p53 Immunoexpression in Carcinomas Arising in Pleomorphic Adenoma

1996 ◽  
Vol 3 (4) ◽  
pp. 257-262 ◽  
Author(s):  
Joaninha Costa Rosa ◽  
Isabel Fonseca ◽  
Ana Félix ◽  
Jorge Soares
Keyword(s):  
Pleomorphic Adenoma ◽  
P53 Protein ◽  
P53 Gene ◽  
Tumor Grade ◽  
Undifferentiated Carcinoma ◽  
Protein Accumulation ◽  
Low Grade ◽  
Histologic Type ◽  
Epithelial Myoepithelial Carcinoma ◽  
Invasive Carcinomas

p53 protein immunoexpression was evaluated in 17 invasive carcinomas arising in pleomorphic adenoma and correlated with the histologic type and tumor grade. Ten tumors had one malignant histologic component: adenocarcinoma NOS (not otherwise specified) (five), undifferentiated carcinoma (two), malignant myoepithelioma (one), epithelial-myoepithelial carcinoma (one), and malignant oncocytoma (one). In the remaining cases, there was a coexistence of areas of adenocarcinoma (seven), adenosquamous (two), epithelial-myoepithelial (two), adenoid cystic (two), undifferentiated carcinoma (one), and low-grade polymorphous adenocarcinoma (one). p53 positivity was found within adenocarcinoma NOS (three) and adenosquamous carcinoma (two) components of four cases. Tumor areas showing low-grade histology, either mono- or bidifferentiated carcinomas, were always negative in this series, in keeping with previous observations on primary neoplasms of the same histologic type. The benign component of the neoplasms was also found to be consistently negative. The results point to a preferential association of the p53 gene dysfunction and its protein accumulation with the malignant transformation of pleomorphic adenomas into salivary adenocarcinomas with features of high-grade malignancy.

p53 protein accumulation and p53 gene mutation in colorectal cancer

2000 ◽  
Vol 6 (4) ◽  
pp. 275-279 ◽  
Author(s):  
Anna Nasierowska-Guttmejer ◽  
Lech Trzeciak ◽  
Marek P. Nowacki ◽  
Jerzy Ostrowski
Keyword(s):  
Colorectal Cancer ◽  
Gene Mutation ◽  
P53 Protein ◽  
P53 Gene ◽  
Protein Accumulation ◽  
P53 Gene Mutation

scholarly journals p53 protein accumulation and p53 gene mutation in esophageal carcinoma

Cancer ◽  
1997 ◽  
Vol 79 (3) ◽  
pp. 425-432 ◽  
Author(s):  
Guido Coggi ◽  
Silvano Bosari ◽  
Massimo Roncalli ◽  
Daniela Graziani ◽  
Paola Bossi ◽  
...  
Keyword(s):  
Esophageal Carcinoma ◽  
Gene Mutation ◽  
P53 Protein ◽  
P53 Gene ◽  
Protein Accumulation ◽  
P53 Gene Mutation

p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation

1995 ◽  
Vol 108 (4) ◽  
pp. 1176-1182 ◽  
Author(s):  
Jean-Christophe Bourdon ◽  
Antonia D'Errico ◽  
Patrizia Paterlini ◽  
Walter Grigioni ◽  
Evelyne May ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Gene Mutation ◽  
P53 Protein ◽  
P53 Gene ◽  
Protein Accumulation ◽  
P53 Gene Mutation

scholarly journals MDM2 overexpression does not account for stabilization of wild-type p53 protein in non-Hodgkin's lymphomas

Blood ◽  
1995 ◽  
Vol 85 (11) ◽  
pp. 3239-3246 ◽  
Author(s):  
R Maestro ◽  
A Gloghini ◽  
C Doglioni ◽  
D Gasparotto ◽  
T Vukosavljevic ◽  
...  
Keyword(s):  
P53 Protein ◽  
P53 Gene ◽  
Critical Ratio ◽  
Low Grade ◽  
High Grade ◽  
Protein Overexpression ◽  
Wild Type ◽  
Donor And Acceptor ◽  
Hodgkin's Lymphomas ◽  
Mdm2 Overexpression

p53 protein overexpression is a frequent finding in non-Hodgkin's lymphomas (NHL), being detected in over 25% of the cases. Moreover, some high-grade lymphomas and a large fraction of low-grade tumors show a pattern of scattered p53 accumulation in a limited percentage of neoplastic cells. In contrast, NHLs show a low frequency of p53 gene mutations. To investigate the molecular bases of p53 protein overexpression, a large series of NHLs was analyzed for p53 gene status. The analysis of the entire coding region of the gene (exons 2–11) and corresponding donor and acceptor splicing sites indicated that a significant proportion of p53-positive tumors overexpresses a wild-type form of p53 protein (wt-p53). To assess whether wt-p53 accumulation was related to the formation of inactive complexes with endogenous proteins, MDM2 oncogene expression and amplification were analyzed. MDM2 overexpression was detected only in one third of the wt-p53-positive cases, thus excluding that MDM2 accounts tout court for the accumulation of a normal p53 protein. However, the fact that MDM2 overexpression was detected in only the p53-positive cases and the observation that MDM2-positive cells were a subpopulation of p53-positive cells suggest a link between the two phenomena. In particular, our results indicate that the accumulation of a wt form of p53 protein could promote the overexpression of the MDM2 gene product. In addition, the prevalence of MDM2 positivity in intermediate/high-grade tumors together with the concordant expression of wt-p53 and MDM2 only in the high-grade component of a ‘composite’ lymphoma suggests that perturbation in the MDM2/p53 critical ratio could play a role in lymphoma progression.

scholarly journals BRAF Mutation Is Associated with Hyperplastic Polyp-Associated Gastric Cancer

2021 ◽  
Vol 22 (23) ◽  
pp. 12724
Author(s):  
Rina Fujiwara-Tani ◽  
Ayaka Okamoto ◽  
Hiroyuki Katsuragawa ◽  
Hitoshi Ohmori ◽  
Kiyomu Fujii ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Granulation Tissue ◽  
Braf Mutation ◽  
Large Scale ◽  
Hyperplastic Polyp ◽  
P53 Protein ◽  
Protein Accumulation ◽  
Low Grade ◽  
Mucin Phenotype ◽  
H Pylori

Gastric hyperplastic polyps (GHP) are frequently found to be benign polyps and have been considered to have a low carcinogenic potential. The characteristics of the hyperplastic polyp-associated gastric cancer (HPAGC) remain unclear. Therefore, we analyzed samples from 102 GHP patients and identified 20 low-grade atypical GHPs (19.6%), 7 high-grade atypical GHPs (6.9%), and 5 intramucosal cancer samples (4.9%). GHP atypia was more common in the elderly and increased with increasing polyp size. In particular, polyps larger than 1 cm were associated with a higher grade and cancer. Furthermore, mucus production decreased with increasing atypia. Although no correlation was found between atypia and Helicobacter pylori infection or intestinal metaplasia, enhanced proliferative ability (Ki-67) did correlate with atypia, as did nuclear 8-hydroxy-2’-deoxyguanosine levels. Interestingly, 4-hydroxynonenal levels in granulation tissue and the area ratio of granulation tissue within polyps also correlated with GHP atypia. In five cases of HPAGC, three cases exhibited caudal type homeobox transcription factor (CDX2)-positive cells and a mixed mucin phenotype, which is considered to be related to H. pylori infection. By contrast, two cases were CDX2 negative, with a gastric mucin phenotype, and H. pylori infection was not observed in the tumor or the surrounding mucosa. In these cases, a v-raf murine sarcoma viral oncogene homolog B1 (BRAF) mutation (V600E) was detected. All cancer samples showed high stemness and p53 protein accumulation, but no KRAS mutations. The molecular and phenotypic characteristics of the cases characterized by BRAF mutations may represent a novel subtype of HPAGC, reflecting a conserved pathway to oncogenesis that does not involve H. pylori infection. These findings are worthy of further investigation in a large-scale study with a substantial cohort of HPAGC patients to establish their clinical significance.

p53 abnormalities in splenic lymphoma with villous lymphocytes

Blood ◽  
2001 ◽  
Vol 97 (11) ◽  
pp. 3552-3558 ◽  
Author(s):  
Alicja M. Gruszka-Westwood ◽  
Rifat A. Hamoudi ◽  
Estella Matutes ◽  
Esperanza Tuset ◽  
Daniel Catovsky
Keyword(s):  
Flow Cytometry ◽  
Protein Expression ◽  
Direct Sequencing ◽  
P53 Protein ◽  
Protein Accumulation ◽  
Low Grade ◽  
Splenic Marginal Zone Lymphoma ◽  
Kaplan Meier ◽  
Splenic Lymphoma

The incidence and role of p53 abnormalities have not been reported in splenic lymphoma with villous lymphocytes (SLVL), the leukemic counterpart of splenic marginal zone lymphoma. Because p53 abnormalities correlate with progressive and refractory disease in cancer and isochromosome 17q has been described in SLVL, a low-grade lymphoma that behaves aggressively in a minority of patients, this study investigated p53 changes by molecular and immunophenotypic methods in samples from 59 patients. The p53 deletion was analyzed by fluorescence in situ hybridization, and p53 protein expression was assessed by immunocytochemistry in 35 of 59 cases and by flow cytometry in 20 of 35 patients. Ten patients (17%) had a monoallelic p53 loss, 3 (9%) of 35 nuclear protein expression by immunocytochemistry, and 2 (10%) of 20 by flow cytometry. Two patients had both deletion and protein expression. Direct sequencing of all p53 exons was used to delineate mutations in 9 of 11 patients with an identified abnormality. Mutations, both compromising p53 DNA binding, were identified in the 2 patients with deletion and protein accumulation. Kaplan-Meier analysis revealed a significantly worse survival for patients with p53 abnormalities. Although p53 abnormalities are infrequent in SLVL, they underlie a more aggressive disease course and poor prognosis.

scholarly journals Epithelial-Myoepithelial Carcinoma of the Minor Salivary Glands: Case Series with Comprehensive Review

Diagnostics ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2124
Author(s):  
Kohei Okuyama ◽  
Yasuyuki Michi ◽  
Yoshihisa Kashima ◽  
Hirofumi Tomioka ◽  
Hideaki Hirai ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Pleomorphic Adenoma ◽  
Salivary Gland Tumor ◽  
Case Series ◽  
Low Grade ◽  
Myoepithelial Carcinoma ◽  
Minor Salivary Glands ◽  
Comprehensive Review ◽  
Epithelial Myoepithelial Carcinoma

Epithelial-myoepithelial carcinoma (EMC) is a rare salivary gland tumor that is histologically characterized by biphasic tubular structures composed of inner ductal and outer clear myoepithelial cells, which is especially uncommon in the minor salivary glands (MSG). Because of its histologic variety, complexity, and heterogeneity, it is sometimes challenging to make the accurate diagnosis. Here, we report a literature review of EMC of the MSGs with our experience of two cases. Incisional biopsy was suggestive of pleomorphic adenoma in Case 1 and pleomorphic adenoma or a low-grade salivary gland carcinoma in Case 2. Both cases were performed intraoral tumor resection, and they have good postoperative courses and are alive with no evidence of local recurrence or metastasis at 31 and 16 months, respectively. Considering that the anatomy, structure, and size of salivary glands are quite different from MSGs, it might be difficult to predict EMCs of the MSG similarly to EMCs of the major salivary glands. This comprehensive review also reports the features of EMC of the MSG cases and the trends of diagnosis and discusses treatment strategy.

Immunohistochemical detection of p53 protein accumulation in head and neck cancer: Correlation with p53 gene alterations

1999 ◽  
Vol 30 (10) ◽  
pp. 1221-1225 ◽  
Author(s):  
David Taylor ◽  
Wayne M Koch ◽  
Marianna Zahurak ◽  
Keerti Shah ◽  
David Sidransky ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
P53 Protein ◽  
P53 Gene ◽  
Protein Accumulation ◽  
Immunohistochemical Detection ◽  
Gene Alterations
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