Inhibitory Effects of Lycopene on the Adhesion, Invasion, and Migration of SK-Hep1 Human Hepatoma Cells

2006 ◽  
Vol 231 (3) ◽  
pp. 322-327 ◽  
Author(s):  
Eun-Sun Hwang ◽  
Hyong Joo Lee
Keyword(s):  
Cell Growth ◽  
Hepatoma Cell ◽  
Hepatoma Cells ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Human Hepatoma Cell ◽  
Invasion And Migration ◽  
And Migration

Lycopene, which is the predominant carotenoid in tomatoes and tomato-based foods, may protect humans against various cancers. Effects of lycopene on the adhesion, invasion, migration, and growth of the SK-Hep1 human hepatoma cell line were investigated. Lycopene inhibited cell growth in dose-dependent manners, with growth inhibition rates of 5% and 40% at 0.1 μM and 50 μM lycopene, respectively, after 24 hrs of incubation. Similarly, after 48 hrs of incubation, lycopene at 5 μM and 10 μM decreased the cell numbers by 30% and 40%, respectively. Lycopene decreased the gelatinolytic activities of both matrix metalloproteinase (MMP)-2 and MMP-9, which were secreted from the SK-Hep1 cells. Incubation of SK-Hep1 cells with 110 μM of lycopene for 60 mins significantly inhibited cell adhesion to the Matrigel-coated substrate in a concentration-dependent manner. To study invasion, SK-Hep1 cells were grown either on Matrigel-coated Transwell membranes or in 24-well plates. The cells were treated sequentially for 24 hrs with lycopene before the start of the invasion assays. Cell growth and death were assessed under the same conditions. The invasion of SK-Hep1 cells treated with lycopene was significantly reduced to 28.3% and 61.9% of the control levels at 5 μM and 10 μM lycopene, respectively (P < 0.05). In the migration assay, lycopene-treated cells showed lower levels of migration than untreated cells. These results demonstrate the antimetastatic properties of lycopene in inhibiting the adhesion, invasion, and migration of SK-Hep1 human hepatoma cells.

scholarly journals KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling

2019 ◽  
Vol 15 (5) ◽  
pp. 953-961 ◽  
Author(s):  
Li Li ◽  
Shijun Yu ◽  
Qiong Wu ◽  
Ning Dou ◽  
Yandong Li ◽  
...  
Keyword(s):  
Cell Growth ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Cell Growth And Migration ◽  
And Migration ◽  
Gsk 3Β

scholarly journals Effects of Fresh Royal Jelly on the Proliferation of Human Hepatoma Cell Line SMMC-7721

1970 ◽  
Vol 1 (2) ◽  
Author(s):  
Mingmin Wang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Royal Jelly ◽  
Hepatoma Cell ◽  
Inhibitory Effect ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

Objective: To investigate the effect of fresh royal jelly on the proliferation of human hepatoma cell line SMMC-7721. Methods: We found that the administration of fresh royal jelly could alleviate the condition of hepatocellular carcinoma patients in a certain extent. The human hepatocellular carcinoma cell line SMMC-7721 was cultured in vitro. MTT colorimetric method was used to treat fresh cells and serum containing human serum. The effect of SMMC-7721 proliferation was observed. Results: The aqueous solution of fresh royal jelly had a certain effect on the proliferation of hepatoma cell line SMMC-7721 in a dose-dependent manner. The serum containing fresh royal jelly could inhibit the proliferation of human hepatoma cell line SMMC-7721, and its inhibitory effect showed dose-dependent. Conclusion: The serum containing fresh royal jelly has a significant inhibitory effect on the proliferation of human hepatoma cell line SMMC-7721 and its anti-cancer effect may be derived from its metabolites or stimulating the formation of immune-reactive substances in the body, in which in the clinical treatment of liver cancer and research have a certain value.

scholarly journals Phorbol ester inhibits erythropoietin production in human hepatoma cells (Hep G2)

1992 ◽  
Vol 262 (5) ◽  
pp. C1204-C1210 ◽  
Author(s):  
A. Kurtz ◽  
K. U. Eckardt ◽  
C. Pugh ◽  
P. Corvol ◽  
D. Fabbro ◽  
...  
Keyword(s):  
Phorbol Ester ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Maximal Effect ◽  
Mrna Levels ◽  
Human Hepatoma ◽  
Low Oxygen ◽  
Hep G2 ◽  
Human Hepatoma Cells ◽  
Human Hepatoma Cell

Using the human hepatoma cell line Hep G2, we have studied a possible role of protein kinase C (PKC) activity for regulation of erythropoietin (EPO) production. During a 72-h incubation, EPO production by the cells was stimulated sevenfold by exposure to low oxygen tension (1%) and threefold by exposure to cobaltous chloride (100 microM). The phorbol ester phorbol 12-myristate-13 acetate (PMA) led to a concentration-dependent inhibition of basal and stimulated EPO formation (ED50 10 nM). This decrease of EPO production, which was apparent already after 1 h of incubation with PMA, reached its maximal effect after 24 h and held on for 72 h. It was paralleled by an inhibition of the increase of EPO mRNA levels in response to stimulation. A 24-h preincubation of the cells with PMA (100 nM) virtually blunted the effect of hypoxia on EPO formation. Recovery of EPO synthesis after removal of PMA took 48-72 h. The effect of PMA on EPO production was mimicked by phorbol 12,13-dibutyrate (ED50 1 microM) but not by 4 alpha-phorbol 12,13-didecanoate. The synthetic diacylglycerol analogues oleolyl-acetylglycerol and dioctanoylglycerol (2-200 microM) also had no effect on either basal or stimulated EPO production. Treatment with PMA caused a translocation of the alpha-isoenzyme of PKC from the cytosol to the membrane after 1 h and a disappearance of the membrane-bound form after 24 h of incubation. Staurosporine and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, two structurally different inhibitors of PKC activity, inhibited basal and stimulated EPO production with ED50 values of 9 nM and 50 microM, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Effect of lysosomotropic amines on the secretory pathway and on the recycling of the asialoglycoprotein receptor in human hepatoma cells.

1985 ◽  
Vol 101 (2) ◽  
pp. 531-539 ◽  
Author(s):  
G J Strous ◽  
A Du Maine ◽  
J E Zijderhand-Bleekemolen ◽  
J W Slot ◽  
A L Schwartz
Keyword(s):  
Golgi Complex ◽  
Intracellular Transport ◽  
Secretory Pathway ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Receptor Recycling ◽  
Human Hepatoma Cells ◽  
Human Hepatoma Cell ◽  
Response Characteristics

We studied the intracellular transport of secretory and membrane proteins in the human hepatoma cell line HepG-2 infected with vesicular stomatitis virus. Cells were pulse-labeled in the presence of [35S]methionine and chased in the presence of the lysosomotropic agent primaquine. At a concentration of 0.3 mM primaquine effectively inhibited the secretion of albumin and, to a lesser extent, that of orosomucoid and transferrin. The drug also prevented the budding of virus particles at the cell surface. The intracellular transport to the Golgi complex of the membrane protein VSV-G was not affected by primaquine as it acquires resistance to endo-beta-N-acetylglucosaminidase H at the same rate as in control cells. Addition of primaquine at various times after the initiation of the chase period indicates that the effect of primaquine occurs just before secretion. In confirmation of the biochemical data, immunocytochemical localization of albumin in cells treated with NH4Cl demonstrated that albumin accumulated in vesicles at the trans side of the Golgi complex. The effect of primaquine on secretion was also compared with its effect on receptor recycling. The dose-response characteristics of the effect of primaquine on receptor recycling are identical to those of the effects on protein secretion and virus budding. These results indicate that both processes involve the same transport mechanism, and/or that they occur via at least one identical intracellular compartment.

Isotopomer spectral analysis of cholesterol synthesis: applications in human hepatoma cells

1994 ◽  
Vol 266 (3) ◽  
pp. E384-E395 ◽  
Author(s):  
J. K. Kelleher ◽  
A. T. Kharroubi ◽  
T. A. Aldaghlas ◽  
I. B. Shambat ◽  
K. A. Kennedy ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Cholesterol Synthesis ◽  
Hepatoma Cell ◽  
Gas Chromatography Mass Spectrometry ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Unknown Parameters ◽  
Human Hepatoma Cell ◽  
Cholesterol Precursors

Cholesterol synthesis from 13C-labeled precursors produces a discrete spectrum of mass isotopomers detectable using gas chromatography-mass spectrometry. The isotopomer spectral analysis (ISA) method matches the observed spectrum of cholesterol isotopomers with a mathematical model to obtain the best fit of model spectrum to data spectrum. The model was based on multinomial probability expressions that simulate cholesterol synthesis as a condensation of mevalonate fragments. As many as four unknown parameters, representing fluxes between compartments, were included in the model. Models were developed to assess cholesterol synthesis from 13C-enriched precursors including mevalonate, acetate, acetoacetate or octanoate. Models were tested in the human hepatoma cell line, Hep G2, which readily incorporated the 13C substrates into cholesterol. The ISA approach was used to estimate the fractional amount of the cholesterol precursors derived from the 13C substrate and the fraction of total cellular cholesterol synthesized in the presence of the 13C substrate. The study demonstrated the feasibility of the ISA approach for a condensation biosynthesis that is not a simple polymerization and for models with more than two unknown parameters.

Vitronetcin promotes cell growth and inhibits apoptotic stimuli in a human hepatoma cell line via the activation of caspases

2014 ◽  
Vol 92 (5) ◽  
pp. 363-368 ◽  
Author(s):  
Wei Zhu ◽  
Yingzhi Liu ◽  
Konghe Hu ◽  
Wenxue Li ◽  
Jianling Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Tumor Cell ◽  
Hepatoma Cell ◽  
Hepatoma Cells ◽  
Signaling Molecules ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Tumor Cell Growth ◽  
Induced Apoptosis

This study sought to understand the effects of vitronectin (VTN) on the growth of SMMC-7721 hepatoma cells. In addition, this study examined how VTN inhibits the induction of apoptosis in SMMC-7721 cells by 3,3′-diindolylmethane (DIM), a metabolite of natural phytochemicals, and preliminarily investigated the signaling molecules involved in this process. A cell proliferation reagent was used to observe the effects of VTN on cell proliferation rates. Laser scanning confocal microscopy was performed to observe the effects of VTN on the morphology of tubulin, a component of the cytoskeleton. Flow cytometry and Western blotting assays were used to observe the inhibitory effects of VTN on DIM-induced apoptosis in SMMC-7721 cells and changes in the expression levels of the signaling molecules involved in this process. VTN promoted tumor cell growth in a concentration-dependent manner and inhibited apoptosis caused by the effects of apoptosis-inducing agents. Under in vitro experimental conditions, VTN contributed to the growth of SMMC-7721 hepatoma cells and protected them from the effects of an apoptosis-inducing agent. These findings suggest that during hepatocellular carcinogenesis, VTN may promote tumor cell growth and inhibit chemically induced apoptosis.

scholarly journals Immunodetection and molecular cloning of a bile-salt-dependent lipase isoform in HepG2 cells

1999 ◽  
Vol 342 (1) ◽  
pp. 179-187 ◽  
Author(s):  
Alain VÉRINE ◽  
Nadine BRUNEAU ◽  
Anne VALETTE ◽  
Josette LE PETIT-THEVENIN ◽  
Eric PASQUALINI ◽  
...  
Keyword(s):  
Bile Salt ◽  
Molecular Mass ◽  
Hepg2 Cells ◽  
Hepatoma Cell ◽  
Pancreatic Enzyme ◽  
Hepatoma Cell Line ◽  
Human Pancreas ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Human Hepatoma Cell

In this article, we report the nucleotide sequence of the cDNA encoding an isoform of bile-salt-dependent lipase (BSDL) expressed by human hepatoma cells. The BSDL is a 100-kDa glycoprotein normally expressed by the human pancreas. Using a polyclonal antibody raised against an internal peptide located between Ile327 and Glu350 of the human pancreatic BSDL, we have immunodetected an isoform of human pancreatic BSDL, with an apparent molecular mass of about 62 kDa. This isoform of BSDL was mainly associated with the cytosol of a human hepatoma cell line (HepG2), the remaining protein being found in the microsome fraction. In addition, esterolytic activity on p-nitrophenyl hexanoate measured in microsomes and cytosol appeared very low and was weakly stimulated by bile salts, such as taurocholate. In contrast to human pancreatic BSDL, which is secreted as a component of pancreatic juice, this isoform appeared to be retained in the HepG2 cells. Reverse transcription, followed by PCR and amplification, performed on RNA extracted from HepG2 cells using specific primers hybridizing to the sequence coding for the entire normal human pancreatic BSDL, allowed us to amplify a 1.7-kb transcript that appeared to be 0.5 kb shorter than the transcript of the pancreatic enzyme (2.2 kb). From the sequence of the transcript thus obtained, a protein with a molecular mass of 62 kDa might be predicted, which is in good agreement with the size of the isoform of BSDL immunodetected in HepG2 cells. The N-terminal amino-acid sequence, deduced from the 1.7-kb transcript sequence, matched that of the pancreatic BSDL. However, the C-terminal domain appeared truncated, bearing only a single mucin-like sequence compared with sixteen for the human pancreatic BSDL. The actual intracellular function of this human BSDL hepatoma isoform remains to be elucidated.

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