Mechanism of Azithromycin Treatment on Gingival Overgrowth

2008 ◽  
Vol 87 (11) ◽  
pp. 1075-1079 ◽  
Author(s):  
J.-Y. Kim ◽  
S.-H. Park ◽  
K.-S. Cho ◽  
H.-J. Kim ◽  
C.-K. Lee ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Renal Transplant ◽  
Cyclosporin A ◽  
Cyclosporine A ◽  
Type I Collagen ◽  
Culture Media ◽  
Mrna Level ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Gingival Overgrowth

Azithromycin is effective for the remission of cyclosporine A-induced gingival overgrowth (CIGO) in persons who have undergone renal transplant. To explain its mechanism in alleviating the clinical symptoms of these indivduals, we examined the effect of azithromycin on cell proliferation and collagen turnover modified by cyclosporin A in human gingival fibroblasts from healthy persons and from persons who had undergone renal transplant. Cyclosporin A-induced proliferation of renal transplant fibroblasts and normal fibroblasts was inhibited by azithromycin. Azithromycin elevated the reduced metalloproteinase (MMP)-1 and MMP-2 activities in cyclosporine A-treated renal transplant fibroblasts and normal fibroblasts. In cyclosporine A-treated renal transplant fibroblasts, azithromycin blocked the accumulation of total collagen in culture media and the increase in type I collagen mRNA level, but recovered the reduced MMP-2 mRNA level to the control. These results suggest that azithromycin may improve CIGO by blocking cyclosporine A-induced cell proliferation and collagen synthesis, and by activating MMP-2 in gingival fibroblasts of persons with cyclosporine A-induced gingival overgrowth.

scholarly journals Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Kotaro Tanimoto ◽  
Satoru Ohkuma ◽  
Yuki Tanne ◽  
Ryo Kunimatsu ◽  
Naoto Hirose ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Caspase 3 ◽  
Type I Collagen ◽  
Pcr Analysis ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Cultured Fibroblasts ◽  
Proliferation And Apoptosis ◽  
Young Subjects

The purpose of this study was to investigate the effects of basic fibroblast growth factor (bFGF) treatment on the proliferation and apoptosis of cultured gingival fibroblasts (GFs). Human GFs were isolated from the palatal gingival tissues of 16 healthy volunteers ranging in the age from 9 to 35 years old. Cultured GFs were subjected to the analyses for cell proliferation by ELISA assay, gene expression by RT-PCR analysis, and apoptosis potency by caspase-3 assay. The cell proliferation activity and gene expression of type-I collagen and caspase-3 activity were enhanced significantly by the treatment with bFGF in cultured GFs. Furthermore, the activity of caspase-3 in cultured GFs from young subjects was significantly higher than that in GFs from adults. It is shown that bFGF significantly enhances the gene expression of type-I collagen in cultured fibroblasts from human gingival tissues. It also demonstrated that bFGF modulates the apoptosis of periodontal fibroblasts, and the effect is higher in young subjects, indicating a significant role of bFGF in the prevention of scar formation during wound healing.

Cyclosporin A decreases the degradation of type I collagen in rat gingival overgrowth

2000 ◽  
Vol 182 (3) ◽  
pp. 351-358 ◽  
Author(s):  
Masatoshi Kataoka ◽  
Yasuki Shimizu ◽  
Kenji Kunikiyo ◽  
Yoji Asahara ◽  
Kikuji Yamashita ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Type I Collagen ◽  
Type I ◽  
Gingival Overgrowth

Cyclosporin-A increases type I procollagen production and mRNA level in human gingival fibroblasts in vitro

1992 ◽  
Vol 21 (4) ◽  
pp. 181-185 ◽  
Author(s):  
G. P. Schincaglia ◽  
F. Forniti ◽  
R. Cavallini ◽  
R. Piva ◽  
G. Calura ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Mrna Level ◽  
Human Gingival Fibroblasts ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Type I Procollagen

Relationship between IL-1A polymorphisms and gingival overgrowth in renal transplant recipients receiving Cyclosporin A

2006 ◽  
Vol 33 (11) ◽  
pp. 771-778 ◽  
Author(s):  
Nagihan Bostanci ◽  
Tunç Ilgenli ◽  
Demet Can Pirhan ◽  
Fiona M. Clarke ◽  
Wagner Marcenes ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Cyclosporin A ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Gingival Overgrowth

Cellular Compatibility of a New Tantalum-Niobium Scaffold Material

2020 ◽  
Author(s):  
Jianan Ouyang ◽  
Zhenhan Deng ◽  
Kang Chen ◽  
Jianyi Xiong ◽  
Ying Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Type I Collagen ◽  
Material Surface ◽  
Control Group ◽  
Type I ◽  
Scaffold Material ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Porous Tantalum ◽  
Significant Difference

Abstract [Objective] To determine the cellular compatibility of porous tantalum-niobium (Ta-Nb) material. [Method] Rabbit osteoblasts were co-cultured with porous Ta-Nb material. The cell proliferation was detected by CCK-8 method, and the cell adhesion was observed under scanning electron microscope (SEM). The expressions of type-I collagen and osteocalcin were detected by RT-PCR assay. [Results] CCK-8 detection indicated that the cell proliferation on the porous Ta-Nb material showed no difference from that of the control group (P>0.05). SEM revealed that a large amount of cells adhered onto the surface and in the pores of the material. The number of cells on the material surface increased obviously over time. RT-PCR assay showed that with the prolonging of the time of co-culture, the expression of type-I collagen was enhanced (P<0.05), while the osteocalcin expression exhibited no significant difference (P>0.05[Conclusion] Porous Ta-Nb scaffold material can be used to promote the adhesion, growth and differentiation of osteoblasts with satisfactory cellular compatibility.

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