JC virus in cerebrospinal fluid samples of multiple sclerosis patients at the first demyelinating event

2007 ◽  
Vol 13 (5) ◽  
pp. 590-595 ◽  
Author(s):  
Roberto Alvarez-Lafuente ◽  
Marta García-Montojo ◽  
Virginia De Las Heras ◽  
Manuel Bartolomé ◽  
Rafael Arroyo
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Jc Virus ◽  
Neurological Diseases ◽  
Positive Sample ◽  
Viral Loads ◽  
Immunological Activation ◽  
Viral Particles ◽  
Multiple Sclerosis Patients

Objective To evaluate the possible involvement of JC virus (JCV) in the aetiology of multiple sclerosis (MS), through the comparison of DNA prevalences and viral loads of JCV in cerebrospinal fluid (CSF) of MS patients at the first demyelinating event and subjects suffering from other neurological diseases (OND). Methods Seventy-three CSF samples (43 from MS patients at the first demyelinating event, and 30 from patients with OND) were collected; all MS cases were followed up from 1 to 6.7 years after they were diagnosed with clinically definite MS. DNA was extracted and analysed by real-time PCR for the detection of JCV genomes. Results We found JCV DNA in the CSF of two MS patients (4.7%) with a mean viral load of 2.1 and 6.7 copies/mL of CSF. Among the patients of the OND group we did not find any positive sample. We did not find any difference in the course of the disease between MS patients with and without JCV genomes in their CSF along the follow up. Conclusion JCV seems to be only a bystander in the pathology of MS, and the presence of cell-free viral particles could be related to the immunological activation of the disease, mainly during relapses. Multiple Sclerosis 2007; 13: 590-595. http://msj.sagepub.com

scholarly journals Analysis of cerebrospinal fluid and cerebrospinal fluid cells from patients with multiple sclerosis for detection of JC virus DNA

2009 ◽  
Vol 15 (1) ◽  
pp. 28-35 ◽  
Author(s):  
E Iacobaeus ◽  
C Ryschkewitsch ◽  
M Gravell ◽  
M Khademi ◽  
E Wallstrom ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Copy Number ◽  
Jc Virus ◽  
Neurological Diseases ◽  
Csf Cells ◽  
Increased Risk ◽  
Low Copy Number ◽  
Patient At Risk

Objective 1) To determine whether JC virus (JCV) DNA was present in the cerebrospinal fluid (CSF) and blood from patients with multiple sclerosis (MS) in comparison with controls and 2) to find out if our clinical material, based on presence of JCV DNA, included any patient at risk for progressive multifocal leukoencephalopathy (PML). Methods The prevalence of JCV DNA was analyzed in CSF and plasma from 217 patients with MS, 86 patients with clinically isolated syndrome (CIS), and 212 patients with other neurological diseases (OND). In addition, we analyzed CSF cells, the first report of JCV DNA in CSF cells in a single sample, and peripheral blood cells in a subgroup of MS ( n = 49), CIS ( n = 14) and OND ( n = 53). Results A low copy number of JCV DNA was detected in one MS cell free CSF sample and in one MS CSF cell samples. None of these had any signs of PML or developed this disease during follow-up. In addition, two OND plasma samples were JCV DNA positive, whereas all the other samples had no detectable virus. Conclusion A low copy number of JCV DNA may occasionally be observed both in MS and other diseases and may occur as part of the normal biology of JC virus in humans. This study does not support the hypothesis that patients with MS would be at increased risk to develop PML, and consequently screening of CSF as a measurable risk for PML is not useful.

Silver staining of unconcentrated cerebrospinal fluid in agarose gel (Panagel) electrophoresis.

1984 ◽  
Vol 30 (5) ◽  
pp. 735-736 ◽  
Author(s):  
P D Mehta ◽  
S P Mehta ◽  
B A Patrick
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Silver Staining ◽  
Clinical Laboratory ◽  
Neurological Diseases ◽  
Agarose Gel ◽  
Oligoclonal Igg ◽  
Multiple Sclerosis Patients ◽  
Coomassie Brilliant ◽  
Oligoclonal Igg Bands

Abstract We subjected cerebrospinal fluid (CSF) from 20 patients with multiple sclerosis and 20 patients with other neurological diseases to agarose gel ( Panagel ) electrophoresis followed by staining with silver. Ten microliters of unconcentrated CSF from multiple sclerosis patients containing 0.4 to 0.8 microgram of immunoglobulin G was found to be optimum for detection of oligoclonal IgG bands, so identified by immunofixation. The band patterns for unconcentrated CSF stained with silver were almost identical to those for the same CSF concentrated 40-fold and stained with Coomassie Brilliant Blue. Silver staining thus enables the clinical laboratory to electrophorese unconcentrated CSF on commercially prepared ( Panagel ) plates.

T cells from multiple sclerosis patients recognize immunoglobulin G from cerebrospinal fluid

2003 ◽  
Vol 9 (3) ◽  
pp. 228-234 ◽  
Author(s):  
T Holmøy ◽  
B Vandvik ◽  
F Vartdal
Keyword(s):  
Multiple Sclerosis ◽  
T Cells ◽  
Cerebrospinal Fluid ◽  
T Cell ◽  
Mononuclear Cells ◽  
Neurological Diseases ◽  
Affinity Maturation ◽  
Peripheral Blood Mononuclear ◽  
Number Of Patients ◽  
Multiple Sclerosis Patients

Idiotopic sequences are created after V, D and J recombinations and by somatic mutations during affinity maturation of immuglobulin (Ig) molecules, and may therefore be potential immunogenic epitopes. Idiotope-specific T cells are able to activate and sustain the B cells producing such idiotopes. It is therefore possible that idiotope-specific intrathecal T cells could help maintain the persisting intrathecal synthesis of oligoclonal IgG observed in patients with multiple sclerosis (MS). This study was undertaken to examine T-cell responses to cerebrospinal fluid (CSF) IgG. Peripheral blood mononuclear cells (PBMC) from 14 of 21 MS patients and four of 17 control patients with other neurological diseases proliferated upon stimulation with autologous C SF IgG, while five and three, respectively, responded to serum IgG. By comparison, responses to myelin basic protein were recorded in only four MS and three control patients. Data from a limited number of patients indicate that the C SF IgG responsive cells were CD4+ and human leucocyte antigen DR restricted, that PBMC also respond to C SF IgG from other MS patients and that the C SF may contain T cells responding to autologous C SF IgG. This suggests that C SF IgG, or substances bound to this IgG, may represent T-cell immunogens, which could contribute to the intrathecal immune response in MS.

No evidence of JC virus reactivation in natalizumab treated multiple sclerosis patients: an 18 month follow-up study

2010 ◽  
Vol 81 (12) ◽  
pp. 1345-1350 ◽  
Author(s):  
L. Rinaldi ◽  
F. Rinaldi ◽  
P. Perini ◽  
M. Calabrese ◽  
D. Seppi ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Jc Virus ◽  
Virus Reactivation ◽  
Follow Up Study ◽  
Multiple Sclerosis Patients

Detection of JC virus DNA in cerebrospinal fluid from multiple sclerosis patients

1998 ◽  
Vol 4 (2) ◽  
pp. 49-54 ◽  
Author(s):  
P. Ferrante ◽  
E. Omodeo-Zorini ◽  
R. Caldarelli-Stefano ◽  
M. Mediati ◽  
E. Fainardi ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Jc Virus ◽  
Multiple Sclerosis Patients

Detection of JC virus DNA in cerebrospinal fluid from multiple sclerosis patients

1998 ◽  
Vol 4 (2) ◽  
pp. 49-54 ◽  
Author(s):  
P Ferrante ◽  
E Omodeo-Zorini ◽  
R Caldarelli-Stefano ◽  
M Mediati ◽  
E Fainardi ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
Healthy Subjects ◽  
Jc Virus ◽  
Control Groups ◽  
Peripheral Blood Mononuclear ◽  
Neurological Patients ◽  
Sequences Analysis ◽  
Multiple Sclerosis Patients

JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), has been proposed as a possible aetiopathogenic factor in multiple sclerosis (MS). We performed a study to search the LT region of JCV genome by nested PCR in cerebrospinal fluid (CSF), peripheral blood mononuclear cell (PBMC) and urine samples collected from 121 MS patients, 24 patients with other neurological disorders (OND), 30 non neurological patients (NND) and in PBMCs and urine of 40 healthy subjects. JCV DNA has been found in the CSF of 11 MS patients (9%) while all the CSFs from the 24 OND and the 30 NND cases were negative. No significant differences have been observed as regard to the frequency of JCV DNA detection in PBMCs and urine between the MS patients and the control groups. Nucleotide sequences analysis of seven JCV CSF isolates showed that five strains were identical the prototypal strain, while the other two had a base mutation (T→C) in 4286 nucleotide (nt). The finding of JCV DNA in the CSF of MS patients suggest that JCV could play a role in the triggering and/or in the maintenance of MS aetiopathogenic process, and therefore it should be taken in consideration when monitoring this disease.

Cerebrospinal fluid and serum JC virus antibody detection in multiple sclerosis patients treated with natalizumab

2013 ◽  
Vol 261 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
Jerry Lin ◽  
Peggy Bettin ◽  
John K. Lee ◽  
Joseph K. Ho ◽  
Saud A. Sadiq
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Jc Virus ◽  
Antibody Detection ◽  
Virus Antibody ◽  
Multiple Sclerosis Patients

Increase of CD4+TNFα+IL-2−T cells in cerebrospinal fluid of multiple sclerosis patients

2009 ◽  
Vol 15 (1) ◽  
pp. 120-123 ◽  
Author(s):  
N Shi ◽  
Y Kawano ◽  
T Matsuoka ◽  
FJ Mei ◽  
T Ishizu ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cells ◽  
Cerebrospinal Fluid ◽  
Peripheral Blood ◽  
Neurological Diseases ◽  
Significant Change ◽  
Multiple Sclerosis Patients

Intracellular production of TNFα and IL-2 after stimulation with phorbol myristate/ionomycin was flowcytometrically measured in CD4+ T cells from peripheral blood (PB) and cerebrospinal fluid (CSF) of 29 patients with multiple sclerosis (MS), and 16 with other inflammatory and 41 with other non-inflammatory neurological diseases. In CSF, the percentages of CD4+TNFα+IL-2−T cells were significantly higher in patients with MS than either of the controls, whereas no difference was found in CD4+TNFα+IL-2+T or CD4+TNFα−IL-2+T cells. The increase was more pronounced at relapse than in remission. No significant change was detected in PB. These findings suggested that CD4+TNFα+IL-2−T cells are intrathecally upregulated in MS.

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