Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe
2008 ◽
Vol 13
(4)
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pp. 302-308
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Keyword(s):
Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH2PO 4-Na2HPO4 buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm (ΔI580 nm) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. ( Journal of Biomolecular Screening 2008:302-308)
Keyword(s):
1985 ◽
Vol 48
(1)
◽
pp. 16-20
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1990 ◽
Vol 73
(2)
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pp. 257-259
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Keyword(s):
2012 ◽
Vol 77
(1)
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pp. 95-104
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