drug treatments such as mTOR and EGFR may impact on the clinical follow-up of these tumors occurring at advanced ages, which may require complex surgery.

Malignant Solitary Fibrous Tumor of the Pleura With Liposarcomatous Differentiation

2001 ◽  
Vol 125 (3) ◽  
pp. 406-409
Author(s):  
Hongwei Bai ◽  
Bassam I. Aswad ◽  
Henning Gaissert ◽  
Douglas R. Gnepp
Keyword(s):  
Smooth Muscle ◽  
Tumor Cells ◽  
Solitary Fibrous Tumor ◽  
Smooth Muscle Actin ◽  
Mitotic Rate ◽  
Muscle Actin ◽  
Solitary Fibrous Tumors ◽  
Collagenous Stroma ◽  
Well Differentiated ◽  
Fibrous Tumor

Abstract Pleural solitary fibrous tumors (SFTs) are uncommon tumors. Although these tumors have been well characterized, malignant pleural SFTs with liposarcomatous differentiation have not been reported. We report an unusual malignant pleural SFT intermixed with foci of well-differentiated liposarcoma. The patient was a 66-year-old, white man with a large, solid right pleural mass that measured 13.5 × 10.3 × 8.5 cm. The tumor was composed of spindle-shaped and plump cells embedded in dense collagenous stroma. The tumor cells were arranged in interlacing fascicles or in a patternless pattern. Marked nuclear atypia, a high mitotic rate (21 mitoses per 10 high-power fields), and areas of prominent necrosis were evident. In addition, numerous adipocytes mixed with typical lipoblasts were seen scattered throughout portions of the tumor. Immunohistochemistry revealed the tumor cells were strongly positive for CD34 and vimentin and negative for cytokeratin, desmin, smooth muscle actin (IA4), and S100. To the best of our knowledge, this case represents the first example of a malignant SFT with liposarcomatous differentiation.

scholarly journals Piloleiomyosarcoma in cats: Histological and immunohistochemical features

2021 ◽  
pp. 030098582110425
Author(s):  
Francisco Rodríguez Guisado ◽  
Pedro Luis Castro
Keyword(s):  
Smooth Muscle ◽  
Subcutaneous Tissue ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Surgical Excision ◽  
Giant Cells ◽  
Hair Follicles ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
S 100

This study describes the histomorphology and immunohistochemical profile of 9 cases of feline piloleiomyosarcoma. Cats ranged in age from 7 to 16 years (mean 10), and tumors were 7 to 24 mm in diameter (mean 15). Tumors were composed of fusiform cells that were haphazardly arranged or in variably sized interwoven bundles. Neoplastic cells had eosinophilic and fibrillar cytoplasm, and elongated blunt-ended nuclei. Entrapment of hair follicles and absence of vascular components support an origin from the smooth muscle cells of the arrector pili. Additional findings included bizarre nuclei and giant cells (7/9 cases), atypical mitoses (7/9 cases), ulceration (3/9 cases), and intratumoral necrosis (6/9 cases). Neoplastic cells expressed calponin, desmin, α-smooth muscle actin, and vimentin, but not CD18, CD31, cytokeratins, glial fibrillary acidic protein, neuron-specific enolase, Melan A, p63, or S-100 protein. Surgical excision was curative in 6/9 cases, with local recurrence in 2/9 cases and metastasis to local lymph nodes in 1/9 case. Clinical outcome was influenced by mitotic count, infiltration of subcutaneous tissue, and intensity of nuclear immunolabeling for p53.

Prostatic Rhabdomyoma in a Toddler: A Case Report With Molecular Characterization

2021 ◽  
pp. 109352662110469
Author(s):  
Paola X. De la Iglesia Niveyro ◽  
J. Pandolfi ◽  
F. Jauk ◽  
T. Kreindel ◽  
P. Lobos
Keyword(s):  
Differential Diagnosis ◽  
Smooth Muscle ◽  
Case Report ◽  
Next Generation Sequencing ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Ultrasound Evaluation ◽  
Microscopic Evaluation ◽  
Generation Sequencing

We present a 29-month-old male patient in follow-up due to pyelocaliceal dilation with a prostatic nodule incidentally found during ultrasound evaluation. Cysto video endoscopy was performed and a prostate biopsy, obtained. Microscopic evaluation showed a haphazardly distributed population of muscular cells with cross striations without evidence of mitosis or necrosis. Immunohistochemistry was positive for myogenin and desmin and negative for smooth muscle actin. Next generation sequencing was performed without finding any pathogenic variant or fusion in the tumor RNA. The patient received no further treatment, remained asymptomatic and continues in follow up, 3 years after initial diagnosis. We report a case of prostate rhabdomyoma in a toddler, an exceptional location that raises concern about differential diagnosis with its malignant counterpart, rhabdomyosarcoma, especially at this age.

scholarly journals Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

2021 ◽  
Vol 11 (8) ◽  
pp. 3524
Author(s):  
Azeem Ul Yaqin Syed ◽  
Muhammad A. Ahmed ◽  
Eman I. AlSagob ◽  
Mansour Al-Askar ◽  
Abdulrahman M. AlMubarak ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Control Cell ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Muscle Actin ◽  
Catha Edulis ◽  
Squamous Carcinoma Cells ◽  
Dose Dependent

The aim was to determine the cytotoxicity of Khat (Catha edulis (Vahl) Forssk. ex Endl) on normal oral fibroblasts (NOFs) and SCC4 (squamous carcinoma cells) along with expression of α-smooth muscle actin (α-SMA) in fibroblasts. Khat filtrate was prepared to obtain a concentrated viscous solution. NOFs and SCC4 cells were cultured in biological cabinets and were grown in Dulbeccos’ modified Eagles medium. Frozen cells were thawed at 37 °C and cell seeding was performed. NOFs and SCC4 cells were seeded on 96 well plates and allowed to attach. The medium was removed and a fresh medium containing different concentrations of Khat was added. The group without Khat served as a negative control and 4% paraformaldehyde as the positive control. Cell viability was assessed using the MTT assay and effect of Khat on fibroblast and SCC4 phenotypes was evaluated by immunostaining. Analysis of variance was used to assess data (p < 0.05). NOF 316 showed cell death in response to 4% paraformaldehyde, 12.5, 6.25, and 3.12 mg/mL of Khat. The highest concentration of Khat (25 mg/mL) failed to cause cytotoxicity of NOF 316. NOF 319 and NOF 26 displayed cell death at all concentrations of Khat, however, cytotoxicity was not dose dependent. NOF 18 and SCC4 cells showed dose-dependent cell death. NOF 316 showed α-SMA expression after 1 mg/mL of Khat exposure. Not all fibroblasts were α-SMA-positive, suggesting specific activation of a subset of fibroblasts. Khat is cytotoxic to NOF and SCC4 cells. Furthermore, it can also cause activation and phenotypic changes in oral fibroblasts, indicating a potential role in progression of oral squamous cell carcinoma.

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