Apatinib Combined With Radiotherapy Enhances Antitumor Effects in an In Vivo Nasopharyngeal Carcinoma Model

Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) are highly expressed in nasopharyngeal carcinoma; therefore, blocking the binding of VEGF and VEGFR may be a potential way to treat nasopharyngeal carcinoma. Apatinib inhibits tumor angiogenesis. Previous studies have suggested that treatment with apatinib has an antitumor effect on nasopharyngeal carcinoma. This study will investigate the effect of apatinib combined with radiotherapy. In this study, nude mice injected with CNE-2 nasopharyngeal carcinoma cells were randomly divided into 6 groups. Therapeutic effects were assessed by evaluating tumor inhibition rate, phosphorylation of VEGFR-2, CD31, partial oxygen pressure, and tumor metabolism. We found that the tumor inhibition of mice in the treated groups was better compared to that of the control group. In mice treated with apatinib alone, angiogenesis was prevented, and the tumor tissue partial oxygen pressure was reduced, thereby achieving an antitumor effect. Moreover, the tumor inhibitory effect of combined treatment was stronger than treatment with either apatinib or radiotherapy alone. Compared with monotherapy treatment, combined treatment better resisted angiogenesis. Apatinib combined with radiotherapy to treat nasopharyngeal carcinoma has synergistic effects, which may be related to enhanced antiangiogenesis.

Download Full-text

Interaction of human hemoglobin with oxygen in the presence of benzene and use of computer for construction of saturation curves

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19861795 ◽

1986 ◽

Vol 51 (8) ◽

pp. 1795-1801

Author(s):

Hana Hájková ◽

Zdeněk Pavlíček ◽

Vítěz Kalous

Keyword(s):

Oxygen Saturation ◽

Oxygen Pressure ◽

Time Profile ◽

Partial Oxygen Pressure ◽

Human Hemoglobin ◽

Allosteric Interaction ◽

Partial Oxygen

The time profile of changes in the oxygen saturation curves of human hemoglobin in the presence of benzene was investigated. The partial oxygen pressure p50, necessary for a half saturation, decreases during the first 3.5 h of interaction of hemoglobin with benzene and did not change afterwards. The character of changes in Hill's coefficient was similar. The oxygen saturation was modeled both for hemoglobin alone and, using MWC as a model of allosteric interaction, also for hemoglobin and benzene in a computer.

Download Full-text

Mismatch between Tissue Partial Oxygen Pressure and Near-Infrared Spectroscopy Neuromonitoring of Tissue Respiration in Acute Brain Trauma: The Rationale for Implementing a Multimodal Monitoring Strategy

International Journal of Molecular Sciences ◽

10.3390/ijms22031122 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1122

Author(s):

Mario Forcione ◽

Mario Ganau ◽

Lara Prisco ◽

Antonio Maria Chiarelli ◽

Andrea Bellelli ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Near Infrared Spectroscopy ◽

Oxygen Pressure ◽

Near Infrared ◽

Optical Signal ◽

Brain Trauma ◽

Partial Oxygen Pressure ◽

Tissue Respiration ◽

The Brain ◽

Partial Oxygen

The brain tissue partial oxygen pressure (PbtO2) and near-infrared spectroscopy (NIRS) neuromonitoring are frequently compared in the management of acute moderate and severe traumatic brain injury patients; however, the relationship between their respective output parameters flows from the complex pathogenesis of tissue respiration after brain trauma. NIRS neuromonitoring overcomes certain limitations related to the heterogeneity of the pathology across the brain that cannot be adequately addressed by local-sample invasive neuromonitoring (e.g., PbtO2 neuromonitoring, microdialysis), and it allows clinicians to assess parameters that cannot otherwise be scanned. The anatomical co-registration of an NIRS signal with axial imaging (e.g., computerized tomography scan) enhances the optical signal, which can be changed by the anatomy of the lesions and the significance of the radiological assessment. These arguments led us to conclude that rather than aiming to substitute PbtO2 with tissue saturation, multiple types of NIRS should be included via multimodal systemic- and neuro-monitoring, whose values then are incorporated into biosignatures linked to patient status and prognosis. Discussion on the abnormalities in tissue respiration due to brain trauma and how they affect the PbtO2 and NIRS neuromonitoring is given.

Download Full-text

Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products

Parasite ◽

10.1051/parasite/2020045 ◽

2020 ◽

Vol 27 ◽

pp. 47

Author(s):

Jing Ding ◽

Xiaolei Liu ◽

Bin Tang ◽

Xue Bai ◽

Yang Wang ◽

...

Keyword(s):

Dendritic Cells ◽

Tumor Growth ◽

Antitumor Effect ◽

Trichinella Spiralis ◽

Critical Role ◽

Tumor Model ◽

Control Group ◽

Tumor Inhibition ◽

Significant Difference ◽

Secretory Products

Excretory/Secretory Products (ESPs) of the nematode Trichinella spiralis contain antitumor-active substances that inhibit tumor growth. Mature dendritic cells (DCs) play a critical role in the antitumor immunity of the organism. As pathogen-derived products, it ought to be discussed whether T. spiralis ESPs will reduce the antitumor effect of mature DCs from the host before it is applied to patients’ tumors. Therefore, the aim of this work was to evaluate the immunological effect of DCs stimulated by T. spiralis ESPs in H22 tumor-bearing mice. H22 tumor model mice in this study were randomly divided into four groups according to the treatment: PBS control group, ESP group, DCs group, and DCs stimulated with T. spiralis ESP (ESP+DCs group). The antitumor effect was evaluated by tumor inhibition rate and cytokine detection using ELISA. The results showed significant inhibition in tumor growth in the ESP+DCs, DCs and ESP groups when compared with the PBS control group (p < 0.01, p < 0.01, and p < 0.05, respectively). However, no significant difference was observed on tumor inhibition rates between the ESP+DCs and DCs groups. The decrease in IL-4, IL-6, and IL-10, and the increase in IFN-γ between the DCs and ESP+DCs groups were also not significant. Therefore, DCs stimulated by ESP did not reduce the antitumor effect of mature DCs, which demonstrated that the T. spiralis ESP would not affect the antitumor effect of mature DCs by modulating the immune response of the host, and that ESPs are safe in antitumor immunology when applied in a tumor model mice.

Download Full-text